##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557047.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2518701 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.637874841039093 33.0 32.0 34.0 27.0 34.0 2 31.89152384502964 33.0 32.0 34.0 27.0 34.0 3 32.05730731833592 33.0 32.0 34.0 28.0 34.0 4 31.93424547018483 33.0 32.0 34.0 28.0 34.0 5 32.075279677897456 33.0 32.0 34.0 28.0 34.0 6 35.474926162335265 38.0 36.0 38.0 29.0 38.0 7 35.81569983892491 38.0 36.0 38.0 31.0 38.0 8 36.02237859912709 38.0 37.0 38.0 31.0 38.0 9 36.12293519556311 38.0 37.0 38.0 33.0 38.0 10-11 36.105898040299344 38.0 37.0 38.0 32.5 38.0 12-13 36.0656673817178 38.0 37.0 38.0 32.0 38.0 14-15 36.06716755978578 38.0 37.0 38.0 31.0 38.0 16-17 36.03819984984324 38.0 37.0 38.0 31.0 38.0 18-19 36.01547245981162 38.0 37.0 38.0 31.0 38.0 20-21 35.984737966118246 38.0 37.0 38.0 31.0 38.0 22-23 35.873796254497854 38.0 37.0 38.0 31.0 38.0 24-25 35.80302723507078 38.0 37.0 38.0 30.0 38.0 26-27 35.564374254824216 38.0 37.0 38.0 29.0 38.0 28-29 35.53806287447378 38.0 37.0 38.0 29.0 38.0 30-31 35.4370014543211 38.0 37.0 38.0 29.0 38.0 32-33 35.349083515669385 38.0 36.5 38.0 27.5 38.0 34-35 35.08980819875007 38.0 36.0 38.0 27.0 38.0 36-37 34.88770898173304 38.0 36.0 38.0 25.5 38.0 38-39 34.64456757669926 38.0 35.5 38.0 24.5 38.0 40-41 34.29400671219013 38.0 35.0 38.0 20.0 38.0 42-43 33.70422491593881 38.0 34.0 38.0 15.5 38.0 44-45 33.24348701969785 38.0 33.5 38.0 15.0 38.0 46-47 32.57202760470576 38.0 30.0 38.0 15.0 38.0 48-49 31.973460327367164 38.0 27.0 38.0 15.0 38.0 50-51 31.496156947569403 38.0 25.5 38.0 15.0 38.0 52-53 31.149042700979592 38.0 24.5 38.0 15.0 38.0 54-55 30.86544889607778 38.0 24.0 38.0 15.0 38.0 56-57 30.575222902599393 38.0 20.0 38.0 15.0 38.0 58-59 30.391836704714056 37.5 16.0 38.0 15.0 38.0 60-61 30.21935374623665 37.0 16.0 38.0 15.0 38.0 62-63 30.05928730722702 37.0 15.5 38.0 15.0 38.0 64-65 29.8228414170638 37.0 15.0 38.0 15.0 38.0 66-67 29.674537787534128 37.0 15.0 38.0 15.0 38.0 68-69 29.479412006427122 37.0 15.0 38.0 14.5 38.0 70-71 29.293199351570514 36.0 15.0 38.0 14.0 38.0 72-73 29.056702840075104 36.0 15.0 38.0 14.0 38.0 74-75 28.907467182488116 36.0 15.0 38.0 14.0 38.0 76-77 28.761676554700223 36.0 14.5 38.0 14.0 38.0 78-79 28.580561567252325 36.0 14.0 38.0 14.0 38.0 80-81 28.405608486279235 35.5 14.0 38.0 14.0 38.0 82-83 28.286049832830493 35.0 14.0 38.0 14.0 38.0 84-85 28.178254981436858 35.0 14.0 38.0 13.0 38.0 86-87 28.13459120395791 35.0 14.0 38.0 13.0 38.0 88-89 28.056252409476155 34.5 14.0 38.0 13.0 38.0 90-91 27.956415628532326 34.0 14.0 38.0 13.0 38.0 92-93 27.887050308869533 34.0 14.0 38.0 13.0 38.0 94-95 27.84836786899279 34.0 14.0 38.0 13.0 38.0 96-97 27.79147862330622 34.0 14.0 38.0 13.0 38.0 98-99 27.68773149333724 34.0 14.0 38.0 13.0 38.0 100-101 27.597310875725224 34.0 14.0 38.0 13.0 38.0 102-103 27.46177017438751 34.0 14.0 38.0 13.0 38.0 104-105 27.381792439833074 34.0 14.0 38.0 13.0 38.0 106-107 27.21050017449471 33.0 14.0 38.0 13.0 38.0 108-109 27.044223391343394 32.5 14.0 38.0 13.0 38.0 110-111 26.853284689210827 31.0 14.0 38.0 13.0 38.0 112-113 26.68278509438 31.0 14.0 38.0 13.0 38.0 114-115 26.53792331840897 29.0 14.0 38.0 13.0 38.0 116-117 26.380757183961094 28.0 14.0 38.0 13.0 38.0 118-119 26.27636309351527 27.5 14.0 38.0 13.0 38.0 120-121 26.077687665189316 25.0 14.0 38.0 13.0 38.0 122-123 26.028390031210535 25.0 14.0 38.0 13.0 38.0 124-125 25.085927626979146 24.0 14.0 36.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.44017845215718765 1101 2 -0.3197793065483623 1101 3 -0.5358704218426809 1101 4 -0.4341964363753732 1101 5 -0.6097119571802132 1101 6 0.21929341967781824 1101 7 0.1818099357686549 1101 8 -0.0569735435019183 1101 9 -0.007668867035569349 1101 10-11 -0.04189784560781362 1101 12-13 -0.09364732832281675 1101 14-15 -0.04845247416374576 1101 16-17 -0.006590408385299895 1101 18-19 -0.054310983773540045 1101 20-21 -0.055268363523943265 1101 22-23 0.07481506090454104 1101 24-25 -0.01194171758042728 1101 26-27 0.11320825714972926 1101 28-29 -0.09364606614783355 1101 30-31 -0.06776458307489008 1101 32-33 0.03397419670098856 