##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557022.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2882075 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.801865322727547 33.0 32.0 34.0 27.0 34.0 2 32.035569858522074 33.0 32.0 34.0 28.0 34.0 3 32.17401594337413 33.0 32.0 34.0 28.0 34.0 4 32.014591223337355 33.0 32.0 34.0 28.0 34.0 5 32.192623023342556 33.0 33.0 34.0 30.0 34.0 6 35.57850402921506 38.0 36.0 38.0 29.0 38.0 7 35.907004848936964 38.0 37.0 38.0 31.0 38.0 8 36.09670706001752 38.0 37.0 38.0 33.0 38.0 9 36.21058508192882 38.0 37.0 38.0 33.0 38.0 10-11 36.21377601207463 38.0 37.0 38.0 33.0 38.0 12-13 36.19881300798904 38.0 37.0 38.0 33.0 38.0 14-15 36.19846985245006 38.0 37.0 38.0 33.0 38.0 16-17 36.14935437141643 38.0 37.0 38.0 33.0 38.0 18-19 36.1148212659282 38.0 37.0 38.0 33.0 38.0 20-21 36.0826425405307 38.0 37.0 38.0 32.5 38.0 22-23 36.0389915252032 38.0 37.0 38.0 32.0 38.0 24-25 35.94931967419308 38.0 37.0 38.0 31.0 38.0 26-27 35.840923640085705 38.0 37.0 38.0 31.0 38.0 28-29 35.75100543878976 38.0 37.0 38.0 30.0 38.0 30-31 35.6467767146934 38.0 37.0 38.0 29.0 38.0 32-33 35.525631012378234 38.0 37.0 38.0 29.0 38.0 34-35 35.318569780453316 38.0 37.0 38.0 27.5 38.0 36-37 35.15042061709012 38.0 36.0 38.0 27.0 38.0 38-39 34.96944042053035 38.0 36.0 38.0 25.5 38.0 40-41 34.713767511254915 38.0 36.0 38.0 24.5 38.0 42-43 34.28303756841859 38.0 35.0 38.0 16.0 38.0 44-45 33.957703564272265 38.0 35.0 38.0 15.5 38.0 46-47 33.449006358266175 38.0 34.0 38.0 15.0 38.0 48-49 33.024356409878294 38.0 33.0 38.0 15.0 38.0 50-51 32.670147029483964 38.0 31.0 38.0 15.0 38.0 52-53 32.40013150247651 38.0 29.0 38.0 15.0 38.0 54-55 32.225777087688556 38.0 28.5 38.0 15.0 38.0 56-57 32.04279954546637 38.0 27.0 38.0 15.0 38.0 58-59 31.907205398887953 38.0 26.0 38.0 15.0 38.0 60-61 31.761215270248 38.0 26.0 38.0 15.0 38.0 62-63 31.655296444402037 38.0 26.0 38.0 15.0 38.0 64-65 31.56351187946185 38.0 25.0 38.0 15.0 38.0 66-67 31.396635757223528 38.0 25.0 38.0 15.0 38.0 68-69 31.292811949723724 38.0 25.0 38.0 15.0 38.0 70-71 31.11072803448904 38.0 24.5 38.0 14.5 38.0 72-73 30.944394229851756 38.0 24.0 38.0 14.0 38.0 74-75 30.81269432613655 38.0 24.0 38.0 14.0 38.0 76-77 30.730412116270394 38.0 23.5 38.0 14.0 38.0 78-79 30.52039433394343 38.0 21.5 38.0 14.0 38.0 80-81 30.448433333622475 38.0 21.5 38.0 14.0 38.0 82-83 30.338441400726907 38.0 21.0 38.0 14.0 38.0 84-85 30.238373741141366 37.5 21.0 38.0 13.5 38.0 86-87 30.136184519833797 37.0 18.5 38.0 13.0 38.0 88-89 30.039776723367712 37.0 15.5 38.0 13.0 38.0 90-91 30.044249542430368 37.0 16.0 38.0 13.0 38.0 92-93 30.00905788364286 37.0 15.0 38.0 13.0 38.0 94-95 29.963842197028182 37.0 15.0 38.0 13.0 38.0 96-97 29.916614418431166 37.0 15.0 38.0 13.0 38.0 98-99 29.842988645333655 37.0 15.0 38.0 13.0 38.0 100-101 29.791397864385903 37.0 15.0 38.0 13.0 38.0 102-103 29.739052418830184 37.0 15.0 38.0 13.0 38.0 104-105 29.581124016550575 36.0 15.0 38.0 13.0 38.0 106-107 29.45604746580155 36.0 15.0 38.0 13.0 38.0 108-109 29.34096354189256 36.0 15.0 38.0 13.0 38.0 110-111 29.22869928783949 36.0 15.0 38.0 13.0 38.0 112-113 28.9089041749434 35.0 14.5 38.0 13.0 38.0 114-115 28.7927723948891 35.0 14.0 38.0 13.0 38.0 116-117 28.67611859510943 35.0 14.0 38.0 13.0 38.0 118-119 28.497677367868636 34.5 14.0 38.0 13.0 38.0 120-121 28.360523581100423 34.0 14.0 38.0 13.0 38.0 122-123 28.170077461551138 34.0 14.0 38.0 13.0 38.0 124-125 27.030619085207704 30.5 14.0 37.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3881854837842553 1101 2 -0.6433386073036402 1101 3 -0.3042909554983311 1101 4 -0.43042899020045766 1101 5 -0.2555729707934056 1101 6 -0.04078297141730758 1101 7 0.05786333376099151 1101 8 0.005297407991875502 1101 9 0.0217647084757715 1101 10-11 0.06652813354691034 1101 12-13 0.0840062043318639 1101 14-15 0.07676358457730004 1101 16-17 0.055917786049668905 1101 18-19 0.08839395544231365 1101 20-21 0.08640137950544613 1101 22-23 0.06664723783261906 1101 24-25 -0.027355610385065177 1101 26-27 -0.13809253593560555 1101 28-29 0.026223828300039997 1101 30-31 0.08180914480547585 1101 32-33 0.003301625804660091 1101 34-35 0.09850824299672212 1101 36-37 0.18116185439446753 1101 38-39 0.015114218908607313 1101 40-41 