##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557058.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3899851 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.5260088141829 33.0 32.0 34.0 27.0 34.0 2 31.82684364094936 33.0 32.0 34.0 27.0 34.0 3 32.007742347079414 33.0 32.0 34.0 27.0 34.0 4 31.90537971835334 33.0 32.0 34.0 27.0 34.0 5 32.04621638108738 33.0 32.0 34.0 28.0 34.0 6 35.43974910836337 38.0 36.0 38.0 29.0 38.0 7 35.7610906160261 38.0 36.0 38.0 31.0 38.0 8 35.972414330701355 38.0 37.0 38.0 31.0 38.0 9 36.08955290855984 38.0 37.0 38.0 32.0 38.0 10-11 36.08313471463397 38.0 37.0 38.0 32.0 38.0 12-13 36.04435105341204 38.0 37.0 38.0 31.5 38.0 14-15 36.027217962942686 38.0 37.0 38.0 31.0 38.0 16-17 36.01045706618022 38.0 37.0 38.0 31.0 38.0 18-19 35.972763061973396 38.0 37.0 38.0 31.0 38.0 20-21 35.94061555172236 38.0 37.0 38.0 31.0 38.0 22-23 35.8463922083177 38.0 37.0 38.0 31.0 38.0 24-25 35.78530961823926 38.0 37.0 38.0 30.0 38.0 26-27 35.551866853374655 38.0 37.0 38.0 29.0 38.0 28-29 35.55876673237003 38.0 37.0 38.0 29.0 38.0 30-31 35.49530123073933 38.0 37.0 38.0 29.0 38.0 32-33 35.452934868537284 38.0 37.0 38.0 28.5 38.0 34-35 35.25878783574039 38.0 36.0 38.0 27.5 38.0 36-37 35.11939033055366 38.0 36.0 38.0 27.0 38.0 38-39 34.94993629243784 38.0 36.0 38.0 25.5 38.0 40-41 34.70644391285718 38.0 36.0 38.0 24.5 38.0 42-43 34.242776326582735 38.0 35.0 38.0 16.0 38.0 44-45 33.94648731451535 38.0 34.5 38.0 16.0 38.0 46-47 33.50921394176342 38.0 34.0 38.0 15.0 38.0 48-49 33.11833054647472 38.0 33.0 38.0 15.0 38.0 50-51 32.78974581336569 38.0 31.5 38.0 15.0 38.0 52-53 32.547395913331044 38.0 30.0 38.0 15.0 38.0 54-55 32.37056749091183 38.0 29.0 38.0 15.0 38.0 56-57 32.15953391552652 38.0 27.5 38.0 15.0 38.0 58-59 32.05369282057187 38.0 27.0 38.0 15.0 38.0 60-61 31.93970641442455 38.0 26.5 38.0 15.0 38.0 62-63 31.839576691519753 38.0 26.0 38.0 15.0 38.0 64-65 31.664474360687116 38.0 26.0 38.0 15.0 38.0 66-67 31.57325728598349 38.0 25.5 38.0 15.0 38.0 68-69 31.428258028319544 38.0 25.0 38.0 15.0 38.0 70-71 31.29868423178219 38.0 25.0 38.0 15.0 38.0 72-73 31.101262202068746 38.0 25.0 38.0 14.0 38.0 74-75 30.99058015293405 38.0 24.0 38.0 14.0 38.0 76-77 30.900528250951126 38.0 24.0 38.0 14.0 38.0 78-79 30.749260548672247 38.0 23.5 38.0 14.0 38.0 80-81 30.575637120495117 38.0 22.5 38.0 14.0 38.0 82-83 30.45815635007594 37.5 22.0 38.0 14.0 38.0 84-85 30.363284135727234 37.0 21.0 38.0 14.0 38.0 86-87 30.32640298308833 37.0 21.0 38.0 13.5 38.0 88-89 30.218718099742784 37.0 21.0 38.0 13.5 38.0 90-91 30.138690811520746 37.0 21.0 38.0 13.0 38.0 92-93 30.073433574769908 37.0 21.0 38.0 13.0 38.0 94-95 30.049872418202646 37.0 21.0 38.0 13.0 38.0 96-97 29.998153390988527 37.0 21.0 38.0 13.0 38.0 98-99 29.908777284055212 37.0 15.0 38.0 13.0 38.0 100-101 29.824162128245412 37.0 15.0 38.0 13.0 38.0 102-103 29.693733683671503 36.0 15.0 38.0 13.0 38.0 104-105 29.628209770065574 36.0 15.0 38.0 13.0 38.0 106-107 29.459871543810266 36.0 15.0 38.0 13.0 38.0 108-109 29.334986387941488 36.0 15.0 38.0 13.0 38.0 110-111 29.151999012269954 35.0 15.0 38.0 13.0 38.0 112-113 28.98832852844891 35.0 15.0 38.0 13.0 38.0 114-115 28.839524766459025 35.0 14.5 38.0 13.0 38.0 116-117 28.653062770859705 35.0 14.0 38.0 13.0 38.0 118-119 28.53401835095751 34.5 14.0 38.0 13.0 38.0 120-121 28.282996965781514 34.0 14.0 38.0 13.0 38.0 122-123 28.192768390382096 34.0 14.0 38.0 13.0 38.0 124-125 26.958453156287252 30.0 14.0 37.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.4120916471156697 1101 2 -0.33971372275413003 1101 3 -0.425306682531712 1101 4 -0.3861649891697212 1101 5 -0.4997850399764836 1101 6 0.07636675214365596 1101 7 0.1681072896314575 1101 8 0.02987418480581283 1101 9 0.04972128611622395 1101 10-11 -0.047571306753589226 1101 12-13 0.08459788716225347 1101 14-15 0.03120769360158704 1101 16-17 0.0687420049506855 1101 18-19 -0.023648713251652964 1101 20-21 0.047127654280004094 1101 22-23 0.04086987998599767 1101 24-25 0.004829176420493297 1101 26-27 0.20676375328527996 1101 28-29 0.10520536167010874 1101 30-31 -0.07947332035450927 1101 32-33 0.07041516568988015 1101 34-35 0.04365438161662638 1101 36-37 0.11888636007361697 1101 38-39 0.06245603371954189 1101 40-41 0.2032535205520034 1101 42-43 0.13000436914563807 1101 44-45 0.1769200644004698 1101 46-47 0.2903588806989248 1101 48-49 0.3015948404385611 1101 50-51 0.14607004352730968 1101 52-53 