1101 34-35 0.12757756568343126 1101 36-37 0.0635976557665856 1101 38-39 -0.04581064946925295 1101 40-41 -0.044587583011207244 1101 42-43 -0.03382469701501378 1101 44-45 -0.13244812439728548 1101 46-47 -0.02874742917698825 1101 48-49 -0.20581441997962102 1101 50-51 -0.017776015055535055 1101 52-53 -0.01212832991645385 1101 54-55 -0.10841304641579796 1101 56-57 -0.2711844649659767 1101 58-59 -0.14449165783403828 1101 60-61 -0.22280606339110065 1101 62-63 -0.18766961253551173 1101 64-65 -0.2058500156323504 1101 66-67 -0.12759486915474483 1101 68-69 -0.4091661064155616 1101 70-71 -0.35492585406394284 1101 72-73 -0.30356163387533286 1101 74-75 -0.5253083758147703 1101 76-77 -0.3473677321031907 1101 78-79 -0.45648217923747225 1101 80-81 -0.3650289996061389 1101 82-83 -0.3096664212394593 1101 84-85 -0.3706581158832023 1101 86-87 -0.17867099533623687 1101 88-89 -0.5077815638310987 1101 90-91 -0.37341860315599007 1101 92-93 -0.391470368931774 1101 94-95 -0.3875712867511645 1101 96-97 -0.30696845463596034 1101 98-99 -0.4120767181158804 1101 100-101 -0.1723326758017798 1101 102-103 -0.24624306424110287 1101 104-105 -0.33251761625204423 1101 106-107 -0.26435900148382885 1101 108-109 -0.5072453773423042 1101 110-111 -0.5700710515630405 1101 112-113 -0.534506676925993 1101 114-115 -0.4690297773324055 1101 116-117 -0.5410361492372004 1101 118-119 -0.6629127936077062 1101 120-121 -0.7626447929804101 1101 122-123 -0.6105755342732095 1101 124-125 -0.646414625005832 1102 1 -0.5393091299186139 1102 2 -0.771211460460826 1102 3 -0.5940432583019088 1102 4 -0.375965268041103 1102 5 -0.41092260067299335 1102 6 -0.09532601980073707 1102 7 0.17081854388678863 1102 8 0.11037049984735603 1102 9 0.08080392114472801 1102 10-11 0.0128033577840867 1102 12-13 0.09446676315114644 1102 14-15 0.13021859097451483 1102 16-17 0.1502932581755303 1102 18-19 0.13905678841513236 1102 20-21 0.07732371174509467 1102 22-23 0.11606970329734168 1102 24-25 0.025502381778082395 1102 26-27 0.18458430962784433 1102 28-29 -0.008880720742467929 1102 30-31 -0.09023751415941206 1102 32-33 0.0030599872867114186 1102 34-35 0.053492449726221025 1102 36-37 0.03561227384084731 1102 38-39 0.14717034160880615 1102 40-41 0.18280504777586515 1102 42-43 0.12570154532538425 1102 44-45 0.2947683228511053 1102 46-47 0.31808326175787016 1102 48-49 0.21132834294066427 1102 50-51 0.27915095893323993 1102 52-53 0.22564648812444332 1102 54-55 0.2778145319372989 1102 56-57 0.21632746071106013 1102 58-59 0.12757166469637582 1102 60-61 0.16212305451569975 1102 62-63 0.20942542462756464 1102 64-65 0.19556113703209377 1102 66-67 0.20187162822537275 1102 68-69 0.07346039175176955 1102 70-71 0.040496884481065365 1102 72-73 0.07430454003769427 1102 74-75 0.11187817679091694 1102 76-77 -0.1776264082604051 1102 78-79 -0.008837076038812341 1102 80-81 0.07236325707680535 1102 82-83 0.17888971592094194 1102 84-85 0.039371083741414736 1102 86-87 0.026356503089775885 1102 88-89 0.04952382977519321 1102 90-91 -0.09918512211358532 1102 92-93 0.040365289077485045 1102 94-95 -0.07406822007251179 1102 96-97 -0.2059989044066306 1102 98-99 -0.19296765683531802 1102 100-101 -0.1527101167203675 1102 102-103 0.030476968112697733 1102 104-105 0.04913250561547855 1102 106-107 0.005849580181060077 1102 108-109 -0.23227929050156604 1102 110-111 -0.19769667297924087 1102 112-113 -0.2977438016716327 1102 114-115 -0.30723532928580255 1102 116-117 -0.33019416851366756 1102 118-119 -0.23659330975083037 1102 120-121 -0.40817049880939393 1102 122-123 -0.5523023457128495 1102 124-125 -0.46686530168217644 1103 1 -0.40810519624424657 1103 2 -0.2806868203237656 1103 3 -0.31107897626193903 1103 4 -0.3605460717719602 1103 5 -0.3450449383623315 1103 6 -0.1773055186867296 1103 7 0.068337930997167 1103 8 -0.03945216987952449 1103 9 -0.11940126595204248 1103 10-11 -0.1289244044717961 1103 12-13 0.009465297144274132 1103 14-15 -0.052558261234935344 1103 16-17 -0.07448976293336074 1103 18-19 -0.12621989187740468 1103 20-21 -0.10458592513587206 1103 22-23 -0.10198943647890957 1103 24-25 -0.08281029908254567 1103 26-27 -0.061807724487643156 1103 28-29 -0.08501809698697826 