FastQCFastQC Report
Fri 10 Feb 2017
SRR3557147.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557147.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35155622
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2212190.6292563960324753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1054970.3000857160200437No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT645990.18375154904100405No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380840.10832975732871401No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC372770.10603424965713877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1928350.049.2392121
AACGCAG2303200.041.9455156
TATCAAC2417850.039.134932
ATCAACG2422650.038.9271743
TCAACGC2469400.038.6120344
CAACGCA2486200.038.4115645
ACGCAGA2628900.036.606247
CGCAGAG2686450.035.901518
GCAGAGT2880400.033.506829
GTACATG2546950.030.5167331
ACATGGG2502150.030.20893
TACATGG2581250.030.0210782
CATGGGT436000.027.767914
AGAGTAC2783700.027.12761310-11
CATGGGG1002300.026.9966834
AGTACTT1515750.024.1232612-13
GGTATCA1111900.023.366861
ACGGTAC72100.023.363073
GTACTGG236200.022.680071
GAGTACT1319150.022.32764612-13
Produced by FastQC (version 0.11.5)