##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3557011.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2293384 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.543100501267997 33.0 32.0 34.0 27.0 34.0 2 31.906811070453095 33.0 32.0 34.0 27.0 34.0 3 32.06850139357386 33.0 32.0 34.0 28.0 34.0 4 31.896877714329566 33.0 32.0 34.0 28.0 34.0 5 32.07414676303663 33.0 32.0 34.0 30.0 34.0 6 35.403706487879916 38.0 36.0 38.0 29.0 38.0 7 35.7471465746687 38.0 36.0 38.0 31.0 38.0 8 35.94602081465642 38.0 37.0 38.0 31.0 38.0 9 36.05384619409571 38.0 37.0 38.0 31.0 38.0 10-11 36.039550070986806 38.0 37.0 38.0 31.5 38.0 12-13 36.02767242642314 38.0 37.0 38.0 31.0 38.0 14-15 36.038340068649646 38.0 37.0 38.0 31.0 38.0 16-17 35.9786854272987 38.0 37.0 38.0 31.0 38.0 18-19 35.940555964461254 38.0 37.0 38.0 31.0 38.0 20-21 35.91684231685579 38.0 37.0 38.0 31.0 38.0 22-23 35.85453940552476 38.0 37.0 38.0 31.0 38.0 24-25 35.749016518821094 38.0 37.0 38.0 29.5 38.0 26-27 35.629521920445946 38.0 37.0 38.0 29.0 38.0 28-29 35.51682426492903 38.0 37.0 38.0 29.0 38.0 30-31 35.39437028426116 38.0 36.0 38.0 28.5 38.0 32-33 35.249495941368735 38.0 36.0 38.0 27.5 38.0 34-35 35.01547843710429 38.0 36.0 38.0 26.5 38.0 36-37 34.81240756018181 38.0 36.0 38.0 25.0 38.0 38-39 34.604023137860906 38.0 35.5 38.0 24.5 38.0 40-41 34.294770086474834 38.0 35.0 38.0 16.0 38.0 42-43 33.808892666906196 38.0 34.0 38.0 16.0 38.0 44-45 33.40125966693759 38.0 33.5 38.0 15.0 38.0 46-47 32.79349882095628 38.0 31.5 38.0 15.0 38.0 48-49 32.26363618129367 38.0 28.5 38.0 15.0 38.0 50-51 31.845772884087445 38.0 26.5 38.0 15.0 38.0 52-53 31.505421900562663 38.0 25.5 38.0 15.0 38.0 54-55 31.29900945502367 38.0 25.0 38.0 15.0 38.0 56-57 31.08231525989542 38.0 24.5 38.0 15.0 38.0 58-59 30.91588783212929 38.0 24.0 38.0 15.0 38.0 60-61 30.742276478775466 37.5 24.0 38.0 15.0 38.0 62-63 30.612687408650274 37.0 24.0 38.0 15.0 38.0 64-65 30.501370899945236 37.0 20.0 38.0 15.0 38.0 66-67 30.311943180906468 37.0 16.0 38.0 15.0 38.0 68-69 30.18349129495976 37.0 16.0 38.0 14.5 38.0 70-71 29.983514099688495 37.0 15.5 38.0 14.0 38.0 72-73 29.79312426527786 37.0 15.0 38.0 14.0 38.0 74-75 29.644114766650503 37.0 15.0 38.0 14.0 38.0 76-77 29.531705549528557 37.0 15.0 38.0 14.0 38.0 78-79 29.320274101502406 36.0 15.0 38.0 14.0 38.0 80-81 29.25157125889079 36.0 15.0 38.0 14.0 38.0 82-83 29.130007665528318 36.0 14.0 38.0 14.0 38.0 84-85 29.023281753077548 36.0 14.0 38.0 13.0 38.0 86-87 28.924155963414762 36.0 14.0 38.0 13.0 38.0 88-89 28.822031330121774 36.0 14.0 38.0 13.0 38.0 90-91 28.798405761965725 36.0 14.0 38.0 13.0 38.0 92-93 28.744304704314672 35.5 14.0 38.0 13.0 38.0 94-95 28.687094921740098 35.0 14.0 38.0 13.0 38.0 96-97 28.624451901644033 35.0 14.0 38.0 13.0 38.0 98-99 28.52676437962417 35.0 14.0 38.0 13.0 38.0 100-101 28.448311098359454 35.0 14.0 38.0 13.0 38.0 102-103 28.360865210536048 35.0 14.0 38.0 13.0 38.0 104-105 28.18061846598738 34.0 14.0 38.0 13.0 38.0 106-107 28.03544064142769 34.0 14.0 38.0 13.0 38.0 108-109 27.894722166021914 34.0 14.0 38.0 13.0 38.0 110-111 27.779002338901815 34.0 14.0 38.0 13.0 38.0 112-113 27.455861730961757 33.5 14.0 38.0 13.0 38.0 114-115 27.33690127776247 33.0 14.0 38.0 13.0 38.0 116-117 27.224830861294926 33.0 14.0 38.0 13.0 38.0 118-119 27.04119283992563 31.5 14.0 38.0 13.0 38.0 120-121 26.906840938979254 31.0 14.0 38.0 13.0 38.0 122-123 26.734980273691626 31.0 14.0 38.0 13.0 38.0 124-125 25.690991129265747 27.0 14.0 36.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.219077481671345 1101 2 -0.4180459405050776 1101 3 8.218258339596218E-4 1101 4 -0.14528676899137594 1101 5 -0.007479983421973202 1101 6 0.33033635794388516 1101 7 0.2189521254771023 1101 8 0.24629013574470804 1101 9 0.15735719754870559 1101 10-11 0.1369877128179553 1101 12-13 0.20078292984577928 1101 14-15 0.2661213446350601 1101 16-17 0.1893778308197298 1101 18-19 0.24138111569977383 1101 20-21 0.11077970121142044 1101 22-23 0.1726013431262743 1101 24-25 0.11675275904341476 1101 26-27 0.1346540433416834 1101 28-29 0.33043461230433024 1101 30-31 0.11595351096230644 1101 32-33 0.111922141426966 1101 34-35 0.1505653671750551 1101 36-37 0.1748746187178014 1101 38-39 0.15724733519819978 1101 40-41 0.10881216409713801 1101 42-43 -0.008873211694520933 1101 44-45 0.014467914353033962 1101 46-47 -0.021338147514960326 1101 48-49 -0.037334309334575266 