0.13071616490368143 1101 54-55 0.10261256453186007 1101 56-57 0.1791078046436354 1101 58-59 0.07704045072748755 1101 60-61 0.1437239134366699 1101 62-63 0.05316743063641738 1101 64-65 0.03445578148330597 1101 66-67 -0.04760533241349307 1101 68-69 -0.12627090487104553 1101 70-71 -0.13663021214622617 1101 72-73 -0.027659102736826924 1101 74-75 -0.1836638717568988 1101 76-77 -0.193113813342066 1101 78-79 -0.1719253616537948 1101 80-81 0.01465253292760238 1101 82-83 0.002905457703448633 1101 84-85 -0.06938662587532818 1101 86-87 0.0453385908440751 1101 88-89 -0.11113881569152895 1101 90-91 -0.08101367853479857 1101 92-93 -0.09160873731658015 1101 94-95 -0.21397071336925677 1101 96-97 -0.08455381096525372 1101 98-99 -0.23221340692029457 1101 100-101 -0.12127622627083312 1101 102-103 -0.1001193656427084 1101 104-105 -0.21541849263978463 1101 106-107 -0.20372403227750624 1101 108-109 -0.46424410124521387 1101 110-111 -0.5546600701712237 1101 112-113 -0.5805192424061687 1101 114-115 -0.35281396976853685 1101 116-117 -0.43012818698663224 1101 118-119 -0.5386717098519718 1101 120-121 -0.7320805355762339 1101 122-123 -0.5369492571792698 1101 124-125 -0.5432633765661912 1102 1 -0.5231637503639099 1102 2 -0.7560443645449233 1102 3 -0.4761989873948771 1102 4 -0.3821899486294065 1102 5 -0.5032284590713907 1102 6 -0.1107761169403787 1102 7 0.031529059362469525 1102 8 0.034295168807020104 1102 9 -0.0909093038498412 1102 10-11 -0.21342048006552972 1102 12-13 -0.11031179570381511 1102 14-15 -0.04382406994389498 1102 16-17 -0.08187801109826864 1102 18-19 -0.0710618650312469 1102 20-21 -0.1691804186758432 1102 22-23 -0.030495074792739274 1102 24-25 -0.14695167183334235 1102 26-27 -0.07764891368230309 1102 28-29 -0.1815878994429525 1102 30-31 -0.13942993803845383 1102 32-33 -0.28397635344347094 1102 34-35 -0.24164787359510598 1102 36-37 -0.1889928799417646 1102 38-39 -0.10969109836665325 1102 40-41 -0.22472243110053114 1102 42-43 -0.09884131853512201 1102 44-45 -0.10320891231183538 1102 46-47 -0.14391126825538691 1102 48-49 -0.15770435544220618 1102 50-51 -0.2900722738740953 1102 52-53 -0.13577622020422808 1102 54-55 -0.09024920553350313 1102 56-57 -0.11167240833930236 1102 58-59 -0.17468919970633578 1102 60-61 -0.1871510886530352 1102 62-63 -0.1124531287363375 1102 64-65 -0.159545505776169 1102 66-67 -0.2724452608619252 1102 68-69 -0.41174493399320156 1102 70-71 -0.2958857331519589 1102 72-73 -0.3903937768261301 1102 74-75 -0.4042840549824014 1102 76-77 -0.48187056309548026 1102 78-79 -0.3384250607359931 1102 80-81 -0.33721775478325 1102 82-83 -0.2713990878270849 1102 84-85 -0.3213718474678551 1102 86-87 -0.36393720031897203 1102 88-89 -0.5406043104505471 1102 90-91 -0.5440852133827505 1102 92-93 -0.5698379614501867 1102 94-95 -0.6398128652658741 1102 96-97 -0.6224941122174066 1102 98-99 -0.5810525747154003 1102 100-101 -0.5710087437825777 1102 102-103 -0.472691753091091 1102 104-105 -0.46097932818820553 1102 106-107 -0.6291988136941633 1102 108-109 -0.6647537807677573 1102 110-111 -0.9212084594251166 1102 112-113 -0.6326628637847094 1102 114-115 -0.7647380465360101 1102 116-117 -0.6603181831415696 1102 118-119 -0.6114706215325967 1102 120-121 -0.9702678401886331 1102 122-123 -1.058340124324065 1102 124-125 -1.126577066654292 1103 1 -0.5378834967025234 1103 2 -0.29353935792335406 1103 3 -0.4063331871666094 1103 4 -0.39614977627890013 1103 5 -0.4587714725053118 1103 6 -0.12726296361113043 1103 7 -0.020107478320234407 1103 8 -0.003239989112145736 1103 9 -0.16822771828765326 1103 10-11 -0.10666145726880671 1103 12-13 -0.09828802443195173 1103 14-15 -0.07437045831491673 1103 16-17 -0.0428621343900204 1103 18-19 -0.0473744784821335 1103 20-21 -0.07009449668207424 1103 22-23 0.01589294758337445 1103 24-25 -0.07516132069485337 1103 26-27 0.006799613227379098 1103 28-29 -0.053225862409014724 1103 30-31 -0.13753118726723557 1103 32-33 -0.09735495277205786 1103 34-35 0.032249609761713316 1103 36-37 -0.020025607906212883 1103 38-39 0.01186172414066533 1103 40-41 -0.0037720627582231714 1103 42-43 0.008184421914926077 1103 44-45 0.0904456759848884 1103 46-47 -0.0011127285711509671 1103 48-49 -0.11035845060762739 1103 50-51 -0.11544396322815231 1103 52-53 -0.15405708302781562 1103 54-55 -0.22625296482295454 1103 56-57 -0.17367251321337918 1103 58-59 -0.03305557116362934 1103 60-61 -0.07992013250267505 1103 62-63 -0.11431394633018854 1103 64-65 -0.13612692878353627 1103 66-67 -0.08753468832755473 1103 68-69 -0.14554914078257042 1103 70-71 -0.06843149841476404 1103 72-73 -0.055848534260199756 1103 74-75 -0.20490343961701285 1103 76-77 -0.2134084717278455 1103 78-79 -0.24831779630939366 1103 80-81 -0.22725491256183616 1103 82-83 -0.2911685489416733 1103 84-85 -0.2522302641147185 1103 86-87 -0.28232452706755495 1103 88-89 -0.34108841868059514 1103 90-91 -0.3258615013535824 1103 92-93 -0.30710726636933927 1103 94-95 -0.30651439698526417 1103 96-97 -0.36514358288222937 1103 98-99 -0.3877366655408139 1103 100-101 -0.41010819899521067 1103 102-103 -0.29210577196619525 1103 104-105 -0.32063514875535404 1103 106-107 -0.4106514145155238 1103 108-109 -0.4774130443285891 1103 110-111 -0.6322169185236675 1103 112-113 -0.6511902922611448 1103 114-115 -0.7667938992347878 1103 116-117 -0.3978447224197019 1103 118-119 -0.470522579386607 1103 120-121 -0.6834352828119101 1103 122-123 -0.6615920714675276 1103 124-125 -0.9105575232581806 1104 1 -0.416415307425396 1104 2 -0.22884180808617316 1104 3 -0.323344555674808 1104 4 -0.2552052721778253 1104 5 -0.3878773532455071 1104 6 -0.03249067648339121 1104 7 -0.06651287133946937 1104 8 -0.07042922995276513 1104 9 -0.1827984660503077 1104 10-11 -0.1657483344972448 1104 12-13 0.045235863681178046 1104 14-15 -0.005809913852218074 1104 16-17 -0.03774549932867188 1104 18-19 -0.12991301972740388 1104 20-21 -0.07175018100337383 1104 22-23 -0.0346914708809436 1104 24-25 -0.10102867510765634 1104 26-27 0.11164557592190505 1104 28-29 -0.01762559200543734 1104 30-31 -0.11087842604622011 1104 32-33 -0.09996161905345247 1104 34-35 -0.05566427771976379 1104 36-37 -0.10605641402334953 1104 38-39 -0.10658525619594883 1104 40-41 -0.03153311577317908 1104 42-43 0.018154747407180594 1104 44-45 -0.05273772134307109 1104 46-47 -0.14991640778004722 1104 48-49 -0.10867225852700813 1104 50-51 -0.18639600691203384 1104 52-53 -0.17767909323380593 1104 54-55 -0.22205364567012964 1104 56-57 -0.37804123778744625 1104 58-59 -0.26307809583526875 1104 60-61 -0.20570550571202162 1104 62-63 -0.19597501990764954 1104 64-65 -0.26990614130703605 1104 66-67 -0.26927330805975913 1104 68-69 -0.3373133225228031 1104 70-71 -0.39867819463946574 1104 72-73 -0.4668600210363607 1104 74-75 -0.5629618559283145 1104 76-77 -0.525585233954132 1104 78-79 -0.5631682881294182 1104 80-81 -0.3801061784994566 1104 82-83 -0.47915548033147104 1104 84-85 -0.5495471940396008 1104 86-87 -0.5681791407949675 1104 88-89 -0.5296286559960173 1104 90-91 -0.5639368649267773 1104 92-93 -0.45984785768228775 1104 94-95 -0.5804588217930444 1104 96-97 -0.5860584662337303 1104 98-99 -0.6338660775743108 1104 100-101 -0.5651733606124161 1104 102-103 -0.47121743170925967 1104 104-105 -0.5474223725895726 1104 106-107 -0.6339950246182973 1104 108-109 -0.7221661129119141 1104 110-111 -0.7363648986277695 1104 112-113 -0.7567723386463783 1104 114-115 -0.7197729752125497 1104 116-117 -0.5952871776626658 1104 118-119 -0.5546281711766596 1104 120-121 -0.6388825305953993 1104 122-123 -0.7934035335149368 1104 124-125 -0.8385221853276263 1105 1 -0.19786119716039963 1105 2 -0.12211418267616736 1105 3 -0.06405323378803374 1105 4 -0.14536369859409604 1105 5 -0.1117072830635486 1105 6 0.07876186600928747 1105 7 0.10323074592408688 1105 8 0.06692571380481382 1105 9 -0.0068061413023343675 1105 10-11 -0.05998705263592541 1105 12-13 -0.12182161170540695 1105 14-15 -0.1085451385994105 1105 16-17 1.0105845691299464E-4 1105 18-19 -0.014217692154879558 1105 20-21 0.07057031312369588 1105 22-23 0.09352443877515526 1105 24-25 -0.11898763921040967 1105 26-27 0.025867173955454348 1105 28-29 0.1323257177873174 1105 30-31 0.06701386388564146 1105 32-33 0.038892708223265515 1105 34-35 0.09360595816597339 1105 36-37 -3.8838961542353445E-4 1105 38-39 -0.10143404168940862 1105 40-41 -0.2206808277261061 1105 42-43 -0.15470785720477664 1105 44-45 -0.08492044312564673 1105 46-47 -0.05941001653590661 1105 48-49 -0.12019161550214363 1105 50-51 -0.2727128152427767 1105 52-53 -0.2143449789374685 1105 54-55 -0.11336133113139368 1105 56-57 -0.04038904816788147 1105 58-59 -0.1683859174196911 1105 60-61 -0.10400219922810905 1105 62-63 0.008038453667442269 1105 64-65 -0.2163564026896836 1105 66-67 -0.1329961586881474 1105 68-69 -0.14702636443040973 1105 70-71 -0.20055689057959114 1105 72-73 -0.16101889770615685 1105 74-75 -0.22639007844779613 1105 76-77 -0.2491741996013559 1105 78-79 -0.3239557213439923 1105 80-81 -0.2832892353544345 1105 82-83 -0.16111222457961105 1105 84-85 -0.23107528122125132 1105 86-87 -0.20162846240425836 1105 88-89 -0.3325153177587623 1105 90-91 -0.12353544558105867 1105 92-93 -0.024159571865535412 1105 94-95 -0.14471000043636906 1105 96-97 -0.09980343687562865 1105 98-99 -0.1537007926449796 1105 100-101 -0.21487526398068013 1105 102-103 -0.06898926573324005 1105 104-105 -0.04470776264787091 1105 106-107 -0.1669769464155415 1105 108-109 -0.3528790972289535 1105 110-111 -0.3509258229040242 1105 112-113 -0.3919027158384196 1105 114-115 -0.2561823211145864 1105 116-117 -0.0445949783050672 1105 118-119 -0.0730760006666955 1105 120-121 -0.14559739445914843 1105 122-123 -0.11779150284533912 1105 124-125 -0.2816647848087861 1106 1 0.20635869461393952 1106 2 0.22295154527639838 1106 3 -0.001882839097437028 1106 4 0.05466569624478268 1106 5 -0.17415808986308434 1106 6 0.00785007710173602 1106 7 0.07319231282423289 1106 8 0.02830428579931521 1106 9 -0.07471293817890512 1106 10-11 -0.1390538292155199 1106 12-13 0.1042736596125664 1106 14-15 0.12677119556738603 1106 16-17 0.10010728017820014 1106 18-19 0.07823078367243141 1106 20-21 0.09941317603943389 1106 22-23 0.21272414286463714 1106 24-25 0.19625915449196896 1106 26-27 0.26953697615439154 1106 28-29 0.2324289288164323 1106 30-31 0.10431842297919758 1106 32-33 0.13000537676547452 1106 34-35 0.05432780326274411 1106 36-37 0.108747649251832 1106 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-0.18277425214431275 2315 36-37 -0.2041382554555824 2315 38-39 -0.19750597230847688 2315 40-41 -0.13127736129747802 2315 42-43 0.02825704919745675 2315 44-45 -0.05069168553743708 2315 46-47 -0.18994198322761235 2315 48-49 -0.3411530769413318 2315 50-51 -0.20685636496830284 2315 52-53 -0.2246100395253663 2315 54-55 -0.024099681475753698 2315 56-57 0.013135232800792096 2315 58-59 -0.05604484775342655 2315 60-61 0.061430044160101005 2315 62-63 0.12435746091076538 2315 64-65 0.20490204283618496 2315 66-67 0.06550930063589178 2315 68-69 0.16345394090430787 2315 70-71 0.1433934166137334 2315 72-73 0.055006986636271904 2315 74-75 0.06555476811260519 2315 76-77 0.10203624942182898 2315 78-79 0.01573196762505802 2315 80-81 0.23421607213993312 2315 82-83 -0.021994355011774047 2315 84-85 0.17641188524428486 2315 86-87 0.0897492479518327 2315 88-89 0.44927159975845754 2315 90-91 0.1365746440246305 2315 92-93 0.12135418835351786 2315 94-95 0.048185679485726496 2315 96-97 0.0030719685488769244 2315 98-99 0.15385770759192852 2315 100-101 0.2610414731471735 2315 102-103 0.25729919233166143 2315 104-105 0.12802519774290744 2315 106-107 0.27086431042006254 2315 108-109 0.23013567736941454 2315 110-111 0.32667857963310354 2315 112-113 0.4492378915326505 2315 114-115 0.2513267690329748 2315 116-117 0.30867701671073533 2315 118-119 0.38386287741668923 2315 120-121 0.540657111348338 2315 122-123 0.700969868019591 2315 124-125 0.7456210371787684 2316 1 -0.20538620999166568 2316 2 -0.7266172322850721 2316 3 -0.302184977603126 2316 4 0.10886543971897211 2316 5 -0.09677979822279426 2316 6 -0.5075816105862998 2316 7 -0.6995606688777798 2316 8 -0.589519696627022 2316 9 -0.5819224810849448 2316 10-11 -0.5734376837803481 2316 12-13 -0.4398707647825688 2316 14-15 -0.3976758417054924 2316 16-17 -0.34995411451212277 2316 18-19 -0.5189995221333348 2316 20-21 -0.4002351500658108 2316 22-23 -0.3344840863957259 2316 24-25 -0.5403001520490776 2316 26-27 -0.3017930718484152 2316 28-29 -0.05400603751227351 2316 30-31 -0.09457026926828149 2316 32-33 -0.31012567763587384 2316 34-35 -0.30156754661401663 2316 36-37 -0.16205858253729843 2316 38-39 -0.1607802064872743 2316 40-41 -0.1776500557722045 2316 42-43 -0.1500451641363867 2316 44-45 -0.06026731652914208 2316 46-47 -0.039746136538290955 2316 48-49 -0.20901587775608022 2316 50-51 -0.1320859005424353 2316 52-53 -0.1596350158950699 2316 54-55 -0.015598544707017936 2316 56-57 -0.12179048894011402 2316 58-59 -0.010796570656985693 2316 60-61 -0.10111501611008933 2316 62-63 -0.01745218320739994 2316 64-65 0.009318134215529739 2316 66-67 0.12461580188804078 2316 68-69 0.2015714903356276 2316 70-71 -0.023079375636417154 2316 72-73 0.20370876271724114 2316 74-75 0.21462242545190335 2316 76-77 0.31288695478054507 2316 78-79 0.15616447225866636 2316 80-81 -0.10581505032489602 2316 82-83 -0.040217416107363846 2316 84-85 -0.04970879714238308 2316 86-87 -0.09637707020436537 2316 88-89 