FastQCFastQC Report
Fri 10 Feb 2017
SRR3557198.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557198.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16732
Sequences flagged as poor quality0
Sequence length125
%GC44

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2351.4044943820224718No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.6992589050920393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.5498446091322018No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT590.3526177384652164No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA580.34664116662682287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT420.25101601721252687No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC370.2211331580205594No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.2151565861821659No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT340.20320344250537892No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG260.15539086779823094No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT250.14941429595983743No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA240.14343772412144393No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.14343772412144393No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT240.14343772412144393No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA240.14343772412144393No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC240.14343772412144393No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC220.13148458044465694No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC210.12550800860626343No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG200.11953143676786994No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC200.11953143676786994No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA190.11355486492947646No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT190.11355486492947646No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA190.11355486492947646No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA180.10757829309108295No Hit
ACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAAC170.10160172125268946No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG170.10160172125268946No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACAA250.002027286271.347915
ACAATGG250.002027286271.347918
CAATGGG300.004175971359.4565859
GAAACCT150.00421283759.456585106-107
CATGGGG708.6500404E-750.9627884
ACATGGG2650.035.898323
AACGCAG3500.035.6739546
ACGCAGA3750.033.295697
CGCAGAG3800.032.857598
GTATCAA3750.031.9012071
TATCAAC3950.031.609832
ATCAACG3950.031.609833
GTACATG3050.031.3782331
GCAGAGT4000.031.2147089
TACATGG3050.031.190342
TCAACGC4150.030.0864664
CAACGCA4200.029.7282965
GAGTACT2000.028.24187912-13
AACCCAG450.00943311826.42514838-39
AGTACTT2250.025.10389312-13
Produced by FastQC (version 0.11.5)