1101 50-51 0.06252545217678218 1101 52-53 0.14702497585757257 1101 54-55 0.3128098855319621 1101 56-57 0.33869619728385914 1101 58-59 0.15397977967343124 1101 60-61 0.3366877353289617 1101 62-63 0.25678939164164305 1101 64-65 0.44829508852433264 1101 66-67 0.2506410916947921 1101 68-69 0.1261702492555763 1101 70-71 0.06131299562547454 1101 72-73 0.16628292837287617 1101 74-75 0.09537325456733114 1101 76-77 -0.13695192548409096 1101 78-79 -0.07721329294274426 1101 80-81 0.020015064830456453 1101 82-83 -0.06273847427896229 1101 84-85 -0.176393637463935 1101 86-87 -0.06746249322169717 1101 88-89 0.05695982493048746 1101 90-91 0.054545683806740186 1101 92-93 0.14237816362781786 1101 94-95 0.18834803176143566 1101 96-97 -0.013920080824171066 1101 98-99 0.017004133701316704 1101 100-101 0.09034186333362015 1101 102-103 0.0661850550206502 1101 104-105 0.05455975808026281 1101 106-107 0.20891259276577046 1101 108-109 0.21786552702707596 1101 110-111 -0.16283652447611274 1101 112-113 -0.33150538249538997 1101 114-115 -0.35055074342591297 1101 116-117 -0.192819434674405 1101 118-119 -0.016477741668293078 1101 120-121 0.05974928608236851 1101 122-123 -0.2572780746443506 1101 124-125 -0.10752033444385134 1102 1 -0.6178628236805288 1102 2 -0.6598702207532483 1102 3 -0.3742242080273037 1102 4 -0.4608006861044096 1102 5 -0.20501251064482062 1102 6 0.11973435741007421 1102 7 0.05518585704790269 1102 8 -0.05144984496491389 1102 9 -0.17072109711233452 1102 10-11 -0.0097789323614208 1102 12-13 0.010552900254985786 1102 14-15 0.11990469690226035 1102 16-17 -0.05484753323560909 1102 18-19 -0.01684201097982907 1102 20-21 -0.010902030644594163 1102 22-23 -0.04000957414960027 1102 24-25 -0.14965355494269517 1102 26-27 -0.22339236726976708 1102 28-29 -0.09801734915730265 1102 30-31 -0.34918823286106004 1102 32-33 -0.28641004045761775 1102 34-35 -0.334375802947406 1102 36-37 -0.33693539127426675 1102 38-39 -0.23247384076340438 1102 40-41 -0.29886727642403343 1102 42-43 -0.2487457140383711 1102 44-45 -0.3980510797336194 1102 46-47 -0.42072532625353176 1102 48-49 -0.418496318656004 1102 50-51 -0.3959294095867385 1102 52-53 -0.5071438949421712 1102 54-55 -0.604307387837391 1102 56-57 -0.6975383051312924 1102 58-59 -0.5811129095423624 1102 60-61 -0.5651345407238892 1102 62-63 -0.5536164290040695 1102 64-65 -0.6427234184850512 1102 66-67 -0.5539855161179084 1102 68-69 -0.7743234765531568 1102 70-71 -0.7403455723577004 1102 72-73 -0.7588241467256722 1102 74-75 -0.8882568056645184 1102 76-77 -0.9216382351920096 1102 78-79 -0.7475334727807308 1102 80-81 -0.8807689802331069 1102 82-83 -0.7274894000148571 1102 84-85 -0.6769756249512042 1102 86-87 -0.6555972715027707 1102 88-89 -0.6461036068878485 1102 90-91 -0.8579317810403495 1102 92-93 -0.5329784241525672 1102 94-95 -0.43977044316154945 1102 96-97 -0.4781899996831669 1102 98-99 -0.5503426103966653 1102 100-101 -0.6251585640032182 1102 102-103 -0.7565307645362545 1102 104-105 -0.7579385195643873 1102 106-107 -0.7201820601760147 1102 108-109 -0.5979294592085331 1102 110-111 -0.6751796696945185 1102 112-113 -0.7426220179910423 1102 114-115 -0.7125184392661481 1102 116-117 -0.5347823705507508 1102 118-119 -0.6271990303658832 1102 120-121 -0.7959444112051237 1102 122-123 -0.8858695589340613 1102 124-125 -0.9832483728473171 1103 1 -0.6909810598297774 1103 2 -0.5658504839617464 1103 3 -0.28719753336131504 1103 4 -0.10553209344686465 1103 5 -0.25456427934131654 1103 6 0.013850624495169939 1103 7 0.036538781031254075 1103 8 -0.04852182348980705 1103 9 0.004943077457511436 1103 10-11 0.019879904198560894 1103 12-13 0.11995431628901798 1103 14-15 -0.029394208574963443 1103 16-17 -0.15462591737232856 1103 18-19 -0.11201145818045433 1103 20-21 -0.14648132778098244 1103 22-23 -0.12255547411191259 1103 24-25 -0.13209052181472458 1103 26-27 -0.13313817723968668 1103 28-29 0.013256216156399603 1103 30-31 -0.14105315198329293 1103 32-33 -0.10118071370833803 1103 34-35 -0.07212193596691918 1103 36-37 -0.12693373756804505 1103 38-39 -0.14772130059373012 1103 40-41 -0.24699945370133491 1103 42-43 -0.170013722129994 1103 44-45 -0.07684008604847037 1103 46-47 -0.2480022489754532 1103 48-49 -0.3551418717245909 1103 50-51 -0.14676828091301175 1103 52-53 -0.25268417614914185 1103 54-55 -0.16455286706607097 1103 56-57 -0.16113032811396266 1103 58-59 -0.12162621226289971 1103 60-61 -0.050228686603855266 1103 62-63 -0.21242385683710197 1103 64-65 -0.20008529555699184 1103 66-67 -0.1776221699395073 1103 68-69 -0.12470767481330469 1103 70-71 -0.10599665188174257 1103 72-73 -0.2989770953223285 1103 74-75 -0.40618278885055403 1103 76-77 -0.45226497062208537 1103 78-79 -0.32359703663301786 1103 80-81 -0.42654116489440597 1103 82-83 -0.35139593484652565 1103 84-85 -0.3537323032322739 1103 86-87 -0.37787480955724106 1103 88-89 -0.2900953652775229 1103 90-91 -0.2832942503698668 1103 92-93 -0.3052663459437994 1103 94-95 -0.5027629014919981 1103 96-97 -0.2808917903008812 1103 98-99 -0.30184969441263476 1103 100-101 -0.2862153207391849 1103 102-103 -0.34867931350518333 1103 104-105 -0.5171277420147966 1103 106-107 -0.3751801949492233 1103 108-109 -0.3814061457965927 1103 110-111 -0.45307730912502464 1103 112-113 -0.5886684102193875 1103 114-115 -0.512935423213662 1103 116-117 -0.32373724744578425 1103 118-119 -0.5171072881286882 1103 120-121 -0.1901547087953297 1103 122-123 -0.3778171633675349 1103 124-125 -0.34475364932391983 1104 1 -0.09621556131826381 1104 2 -0.15156253135447528 1104 3 -0.01783922284642614 1104 4 -0.24497187886644056 1104 5 -0.08614755609485414 1104 6 0.14998049191191853 1104 7 0.09114692553729498 1104 8 0.22624016831793625 1104 9 0.1769302966414159 1104 10-11 0.1525166842983836 1104 12-13 0.1652257687358727 1104 14-15 0.23004740480721608 1104 16-17 0.27253546188141087 1104 18-19 0.21147692866120593 1104 20-21 0.14225394592843799 1104 22-23 0.10768294443928994 1104 24-25 8.089812778848682E-4 1104 26-27 0.09444493910080354 1104 28-29 0.2583463645571129 1104 30-31 0.11583864009156031 1104 32-33 0.17457714466559793 1104 34-35 0.20112107487401687 1104 36-37 0.2977536435211263 1104 38-39 0.2291401435713425 1104 40-41 0.28649842735425324 1104 42-43 0.09883052053390173 1104 44-45 0.10532021134984859 1104 46-47 -0.07787041183573251 1104 48-49 0.04813111397253067 1104 50-51 -0.13552080835109948 1104 52-53 -0.12661248644020162 1104 54-55 0.002694706220307097 1104 56-57 -0.06322884783730487 1104 58-59 0.1854299099196126 1104 60-61 0.19314644116700208 1104 62-63 0.015478966432493024 1104 64-65 -0.017741735581164875 1104 66-67 0.01634835136558266 1104 68-69 0.03416242046458251 1104 70-71 0.11494984104143313 1104 72-73 0.0023918248367387207 1104 74-75 0.03158758174088305 1104 76-77 0.06615856085244332 1104 78-79 0.06752578007082732 1104 80-81 0.026704867680635402 1104 82-83 -0.1712036816774365 1104 84-85 -0.07797491117532829 1104 86-87 -0.11650693394823364 1104 88-89 -0.06453084192809655 1104 90-91 0.02273056515513261 1104 92-93 0.006503308384743889 1104 94-95 0.10836594002737598 1104 96-97 0.17960882896808172 1104 98-99 0.1380842937541047 1104 100-101 -0.07675132414143704 1104 102-103 0.038625486202342074 1104 104-105 0.12152695211856468 1104 106-107 0.15710190444717398 1104 108-109 0.18991507946062924 1104 110-111 -0.016779567591477473 1104 112-113 -0.1717313107856846 1104 114-115 0.05092678198073486 1104 116-117 0.1336181569816155 1104 118-119 0.050109041919288444 1104 120-121 0.338738478499959 1104 122-123 -0.14330299106153888 1104 124-125 -0.16839899846477735 1105 1 0.09051107927434288 1105 2 0.16375397409935744 1105 3 -0.0015069778268497203 1105 4 0.1641256224393608 1105 5 0.03493186687747851 1105 6 0.14116048369123035 1105 7 0.07871753280919336 1105 8 0.11013049213764958 1105 9 0.10626181831226233 1105 10-11 0.0811574348579498 1105 12-13 0.053856090868372064 1105 14-15 0.09132231142701386 1105 16-17 0.07084492590333724 1105 18-19 -0.019263328118618972 1105 20-21 0.03795693140222767 1105 22-23 0.15461266114864713 1105 24-25 -0.16197284202758766 1105 26-27 0.08596677148852194 1105 28-29 0.11769766613609534 1105 30-31 0.05867829539704417 1105 32-33 0.21338947067150826 1105 34-35 0.05039314912585979 1105 36-37 -0.07369526167352092 1105 38-39 0.03970677870742634 1105 40-41 -0.044551630890580896 1105 42-43 -0.12877833626996704 1105 44-45 0.07472767566255811 1105 46-47 -0.07896378125872161 1105 48-49 -0.20164156067669836 1105 50-51 -0.04695896203488559 1105 52-53 -0.22804293996010827 1105 54-55 -0.07695236062752642 1105 56-57 -0.19794770211036194 1105 58-59 -0.10839901002919916 1105 60-61 -0.12510689601940328 1105 62-63 0.00387289891874687 1105 64-65 -0.03889932688374742 1105 66-67 -0.00713616125353056 1105 68-69 -0.048095701951869074 1105 70-71 0.10145714363274294 1105 72-73 0.013096309060379951 1105 74-75 -0.004325336405837987 1105 76-77 -0.08716830482187632 1105 78-79 -0.04378681965885889 1105 80-81 -0.15583110680292478 1105 82-83 -0.10942458484024442 1105 84-85 -0.139185739508342 1105 86-87 -0.1267361260053974 1105 88-89 -0.006299025712522166 1105 90-91 -0.09022769780277073 1105 92-93 0.04743093581886626 1105 94-95 -0.024360833388573866 1105 96-97 -0.04152856959706952 1105 98-99 -0.11760584016412778 1105 100-101 -0.059042293410779934 1105 102-103 -0.022056023173369965 1105 104-105 -0.042588399567126345 1105 106-107 -0.10708228203679226 1105 108-109 -0.009136759334914757 1105 110-111 0.044010085111388264 1105 112-113 -0.018211133304983917 1105 114-115 -0.039515232554443 1105 116-117 -0.07281049265375117 1105 118-119 0.014970986417079502 1105 120-121 0.14490776364115376 1105 122-123 -0.14449400358821762 1105 124-125 -0.14966875369290378 1106 1 0.29097938707530346 1106 2 0.19967825633879954 1106 3 0.28261838193193256 1106 4 0.18122588521968552 1106 5 0.13836429564995711 1106 6 0.412766494898392 1106 7 0.2784662373347402 1106 8 0.2972683121065529 1106 9 0.1745192230436814 1106 10-11 0.17171594162116577 1106 12-13 0.08816906515429679 1106 14-15 0.19605415721456154 1106 16-17 0.22960512728254656 1106 18-19 0.18089360299111235 1106 20-21 0.23990987092503957 1106 22-23 0.27196919045988466 1106 24-25 0.19536195176785043 1106 26-27 0.22949702722365828 1106 28-29 0.11074495802453299 1106 30-31 0.13141822172256212 1106 32-33 0.24241212668983536 1106 34-35 0.301144721436728 1106 36-37 0.23396616481397814 1106 38-39 0.3234207406908354 1106 40-41 0.5100968211395909 1106 42-43 0.32526124705265147 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-0.23616924084329582 2315 28-29 -0.07868998357194812 2315 30-31 -0.0038366221303434145 2315 32-33 0.023946995498540957 2315 34-35 -0.04621542205916285 2315 36-37 0.004816395308587573 2315 38-39 -0.11189259333571044 2315 40-41 -0.02755222048809003 2315 42-43 0.09646371837080636 2315 44-45 0.07863074014889548 2315 46-47 -0.14193421614016444 2315 48-49 0.0012804987945145285 2315 50-51 0.032597454632966816 2315 52-53 0.007573899797232997 2315 54-55 0.1254186382785214 2315 56-57 0.18000827697668598 2315 58-59 0.17367438420553327 2315 60-61 0.3428447498811167 2315 62-63 0.20148094443851505 2315 64-65 0.1474040743124938 2315 66-67 0.42244795939375024 2315 68-69 0.32175633038364637 2315 70-71 0.25793472472016177 2315 72-73 0.40362581024667676 2315 74-75 0.3318974391066867 2315 76-77 0.21261130646998083 2315 78-79 0.4310410960073874 2315 80-81 0.292114714158334 2315 82-83 0.33384885343670945 2315 84-85 0.4657503637493612 2315 86-87 0.3441679471899377 2315 88-89 0.3703605492089821 2315 90-91 0.21887163872243676 2315 92-93 0.11368038529106883 2315 94-95 -0.05072472160657071 2315 96-97 0.08800991051999674 2315 98-99 0.1342838908941637 2315 100-101 0.14223208964290635 2315 102-103 0.28271279067009303 2315 104-105 0.04286310426170559 2315 106-107 0.1087587113284556 2315 108-109 0.028287942669717125 2315 110-111 0.12363753762392449 2315 112-113 0.12287635444133471 2315 114-115 0.1828280268542528 2315 116-117 0.29522941528379576 2315 118-119 0.1321463881247844 2315 120-121 0.17332636366672816 2315 122-123 0.257909671806388 2315 124-125 0.2502804556781477 2316 1 -0.45431641126840105 2316 2 -0.27712633836162937 2316 3 0.0037978044036108827 2316 4 0.2632517304096105 2316 5 0.04204962828712411 2316 6 -0.42922686251070985 2316 7 -0.43698141237736365 2316 8 -0.45523861330208604 2316 9 -0.5446819256314228 2316 10-11 -0.4285161139561495 2316 12-13 -0.4391206707374522 2316 14-15 -0.3315474033591954 2316 16-17 -0.308112606114598 2316 18-19 -0.2293966115152628 2316 20-21 -0.22632169153416726 2316 22-23 -0.24520582295582471 2316 24-25 -0.24569883228992495 2316 26-27 -0.18943435684282406 2316 28-29 -0.12781397684037188 2316 30-31 -0.10930753209547817 2316 32-33 -0.13332501235496608 2316 34-35 -0.40366172970831826 2316 36-37 -0.4826620676476949 2316 38-39 -0.38813272636451757 2316 40-41 -0.2569512868822841 2316 42-43 -0.3925388660744389 2316 44-45 -0.242061851108069 2316 46-47 -0.23504436559723274 2316 48-49 -0.48756932289879984 2316 50-51 -0.45130335475919026 2316 52-53 -0.359701313569758 2316 54-55 -0.34181149755510987 2316 56-57 -0.24500123939146334 2316 58-59 -0.11516642198110816 2316 60-61 -0.2599195046366525 2316 62-63 -0.3071163428957 2316 64-65 -0.26693709249441255 2316 66-67 -0.17498813068118224 