FastQCFastQC Report
Fri 10 Feb 2017
SRR3557002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3557002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2664007
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT339271.273532689666356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144620.5428664414170082No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA143820.5398634463047582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134360.504353029102401No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT126160.4735723292018377No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77460.2907650017436141No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG68940.25878310379815067No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT64740.24301737945883775No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT64730.2429798420199346No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC61840.23213152217693123No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60870.22849039060332801No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA56530.2121991421193713No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA56320.21141085590240566No Hit
ACTTATTACTGTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGG55230.2073192750619649No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG54000.20270217007688046No Hit
GTCTACAGCATGGTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTG53550.20101298532623976No Hit
GTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATGGTTAGAGAAT49710.1865986087874394No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT45980.17259714407657337No Hit
GCTGTAGACAGTAATAAGTTGCTGTATCGTCAGACTCCAGGCTGCTGATG43790.16437644495678877No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC43550.16347554642311377No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA43390.16287494740066374No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG40990.15386596206391348No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC40660.15262722658011033No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT40220.1509755792683728No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT39440.14804765903392897No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC37780.14181644417601005No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG37640.14129092003136628No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT34500.1295041642157847No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC34430.12924140214346283No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA34100.12800266665965968No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA33300.1249996715474096No Hit
CCTAAGACCCTGATCTATTATGCAACAAGCTTGGCAGATGGGGTCCCATC32560.12222190106857826No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32290.12120839021819386No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT31810.11940659315084383No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG30690.11520239999369371No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA29820.11193664280912176No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG29180.10953424671932169No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA28940.10863334818564666No Hit
CTATTATGCAACAAGCTTGGCAGATGGGGTCCCATCAAGATTCAGTGGCA28850.10829551123551853No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA28680.10765737477416538No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT28470.10686908855719975No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT27740.10412885551727154No Hit
GTGAGAGCCCGTACACGTTCGGAGGGGGGACCAAGCTGGAAATAAAACGG27580.10352825649482152No Hit
GTACATGGGGTTGTCATTGCAGTCAGGACTCAGCATGGACATGAGGGCCC27010.10138862247734334No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA26980.10127601016063396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6900.077.5709841
TACAACG5350.073.276682
GTACAAC5550.071.793931
AGATTAC11500.057.848992
ATAACGC7650.055.9034353
TCACGCA4900.055.759784
ATAGTAC10000.055.2385444
GCATAGT9900.055.1986242
ACAACGC7150.054.8283733
AGTGTAC8300.052.956723
TAGTACT10800.052.79675
TACGCAG3600.051.14685
CATAGTA11050.049.9905743
TAACGCA8900.049.3856744
TATCACG5500.048.5987852
GATTACT14200.047.6852383
AAACGCA7750.047.517035
GACCGTT3250.047.5054327
GTATAAC9550.046.0823141
TCAAACG3100.045.9850853
Produced by FastQC (version 0.11.5)