FastQCFastQC Report
Fri 10 Feb 2017
SRR3557159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9130541
Sequences flagged as poor quality0
Sequence length125
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5165125.656970381054091No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2659562.912817542794014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1655681.8133427143035663No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220761.3370073032912289No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1013781.1103175594962007No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA918481.0059425832489004No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT836520.9161779132255142No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA671740.7357066793742014No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA559330.6125923973179683No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAA466770.5112183385409473No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA392670.43006213980091645No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370760.4060657522922245No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330140.3615776984080133No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA305320.3343942051188424No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA276640.3029831419627818No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC242010.2650554879497283No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA222730.24393954312236263No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200350.21942839969723588No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA198760.21768699138419073No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197970.21682176335443867No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196930.2156827289861576No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA191710.20996565263766953No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA191700.2099547003841284No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA184260.20180622374950183No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183140.20057957135289134No Hit
GTATCAACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGT163480.17904744089096145No Hit
GTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAAAAAA159210.1743708286288841No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA157370.17235561397730978No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC155210.16998992721241818No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA143790.15748245366840805No Hit
GTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAA129280.14159073378017797No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA126100.13810791715408757No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT125900.13788887208326428No Hit
GTATCAACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAA125020.1369250737716418No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCT121080.13260988587642286No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA118500.12978420446280237No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAA116570.12767041952935756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG116550.12764851502227523No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC111480.1220957224769047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA110040.12051859796697699No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA108140.11843766979415568No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107680.1179338661312621No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA103710.11358582147541968No Hit
GCTGCTAGCTGGAGCCACCCGCAGTTCGAAAAAGTGAGCAAGGGCGAGGA101390.11104489865386948No Hit
TATCAACGCAGAGTACATGGGACTGCTGCTAGCTGGAGCCACCCGCAGTT100430.10999348231391765No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC98690.10808779019775498No Hit
AGCTGGAGCCACCCGCAGTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA97360.10663114047678007No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACA94480.10347689145692462No Hit
ACGCAGAGTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA93700.10262261568071376No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA92430.10123167948098584No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACACAAAAAAAAAA91460.10016931088749287No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6250.063.7848244
TTAGGAC6450.063.651993
CTTAGGA6950.058.2165832
TTGGACT11100.056.2845154
TTTGGAC13550.046.5467263
GACCGTT8450.046.4688877
TGGACTG13650.046.204215
AACGCAG3779550.045.9612246
GCCTAGT8600.044.2912861
GTATCAA4107050.043.252131
TATCAAC4349900.040.540792
ATCAACG4434900.039.51963
TAGTTCC10100.039.470814
CGCAGAG4421400.039.250178
ACGCAGA4434100.039.170867
TCAACGC4508600.038.9131854
GCAGAGT4565450.037.99619
CAACGCA4629400.037.9299475
CTAGTTC10200.037.9171563
GGTCATA8000.037.197761
Produced by FastQC (version 0.11.5)