2316 68-69 -0.3753176368399771 2316 70-71 -0.165858699970304 2316 72-73 -0.05227834490017358 2316 74-75 -0.28913824226527396 2316 76-77 -0.17574717010973373 2316 78-79 -0.21544636242111181 2316 80-81 -0.23174983286254403 2316 82-83 -0.4802018137107851 2316 84-85 -0.5434058849984851 2316 86-87 -0.7000511095877222 2316 88-89 -0.5984903743795869 2316 90-91 -0.636634832407907 2316 92-93 -0.5084156198377556 2316 94-95 -0.6695561115973518 2316 96-97 -0.6260051954622909 2316 98-99 -0.6271512775956403 2316 100-101 -0.8032040605353963 2316 102-103 -0.7374053124673026 2316 104-105 -0.8383355574364124 2316 106-107 -0.7788359514915939 2316 108-109 -0.9986559007409461 2316 110-111 -0.7858492797018748 2316 112-113 -0.8132027015349479 2316 114-115 -0.7781157965219521 2316 116-117 -0.7559055368338434 2316 118-119 -1.3292061382205311 2316 120-121 -1.0696876203858423 2316 122-123 -1.1473618952890163 2316 124-125 -1.1334746030228189 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 6.0 10 12.0 11 28.0 12 63.0 13 181.0 14 503.0 15 3041.0 16 6256.0 17 8476.0 18 13154.0 19 22351.0 20 37441.0 21 64570.0 22 104271.0 23 152366.0 24 156491.0 25 103252.0 26 65629.0 27 54243.0 28 47421.0 29 47378.0 30 52974.0 31 61942.0 32 77081.0 33 98825.0 34 131630.0 35 176690.0 36 273688.0 37 533420.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.82247889896562 15.388846639768868 10.479969406010357 21.308705055255157 2 14.578295180728146 23.81694075088932 37.41740191354596 24.187362154836585 3 20.995461656931262 34.13420030453294 25.695664155636216 19.174673882899587 4 12.957551180587352 18.45256938539691 34.496914993350345 34.092964440665405 5 13.172538993187121 39.34702246727982 29.981767493459888 17.498671046073163 6 27.38974371496444 33.86633027874966 21.21053430214914 17.53339170413677 7 25.694955576562844 35.06948683691872 21.473944180302993 17.761613406215442 8 29.141914306544393 28.228111820785358 22.41177229805388 20.218201574616373 9 32.42958876489938 14.474941832680441 21.11107429021917 31.984395112201007 10-11 24.00546528623205 27.191957386987966 26.001271483536993 22.801305843242996 12-13 26.963713883065378 25.622595256616425 28.98088588740481 18.432804972913388 14-15 25.695129991314147 23.206754734488424 30.70290452885343 20.395210745344 16-17 19.58126070470536 27.565662793496422 30.507232979736493 22.345843522061724 18-19 19.9805657049881 25.435990770781057 36.011024830941906 18.572418693288935 20-21 22.034304042864356 24.881746282987425 35.135001966534894 17.948947707613325 22-23 20.55141767858307 27.372879194219706 32.58414056635633 19.49156256084089 24-25 19.353738767650835 28.622803238544396 33.280063975553944 18.74339401825082 26-27 20.19468014388549 27.759700159351937 33.43737320685468 18.60824648990789 28-29 19.703133012177638 28.613742835914092 33.67802775287523 18.005096399033043 30-31 20.938413916007047 28.614425390878615 32.837117956674035 17.610042736440302 32-33 17.97169597415871 29.52344657501753 33.45087434114828 19.053983109675485 34-35 17.311732357075833 30.788825595713583 32.771943119861305 19.12749892734928 36-37 18.952626337325107 31.063070990292076 31.402939935047947 18.58136273733487 38-39 18.833501062185835 30.29669257307106 31.36642184649409 19.503384518249014 40-41 18.002675163818356 31.063121438701792 31.180004513112554 19.754198884367298 42-43 18.83183843414985 30.587781868963194 30.938259195384333 19.642120501502625 44-45 19.14578150021706 29.836027015955747 31.03713447953103 19.981057004296165 46-47 18.093183313217022 29.36414885911971 31.907286711143357 20.635381116519913 48-49 18.037629025106714 29.51551799377164 31.393427426301663 21.05342555481998 50-51 17.034196389546597 28.94944040224307 31.396837425957457 22.61952578225288 52-53 17.38916361706007 27.94086073460792 30.99367398849188 23.676301659840124 54-55 17.85389656464005 27.224578749620903 31.136066807934405 23.785457877804646 56-57 18.561971085400597 25.817493728406376 31.343493099989743 24.27704208620328 58-59 17.97997259389172 25.830158657621094 32.15332989463276 24.036538853854424 60-61 18.554047899286996 25.306184296347347 31.782009768947916 24.35775803541774 62-63 17.605131517436902 25.419408754243506 32.706142523978556 24.269317204341043 64-65 17.12024834810721 25.51107209897536 32.71537148125406 24.653308071663368 66-67 17.64983630363325 24.874805631067538 32.538463725453745 24.936894339845466 68-69 17.97434226942826 