1103 30-31 -0.13952173832757353 1103 32-33 -0.08379315401676735 1103 34-35 0.02072320136131367 1103 36-37 0.0031405359934026933 1103 38-39 -0.032171134930322864 1103 40-41 -0.17378213492522576 1103 42-43 -0.13683236449966074 1103 44-45 -0.045252892503597764 1103 46-47 -0.1508738408882806 1103 48-49 -0.209462040341581 1103 50-51 -0.19623287362346176 1103 52-53 -0.39316757785681844 1103 54-55 -0.20521946846955785 1103 56-57 -0.3290879596702254 1103 58-59 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112-113 -0.1490278640746503 1104 114-115 -0.12354127988016828 1104 116-117 -0.11307891571222584 1104 118-119 -0.028173265256103974 1104 120-121 -0.1353587252236359 1104 122-123 -0.25580095308530204 1104 124-125 -0.5710314006937338 1105 1 -0.1194992047768153 1105 2 0.03982569659156354 1105 3 -0.09341561355435957 1105 4 -0.10765266229004311 1105 5 -0.1763968228751125 1105 6 -0.05198021067585756 1105 7 -0.07478043392917755 1105 8 0.013419755459992189 1105 9 -0.16593331009955392 1105 10-11 -0.33303010645241926 1105 12-13 -0.18685600678529113 1105 14-15 -0.07713246509474203 1105 16-17 0.008155803285326613 1105 18-19 -0.00650371249923154 1105 20-21 0.06635028583703928 1105 22-23 0.14521522262848663 1105 24-25 -0.17497332322612635 1105 26-27 -0.011594290171977661 1105 28-29 -6.316631589911026E-4 1105 30-31 -0.1295341860886765 1105 32-33 0.0011634160982367803 1105 34-35 -0.06762175434393924 1105 36-37 -0.08974474657970433 1105 38-39 -0.12807449335480214 1105 40-41 -0.06655850387864604 1105 42-43 -0.10536890658919162 1105 44-45 0.03255428011119221 1105 46-47 -0.004211021461813402 1105 48-49 -0.12094162508673989 1105 50-51 -0.09679063765275941 1105 52-53 -0.1554427965849534 1105 54-55 -0.14880497621431488 1105 56-57 -0.08693396161203637 1105 58-59 -0.21770656870116056 1105 60-61 -0.2952571742604633 1105 62-63 -0.14285848760850328 1105 64-65 -0.13006470384097213 1105 66-67 -0.13147893829478718 1105 68-69 -0.2124285109850419 1105 70-71 -0.3292254880160783 1105 72-73 -0.29896671814835685 1105 74-75 -0.4014668693554668 1105 76-77 -0.43438204765536526 1105 78-79 -0.3082802398378668 1105 80-81 -0.43661675806538014 1105 82-83 -0.23682023556260035 1105 84-85 -0.35549505496255307 1105 86-87 -0.25837274139235333 1105 88-89 -0.24640937404743113 1105 90-91 -0.0548226700457235 1105 92-93 -0.014828177755216387 1105 94-95 -0.07922281297860678 1105 96-97 -0.20164284961994383 1105 98-99 -0.25615798406332857 1105 100-101 -0.15218250501220254 1105 102-103 -0.037275349046066 1105 104-105 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62-63 0.2070738167510946 2314 64-65 0.2304235566701074 2314 66-67 0.199647517161484 2314 68-69 0.22017675193265518 2314 70-71 0.2941947695620293 2314 72-73 0.3625856569798067 2314 74-75 0.2586132396877545 2314 76-77 0.3038446916662174 2314 78-79 0.22024715940211692 2314 80-81 0.11836199006728165 2314 82-83 0.3017413309601551 2314 84-85 0.13840260139916438 2314 86-87 0.19273427248448982 2314 88-89 0.18356412577153947 2314 90-91 0.12958116715614665 2314 92-93 0.15711798823753398 2314 94-95 0.09229633804199722 2314 96-97 0.149439367876262 2314 98-99 0.19847087648973627 2314 100-101 0.17491856990329424 2314 102-103 0.09076433733295985 2314 104-105 0.005108301913455904 2314 106-107 0.31959472344386697 2314 108-109 0.33830969383749476 2314 110-111 0.2867577091069009 2314 112-113 0.3240468520638977 2314 114-115 0.29596649609927184 2314 116-117 0.3138510458990389 2314 118-119 0.2365558774370946 2314 120-121 0.33951474164386397 2314 122-123 0.341859860157097 2314 124-125 0.45120693377785415 2315 1 0.6087288357989138 2315 2 0.265808447374809 2315 3 0.11677230464235322 2315 4 0.17296475870822547 2315 5 -0.08311621249936252 2315 6 -0.1521725153050113 2315 7 -0.32950228142590987 2315 8 -0.5004204803781391 2315 9 -0.4744863632648304 2315 10-11 -0.3802534824615762 2315 12-13 -0.3086075349914097 2315 14-15 -0.22054659822908462 2315 16-17 -0.24175214902263065 2315 18-19 -0.23982332291542008 2315 20-21 -0.17489706606879452 2315 22-23 -0.2540054198245798 2315 24-25 -0.18847247718377957 2315 26-27 -0.11558735274726217 2315 28-29 -0.10470957502111133 2315 30-31 -0.17844780890204248 2315 32-33 -0.19164035353809794 2315 34-35 -0.31714099277105134 2315 36-37 -0.05871870121810474 2315 38-39 -0.23422324855143728 2315 40-41 -0.07802280652778393 2315 42-43 0.17590862348176728 2315 44-45 -0.06837364026647919 2315 46-47 0.054726304015147775 2315 48-49 -0.004781879303781267 2315 50-51 0.06412925114259949 2315 52-53 0.05775068551950824 2315 54-55 0.25619736403316296 2315 56-57 0.2355988539262306 2315 58-59 0.244720819300845 2315 60-61 0.21136244853085273 2315 62-63 0.2275489298732687 2315 64-65 0.31805807460865765 2315 66-67 0.34316184596992727 2315 68-69 0.41161547954124345 2315 70-71 0.2727858689231226 2315 72-73 0.38590195843556785 2315 74-75 0.4710452392810254 2315 76-77 0.562491547124047 2315 78-79 0.58364811183149 2315 80-81 0.5371724701798115 2315 82-83 0.36554859111572924 2315 84-85 0.34228434424323595 2315 86-87 0.2923665195060323 2315 88-89 0.615156563956841 2315 90-91 0.3798747763889523 2315 92-93 0.46126622488742086 2315 94-95 0.3730549768475626 2315 96-97 0.36305978579278175 2315 98-99 0.48746116577745013 2315 100-101 0.3901773527595651 2315 102-103 0.4189995467148968 2315 104-105 0.23489036166907695 2315 106-107 0.5121244233099809 2315 108-109 0.41429517013692063 2315 110-111 0.3844554398846043 2315 112-113 0.3757216533586565 2315 114-115 0.3556428904253721 2315 116-117 0.4420396506408508 2315 118-119 0.3583397335897551 2315 120-121 0.6677088542067793 2315 122-123 0.6204183362700952 2315 124-125 0.7110875411634154 2316 1 -0.2294512099847239 2316 2 -0.5773645659240927 2316 3 -0.18296488724751825 2316 4 0.08299711522524333 2316 5 0.07509713691558062 2316 6 -0.6846789920849261 2316 7 -0.7195561638535892 2316 8 -0.616926653050605 2316 9 -0.7353116541803288 2316 10-11 -0.8326645326438111 2316 12-13 -0.5373639062679416 2316 14-15 -0.26159828489790726 2316 16-17 -0.3062656930792329 2316 18-19 -0.3514869710579447 2316 20-21 -0.27060570407333273 2316 22-23 -0.18265026356038305 2316 24-25 -0.11215205810825069 2316 26-27 -0.05537665525233848 2316 28-29 0.05014796159259305 2316 30-31 -0.06482501939916574 2316 32-33 -0.1964586364520855 2316 34-35 -0.17639167395240207 2316 36-37 -0.14528333725883869 2316 38-39 -0.20356453055762103 2316 40-41 -0.009374657556371346 2316 42-43 0.178792812020653 2316 44-45 0.15520533392497526 2316 46-47 -0.14059564397345525 2316 48-49 -0.34114831395965695 2316 50-51 -0.3954751965576868 2316 52-53 -0.2267305567851885 2316 54-55 -0.1452617557432916 2316 56-57 -0.12586514316670971 2316 58-59 -0.020581519556035488 2316 60-61 -0.008781947741770324 2316 62-63 -0.22426606648968672 2316 64-65 -0.09910518276868885 2316 66-67 -0.0698384574932227 2316 68-69 -0.012455994084845656 2316 70-71 -0.10248843986062184 2316 72-73 0.01959736969901016 2316 74-75 -0.1017830985076138 2316 76-77 -0.09453798995612317 2316 78-79 -0.08961001933804269 2316 80-81 -0.23068176184257183 2316 82-83 -0.18726277593100704 2316 84-85 -0.024800405734694664 2316 86-87 -0.13575942478947312 2316 88-89 0.07762305254024682 2316 90-91 -0.24879045929754184 2316 92-93 -0.20625308625766436 2316 94-95 -0.2491138279135221 2316 96-97 -0.24618872089146038 2316 98-99 -0.13220833643345387 2316 100-101 -0.12509542904398785 2316 102-103 -0.2515550402498441 2316 104-105 -0.39151267218929675 2316 106-107 -0.2081782280301816 2316 108-109 -0.21362832042383317 2316 110-111 -0.2441514065276067 2316 112-113 -0.22716161705922744 2316 114-115 -0.26098610106542 2316 116-117 -0.34739098869995644 2316 118-119 -0.3134965986167053 2316 120-121 -0.11544304553054374 2316 122-123 0.04150148710674628 2316 124-125 -0.12351400720738326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 12.0 11 26.0 12 71.0 13 215.0 14 500.0 15 3526.0 16 7752.0 17 11182.0 18 16525.0 19 25645.0 20 43276.0 21 75250.0 22 126156.0 23 190526.0 24 192714.0 25 127749.0 26 84890.0 27 68522.0 28 54476.0 29 50068.0 30 54408.0 31 61795.0 32 74971.0 33 96010.0 34 129990.0 35 171446.0 36 267927.0 37 583070.