0.056277074304702523 1101 42-43 -0.23657026885795318 1101 44-45 -0.011054243899714322 1101 46-47 -0.15447828246254858 1101 48-49 -0.13661250040312467 1101 50-51 -0.11454765471430761 1101 52-53 -0.22781052584107186 1101 54-55 -0.26816249440176776 1101 56-57 -0.27949915750960486 1101 58-59 -0.2550130316061896 1101 60-61 -0.20089245612623685 1101 62-63 -0.19431991516554703 1101 64-65 -0.3286615360884362 1101 66-67 -0.3440306831097608 1101 68-69 -0.37842006336285294 1101 70-71 -0.4669916906982756 1101 72-73 -0.4345487207561334 1101 74-75 -0.47146182146816074 1101 76-77 -0.4869993658473284 1101 78-79 -0.3158895160470223 1101 80-81 -0.3709995702030007 1101 82-83 -0.44718700770573605 1101 84-85 -0.6028305195813317 1101 86-87 -0.41573485837793456 1101 88-89 -0.3272309697479123 1101 90-91 -0.37982617062218793 1101 92-93 -0.19456510067643862 1101 94-95 -0.22628320733124596 1101 96-97 -0.47784453942094274 1101 98-99 -0.5172019614071282 1101 100-101 -0.4605263377672344 1101 102-103 -0.46525983126616666 1101 104-105 -0.3689688775766413 1101 106-107 -0.43197449335263016 1101 108-109 -0.3903110334272739 1101 110-111 -0.5978235106479097 1101 112-113 -0.7882466314423731 1101 114-115 -0.6101175963588545 1101 116-117 -0.5172060862197903 1101 118-119 -0.48805008431040164 1101 120-121 -0.4832380519958477 1101 122-123 -0.5730280402788814 1101 124-125 -0.7166322287849631 1102 1 -0.6219623549952509 1102 2 -0.6158384595464987 1102 3 -0.40877499724238575 1102 4 -0.5178222538311772 1102 5 -0.18389416381849344 1102 6 -0.06442774774205873 1102 7 -0.181376555048125 1102 8 -0.16287008369254607 1102 9 -0.26515132380407636 1102 10-11 -0.08183499586218801 1102 12-13 -0.05039484392028726 1102 14-15 -0.1735012277129968 1102 16-17 -0.09069884181258203 1102 18-19 -0.13791348938613623 1102 20-21 -0.1443796550658476 1102 22-23 -0.10109218202922676 1102 24-25 -0.24474906052889622 1102 26-27 -0.12730220719776497 1102 28-29 -0.12225623514348882 1102 30-31 -0.12332270301144632 1102 32-33 -0.16324156675728574 1102 34-35 -0.10272614682441628 1102 36-37 -0.08459977554607434 1102 38-39 -0.10758167799663454 1102 40-41 -0.036743374799499406 1102 42-43 -0.1991966738830513 1102 44-45 -0.14209054710318014 1102 46-47 -0.19127677514590857 1102 48-49 -0.3263781503068657 1102 50-51 -0.2568565562828553 1102 52-53 -0.12992795837418925 1102 54-55 -0.14637397169222055 1102 56-57 -0.11527871935396661 1102 58-59 -0.09734259495234099 1102 60-61 -0.11838426166039895 1102 62-63 -0.060178410813737315 1102 64-65 0.057920262266179634 1102 66-67 -0.03184648372578991 1102 68-69 -0.1814785635648697 1102 70-71 -0.03921459518708659 1102 72-73 0.03266748137176023 1102 74-75 -0.16462188896168684 1102 76-77 -0.1402023894913782 1102 78-79 -0.1490734572193304 1102 80-81 -0.2955518055021322 1102 82-83 -0.2799870225298946 1102 84-85 -0.09129002055943758 1102 86-87 -0.1400725914829941 1102 88-89 0.035353191267649464 1102 90-91 -0.12490046824286338 1102 92-93 -0.03375851865281376 1102 94-95 0.02681345539199853 1102 96-97 5.911532790818796E-4 1102 98-99 0.0076341977210141465 1102 100-101 -0.14374247061883239 1102 102-103 -0.10818640613931407 1102 104-105 -0.43841758114092855 1102 106-107 -0.2816153708265894 1102 108-109 -0.13149398922899636 1102 110-111 -0.27090580459451985 1102 112-113 -0.36625235085494623 1102 114-115 -0.3316434020228485 1102 116-117 -0.11105797203694578 1102 118-119 -0.045084402239204735 1102 120-121 -0.09358714399186852 1102 122-123 -0.13696134512657565 1102 124-125 -0.41397582179299874 1103 1 -0.6063264549891585 1103 2 -0.6249869661882137 1103 3 -0.3079283222612794 1103 4 -0.11406885411776102 1103 5 -0.21426070396244512 1103 6 -0.015672398424229073 1103 7 -0.2588854250225694 1103 8 -0.12371689896777127 1103 9 -0.08264053325157761 1103 10-11 -0.06815565450919081 1103 12-13 -0.08351882860210225 1103 14-15 -0.1090802868329277 1103 16-17 -0.1364740296857505 1103 18-19 -0.220141865467248 1103 20-21 -0.1915200498051064 1103 22-23 -0.13939688971918685 1103 24-25 -0.3078767557481612 1103 26-27 -0.24298431960141187 1103 28-29 -0.24589538223267482 1103 30-31 -0.20526232719737436 1103 32-33 -0.26235179971325095 1103 34-35 -0.30303173401333794 1103 36-37 -0.21206659356912638 1103 38-39 -0.38006907087723363 1103 40-41 -0.3553730181718606 