FastQCFastQC Report
Fri 10 Feb 2017
SRR3557123.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557123.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4786386
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT622181.2998951609836733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338740.7077155916802363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241100.5037203434908927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124390.2598829262830035No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGG119990.25069018670871923No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT110530.23092579662400817No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA106500.22250608287756152No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT102080.21327155812339416No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT100940.21088980287005685No Hit
GTCTACAGCATGGTGAGAGCCCATTCACGTTCGGCTCGGGGACAAAGTTG99520.20792305509835604No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG99200.2072544922202263No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC86940.18164017695187976No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC78280.16354719406249307No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC78180.16333826816307753No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT77610.1621473905364089No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG75590.15792708736821476No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68410.14292620779017823No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT67370.1407533784362565No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT66960.13989678224865273No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT66160.13822537505332833No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT62200.12995190943647253No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG61920.12936691691810898No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60240.12585696180792774No Hit
CCTAAGACCCTGATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATC58500.12222165115809715No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA57240.11958918482546121No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT56330.11768795914077972No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT56100.11720742957212393No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA55720.11641351115434485No Hit
CTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCA55610.11618369266498774No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT53990.11279909309445582No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC53850.11250659683527404No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT51170.10690738273093729No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA50420.10534043848532065No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT50120.104713660787074No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA49700.1038361720095287No Hit
GATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTG49610.1036481387000547No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA49350.10310493136157425No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG48050.1003888946691721No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG10500.060.0915451
ATAGTAC11550.056.1442574
AGATTAC12500.049.9735342
ACGGTAC10650.049.7165763
TACAACG5650.049.4891782
TAACGCA9200.047.205854
GCATAGT13700.046.0305442
TAGTACT14550.043.7494435
AACGCAG520750.043.423056
AGTGTAC10600.043.2161063
ACTGGTT31400.042.0614363
GTACTGG32550.041.877551
CATAGTA15300.041.6057323
GTATCAA528500.041.1998371
GATTACT15300.040.4392133
ATCTACG5600.040.369782
GTACAAC8150.040.1700061
GACCGTT5350.040.031377
AAACGCA12350.039.981855
GTACATG496250.039.223291
Produced by FastQC (version 0.11.5)