24.576152757557608 32.72326186163214 24.726243111382 70-71 17.478950061892284 24.54667728093849 32.528879421580505 25.445493235588717 72-73 18.00303127727001 24.09565801243567 32.33194017054762 25.569370539746693 74-75 18.08302811507439 23.522365247943085 32.268536076557616 26.126070560424903 76-77 17.50776332143092 23.67590508660212 31.456635078293687 27.359696513673263 78-79 18.009931207633535 23.60968013900629 30.85613745788641 27.524251195473763 80-81 17.50505995005149 23.812802232559118 29.695627072146536 28.98651074524286 82-83 17.570850344361716 23.53769792883403 28.506966880231623 30.38448484657263 84-85 18.324885561330518 23.036689813978153 28.069515974317373 30.568908650373956 86-87 18.355336547789598 22.289483262067243 27.771504979574964 31.583675210568202 88-89 18.55115562066779 22.193339601600595 27.24566505586775 32.00983972186386 90-91 19.181019539094542 22.12568602696356 26.875380217566214 31.817914216375687 92-93 18.66996472321789 22.566099507713563 26.242984520067093 32.52095124900145 94-95 18.401763159105315 22.496172140420477 26.930973384957202 32.17109131551701 96-97 18.79294963250812 22.381336923951682 26.529159530196427 32.29655391334377 98-99 19.081723375467597 22.579612738038087 26.432109246471263 31.90655464002306 100-101 19.02010304423507 22.310415525703505 26.764227011263703 31.905254418797725 102-103 18.68254509493395 23.242596965880985 27.14922577291897 30.92563216626609 104-105 18.607960986908427 23.21464700198484 27.791028454022527 30.386363557084206 106-107 17.806743223114836 23.80724292137732 28.308626030355143 30.0773878251527 108-109 17.7504290602882 24.43624792010409 28.512647685690666 29.30067533391704 110-111 17.496350371329005 24.90385386834477 29.019562358506036 28.580233401820195 112-113 16.83798751823953 25.445691319185126 29.041047520450782 28.675273642124566 114-115 17.052721212149013 25.32666363895921 29.591515079228287 28.02910006966349 116-117 16.813544409748495 25.695319366805702 30.118820038687826 27.37231618475797 118-119 16.606803383674983 26.620179535252436 30.110208569690844 26.66280851138174 120-121 16.682817034783678 27.502428015112113 30.062419698600717 25.75233525150349 122-123 16.257217970423437 28.508119786809054 29.702214431726293 25.532447811041216 124-125 15.559403358896857 29.79292064261707 30.060975716107112 24.58670028237896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 43.0 1 28.5 2 21.0 3 42.5 4 77.0 5 180.0 6 392.5 7 754.5 8 1477.5 9 2165.0 10 2482.5 11 2807.5 12 3274.5 13 3838.5 14 4525.0 15 5311.5 16 6061.0 17 6902.5 18 7999.0 19 9303.0 20 10787.5 21 12320.5 22 14055.5 23 16189.5 24 18848.0 25 22055.5 26 25284.0 27 29177.0 28 33867.0 29 38596.5 30 43785.0 31 49086.0 32 53868.0 33 57046.5 34 58463.0 35 58266.5 36 56995.0 37 55015.0 38 51817.5 39 47959.5 40 44466.5 41 41525.0 42 39214.0 43 39688.5 44 42278.0 45 45596.0 46 51848.0 47 57764.0 48 75170.0 49 94893.5 50 116555.5 51 128452.0 52 117902.5 53 107801.5 54 96966.0 55 84179.0 56 69711.5 57 54595.5 58 41896.0 59 32861.0 60 24796.5 61 19240.0 62 14949.0 63 10163.0 64 6634.5 65 4779.5 66 3662.0 67 2721.5 68 2135.0 69 1766.5 70 1503.5 71 1265.5 72 1053.0 73 996.0 74 837.5 75 652.5 76 539.0 77 372.0 78 237.0 79 174.0 80 122.0 81 97.0 82 73.5 83 38.0 84 23.5 85 14.5 86 12.5 87 7.0 88 2.5 89 3.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11938689726622319 2 0.0021801843912750765 3 0.0015697327617180553 4 0.001482525386067052 5 0.009898037136388847 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.758571612952738E-4 20-21 4.360368782550153E-4 22-23 1.962165952147569E-4 24-25 3.488295026040122E-4 26-27 0.0014171198543287996 28-29 0.0 30-31 0.0010900921956375383 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.002899645240395852 42-43 0.27110592905505576 44-45 0.2614041085138817 46-47 0.2733297171341563 48-49 0.2801972979666728 50-51 0.2881113673070014 52-53 0.2824210860457734 54-55 0.2925589434652025 56-57 0.29146885126956495 58-59 0.2907275885765314 60-61 0.2898991185078469 62-63 0.2900953351030617 64-65 0.30156310500116856 66-67 0.3112867273862554 68-69 0.3093463632780206 70-71 0.31194078270363795 72-73 0.31477502241229555 74-75 0.31896097644354365 76-77 0.3118753771718997 78-79 0.3120497919232017 80-81 0.5334911205450112 82-83 0.4974526725572342 84-85 0.4722061373062688 86-87 0.4428826572436191 88-89 0.2936272338169273 90-91 0.012601465781569943 92-93 0.0843077304106072 94-95 2.1801843912750764E-5 96-97 0.0 98-99 4.360368782550153E-5 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.2217247525926753 114-115 0.19724128187865617 116-117 0.18714702814705256 118-119 0.16837564053817416 120-121 0.17277961300854983 122-123 0.16131184311044292 124-125 5.232442539060184E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2293384.