FastQCFastQC Report
Fri 10 Feb 2017
SRR3557042.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557042.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3381438
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288080.851945237499549No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA145770.4310887853037672No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT128400.3797201072443144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116710.34514901648351975No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT111590.3300075293410673No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102690.30368736614422626No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG85970.25424094719465506No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT75260.22256803170722042No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC68340.2021033654912496No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT63630.18817438024887637No Hit
GTAATAAACACCCACATCCTCAGCCTCCACTCTACTGATTCTCAGTGTGA61680.18240760291923142No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC61010.18042619737519955No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG59450.17581277551148358No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT59330.17545789690658234No Hit
CAGTAATAAACACCCACATCCTCAGCCTCCACTCTACTGATTCTCAGTGT59160.17495515221630561No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG58840.17400880926990234No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA58180.17205697694294558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58000.17152465903559372No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA55650.16457495302294467No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT54720.16182464383496015No Hit
CTCCTGGAGAGTCAGTATCCATCTCCTGCAGGTCTAGTAAGAGTCTCCTG54380.16081915445440667No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA49520.14644657095590694No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG49210.14552980122657877No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT49040.14502705653630202No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT48440.14325266351179586No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT48300.14283863847274444No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG48100.14224717413124238No Hit
CTCTGTACCTGTCACTCCTGGAGAGTCAGTATCCATCTCCTGCAGGTCTA47970.14186272230926605No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA44640.13201484102325697No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC43980.13006300869630022No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT42470.12559745291795973No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA42070.12441452423495565No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC41310.12216695973724788No Hit
CTCTAGGAGAGAGAGTCACTATCACTTGCAAGGCGAGTCAGGACATTAAT41280.12207824008602258No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC40230.11897305229313683No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA39570.11702121996618006No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG39300.1162227431051523No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA36700.10853370666562569No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC36440.10776480302167302No Hit
CTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTG35640.10539894565566484No Hit
GGCCAGGCCAGTCTCCTCAGCTCCTGATATATCGGATGTCCAACCTTGCC35170.10400900445313502No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC34390.10170229352127705No Hit
TCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTCAGTCGTG34380.10167272030420195No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC34350.10158400065297664No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCAGGAACTGCTTTCACACTGAG34090.10081509700902397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4750.052.591931
TCACGCA3750.049.095824
ATAGTAC5400.048.391944
TACAACG3300.045.0051542
TAGTACT6300.042.421515
TAACGCA4800.042.0679934
CATGGGT26550.041.382944
GACCGTT3500.039.0162437
AGATTAC5600.038.1900862
AGTGTAC8500.037.031483
GTATCAC5700.036.522171
TACTGGT11600.036.361062
TATCACG5200.035.4155962
ATCACGC5250.035.0684473
ATAACGC7250.034.4053043
CATGGGG75850.033.747054
GTACTGG14200.033.5092771
GTACTAG14100.033.3250961
GTACAAC5000.033.308221
GTCTATA8500.032.8883671
Produced by FastQC (version 0.11.5)