1103 42-43 -0.3592202529697488 1103 44-45 -0.39728681131280297 1103 46-47 -0.46855799373091145 1103 48-49 -0.5000746607568445 1103 50-51 -0.32753201439152235 1103 52-53 -0.5502810544717889 1103 54-55 -0.5608275188595897 1103 56-57 -0.558275043648166 1103 58-59 -0.592145423668704 1103 60-61 -0.5249601128828445 1103 62-63 -0.45958178590311505 1103 64-65 -0.4172195601698476 1103 66-67 -0.45187690335999875 1103 68-69 -0.31386795133103007 1103 70-71 -0.37622385604996467 1103 72-73 -0.5154407008712596 1103 74-75 -0.6808320877632852 1103 76-77 -0.6118780357876616 1103 78-79 -0.44894190729833383 1103 80-81 -0.47957783296301315 1103 82-83 -0.6779493593654209 1103 84-85 -0.47451905650665793 1103 86-87 -0.37310474399140503 1103 88-89 -0.305381785409665 1103 90-91 -0.5023296729300419 1103 92-93 -0.4122817755468198 1103 94-95 -0.38574612122938845 1103 96-97 -0.37247653717985685 1103 98-99 -0.4584691719501066 1103 100-101 -0.4767320632143992 1103 102-103 -0.5260844761760701 1103 104-105 -0.6385832370449407 1103 106-107 -0.5004949917035155 1103 108-109 -0.5331217516926756 1103 110-111 -0.6408209290740956 1103 112-113 -0.7804193231047698 1103 114-115 -0.6156346704074203 1103 116-117 -0.389063156630133 1103 118-119 -0.6276234814066903 1103 120-121 -0.5059565978994485 1103 122-123 -0.8002138658247944 1103 124-125 -0.906573009331396 1104 1 -0.11617178237908732 1104 2 -0.04834721469380909 1104 3 -0.23568099063330195 1104 4 -0.3507821762185017 1104 5 -0.07446898268836577 1104 6 0.10914564977793617 1104 7 0.05928736445002869 1104 8 -0.0017574610793360534 1104 9 0.0153137809914341 1104 10-11 -0.01859938920817683 1104 12-13 0.022817080023941116 1104 14-15 -0.00608699281652747 1104 16-17 -0.021645612840664796 1104 18-19 0.06413005204068867 1104 20-21 0.04224041269032597 1104 22-23 0.016768814697208256 1104 24-25 -0.07430283848267294 1104 26-27 -0.12477641860515831 1104 28-29 -0.03076276725311544 1104 30-31 -0.03403944386005264 1104 32-33 -0.023099240545874977 1104 34-35 -0.06238112334018808 1104 36-37 -0.08033990137620606 1104 38-39 -0.009162215669668683 1104 40-41 -0.17022663327313836 1104 42-43 -0.2663058341344424 1104 44-45 -0.13964282140821638 1104 46-47 -0.22809069304648943 1104 48-49 -0.18128082705337079 1104 50-51 -0.07422507899879349 1104 52-53 -0.20853983787702646 1104 54-55 -0.30082589269305515 1104 56-57 -0.30696077268169475 1104 58-59 -0.3145569041114271 1104 60-61 -0.07004759441602815 1104 62-63 -0.0709268246240704 1104 64-65 -0.1956733541494451 1104 66-67 -0.17525468321050042 1104 68-69 -0.2495642967170788 1104 70-71 -0.2337920558506461 1104 72-73 -0.2262463333163609 1104 74-75 -0.3439586883683603 1104 76-77 -0.27868542527395945 1104 78-79 -0.24160237801005735 1104 80-81 -0.32999285337006157 1104 82-83 -0.38491378379830365 1104 84-85 -0.4114016977038766 1104 86-87 -0.350713883633329 1104 88-89 -0.3326391810795464 1104 90-91 -0.30899910440537326 1104 92-93 -0.2058064219196929 1104 94-95 -0.22541678343931792 1104 96-97 -0.14960815521677873 1104 98-99 -0.08611982645161476 1104 100-101 -0.10530036642072105 1104 102-103 -0.28283077820378466 1104 104-105 -0.21516005394115112 1104 106-107 0.011895799066351742 1104 108-109 -0.1403751869389538 1104 110-111 -0.23284651116419397 1104 112-113 -0.3287203148099813 1104 114-115 -0.2567088901374355 1104 116-117 -0.19033205486446292 1104 118-119 -0.20075519369290618 1104 120-121 0.05389176329216383 1104 122-123 -0.19631238465811052 1104 124-125 -0.36872947707305315 1105 1 0.06294291396288543 1105 2 0.042507082359776405 1105 3 -0.12193749942917265 1105 4 -0.04800876901798645 1105 5 -0.12383909426704776 1105 6 -0.06772148974918935 1105 7 -0.17659065412560437 1105 8 -0.23191400742498303 1105 9 -0.06478080998687119 1105 10-11 -0.11028442056242227 1105 12-13 -0.16275715834763815 1105 14-15 -0.19419575284628365 1105 16-17 -0.19580565045419718 1105 18-19 -0.11368993825373508 1105 20-21 -0.2396948139971471 1105 22-23 -0.2320054464332415 1105 24-25 -0.32273453606918423 1105 26-27 -0.12087943367193787 1105 28-29 -0.1574881418980567 1105 30-31 -0.19768542485898877 1105 32-33 -0.1007769996845056 1105 34-35 -0.12355468560323146 1105 36-37 -0.23929835710635672 1105 38-39 -0.182352463209007 1105 40-41 -0.08130566003853801 1105 42-43 -0.18379960525029304 1105 44-45 -0.23107718643387187 1105 46-47 -0.2391998092148384 1105 48-49 -0.3394494507116619 1105 50-51 -0.28019866949796324 1105 52-53 -0.39640041050115116 1105 54-55 -0.2764195386705097 1105 56-57 -0.33537613534635113 1105 58-59 -0.4482268043560289 1105 60-61 -0.37610197419481395 1105 62-63 -0.2661168641699696 1105 64-65 -0.34675048748988146 1105 66-67 -0.36504556953287803 1105 68-69 -0.42571024672948354 1105 70-71 -0.4114726362396972 1105 72-73 -0.45274877015664927 1105 74-75 -0.38057710220943974 1105 76-77 -0.5216851534187406 1105 78-79 -0.35985756801891355 1105 80-81 -0.7059097154880014 1105 82-83 -0.44605618245845946 1105 84-85 -0.5768018708271363 1105 86-87 -0.6052737148588072 1105 88-89 -0.4358488433074079 1105 90-91 -0.44215374280665287 1105 92-93 -0.40673410571677593 1105 94-95 -0.33455042229400433 1105 96-97 -0.548834388926565 1105 98-99 -0.3467779852186048 1105 100-101 -0.42004112833067353 1105 102-103 -0.44114447787707434 1105 104-105 -0.40260641034345923 1105 106-107 -0.30159346088188954 1105 108-109 -0.17673815367145806 1105 110-111 -0.3252267623640819 1105 112-113 -0.39701245847749433 1105 114-115 -0.054150783297981064 1105 116-117 -0.19231017636261427 1105 118-119 -0.11487048792748311 1105 120-121 -0.18953711363415238 1105 122-123 -0.3940486322132699 1105 124-125 -0.37474151448684623 1106 1 0.383108912185552 1106 2 0.24629223403197642 1106 3 0.07267853046482742 1106 4 0.14917053738771457 1106 5 0.05989662834503662 1106 6 0.2408654087484834 1106 7 0.2236423285664415 1106 8 0.18486743862403898 1106 9 0.23775507510612925 1106 10-11 0.08275232284690048 1106 12-13 0.24091648223647866 1106 14-15 0.20005936397966906 1106 16-17 0.07530714149397255 1106 18-19 0.03391634745432981 1106 20-21 0.09517850847299769 1106 22-23 0.12849908482332495 1106 24-25 0.17720865786361628 1106 26-27 0.16456327235121648 1106 28-29 0.02171126607377971 1106 30-31 0.008691406358508402 1106 32-33 0.12478199536168688 1106 34-35 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8 -0.1735181419877989 2315 9 -0.26763469448354016 2315 10-11 -0.2967270596623166 2315 12-13 -0.18714674504419548 2315 14-15 -0.2223316615750548 2315 16-17 -0.20936911370417732 2315 18-19 -0.2726451706677864 2315 20-21 -0.18509061365575263 2315 22-23 -0.18462143551830223 2315 24-25 -0.23942172316100852 2315 26-27 -0.21836758645213905 2315 28-29 -0.25768374542101924 2315 30-31 -0.09568141995129764 2315 32-33 -0.1272845206892228 2315 34-35 -0.15052882692392444 2315 36-37 -0.25018391902579395 2315 38-39 -0.2031903464159157 2315 40-41 -0.13855385347692106 2315 42-43 -0.08422499224852231 2315 44-45 -0.02506666616382347 2315 46-47 -0.11492635963343645 2315 48-49 0.0057418013490107 2315 50-51 -0.05990497098370895 2315 52-53 -0.2672764418860183 2315 54-55 -0.2790236796866452 2315 56-57 -0.24017506178563863 2315 58-59 -0.10449717264699387 2315 60-61 0.07685587102129077 2315 62-63 -0.07524073768354356 2315 64-65 -0.12678098338902188 2315 66-67 0.06606801421870401 2315 68-69 -0.02703099223964145 2315 70-71 -0.05434749742256173 2315 72-73 0.01201315951280435 2315 74-75 -0.23369421988957484 2315 76-77 -0.12456443229533676 2315 78-79 0.05729222021595959 2315 80-81 -0.016372200018427208 2315 82-83 -0.0936338722220107 2315 84-85 -0.2416078302529634 2315 86-87 -0.044279765468917986 2315 88-89 -0.15821298195958633 2315 90-91 -0.1044501533002915 2315 92-93 0.04679995303764173 2315 94-95 -0.24566369583336112 2315 96-97 -0.0754890909116881 2315 98-99 -0.0240034154201183 2315 100-101 -0.07645216767407703 2315 102-103 -0.07047931155068099 2315 104-105 -0.23792068645701647 2315 106-107 -0.11665427549691643 2315 108-109 -0.1671731447947451 2315 110-111 -0.02848052626291775 2315 112-113 -0.09357780915372871 2315 114-115 -0.07362978979919177 2315 116-117 0.02120136596612454 2315 118-119 -0.05980929064190832 2315 120-121 0.028716656491575066 2315 122-123 0.037121616215770814 2315 124-125 0.10860608974077834 2316 1 -0.17618274599547235 2316 2 -0.16498601131118207 2316 3 0.03111270478435557 2316 4 0.22843913041323205 2316 5 0.19137985519344625 2316 6 -0.09738675449787593 2316 7 -0.20326220442336052 2316 8 -0.3633115034099603 2316 9 -0.21168274132933362 2316 10-11 -0.3731413690461878 2316 12-13 -0.2536907944175084 