0.09589975286796815 2316 90-91 -0.08821194373140528 2316 92-93 0.02334460606610378 2316 94-95 0.029964837197042016 2316 96-97 0.1228287429361572 2316 98-99 0.11521459676548318 2316 100-101 0.048355074877250104 2316 102-103 0.032869906447270836 2316 104-105 -0.06113977646716151 2316 106-107 0.23098554602925603 2316 108-109 0.09918916392862442 2316 110-111 0.2688043297747349 2316 112-113 0.24185976689782862 2316 114-115 -0.10381738685152087 2316 116-117 -0.05140169176614506 2316 118-119 0.08641561475876713 2316 120-121 0.07869367432769181 2316 122-123 0.27280536863420224 2316 124-125 0.3923658995313808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 11.0 11 33.0 12 85.0 13 289.0 14 784.0 15 4932.0 16 10754.0 17 14668.0 18 20989.0 19 30731.0 20 49864.0 21 85365.0 22 139108.0 23 203436.0 24 203935.0 25 144483.0 26 100093.0 27 89169.0 28 79952.0 29 82322.0 30 92380.0 31 108539.0 32 132709.0 33 169478.0 34 223949.0 35 308775.0 36 495862.0 37 1107155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.70589164007288 15.781885656502281 11.068784106519262 23.44343859690558 2 13.985696705943788 22.881038594374605 37.80637000309313 25.326894696588475 3 20.067843617615136 32.70483923616569 26.283004145543 20.94431300067618 4 12.50695540285437 18.421214581293725 35.16977337053822 33.902056645313685 5 12.870904194106261 39.74525387026839 30.06307484010722 17.32076709551813 6 27.710366370407485 35.49774081112329 20.545810596353554 16.246082222115664 7 26.00440375798973 35.00156800862392 21.091472469076383 17.90255576430997 8 29.435586128803383 28.764150220098152 21.01646960358229 20.783794047516174 9 31.96016976033187 14.153412527811959 20.094972859219492 33.79144485263667 10-11 24.05716269672867 27.253989960129243 25.814511887761864 22.87433545538022 12-13 27.65574889912461 25.094099748939126 28.21601389386415 19.034137458072117 14-15 26.001211328330236 23.113357407757373 29.445214701792455 21.440216562119936 16-17 20.40008969574603 27.182734312507623 29.307392680821998 23.109783310924353 18-19 21.110133864294607 25.024238142797984 34.58933624285068 19.276291750056735 20-21 23.400715619469324 23.977829335474958 33.50172494344637 19.11973010160935 22-23 21.63909145724726 26.092865891363243 31.397609061580457 20.870433589809043 24-25 20.5239253114108 27.162214399868713 32.236253595543864 20.077606693176627 26-27 21.50732185453219 26.280069670284163 32.473045074776316 19.739563400407327 28-29 21.000755292335057 27.023208411579574 32.43464528889772 19.54139100718765 30-31 22.037993866415032 27.079162222039876 31.672030134569624 19.210813776975467 32-33 19.43320398651128 27.787061608251186 32.30313158118092 20.476602824056613 34-35 18.906568481718917 29.057507581699916 31.800279036301642 20.235644900279524 36-37 20.69741895267281 28.97522238670144 30.67758229737495 19.6497763632508 38-39 20.462037911125133 28.344827205995955 30.520613518814848 20.67252136406406 40-41 19.662212441708817 29.142231765687526 30.47602477859223 20.719531014011423 42-43 20.346317895972902 28.818916113800896 30.234686437246026 20.600079552980173 44-45 20.687668159300866 28.384788353697456 30.167974725973817 20.759568761027865 46-47 19.553881714498342 28.183236634156074 31.08405760714843 21.178824044197157 48-49 19.513566155468506 28.72008540164642 30.322755502284576 21.4435929406005 50-51 18.40602467866575 28.394011185579586 30.26129315302931 22.938670982725345 52-53 18.70936485809893 27.563149615537206 29.659556304773023 24.06792922159084 54-55 19.179637540229926 27.342147789872236 29.702350644669373 23.77586402522846 56-57 19.733707025497598 26.127061768170634 29.77230996123497 24.3669212450968 58-59 18.957527174357015 26.33668710905082 30.489440102690917 24.21634561390125 60-61 19.75530380319002 25.90890311188106 29.982206462930733 24.353586621998186 62-63 18.861258956040395 25.955379594071747 30.661347165511643 24.52201428437622 64-65 18.571721753116204 26.107715319662244 30.645590671491757 24.674972255729795 66-67 19.23032972044629 25.629780182079624 30.50640557588929 24.633484521584798 68-69 19.485502878008575 25.434107992374734 30.444445245249778 24.635943884366913 70-71 18.754877062006376 25.752392977442497 30.364382159887338 25.128347800663796 72-73 19.385324231906228 25.667885246247618 30.095076488946564 24.85171403289959 74-75 19.76554415097817 25.17491139405697 30.098015759689385 24.96152869527548 76-77 19.014199062799054 25.302945599000925 29.738897957523786 25.943957380676242 