##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3556999.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4558395 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.75780400777028 33.0 32.0 34.0 27.0 34.0 2 32.00028101996426 33.0 32.0 34.0 28.0 34.0 3 32.126861537887784 33.0 32.0 34.0 28.0 34.0 4 31.978239051245012 33.0 32.0 34.0 28.0 34.0 5 32.128913795316116 33.0 32.0 34.0 30.0 34.0 6 35.4933269275699 38.0 36.0 38.0 29.0 38.0 7 35.80687391066373 38.0 37.0 38.0 31.0 38.0 8 35.99023055263969 38.0 37.0 38.0 31.0 38.0 9 36.11192799219901 38.0 37.0 38.0 33.0 38.0 10-11 36.11162503468874 38.0 37.0 38.0 32.5 38.0 12-13 36.092865471289784 38.0 37.0 38.0 32.0 38.0 14-15 36.099875833489634 38.0 37.0 38.0 32.5 38.0 16-17 36.0430451946354 38.0 37.0 38.0 31.5 38.0 18-19 36.02116720907249 38.0 37.0 38.0 31.0 38.0 20-21 35.988732218247875 38.0 37.0 38.0 31.0 38.0 22-23 35.93397401936427 38.0 37.0 38.0 31.0 38.0 24-25 35.843667124064496 38.0 37.0 38.0 31.0 38.0 26-27 35.73916301680745 38.0 37.0 38.0 30.0 38.0 28-29 35.65773007824026 38.0 37.0 38.0 29.0 38.0 30-31 35.56472892322846 38.0 37.0 38.0 29.0 38.0 32-33 35.465054476411105 38.0 37.0 38.0 29.0 38.0 34-35 35.284559477623155 38.0 36.0 38.0 27.5 38.0 36-37 35.13430812818986 38.0 36.0 38.0 27.0 38.0 38-39 34.96820613395724 38.0 36.0 38.0 25.5 38.0 40-41 34.724237258947504 38.0 36.0 38.0 24.5 38.0 42-43 34.30876383025166 38.0 35.0 38.0 16.0 38.0 44-45 33.993166015669985 38.0 34.5 38.0 16.0 38.0 46-47 33.51297737471194 38.0 34.0 38.0 15.0 38.0 48-49 33.11406646418312 38.0 33.0 38.0 15.0 38.0 50-51 32.793519429536055 38.0 31.5 38.0 15.0 38.0 52-53 32.53636652813106 38.0 30.0 38.0 15.0 38.0 54-55 32.37528450254969 38.0 29.0 38.0 15.0 38.0 56-57 32.209643087095344 38.0 28.0 38.0 15.0 38.0 58-59 32.088355221519855 38.0 27.0 38.0 15.0 38.0 60-61 31.935990518592618 38.0 26.5 38.0 15.0 38.0 62-63 31.82934673278643 38.0 26.0 38.0 15.0 38.0 64-65 31.741031108537108 38.0 26.0 38.0 15.0 38.0 66-67 31.572465198825462 38.0 25.5 38.0 15.0 38.0 68-69 31.46523447397604 38.0 25.0 38.0 15.0 38.0 70-71 31.277700813553892 38.0 25.0 38.0 15.0 38.0 72-73 31.117823597998857 38.0 25.0 38.0 14.0 38.0 74-75 30.993008284714247 38.0 24.5 38.0 14.0 38.0 76-77 30.919239666593175 38.0 24.0 38.0 14.0 38.0 78-79 30.715822674428168 38.0 23.0 38.0 14.0 38.0 80-81 30.645481030055535 38.0 23.5 38.0 14.0 38.0 82-83 30.539781984667847 38.0 22.5 38.0 14.0 38.0 84-85 30.446612239615042 37.5 22.0 38.0 14.0 38.0 86-87 30.34746144202071 37.0 21.5 38.0 13.5 38.0 88-89 30.251042307654338 37.0 21.0 38.0 13.0 38.0 90-91 30.251441132240622 37.0 21.0 38.0 13.0 38.0 92-93 30.206574024409907 37.0 21.0 38.0 13.0 38.0 94-95 30.156102312327036 37.0 21.0 38.0 13.0 38.0 96-97 30.105506324046072 37.0 21.0 38.0 13.0 38.0 98-99 30.016966936827544 37.0 21.0 38.0 13.0 38.0 100-101 29.947844032822957 37.0 21.0 38.0 13.0 38.0 102-103 29.870450564288525 37.0 18.0 38.0 13.0 38.0 104-105 29.69491893528314 36.0 15.0 38.0 13.0 38.0 106-107 29.55749392055756 36.0 15.0 38.0 13.0 38.0 108-109 29.427244896504142 36.0 15.0 38.0 13.0 38.0 110-111 29.302374739354534 35.5 15.0 38.0 13.0 38.0 112-113 28.979117320899135 35.0 15.0 38.0 13.0 38.0 114-115 28.850289411075607 35.0 14.0 38.0 13.0 38.0 116-117 28.72397082745133 35.0 14.0 38.0 13.0 38.0 118-119 28.528379287007816 34.5 14.0 38.0 13.0 38.0 120-121 28.36245257376774 34.0 14.0 38.0 13.0 38.0 122-123 28.156075000082264 34.0 14.0 38.0 13.0 38.0 124-125 26.929018766473725 29.5 14.0 37.0 