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.35297461257434 15.71675981895028 11.492690318244133 20.43757525023124 2 13.920670486018171 23.66235990173364 38.90976921066824 23.50720040157995 3 20.032548524020914 35.8379974439205 26.010034537644604 18.119419494413986 4 12.325901974672608 18.08181694019445 34.81571416320191 34.77656692193103 5 12.039444344945816 41.01309635804575 28.39736301719376 18.550096279814674 6 26.46038573058096 35.795316712861116 20.756890158855697 16.987407397702228 7 25.182385682143295 36.74270983336251 20.7998091079489 17.27509537654529 8 30.52196350420316 26.908354743179125 20.738467964240296 21.831213788377422 9 33.910615035290014 13.50104676974361 20.482860013951637 32.10547818101473 10-11 23.272115268942205 28.34842246062554 25.17680740985135 23.20265486058091 12-13 27.913217964339555 26.30796589194192 28.629440334521643 17.149375809196883 14-15 26.171268443534977 23.108757252250268 31.560038289578635 19.159936014636116 16-17 18.764914685171977 27.899228193268712 30.367862316301604 22.96799480525771 18-19 19.28077522593022 24.909199117956977 38.09039432278106 17.719631333331744 20-21 22.865216774179462 23.727330747997225 36.40779888771654 16.99965359010677 22-23 20.572672296686324 26.35474126439408 34.42499415869414 18.64759228022546 24-25 18.665482444011634 27.736061545168372 35.7511433115209 17.847312699299096 26-27 19.71124011232926 27.07997921122562 35.8280590486952 17.380721627749917 28-29 19.25853562861152 28.106649599512124 35.63357443865393 17.001240333222427 30-31 20.053718347912657 28.580563129480872 34.4358829035303 16.929835619076165 32-33 17.613007657518697 29.259030746404598 35.071689732127794 18.05627186394892 34-35 16.933034131482856 30.78475769851205 34.029982121736566 18.252226048268533 36-37 18.20482065953839 31.283070122257467 32.891855762156766 17.620253456047383 38-39 18.075875599543174 30.574265138384522 32.78051255643562 18.569346705636683 40-41 17.47410744843128 31.65263681979197 32.32356418724426 18.54969154453249 42-43 17.829904067672505 31.512520680565387 32.097412683495826 18.560162568266282 44-45 18.132345997072008 30.834163316363245 31.887559654719094 19.145931031845656 46-47 17.166012188860826 30.564498558741494 32.45008370332339 19.819405549074283 48-49 16.90368292210261 30.777543840920103 31.884055661790907 20.43471757518638 50-51 16.149281729418448 29.951374347413783 31.828779064498452 22.07056485866931 52-53 16.59062583348621 28.881453785200133 31.517456326069464 23.01046405524419 54-55 17.095398822192497 28.34874894056086 31.579602597032675 22.976249640213968 56-57 17.491832994701404 26.954145253177163 31.693239313174775 23.860782438946657 58-59 16.943439733335353 26.974876489919403 32.14479074498062 23.93689303176463 60-61 17.602240755727102 26.480930505731987 31.82627409212855 24.090554646412365 62-63 17.007707051173888 26.34057607951409 32.615705873411684 24.036010995900345 64-65 16.53304405342514 26.14606244620828 32.88803751872749 24.432855981639094 66-67 16.690122168527733 25.346288288873488 33.219884819305626 24.743704723293156 68-69 17.01796307666187 24.78235405361403 33.54390990901586 24.655772960708248 70-71 16.629742677085215 24.72654695709332 33.43269170803223 25.211018657789236 72-73 17.028289123151556 24.294166710835004 33.2581608144615 25.419383351551943 74-75 16.992250045484226 23.6428114095368 33.456803334566835 25.90813521041213 76-77 16.460127227497466 23.585389162946367 32.70349265863385 27.250990950922322 78-79 16.969170023531287 23.533242930642523 32.0494954732182 27.448091572607986 80-81 16.577916964796152 23.78024591038258 30.984053018908103 28.65778410591317 82-83 16.60378473732367 23.322725876722664 29.92347135407618 30.150018031877483 84-85 17.31943403141236 22.853165468602413 29.07481573923479 30.75258476075044 86-87 17.201025061492516 22.240318819343685 28.6956762899861 31.8629798291777 88-89 17.331240413509015 22.108108829440777 27.99877786866749 32.56187288838272 90-91 18.015556932587618 22.109392512444416 27.27089342268202 32.60415713228594 92-93 17.749687395326603 22.51701296549488 26.45116314368054 33.28213649549798 94-95 17.519578083345888 22.185185868960392 26.873126635146395 33.42210941254732 96-97 17.864506346723967 22.086305599592805 26.42286639025434 33.62632166342889 98-99 18.160928986807086 22.333655324709046 26.096467980915563 