78-79 19.365558713946974 25.438930331775346 29.357327663426148 25.83818329085153 80-81 18.794702781291384 26.121575955462184 28.369172101555613 26.71454916169082 82-83 18.797613643398055 25.706518374842663 27.46143965499453 28.034428326764747 84-85 19.581076954075897 25.521133529481 27.001084557451442 27.896704958991663 86-87 19.616755331554625 25.07429115121583 26.88922184931685 28.41973166791269 88-89 19.804539612269323 25.197348719896024 26.4212529469816 28.576858720853053 90-91 20.443485723980064 25.494357337064717 25.714160046789342 28.347996892165877 92-93 20.004901413583074 26.462090388738552 24.958447047411553 28.57456115026682 94-95 19.722771210925554 26.515588109535056 25.4176733833823 28.34396729615709 96-97 20.23781164972713 26.604118977878898 24.90265653739079 28.255412835003185 98-99 20.678507973766177 27.091406825542823 24.36857203005961 27.86151317063139 100-101 20.499808838850512 27.12916467834284 24.465229569027123 27.90579691377953 102-103 20.24454267611763 28.185256308510247 24.603157915520363 26.96704309985176 104-105 20.234580757059693 28.39653361115591 24.890002207776657 26.478883424007737 106-107 19.49509096629589 28.696186085058123 25.322211028062352 26.486511920583634 108-109 19.3246485570859 29.41125186577641 25.315531285682454 25.94856829145524 110-111 19.176168525412894 29.7373797101479 25.61241442301257 25.474037341426637 112-113 18.453817901968712 30.028420226858348 25.5968530198546 25.92090885131834 114-115 18.625546175927397 29.57365596354898 26.054142333324688 25.746655527198936 116-117 18.515413481941366 29.671313877751494 26.50619396301278 25.307078677294363 118-119 18.24485472259878 30.146757026744826 26.622731952323836 24.98565629833256 120-121 18.158512381224075 30.56228126812267 26.870606505565398 24.408599845087856 122-123 17.65934322128551 31.238134534416716 26.775024867088813 24.32749737720896 124-125 17.07376551045056 32.093048600052825 27.142499980768196 23.69068590872842 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 53.0 1 38.5 2 42.5 3 91.5 4 158.0 5 326.0 6 807.5 7 1618.0 8 2975.0 9 4029.0 10 4293.0 11 4713.0 12 5409.0 13 6235.0 14 7103.5 15 8188.5 16 9501.5 17 10897.0 18 12483.5 19 14554.0 20 16829.0 21 19361.5 22 22519.0 23 26143.5 24 30342.0 25 35567.0 26 41879.5 27 49113.0 28 56749.0 29 64813.0 30 73193.5 31 80325.0 32 86246.0 33 89187.0 34 87582.5 35 83487.0 36 79744.0 37 76131.0 38 71214.0 39 66666.5 40 63076.5 41 60727.5 42 60658.0 43 65059.0 44 72974.5 45 82147.0 46 96542.5 47 109610.5 48 136377.0 49 171036.0 50 217440.5 51 231195.5 52 192703.0 53 177383.5 54 175587.0 55 158395.5 56 130943.0 57 102495.0 58 80833.0 59 64744.5 60 49026.0 61 38062.5 62 30039.0 63 21273.5 64 13905.0 65 9877.0 66 7494.5 67 5565.5 68 4336.0 69 3526.5 70 3025.0 71 2578.5 72 2155.5 73 2159.0 74 1798.0 75 1316.0 76 1024.0 77 712.0 78 490.0 79 306.0 80 214.5 81 154.5 82 117.0 83 85.5 84 52.0 85 23.5 86 12.5 87 5.0 88 2.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1158505799324128 2 0.02279574270914453 3 0.0 4 1.2821002648562727E-4 5 0.021334148407208375 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.2821002648562728E-5 18-19 1.5385203178275273E-4 20-21 3.3334606886263086E-4 22-23 3.846300794568818E-5 24-25 0.0015000573098818391 26-27 0.001371847283396212 28-29 9.359331933450791E-4 30-31 2.8206205826838E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 5.000191032939463E-4 40-41 8.461861748051401E-4 42-43 0.33675645556714856 44-45 0.3215507464259532 46-47 0.3192814289571576 48-49 0.3283843408376371 50-51 0.33261527171166283 52-53 0.3366538875459601 54-55 0.3366795295512572 56-57 0.3392309090783212 58-59 0.33941040311540105 60-61 0.356154632574424 62-63 0.3499364462898711 64-65 0.35677004070155505 66-67 0.36945001232098357 68-69 0.38137354478414687 70-71 0.3670653058283509 72-73 0.3706039025593542 74-75 0.3798222034636708 76-77 0.3760400076823448 78-79 0.4513121142320565 80-81 0.6165235543614358 82-83 0.5849197828327287 84-85 0.5464439538843919 86-87 0.5002755233469176 88-89 0.3288074339250397 90-91 0.017244248562316867 92-93 0.07733628797613037 94-95 8.97470185399391E-5 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.17562209428001224 114-115 0.1586727287786123 116-117 0.1522750484569795 118-119 0.13550517699265946 120-121 0.13809501952766914 122-123 0.13192811725371045 124-125 0.0015641623231246525 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3899851.