2316 14-15 -0.17569960751330882 2316 16-17 -0.155538715066335 2316 18-19 -0.05097597568518353 2316 20-21 -0.24089762074870436 2316 22-23 -0.23911331636829658 2316 24-25 -0.16468195078179093 2316 26-27 -0.0823723062431796 2316 28-29 -0.23159031912284433 2316 30-31 0.009323922070763047 2316 32-33 -0.10450459511576327 2316 34-35 -0.10265116525841478 2316 36-37 -0.3794738762655001 2316 38-39 -0.3045916323600082 2316 40-41 -0.20457877407011438 2316 42-43 -0.1511740947164668 2316 44-45 -0.16672660003369089 2316 46-47 -0.17052083142442598 2316 48-49 -0.16343075199282708 2316 50-51 -0.1316642447581131 2316 52-53 -0.07512017025565143 2316 54-55 -0.07013216735176542 2316 56-57 0.019034439537200853 2316 58-59 0.021434184178371396 2316 60-61 0.036531141197368555 2316 62-63 -0.01881324809626861 2316 64-65 0.01488956217251669 2316 66-67 -0.05474699987796683 2316 68-69 -0.038914941332460984 2316 70-71 -0.0047701562783792895 2316 72-73 0.20370715932468997 2316 74-75 0.05522331520738888 2316 76-77 0.16634442292019003 2316 78-79 0.06013482712272378 2316 80-81 0.16878511831061616 2316 82-83 -0.058145963521145205 2316 84-85 -0.03332528431518966 2316 86-87 0.10396375838447725 2316 88-89 0.04243782826718956 2316 90-91 -0.06224751581256527 2316 92-93 -0.08642621828812125 2316 94-95 -0.17679188748051544 2316 96-97 -0.07688398572329191 2316 98-99 -0.32830862696660645 2316 100-101 -0.37901893089106053 2316 102-103 -0.11021165574572223 2316 104-105 -0.3328567389559538 2316 106-107 -0.2584348570658541 2316 108-109 -0.2926941426796432 2316 110-111 -0.22448474199860868 2316 112-113 -0.24034709168278212 2316 114-115 -0.26453888618952703 2316 116-117 -0.26041502379166204 2316 118-119 -0.5904068977054351 2316 120-121 -0.5098646206255211 2316 122-123 -0.3776855524643068 2316 124-125 -0.2795156890681554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 9.0 10 14.0 11 25.0 12 62.0 13 205.0 14 566.0 15 3652.0 16 7433.0 17 9236.0 18 14288.0 19 23925.0 20 38993.0 21 65127.0 22 103618.0 23 152603.0 24 163622.0 25 118773.0 26 74969.0 27 62521.0 28 53694.0 29 54381.0 30 60187.0 31 71353.0 32 89340.0 33 117424.0 34 159527.0 35 222729.0 36 363860.0 37 849938.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.87824186268842 15.57521871803993 10.597437914091334 22.949101505180312 2 14.43643266654106 23.38946435591586 37.38779954385954 24.78630343368354 3 20.639513399386196 32.66226815427294 25.852288400383756 20.845930045957108 4 12.903475407196751 17.822383035244005 34.75434955041981 34.51979200713943 5 13.249495096712401 39.27828325941965 30.682990137902795 16.789231505965148 6 28.41466651631203 34.12350476653106 20.69231369759635 16.76951501956056 7 26.273813138103623 33.96490375857672 21.958311286139327 17.802971817180328 8 29.039771692270328 28.81535005161212 22.185855676899457 19.959022579218097 9 31.483393041471857 14.601736596028903 20.69779585888639 33.21707450361285 10-11 24.140992167101828 26.811186384809556 26.50241579417607 22.545405653912546 12-13 26.89052158601008 24.864411925435668 28.958181171551743 19.286885317002504 14-15 25.372674201747003 23.118274160110335 29.811941049417523 21.69711058872514 16-17 20.487322502016774 27.005456138372523 30.017522097794124 22.489699261816572 18-19 20.9298161883532 25.274548165049676 34.622952002799394 19.17268364379773 20-21 22.459745352150208 24.83488798024051 33.730873262981994 18.97449340462728 22-23 20.97276940310794 26.865442743333308 31.553461304988893 20.608326548569856 24-25 20.167747807170866 27.93912141799741 32.076207871128496 19.81692290370323 26-27 21.01968046375798 27.15017120801188 32.20426433469158 19.62588399353856 28-29 20.554704509771607 27.810986875775267 32.53660990779213 19.097698706661 30-31 21.577078716012544 27.91620511530471 31.774222229932818 18.73249393874993 32-33 19.062272841615847 28.627134963524544 32.22605241015587 20.08453978470373 34-35 18.5085225054865 29.76112696581456 31.770911582800586 19.959438945898356 36-37 19.966829454472908 29.868150551252135 30.605744125326375 19.559275868948585 38-39 19.851912250722137 29.261278766166736 30.48666325477304 20.40014572833809 40-41 19.03250123396603 29.934324380826638 30.382750672934378 20.650423712272953 42-43 19.87297726604486 29.51539635233716 30.135721729569102 