33.4089477075683 100-101 18.12513672722566 22.249167328714286 26.285355030231855 33.3403409138282 102-103 17.914313767295127 23.06401196489778 26.572209245956547 32.44946502185055 104-105 17.651559275991872 23.140539508262396 27.147208025089125 32.060693190656615 106-107 17.037472887810026 23.540606844559953 27.596030652308475 31.82588961532155 108-109 17.053751120121046 24.13460351188966 28.013904786634065 30.79774058135523 110-111 16.86510228883857 24.49312165278848 28.50655953207626 30.135216526296688 112-113 16.18339695703948 24.99095161920277 28.885434373983298 29.940217049774446 114-115 16.310776595520466 24.8355357995706 29.492905859629907 29.360781745279024 116-117 16.08259122287331 25.16480647801742 30.054335389060988 28.698266910048282 118-119 15.886176764031354 26.051413596600128 30.294002409683102 27.768407229685423 120-121 15.857984683882176 26.859146224733806 30.352714413423858 26.930154677960168 122-123 15.345005149330587 27.82900604700968 30.309510448837557 26.51647835482217 124-125 14.68451311912376 29.005918003168734 30.83337022448947 25.476198653218034 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 25.0 1 15.5 2 12.0 3 35.0 4 67.0 5 177.5 6 461.5 7 950.0 8 1729.0 9 2498.0 10 3029.5 11 3550.5 12 4195.5 13 4977.5 14 5771.5 15 6661.0 16 7781.0 17 8923.5 18 10256.0 19 11783.0 20 13434.5 21 15428.5 22 17690.0 23 20297.0 24 23496.5 25 27347.0 26 31754.0 27 36612.0 28 42113.5 29 48387.5 30 54668.5 31 60872.0 32 66711.5 33 70623.5 34 72039.5 35 71116.5 36 68746.5 37 65347.5 38 60877.5 39 55948.5 40 50868.5 41 46622.5 42 43599.0 43 43297.5 44 45501.5 45 48109.0 46 54142.5 47 59288.5 48 71631.5 49 90012.5 50 115731.5 51 130491.5 52 121330.5 53 112281.5 54 101855.0 55 87788.5 56 71987.0 57 55940.0 58 42397.0 59 32803.5 60 24726.0 61 18695.5 62 14384.5 63 10056.0 64 6602.0 65 4628.0 66 3511.5 67 2699.5 68 2102.0 69 1727.5 70 1432.0 71 1209.0 72 1054.5 73 993.0 74 755.0 75 520.0 76 444.0 77 335.0 78 232.0 79 145.0 80 116.0 81 93.5 82 62.5 83 40.0 84 25.0 85 16.0 86 10.5 87 5.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11625040050406935 2 0.025687844646903307 3 0.0 4 1.9851502818317855E-4 5 0.02064556293105057 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.9851502818317856E-5 18-19 1.1910901690990712E-4 20-21 3.3747554791140353E-4 22-23 1.9851502818317856E-5 24-25 0.0012109416719173892 26-27 0.0013499021916456141 28-29 9.131691296426213E-4 30-31 3.3747554791140353E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.962875704579464E-4 40-41 9.131691296426213E-4 42-43 0.3372373298775837 44-45 0.321892118199024 46-47 0.3195297893636442 48-49 0.32911806522489173 50-51 0.3335250988505583 52-53 0.3389247076171408 54-55 0.33860708357204766 56-57 0.34148555148070375 58-59 0.34073119437360766 60-61 0.35780348679736107 62-63 0.3516296694208642 64-65 0.35816081384809073 66-67 0.3708062211433592 68-69 0.3826377168230767 70-71 0.3679079017318848 72-73 0.3716995387701835 74-75 0.38073197255251817 76-77 0.37698003851985606 78-79 0.4526539672632837 80-81 0.6191286698976972 82-83 0.5876838894334818 84-85 0.5489734589377621 86-87 0.5044266866134567 88-89 0.33082529446726705 90-91 0.015980459768745875 92-93 0.07718264295761981 94-95 1.786635253648607E-4 96-97 0.0 98-99 0.0 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.1762416420210259 114-115 0.15960608265927553 116-117 0.15315434424332225 118-119 0.13628056684775205 120-121 0.13925829227049977 122-123 0.13203234524463206 124-125 0.0012109416719173892 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2518701.