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.654019971552074 #Duplication Level Percentage of deduplicated Percentage of total 1 70.70656962586148 23.79560306308679 2 15.35097812438905 10.33244248762096 3 6.068725433977257 6.1271102087081 4 2.57661965764725 3.4685443767021686 5 1.3744248006295035 2.312745984489089 6 0.7882224869322617 1.591611319034687 7 0.5178206188205541 1.2198721813227866 8 0.3515090427091151 0.9463753874810968 9 0.25810824195053567 0.7817741936482969 >10 1.6235023507290023 10.448392770036904 >50 0.19138179663954882 4.488790183366922 >100 0.15442494640964172 10.494583659675204 >500 0.02030092558867651 4.643027349903236 >1k 0.015538016123511734 10.558397757694193 >5k 0.0010931267624581119 2.6336870646189623 >10k+ 7.808048303272228E-4 6.1570420126107095 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 69632 1.7855041128494398 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 27355 0.7014370549028668 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26720 0.6851543815391922 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 26532 0.6803336845433325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25961 0.6656920995186739 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13722 0.3518595966871555 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 11835 0.30347313269147974 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 11032 0.282882602437888 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 11011 0.2823441203266484 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 10511 0.2695231176780857 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 9570 0.2453939906934906 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 8691 0.22285466803731732 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 8596 0.22041867753409042 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 8415 0.2157774745753107 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7394 0.18959698716694562 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 7335 0.1880841088544152 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 7230 0.18539169829821706 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 7167 0.18377625196449812 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 6292 0.16133949732951336 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 6266 0.1606728051917881 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 6184 0.1585701607574238 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 5936 0.1522109434437367 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5921 0.15182631336427982 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5230 0.1341076877039661 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4957 0.12710742025785088 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 4852 0.1244150097016527 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 4782 0.12262006933085393 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 4708 0.12072256093886664 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 4570 0.11718396420786334 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 4479 0.11485054172582491 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 4261 0.10926058457105157 No Hit CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA 4230 0.10846568240684067 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 4110 0.10538864177118562 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 4003 0.1026449472043932 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG 3969 0.10177311902429094 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 3963 0.10161926699250817 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.5642005297125453E-5 0.0 5 0.0 0.0 0.0 2.5642005297125453E-5 0.0 6 0.0 0.0 0.0 2.5642005297125453E-5 0.0 7 0.0 0.0 0.0 2.5642005297125453E-5 0.0 8 0.0 0.0 0.0 2.5642005297125453E-5 0.0 9 0.0 0.0 0.0 5.1284010594250905E-5 0.0 10-11 0.0 0.0 0.0 1.0256802118850181E-4 0.0 12-13 5.1284010594250905E-5 0.0 0.0 1.923150397284409E-4 0.0 14-15 5.1284010594250905E-5 0.0 0.0 3.589880741597563E-4 0.0 16-17 5.1284010594250905E-5 0.0 0.0 0.0011410692357220827 0.0 18-19 5.1284010594250905E-5 0.0 0.0 0.0022308544608499146 0.0 20-21 7.692601589137636E-5 0.0 0.0 0.004897623011750962 0.0 22-23 1.0256802118850181E-4 0.0 0.0 0.018680200858955893 0.0 24-25 1.0256802118850181E-4 0.0 0.0 0.049360860196966505 2.5642005297125453E-5 26-27 1.2821002648562727E-4 0.0 0.0 