20.475904652048875 44-45 20.01378118052981 28.926214320841957 30.26392126252453 20.7960832361037 46-47 19.081231816557445 28.592801192273605 31.1413451771969 21.18462181397205 48-49 19.03728608537254 28.89558083042366 30.655721531713382 21.411411552490417 50-51 17.99276666401008 28.576079666926873 30.530514244110474 22.900639424952573 52-53 18.245999402479068 27.605815058397493 30.13238551271002 24.015800026413416 54-55 18.740809600858682 26.995332451867103 30.288092583885177 23.975765363389034 56-57 19.324326887586952 25.73662088963377 30.57200089724539 24.367051325533886 58-59 18.75473329286832 25.761990544551423 31.302466463227884 24.180809699352373 60-61 19.286164614812503 25.520678259510728 30.89598685551138 24.29717027016539 62-63 18.451461880027644 25.69337001816898 31.572926424236076 24.282241677567303 64-65 18.192105281935444 25.780676905427015 31.444464381216452 24.582753431421093 66-67 18.85102864900616 25.377867511400424 31.185470115222614 24.5856337243708 68-69 19.135562342407162 25.17081858028556 31.1781569433617 24.515462133945576 70-71 18.626436506520573 25.33394833145498 31.006540156911548 25.0330750051129 72-73 19.225644497026096 25.216187110529443 30.61368924233165 24.944479150112816 74-75 19.54263383726294 24.766860753544478 30.41964542155148 25.270859987641103 76-77 19.03593738849523 25.044480067951554 29.789809704330693 26.129772839222525 78-79 19.239921111869403 25.1627098152924 29.24047634339486 26.356892729443338 80-81 18.78700542126914 25.779061041348385 28.1787201143773 27.255213423005177 82-83 18.941269813590946 25.638237396899576 27.119884213646746 28.30060857586273 84-85 19.540500001046 25.415086918956764 26.607001771920807 28.43741130807643 86-87 19.748055790783344 25.013502239567988 26.320295953057695 28.918146016590978 88-89 19.96471325649067 25.23297986264655 25.89103464741273 28.911272233450052 90-91 20.463179380335003 25.714912321034433 25.338160056035697 28.48374824259487 92-93 20.080878119185225 26.603723262018157 24.64250961445631 28.672889004340306 94-95 19.84319278408535 26.86128114684078 25.126749000893106 28.168777068180763 96-97 20.270326067156475 27.10326761100943 24.724877041714738 27.90152928011936 98-99 20.536995140132568 27.6237654508931 24.465172569272582 27.37406683970175 100-101 20.46587961798357 27.71928211444879 24.584370635739873 27.230467631827764 102-103 20.195761734167224 28.618269822957416 24.91343910203586 26.2725293408395 104-105 20.13410476826592 28.68631107795599 25.29335634915816 25.88622780461994 106-107 19.338618877024366 29.239523607123342 25.80638949368077 25.615468022171527 108-109 19.147714754126802 29.74821959872661 25.86799441374704 25.23607123339955 110-111 18.811533357043103 29.94415481901061 26.38446258338176 24.859849240564525 112-113 18.370616445538104 30.02375177955761 26.430067015874688 25.175564759029594 114-115 18.408183516490777 29.735880558654827 27.000340388780312 24.855595536074084 116-117 18.366657650698205 29.933026883114806 27.290171875880016 24.410143590306976 118-119 18.032958686935924 30.430597145920917 27.454652260131006 24.08179190701215 120-121 17.90519860135922 31.078947981311135 27.52545977413043 23.490393643199216 122-123 17.479455663716568 31.84132306776885 27.30655444614054 23.372666822374036 124-125 16.945390815808548 32.83601561521425 27.645910339857483 22.57268322911972 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 61.0 1 44.5 2 42.5 3 99.0 4 163.5 5 328.0 6 684.0 7 1215.5 8 2163.0 9 2905.0 10 3118.5 11 3456.0 12 3974.0 13 4593.0 14 5286.5 15 6091.0 16 7140.5 17 8416.0 18 9835.5 19 11354.0 20 13200.5 21 15414.5 22 17856.0 23 20843.0 24 24364.5 25 28408.0 26 33468.0 27 39303.5 28 45507.0 29 51893.5 30 57924.0 31 63140.0 32 67080.5 33 68015.5 34 66095.0 35 62669.0 36 59047.5 37 55972.5 38 52669.5 39 49599.5 40 47261.0 41 46144.5 42 46066.5 43 48979.0 44 54352.0 45 59541.0 46 68241.0 47 79023.0 48 104595.5 49 131374.0 50 159944.5 51 167797.5 52 136092.0 53 117313.0 54 112538.0 55 102736.5 56 87778.5 57 71617.5 58 58847.0 59 48662.5 60 38646.5 61 30914.5 62 24554.5 63 17821.5 64 12345.0 65 9297.0 66 7212.5 67 5519.0 68 4321.0 69 3457.0 70 2905.0 71 2501.5 72 2160.5 73 2018.0 74 1587.5 75 1206.5 76 1031.0 77 755.5 78 513.5 79 338.5 80 213.5 81 144.0 82 108.0 83 76.0 84 53.5 85 29.5 86 15.0 87 8.5 88 3.