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.37244017929242 #Duplication Level Percentage of deduplicated Percentage of total 1 71.68986674406335 26.075353896098896 2 15.1846142112215 11.04602944086575 3 5.930861361609359 6.471597002604396 4 2.5711006140953 3.7406881312449327 5 1.2630090664531646 2.2969360857735848 6 0.6734518893831117 1.4697053136131213 7 0.4322098816667997 1.1004369646077308 8 0.3001524964525028 0.8733822977507151 9 0.21038225203202018 0.6886904289137529 >10 1.4081327521253029 9.920146683317451 >50 0.16458811992293526 4.208588658149133 >100 0.13692000049635822 10.28890369387663 >500 0.01771687409832722 4.450609374151724 >1k 0.015547460943430007 10.319218683548467 >5k 8.436606713489151E-4 2.2879041215683924 >10k+ 6.026147652492251E-4 4.761809223915361 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43181 1.8828508439929816 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18357 0.8004328974127315 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17176 0.7489369420908143 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 15961 0.6959584613828299 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 13950 0.6082714451657464 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8710 0.3797881209601183 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 8009 0.3492219357944418 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 7748 0.3378413732719859 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7247 0.31599592567140955 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 7236 0.3155162851053291 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 7039 0.30692635860370526 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6202 0.2704300718937605 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 4742 0.20676868766852824 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 4724 0.20598382128766923 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 4279 0.18658018020532102 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 4258 0.1856645027609855 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 4238 0.18479242900447548 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 4201 0.18317909255493192 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 4184 0.18243782986189838 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 3867 0.1686154608212144 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3815 0.16634806905428834 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 3778 0.16473473260474478 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3626 0.15810697205526855 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 3455 0.15065074143710777 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 3201 0.1395754047294304 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 3143 0.1370463908355513 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 2982 0.13002619709564556 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 2979 0.12989538603216907 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2971 0.12954655652956507 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 2952 0.1287180864608805 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 2644 0.11528815061062606 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2518 0.10979408594461286 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 2512 0.10953246381765983 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 2353 0.1025994774534051 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.360368782550153E-5 0.0 3 0.0 0.0 0.0 4.360368782550153E-5 0.0 4 0.0 0.0 0.0 4.360368782550153E-5 0.0 5 0.0 0.0 0.0 4.360368782550153E-5 0.0 6 0.0 0.0 0.0 1.308110634765046E-4 0.0 7 0.0 0.0 0.0 1.308110634765046E-4 4.360368782550153E-5 8 0.0 0.0 0.0 1.308110634765046E-4 4.360368782550153E-5 9 0.0 0.0 0.0 1.308110634765046E-4 4.360368782550153E-5 10-11 4.360368782550153E-5 0.0 0.0 3.052258147785107E-4 4.360368782550153E-5 12-13 4.360368782550153E-5 0.0 0.0 5.886497856442707E-4 4.360368782550153E-5 