FastQCFastQC Report
Fri 10 Feb 2017
SRR3557139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627121
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147410.9059559799179041No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82970.5099190533463707No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68660.42197230568593236No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29850.18345285937554737No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29100.1788434910495286No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA24810.15247790422470117No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT20630.12678835808768985No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19820.12181024029558957No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18810.11560295761655094No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC18760.11529566639481637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3000.063.529152
CGCATAG4750.055.1751441
TACAACG2900.051.3435972
TAACGCA4350.046.5515334
TCACGCA4550.044.505314
ACAACGC3650.042.425293
ATCACGC4850.040.524393
ATAGTAC6750.039.705724
GTATAAC5850.036.6548161
TAGTACT7600.035.2638635
AGATTAC8600.034.627082
ACGCATA1805.966285E-1033.0911521
CATGGGC38300.031.256594
AAACGCA5350.031.1698845
AACGCAG188450.030.7161626
GATTACT9550.030.558853
GTATCAC7900.030.1590231
GCATAGT8800.029.779292
TACTGGT10800.029.7792872
GTACTGG12050.029.658461
Produced by FastQC (version 0.11.5)