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12085042894442373 2 0.002359411188119671 3 0.0013878889341880415 4 0.001838952837799155 5 0.011623569823824849 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.459903021260724E-4 20-21 5.725041853525671E-4 22-23 1.561375050961547E-4 24-25 3.643208452243609E-4 26-27 0.0018909986728312066 28-29 0.0 30-31 0.001301145875801289 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.003729951510630362 42-43 0.2838232870414545 44-45 0.272911010296401 46-47 0.2858357259960272 48-49 0.29386813320264044 50-51 0.30228220986615545 52-53 0.2961581499440507 54-55 0.3064458766687196 56-57 0.3066020141738157 58-59 0.30571723497827086 60-61 0.30462427244259777 62-63 0.3043466946557602 64-65 0.3158661728095209 66-67 0.32528646895032226 68-69 0.3248874508817432 70-71 0.32745504540999104 72-73 0.3298144565981107 74-75 0.334828205372865 76-77 0.32754178846837784 78-79 0.3261365509225124 80-81 0.5472446067503448 82-83 0.5109166138979728 84-85 0.4857958241891692 86-87 0.4547591578983892 88-89 0.3038782821404717 90-91 0.011814404552275705 92-93 0.08872080011797057 94-95 3.4697223354701044E-5 96-97 0.0 98-99 1.7348611677350522E-5 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.23287041454507604 114-115 0.20651787340718059 116-117 0.19767008145173182 118-119 0.17834372804316337 120-121 0.1832707337595309 122-123 0.16942654164100518 124-125 4.857611269658145E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2882075.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.366305579581315 #Duplication Level Percentage of deduplicated Percentage of total 1 69.1887495442711 25.853279581403516 2 15.978522117907861 11.94116680335688 3 6.762533963208169 7.580727316846008 4 3.015293943610086 4.506815796367813 5 1.4527666325176496 2.7142260963236904 6 0.8037474689637583 1.8019844120468902 7 0.5103422541181162 1.334872323228491 8 0.3233953600646596 0.9667271877755839 9 0.25178985303095003 0.8467610931173111 >10 1.4012289243016565 9.996679275704695 >50 0.15852344577867242 4.133038347773728 >100 0.12522215157897912 9.416473315371041 >500 0.013988799656341363 3.630951095274663 >1k 0.012496661026331205 8.48936410252227 >5k 8.393279793804542E-4 2.311631654345024 >10k+ 5.595519862536361E-4 4.475301598542481 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45045 1.5629364260125085 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 19495 0.6764223692998967 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19105 0.6628904521915634 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17858 0.6196230146682512 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 16576 0.5751411743275244 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 10347 0.35901217005109165 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9875 0.3426350806276728 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 8933 0.3099502962275444 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 8553 0.296765351352758 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 8216 0.28507238708222377 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 8101 0.2810822063964331 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6483 0.22494209900852682 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 5651 0.19607400917741558 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 5367 0.18621999774468048 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 5157 0.17893358084019326 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4839 0.16789986381339833 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 4567 0.15846221906091965 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 4548 0.15780297181718034 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 4328 0.1501695826791461 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4121 0.142987257444723 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 4116 0.14281377132794948 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 3984 0.13823373784512893 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3908 0.13559674887017167 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 3758 0.13039216536696652 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 