FastQCFastQC Report
Fri 10 Feb 2017
SRR3557179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4068946
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT443061.0888814941264888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT235240.578134976477938No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT172860.4248274614605355No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA116750.28692934238989654No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT100790.24770542543449828No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90730.22298157803028107No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA56640.13920066769133824No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG55660.136792181562498No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49860.12253787590201491No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA48740.1197853203261975No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG46790.11499292445758681No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44530.10943866052781236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8900.046.8922461
TAACGCA9200.035.777194
TACAACG7100.035.5972252
AGATTAC21650.032.03542
TATCACG7600.031.7085442
GATTACT22550.030.4961743
CATGGGG112400.029.44074
TCACGCA8850.029.222374
AACGCAG581700.029.1004336
TTCAACG24100.029.022584
AAACGCA12800.028.929215
TAGTACT15550.028.3489445
CATGGGT47050.028.1079354
ACAACGC9550.027.6958793
ACATGGG520850.027.0609193
ACGCAGA639050.026.4520957
GTATCAA618000.026.4131451
CGCAGAG642100.026.33568
CATGGGC112200.026.2976464
GTATAAC13500.026.1246341