0.08566993969769614 2.5642005297125453E-5 28-29 1.2821002648562727E-4 0.0 0.0 0.17771191771172795 2.5642005297125453E-5 30-31 1.2821002648562727E-4 0.0 0.0 0.42095197996026 2.5642005297125453E-5 32-33 1.2821002648562727E-4 0.0 0.0 0.8170183937796598 2.5642005297125453E-5 34-35 1.5385203178275273E-4 0.0 0.0 1.1402230495472776 2.5642005297125453E-5 36-37 1.5385203178275273E-4 0.0 0.0 1.544187200998192 2.5642005297125453E-5 38-39 1.5385203178275273E-4 0.0 0.0 2.116427012211492 2.5642005297125453E-5 40-41 1.5385203178275273E-4 0.0 0.0 2.9335736160176378 2.5642005297125453E-5 42-43 1.5385203178275273E-4 0.0 0.0 3.647729105547879 2.5642005297125453E-5 44-45 1.5385203178275273E-4 0.0 0.0 4.299100145108108 3.8463007945688176E-5 46-47 1.923150397284409E-4 0.0 0.0 4.951496864880222 5.1284010594250905E-5 48-49 2.3077804767412908E-4 0.0 0.0 5.551840313899172 5.1284010594250905E-5 50-51 2.3077804767412908E-4 0.0 0.0 6.17013316662611 5.1284010594250905E-5 52-53 2.3077804767412908E-4 0.0 0.0 6.8904427374276604 7.692601589137635E-5 54-55 2.3077804767412908E-4 0.0 0.0 7.545595972769216 1.0256802118850181E-4 56-57 2.3077804767412908E-4 0.0 0.0 8.162568262223353 1.0256802118850181E-4 58-59 2.3077804767412908E-4 0.0 0.0 8.851658691575652 1.0256802118850181E-4 60-61 2.3077804767412908E-4 0.0 0.0 9.50813249019001 1.0256802118850181E-4 62-63 2.3077804767412908E-4 1.2821002648562726E-5 0.0 10.163247262523619 1.0256802118850181E-4 64-65 2.3077804767412908E-4 2.5642005297125453E-5 0.0 10.831375352545521 1.0256802118850181E-4 66-67 2.3077804767412908E-4 2.5642005297125453E-5 0.0 11.466284224704996 1.0256802118850181E-4 68-69 2.3077804767412908E-4 2.5642005297125453E-5 0.0 12.16605454926355 1.0256802118850181E-4 70-71 2.3077804767412908E-4 2.5642005297125453E-5 0.0 12.967854412899364 1.0256802118850181E-4 72-73 2.3077804767412908E-4 2.5642005297125453E-5 0.0 13.69330520576299 1.0256802118850181E-4 74-75 2.3077804767412908E-4 2.5642005297125453E-5 0.0 14.36535652259535 1.0256802118850181E-4 76-77 2.3077804767412908E-4 2.5642005297125453E-5 0.0 15.05366487078609 1.0256802118850181E-4 78-79 2.3077804767412908E-4 2.5642005297125453E-5 0.0 15.722536578961606 1.0256802118850181E-4 80-81 2.3077804767412908E-4 2.5642005297125453E-5 0.0 16.396805929252167 1.0256802118850181E-4 82-83 2.3077804767412908E-4 2.5642005297125453E-5 0.0 17.15645033617951 1.0256802118850181E-4 84-85 2.3077804767412908E-4 2.5642005297125453E-5 0.0 17.83634810663279 1.0256802118850181E-4 86-87 2.3077804767412908E-4 2.5642005297125453E-5 0.0 18.484731852575905 1.0256802118850181E-4 88-89 2.3077804767412908E-4 2.5642005297125453E-5 0.0 19.13982098290422 1.0256802118850181E-4 90-91 2.3077804767412908E-4 2.5642005297125453E-5 0.0 19.795781941412635 1.0256802118850181E-4 92-93 2.3077804767412908E-4 2.5642005297125453E-5 0.0 20.424216207234583 1.0256802118850181E-4 94-95 2.3077804767412908E-4 2.5642005297125453E-5 0.0 21.06786900320038 1.0256802118850181E-4 96-97 2.3077804767412908E-4 2.5642005297125453E-5 0.0 21.69502116875747 1.0256802118850181E-4 98-99 2.3077804767412908E-4 2.5642005297125453E-5 0.0 22.33521229400816 1.0256802118850181E-4 100-101 2.3077804767412908E-4 2.5642005297125453E-5 0.0 23.047303602111977 1.0256802118850181E-4 102-103 2.3077804767412908E-4 2.5642005297125453E-5 0.0 23.674340378645237 1.2821002648562727E-4 104-105 2.3077804767412908E-4 2.5642005297125453E-5 0.0 24.271914491092097 1.2821002648562727E-4 106-107 2.3077804767412908E-4 2.5642005297125453E-5 0.0 24.87007836966079 1.2821002648562727E-4 108-109 2.3077804767412908E-4 2.5642005297125453E-5 0.0 25.483755661434245 1.2821002648562727E-4 110-111 2.3077804767412908E-4 2.5642005297125453E-5 0.0 26.07475259952239 1.5385203178275273E-4 112-113 2.3077804767412908E-4 2.5642005297125453E-5 0.0 26.70802038334285 1.794940370798782E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 790 0.0 63.985916 1 GACCGTT 810 0.0 46.90673 7 ATAACGC 890 0.0 42.70463 3 TAACGCA 960 0.0 42.065174 4 ATAGTAC 1255 0.0 41.641273 4 TACAACG 675 0.0 41.359364 2 TAGTACT 1310 0.0 39.89297 5 GTATAAC 1010 0.0 39.44991 1 GTACTGG 3405 0.0 37.89965 1 GCATAGT 1375 0.0 37.58342 2 TCACGCA 650 0.0 37.458942 4 TTAGGAC 1095 0.0 36.33672 3 TACTGGT 3180 0.0 35.676807 2 AGATTAC 1620 0.0 35.199455 2 CATGGGT 4925 0.0 34.968483 4 GTACAAC 830 0.0 34.391865 1 TAGGACC 1525 0.0 32.71105 4 GGTTCAC 3450 0.0 32.694504 6 ACTGGTT 3510 0.0 32.65391 3 CATGGGG 14395 0.0 32.096153 4 >>END_MODULE