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.56272502320705 #Duplication Level Percentage of deduplicated Percentage of total 1 74.69154631399992 27.309264694369134 2 14.091525482099836 10.30449142719063 3 4.892459650143946 5.366449706260467 4 1.9825357045745524 2.8994763126019762 5 1.0083382583676939 1.843379723553875 6 0.599840436590204 1.3159080564508858 7 0.3777474832116471 0.9668034149813182 8 0.2984513597492472 0.8729755999451176 9 0.22663357933089331 0.7457707117890572 >10 1.473743056866551 10.469444888064752 >50 0.17752588857311435 4.540037322460446 >100 0.14496571033671884 10.770978883791232 >500 0.019282911345412726 4.862842510661598 >1k 0.013741844866845852 9.179606442201049 >5k 9.973919661420377E-4 2.1688764639512668 >10k+ 6.649279774280251E-4 6.38369384172717 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67260 2.6704241591201177 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25784 1.0237022973350152 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24655 0.9788776039712535 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 13390 0.5316232454745521 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 13256 0.526303042719243 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13202 0.5241590804148646 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7381 0.29304788460400816 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 6738 0.2675188519796514 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 6567 0.26072963801578675 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 6307 0.2504068565502614 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5699 0.2262674291231869 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5388 0.2139197943701932 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5228 0.2075673134683315 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5218 0.20717028341196514 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 5001 0.1985547311888152 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 4941 0.19617255085061705 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 4908 0.19486235166460808 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 4567 0.1813236267425153 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 4547 0.18052956662978256 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4158 0.16508509743713126 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3982 0.1580973684450834 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 3959 0.15718419931544078 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 3751 0.14892597414302056 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 3602 0.14301022630316185 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 3453 0.1370944784633031 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 3365 0.13360061396727915 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 3220 0.127843678149967 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3045 0.12089565216355574 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 2608 0.10354543870034594 No Hit GGTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAA 2536 0.10068682229450816 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2526 0.1002897922381418 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 7.940601127327142E-5 0.0 6 0.0 0.0 0.0 7.940601127327142E-5 0.0 7 0.0 0.0 0.0 7.940601127327142E-5 0.0 8 0.0 0.0 0.0 7.940601127327142E-5 0.0 9 0.0 0.0 0.0 1.5881202254654285E-4 0.0 10-11 1.9851502818317856E-5 0.0 0.0 2.3821803381981427E-4 0.0 12-13 3.970300563663571E-5 0.0 0.0 4.1688155918467493E-4 0.0 14-15 3.970300563663571E-5 0.0 0.0 7.146541014594427E-4 0.0 16-17 7.940601127327142E-5 0.0 0.0 0.0015881202254654284 0.0 18-19 1.1910901690990714E-4 1.9851502818317856E-5 0.0 0.002580695366381321 1.1910901690990714E-4 20-21 1.1910901690990714E-4 3.970300563663571E-5 0.0 0.005340054258127503 1.1910901690990714E-4 22-23 1.5881202254654285E-4 3.970300563663571E-5 0.0 0.020010314840864397 1.5881202254654285E-4 24-25 1.5881202254654285E-4 3.970300563663571E-5 0.0 0.05723188262521038 1.9851502818317855E-4 26-27 1.5881202254654285E-4 7.940601127327142E-5 0.0 0.0969547397646644 1.9851502818317855E-4 28-29 1.7866352536486069E-4 1.1910901690990714E-4 0.0 0.20576082671186458 1.9851502818317855E-4 30-31 1.9851502818317855E-4 1.1910901690990714E-4 0.0 0.5612019846738457 1.9851502818317855E-4 32-33 1.9851502818317855E-4 1.1910901690990714E-4 0.0 1.1647869278648002 1.9851502818317855E-4 34-35 2.3821803381981427E-4 1.1910901690990714E-4 0.0 1.5988598884901384 1.9851502818317855E-4 36-37 2.977725422747678E-4 1.1910901690990714E-4 0.0 2.0892515626110444 1.9851502818317855E-4 38-39 3.3747554791140353E-4 1.1910901690990714E-4 