14-15 4.360368782550153E-5 0.0 0.0 0.001024686663899286 4.360368782550153E-5 16-17 4.360368782550153E-5 0.0 0.0 0.0021147788595368244 4.360368782550153E-5 18-19 4.360368782550153E-5 0.0 0.0 0.004011539279946141 1.308110634765046E-4 20-21 4.360368782550153E-5 0.0 0.0 0.009941640824214348 1.308110634765046E-4 22-23 4.360368782550153E-5 0.0 0.0 0.029781318784817545 1.308110634765046E-4 24-25 4.360368782550153E-5 0.0 0.0 0.07604483156767466 1.308110634765046E-4 26-27 4.360368782550153E-5 0.0 0.0 0.14188640018418197 1.308110634765046E-4 28-29 4.360368782550153E-5 0.0 0.0 0.29875066713642373 2.1801843912750766E-4 30-31 4.360368782550153E-5 0.0 0.0 0.6298334687954569 2.1801843912750766E-4 32-33 4.360368782550153E-5 0.0 0.0 1.1451854552050595 2.1801843912750766E-4 34-35 4.360368782550153E-5 0.0 0.0 1.5685772639906792 2.1801843912750766E-4 36-37 4.360368782550153E-5 0.0 0.0 2.1021773937552544 2.1801843912750766E-4 38-39 4.360368782550153E-5 0.0 0.0 2.725208687249933 2.616221269530092E-4 40-41 4.360368782550153E-5 0.0 0.0 3.6446142468945455 3.052258147785107E-4 42-43 8.720737565100305E-5 0.0 0.0 4.441013803183418 3.052258147785107E-4 44-45 8.720737565100305E-5 0.0 0.0 5.106449683088397 3.052258147785107E-4 46-47 8.720737565100305E-5 0.0 0.0 5.7707518671099125 3.488295026040122E-4 48-49 8.720737565100305E-5 0.0 0.0 6.3520980350434115 3.488295026040122E-4 50-51 8.720737565100305E-5 0.0 0.0 6.9229139123670524 4.142350343422645E-4 52-53 8.720737565100305E-5 0.0 0.0 7.584687082494689 5.014424099932676E-4 54-55 8.720737565100305E-5 0.0 0.0 8.187464462994424 5.232442539060184E-4 56-57 8.720737565100305E-5 0.0 0.0 8.757124842590688 5.232442539060184E-4 58-59 8.720737565100305E-5 0.0 0.0 9.363237905209072 5.232442539060184E-4 60-61 8.720737565100305E-5 0.0 0.0 9.916721316622075 5.232442539060184E-4 62-63 8.720737565100305E-5 0.0 0.0 10.462901110324307 5.232442539060184E-4 64-65 8.720737565100305E-5 0.0 0.0 11.037641319552243 5.232442539060184E-4 66-67 8.720737565100305E-5 0.0 0.0 11.584148140913166 5.232442539060184E-4 68-69 8.720737565100305E-5 0.0 0.0 12.185573807090309 5.232442539060184E-4 70-71 8.720737565100305E-5 0.0 0.0 12.860689705692549 5.450460978187691E-4 72-73 8.720737565100305E-5 0.0 0.0 13.458430860248436 6.54055317382523E-4 74-75 8.720737565100305E-5 0.0 0.0 14.02277158992999 6.54055317382523E-4 76-77 8.720737565100305E-5 0.0 0.0 14.58700331039198 6.54055317382523E-4 78-79 8.720737565100305E-5 0.0 0.0 15.124396960997373 6.54055317382523E-4 80-81 8.720737565100305E-5 0.0 0.0 15.681455874812068 6.976590052080244E-4 82-83 8.720737565100305E-5 0.0 0.0 16.323105943008237 6.976590052080244E-4 84-85 8.720737565100305E-5 0.0 0.0 16.89084776034018 6.976590052080244E-4 86-87 8.720737565100305E-5 0.0 0.0 17.43585025447112 6.976590052080244E-4 88-89 8.720737565100305E-5 0.0 0.0 17.974900845213885 6.976590052080244E-4 90-91 8.720737565100305E-5 0.0 0.0 18.503704569317655 6.976590052080244E-4 92-93 8.720737565100305E-5 0.0 0.0 19.013475283685594 6.976590052080244E-4 94-95 8.720737565100305E-5 0.0 0.0 19.524379693936993 6.976590052080244E-4 96-97 8.720737565100305E-5 0.0 0.0 20.03421581383667 6.976590052080244E-4 98-99 8.720737565100305E-5 0.0 0.0 20.568927837640796 7.194608491207752E-4 100-101 8.720737565100305E-5 0.0 0.0 21.15180013464819 7.41262693033526E-4 102-103 8.720737565100305E-5 0.0 0.0 21.67493537933464 7.848663808590275E-4 104-105 8.720737565100305E-5 0.0 0.0 22.164125153048943 7.848663808590275E-4 106-107 8.720737565100305E-5 0.0 0.0 22.642501212182523 8.284700686845291E-4 108-109 8.720737565100305E-5 0.0 0.0 23.144336055366217 9.156774443355321E-4 110-111 8.720737565100305E-5 0.0 0.0 23.62210166287024 9.810829760737844E-4 112-113 8.720737565100305E-5 0.0 0.0 24.1435363637315 0.0010028848199865352 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 945 0.0 73.61598 1 ATAACGC 635 0.0 64.53721 3 GTATAAC 665 0.0 63.4826 1 TACAACG 540 0.0 60.492813 2 ATAGTAC 1230 0.0 58.425926 4 GCATAGT 1220 0.0 57.44563 2 AGATTAC 1365 0.0 54.389137 2 GACCGTT 340 0.0 54.146484 7 TAGTACT 1385 0.0 52.313828 5 CATAGTA 1445 0.0 49.322857 3 GATTACT 1525 0.0 49.072197 3 TAACGCA 910 0.0 46.991188 4 ACAACGC 730 0.0 46.375313 3 GTACAAC 760 0.0 44.594296 1 TCACGCA 565 0.0 43.09836 4 ATAAACG 670 0.0 39.00432 3 CGCAAAG 675 0.0 38.758495 1 AGTGTAC 1045 0.0 38.648037 3 TTCAACG 1385 0.0 38.165028 4 ATTACTT 1995 0.0 37.510513 4 >>END_MODULE