FastQCFastQC Report
Fri 10 Feb 2017
SRR3557160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1052343
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115981.1021121440442898No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50770.4824472629171287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50010.4752252830113376No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA30970.2942956811609903No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23230.22074551738359072No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21080.20031491633431303No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC19680.18701126913943458No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG19280.183210227083755No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC18630.1770335337432757No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT18130.17228223117367628No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT15520.14748043176036713No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG14880.1413987644712798No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG13990.1329414458973928No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA13510.1283801954305773No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11910.11317602720785905No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA11880.11289094905368308No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA11660.1108003759230593No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC11160.10604907335345987No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT11110.10557394309649991No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3000.061.495761
GACCGTT1950.051.867497
TACAACG2400.047.1069682
ATAGTAC4050.047.0151444
GTATAAC2500.045.2291371
TCAAACG758.18531E-539.669034
TAGTACT4900.038.859465
ACAACGC2950.038.324313
TAACGCA2900.036.933234
GTACAAC3400.036.7574271
ACGGTAC3900.036.6175653
GCATAGT5100.036.168822
ATAACGC3650.035.8651473
TTAGGAC3150.034.002023
GTACTGG9300.032.6356281
GCGTAAT750.003906470331.7397481
TACTGGT8850.031.600752
CATGGGC39000.031.5826474
GTATCAA167600.031.4604131
TCACGCA1901.0750227E-931.3176544
Produced by FastQC (version 0.11.5)