3739 0.12973291812322718 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 3659 0.1269571402548511 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3526 0.12234240954867588 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3222 0.11179445364884674 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 3179 0.11030247304459462 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 2927 0.10155877275920995 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 2919 0.10128119497237234 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.469722335470104E-5 0.0 5 0.0 0.0 0.0 3.469722335470104E-5 0.0 6 0.0 0.0 0.0 3.469722335470104E-5 0.0 7 0.0 0.0 0.0 3.469722335470104E-5 0.0 8 0.0 0.0 0.0 6.939444670940207E-5 0.0 9 0.0 0.0 0.0 6.939444670940207E-5 0.0 10-11 0.0 0.0 0.0 4.8576112696581457E-4 0.0 12-13 0.0 0.0 0.0 9.71522253931629E-4 0.0 14-15 0.0 0.0 0.0 0.001266448652446588 0.0 16-17 0.0 0.0 0.0 0.002723732033344032 0.0 18-19 0.0 0.0 0.0 0.005031097386431651 0.0 20-21 0.0 0.0 0.0 0.01035712117137826 0.0 22-23 0.0 0.0 0.0 0.027497549508600574 0.0 24-25 0.0 0.0 0.0 0.06986285922469054 0.0 26-27 0.0 0.0 0.0 0.1281541944605883 0.0 28-29 0.0 0.0 0.0 0.2628835127468924 0.0 30-31 0.0 0.0 0.0 0.5564046737159859 0.0 32-33 3.469722335470104E-5 0.0 0.0 1.0185022943538944 0.0 34-35 3.469722335470104E-5 0.0 0.0 1.4030863180174007 0.0 36-37 3.469722335470104E-5 0.0 0.0 1.8858114379396786 0.0 38-39 3.469722335470104E-5 0.0 0.0 2.4891614548545755 0.0 40-41 3.469722335470104E-5 0.0 0.0 3.385772403563405 0.0 42-43 3.469722335470104E-5 0.0 0.0 4.139222608710738 0.0 44-45 3.469722335470104E-5 0.0 0.0 4.781745790793091 0.0 46-47 3.469722335470104E-5 0.0 0.0 5.420764553316621 0.0 48-49 3.469722335470104E-5 0.0 0.0 6.001162356982382 0.0 50-51 3.469722335470104E-5 0.0 0.0 6.571081599195025 0.0 52-53 3.469722335470104E-5 0.0 0.0 7.24044308354224 0.0 54-55 3.469722335470104E-5 0.0 0.0 7.837322068301484 0.0 56-57 3.469722335470104E-5 0.0 0.0 8.402158167292662 0.0 58-59 3.469722335470104E-5 0.0 0.0 9.0074685773271 0.0 60-61 3.469722335470104E-5 0.0 0.0 9.573467033300659 0.0 62-63 3.469722335470104E-5 0.0 0.0 10.122949610957384 0.0 64-65 3.469722335470104E-5 0.0 0.0 10.695089475464727 0.0 66-67 3.469722335470104E-5 0.0 0.0 11.23801427790741 0.0 68-69 3.469722335470104E-5 0.0 0.0 11.850940728468204 0.0 70-71 3.469722335470104E-5 0.0 0.0 12.547782413712342 0.0 72-73 3.469722335470104E-5 0.0 0.0 13.149865981974791 0.0 74-75 3.469722335470104E-5 0.0 0.0 13.71090273500863 0.0 76-77 3.469722335470104E-5 0.0 0.0 14.283320177302812 0.0 78-79 3.469722335470104E-5 0.0 0.0 14.826401117250592 0.0 80-81 3.469722335470104E-5 0.0 0.0 15.401177970732892 0.0 82-83 3.469722335470104E-5 0.0 0.0 16.05395418231656 0.0 84-85 3.469722335470104E-5 0.0 0.0 16.623370314790556 0.0 86-87 3.469722335470104E-5 0.0 0.0 17.17698186202649 0.0 88-89 3.469722335470104E-5 0.0 0.0 17.727349218878757 0.0 90-91 3.469722335470104E-5 0.0 0.0 18.265936868402108 0.0 92-93 3.469722335470104E-5 0.0 0.0 18.78320307417399 0.0 94-95 3.469722335470104E-5 0.0 0.0 19.317731148564835 0.0 96-97 3.469722335470104E-5 0.0 0.0 19.847661840861186 0.0 98-99 3.469722335470104E-5 0.0 0.0 20.39091626692574 0.0 100-101 3.469722335470104E-5 0.0 0.0 20.998273813138105 0.0 102-103 3.469722335470104E-5 0.0 0.0 21.53306211670411 0.0 104-105 3.469722335470104E-5 0.0 0.0 22.04734436126749 0.0 106-107 3.469722335470104E-5 0.0 0.0 22.552848208322132 0.0 108-109 3.469722335470104E-5 0.0 0.0 23.05290459131008 0.0 110-111 3.469722335470104E-5 0.0 0.0 23.551382250635392 0.0 112-113 3.469722335470104E-5 0.0 0.0 24.081417034601806 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 915 0.0 68.883415 1 TACAACG 600 0.0 56.419044 2 ATAACGC 905 0.0 53.808678 3 TAACGCA 945 0.0 52.158585 4 TAGTACT 1290 0.0 50.637814 5 ATAGTAC 1275 0.0 50.30291 4 AGATTAC 1565 0.0 49.711754 2 GCATAGT 1360 0.0 47.59811 2 GTATAAC 1070 0.0 47.235123 1 GATTACT 1850 0.0 42.373005 3 AGTGTAC 1205 0.0 41.397995 3 TCACGCA 650 0.0 41.112957 4 TATCACG 665 0.0 40.187687 2 ACGGTAC 770 0.0 40.105106 3 GTACAAC 920 0.0 40.07133 1 ACAACGC 870 0.0 39.59094 3 GACCGTT 495 0.0 38.387882 7 CATAGTA 1740 0.0 36.51923 3 CATGGGG 9385 0.0 35.751453 4 ATCACGC 790 0.0 35.331154 3 >>END_MODULE