0.0 2.737681050668579 1.9851502818317855E-4 40-41 3.5732705072972137E-4 1.1910901690990714E-4 0.0 3.63774818845111 1.9851502818317855E-4 42-43 3.5732705072972137E-4 1.1910901690990714E-4 0.0 4.447113809856748 1.9851502818317855E-4 44-45 3.5732705072972137E-4 1.1910901690990714E-4 0.0 5.150194485173111 2.3821803381981427E-4 46-47 3.5732705072972137E-4 1.1910901690990714E-4 0.0 5.81615285021922 2.3821803381981427E-4 48-49 3.5732705072972137E-4 1.1910901690990714E-4 0.0 6.417712940122707 2.3821803381981427E-4 50-51 3.5732705072972137E-4 1.1910901690990714E-4 0.0 7.03134274373973 2.3821803381981427E-4 52-53 3.5732705072972137E-4 1.1910901690990714E-4 0.0 7.7489547191191015 2.977725422747678E-4 54-55 3.5732705072972137E-4 1.5881202254654285E-4 0.0 8.412391943307284 3.5732705072972137E-4 56-57 3.5732705072972137E-4 1.5881202254654285E-4 0.0 8.995847462640466 3.5732705072972137E-4 58-59 3.5732705072972137E-4 1.5881202254654285E-4 0.0 9.635264368418483 3.5732705072972137E-4 60-61 3.5732705072972137E-4 1.5881202254654285E-4 0.0 10.239941144264444 3.5732705072972137E-4 62-63 3.5732705072972137E-4 1.5881202254654285E-4 0.0 10.847635348538791 3.5732705072972137E-4 64-65 3.5732705072972137E-4 1.5881202254654285E-4 0.0 11.478952841166935 3.5732705072972137E-4 66-67 3.5732705072972137E-4 1.5881202254654285E-4 0.0 12.053276669203688 3.970300563663571E-4 68-69 3.5732705072972137E-4 1.5881202254654285E-4 0.0 12.640642934592076 4.367330620029928E-4 70-71 3.5732705072972137E-4 1.5881202254654285E-4 0.0 13.333142758906277 4.367330620029928E-4 72-73 3.5732705072972137E-4 1.5881202254654285E-4 0.0 13.966346144302161 4.367330620029928E-4 74-75 3.5732705072972137E-4 1.5881202254654285E-4 0.0 14.549722257624069 4.367330620029928E-4 76-77 3.5732705072972137E-4 1.5881202254654285E-4 0.0 15.127202474608936 4.367330620029928E-4 78-79 3.5732705072972137E-4 1.5881202254654285E-4 0.0 15.699005161787763 4.367330620029928E-4 80-81 3.5732705072972137E-4 1.5881202254654285E-4 0.0 16.282202611584303 4.367330620029928E-4 82-83 3.5732705072972137E-4 1.5881202254654285E-4 0.0 16.929282197450192 4.367330620029928E-4 84-85 3.5732705072972137E-4 1.5881202254654285E-4 0.0 17.517422671448497 4.367330620029928E-4 86-87 3.7717855354803926E-4 1.5881202254654285E-4 0.0 18.073979404462854 4.367330620029928E-4 88-89 3.970300563663571E-4 1.5881202254654285E-4 0.0 18.621543406700518 5.161390732762643E-4 90-91 3.970300563663571E-4 1.7866352536486069E-4 0.0 19.15554883251327 5.161390732762643E-4 92-93 3.970300563663571E-4 1.9851502818317855E-4 0.0 19.68338044094952 5.955450845495356E-4 94-95 3.970300563663571E-4 1.9851502818317855E-4 0.0 20.21160907944214 5.955450845495356E-4 96-97 3.970300563663571E-4 1.9851502818317855E-4 0.0 20.727986370752227 5.955450845495356E-4 98-99 3.970300563663571E-4 1.9851502818317855E-4 0.0 21.253138026308008 5.955450845495356E-4 100-101 3.970300563663571E-4 1.9851502818317855E-4 0.0 21.827660369372943 5.955450845495356E-4 102-103 3.970300563663571E-4 2.3821803381981427E-4 0.0 22.332444383037128 5.955450845495356E-4 104-105 3.970300563663571E-4 2.3821803381981427E-4 0.0 22.81275149372633 5.955450845495356E-4 106-107 3.970300563663571E-4 2.3821803381981427E-4 0.0 23.298081034628566 5.955450845495356E-4 108-109 3.970300563663571E-4 2.3821803381981427E-4 0.0 23.797624251548715 6.153965873678535E-4 110-111 3.970300563663571E-4 2.3821803381981427E-4 0.0 24.28045250309584 6.550995930044892E-4 112-113 3.970300563663571E-4 2.3821803381981427E-4 0.0 24.80435351397407 6.749510958228071E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 600 0.0 56.487926 1 GACCGTT 395 0.0 49.607227 7 ATAGTAC 735 0.0 49.2839 4 TAGTACT 875 0.0 44.113136 5 AGTGTAC 975 0.0 43.24305 3 ATAACGC 540 0.0 41.788082 3 GCATAGT 815 0.0 41.53996 2 TACAACG 415 0.0 40.07364 2 TCACGCA 475 0.0 40.005432 4 GTGTACT 1100 0.0 39.40876 4 TAACGCA 715 0.0 39.035023 4 AGATTAC 970 0.0 38.576042 2 CATGGGT 3315 0.0 38.334766 4 ATGGGTG 1930 0.0 38.15285 5 TTAGGAC 540 0.0 36.28965 3 GTACAAC 500 0.0 35.676586 1 GTATAAC 635 0.0 35.582947 1 TAGGACC 670 0.0 35.452576 4 ATGGGGT 4650 0.0 34.99131 5 AACGCAG 72555 0.0 34.544277 6 >>END_MODULE