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.3413259752372433 1101 2 -0.592896741785065 1101 3 -0.2841804654107456 1101 4 -0.3079339339451046 1101 5 -0.16539907157429568 1101 6 -0.02635618603189016 1101 7 -0.005411194491365734 1101 8 -0.054111508919383766 1101 9 0.01013325836995449 1101 10-11 0.06452842660202407 1101 12-13 0.0237180539298123 1101 14-15 0.0999187266446313 1101 16-17 0.04698817175456327 1101 18-19 -0.016635930623174033 1101 20-21 0.064648193093511 1101 22-23 0.013281436697205606 1101 24-25 -0.001357244889980791 1101 26-27 -0.0019183348797042754 1101 28-29 0.04761282242028386 1101 30-31 -0.016960057519540328 1101 32-33 -0.020719265624762784 1101 34-35 -0.033028200193378154 1101 36-37 0.006338452482616219 1101 38-39 -0.07968716793337904 1101 40-41 -0.12212883788443207 1101 42-43 -0.16980487272710576 1101 44-45 -0.1389349850462338 1101 46-47 -0.2989041343109591 1101 48-49 -0.3232492398385034 1101 50-51 -0.2489719258627403 1101 52-53 -0.3759863551505944 1101 54-55 -0.2339417259800598 1101 56-57 -0.35055796307885245 1101 58-59 -0.3172123554977979 1101 60-61 -0.33770455883293593 1101 62-63 -0.33996864483248146 1101 64-65 -0.30016889245262135 1101 66-67 -0.23549866541781128 1101 68-69 -0.3891667790545448 1101 70-71 -0.37571471525342304 1101 72-73 -0.38476594979922396 1101 74-75 -0.5174888566186304 1101 76-77 -0.49064547589994945 1101 78-79 -0.3817122335841283 1101 80-81 -0.37765130208060427 1101 82-83 -0.3786421223371548 1101 84-85 -0.49601718753623913 1101 86-87 -0.4807451340543629 1101 88-89 -0.39406673165496997 1101 90-91 -0.3541378949839 1101 92-93 -0.2670741387327418 1101 94-95 -0.19446199186594626 1101 96-97 -0.48669295332244644 1101 98-99 -0.39805222505578897 1101 100-101 -0.43004255576534334 1101 102-103 -0.48183758204223537 1101 104-105 -0.641979005119115 1101 106-107 -0.3599667422168338 1101 108-109 -0.4234134481030729 1101 110-111 -0.45418651972579127 1101 112-113 -0.7991920528315042 1101 114-115 -0.6164317168061295 1101 116-117 -0.3719751619858904 1101 118-119 -0.4506023636018597 1101 120-121 -0.49844470659687445 1101 122-123 -0.5913394574914115 1101 124-125 -0.5771464635933548 1102 1 -0.7399273738386434 1102 2 -0.5996641679073313 1102 3 -0.3671942258438605 1102 4 -0.5321613194628121 1102 5 -0.25811280944932236 1102 6 -0.03131896518821975 1102 7 -0.0881993740537439 1102 8 -0.09020444823812568 1102 9 -0.1281478153949962 1102 10-11 -0.04363760091884217 1102 12-13 0.0063483268826658446 1102 14-15 -0.00632986347492448 1102 16-17 0.021497533360694376 1102 18-19 -0.033554495928839856 1102 20-21 -0.07904682156924281 1102 22-23 -0.006569679928098537 1102 24-25 -0.0808745018265924 1102 26-27 -0.07996931615649316 1102 28-29 -0.11435424277258477 1102 30-31 -0.2628432066284958 1102 32-33 -0.12358414268319251 1102 34-35 -0.16330115304698012 1102 36-37 -0.16092976317268892 1102 38-39 -0.09333481061327831 1102 40-41 -0.09257774381721617 1102 42-43 -0.17894089799215607 1102 44-45 -0.11851991624406821 1102 46-47 -0.271834429821908 1102 48-49 -0.2900888518394069 1102 50-51 -0.22539872487018187 1102 52-53 -0.3027853625947614 1102 54-55 -0.19311158837572862 1102 56-57 -0.3064569028487618 1102 58-59 -0.30311355107641447 1102 60-61 -0.25830009233169093 1102 62-63 -0.1527365221164878 1102 64-65 -0.11417746452571365 1102 66-67 -0.12879891355676776 1102 68-69 -0.20994277724989985 1102 70-71 -0.1420129331645441 1102 72-73 -0.239040707299786 1102 74-75 -0.24994994391842695 1102 76-77 -0.43571653387423837 1102 78-79 -0.32080539848374556 1102 80-81 -0.42592560841942984 1102 82-83 -0.38924442326203845 1102 84-85 -0.3148757483302873 1102 86-87 -0.3496826481531663 1102 88-89 -0.23131013341449957 1102 90-91 -0.4891480461230806 1102 92-93 -0.3918203602531598 1102 94-95 -0.20946311977030163 1102 96-97 -0.25806674082526726 1102 98-99 -0.2943978149497646 1102 100-101 -0.3217637378091034 1102 102-103 -0.3759273632287865 1102 104-105 -0.3459041122700306 1102 106-107 -0.39019457889627773 1102 108-109 -0.34491130508479984 1102 110-111 -0.47978994855502677 1102 112-113 -0.6540307625089277 1102 114-115 -0.3007312882024742 1102 116-117 -0.38178792986317234 1102 118-119 -0.37819090409362843 1102 120-121 -0.30985909865642824 1102 122-123 -0.5084384874936667 1102 124-125 -0.6456102578636056 1103 1 -0.6395479381028046 1103 2 -0.6201167659922113 1103 3 -0.20794922384787284 1103 4 -0.1756790703070692 1103 5 -0.20810425520391007 1103 6 -0.05617236547797688 1103 7 -0.10619008158049326 1103 8 -0.0014799059814194493 1103 9 -0.11502902665588266 1103 10-11 -0.010768533343750164 1103 12-13 0.014168877350634546 1103 14-15 -0.10543330359839587 1103 16-17 -0.029318254990762682 1103 18-19 -0.07580953075307661 1103 20-21 -0.10037244396023226 1103 22-23 -0.07065941350666094 1103 24-25 -0.14809028284438597 1103 26-27 -0.024217414191696207 1103 28-29 -0.04437978091768713 1103 30-31 -0.13046886717678774 1103 32-33 -0.050717469689047334 1103 34-35 -0.10769245492411983 1103 36-37 -0.12631213996851898 1103 38-39 -0.07453575475076235 1103 40-41 -0.09696408635917919 1103 42-43 3.2736912030628673E-4 1103 44-45 0.02445208994983261 1103 46-47 -0.19199409691437097 1103 48-49 -0.20105751655967907 1103 50-51 -0.2771764479944352 1103 52-53 -0.30978110451525964 1103 54-55 -0.28270905364493615 1103 56-57 -0.3216418533328991 1103 58-59 -0.32259489537633357 1103 60-61 -0.24360768202895855 1103 62-63 -0.30672684209388734 1103 64-65 -0.3311375399529872 1103 66-67 -0.349625112865521 1103 68-69 -0.4162039429712081 1103 70-71 -0.3924962809507875 1103 72-73 -0.46182438030942663 1103 74-75 -0.5635713718119142 1103 76-77 -0.7032214878914935 1103 78-79 -0.39401743204312467 1103 80-81 -0.43771177841786013 1103 82-83 -0.5665063678299198 1103 84-85 -0.527298097914592 1103 86-87 -0.5451467506598711 1103 88-89 -0.45292140885198506 1103 90-91 -0.48395647002387676 1103 92-93 -0.5030584457188318 1103 94-95 -0.4426861629322154 1103 96-97 -0.36162221239716175 1103 98-99 -0.4494191012955575 1103 100-101 -0.5123940838959449 1103 102-103 -0.5205617495125807 1103 104-105 -0.4923466401493215 1103 106-107 -0.529126818636783 1103 108-109 -0.5729084589369258 1103 110-111 -0.4973122744264984 1103 112-113 -0.6693493952356171 1103 114-115 -0.5335061577844158 1103 116-117 -0.5531976614037042 1103 118-119 -0.5768309158193752 1103 120-121 -0.3430965964165722 1103 122-123 -0.6203536700992061 1103 124-125 -0.9152655992630798 1104 1 -0.12746987044926428 1104 2 -0.039211793397956995 1104 3 -0.21569619812778384 1104 4 -0.36766340713092305 1104 5 -0.19917158502617127 1104 6 0.11441866688348767 1104 7 0.0330010626837165 1104 8 0.15220977172724304 1104 9 0.13573793622923347 1104 10-11 0.12816250370757132 1104 12-13 0.058871803233465414 1104 14-15 0.18809375121098526 1104 16-17 0.14486858790518653 1104 18-19 0.13269880726459604 1104 20-21 0.014928461838444207 1104 22-23 0.04196410233080172 1104 24-25 -0.015856141840316695 1104 26-27 0.02720461384654982 1104 28-29 0.1498059932779725 1104 30-31 -0.014021890601071618 1104 32-33 0.018919131110187948 1104 34-35 0.04152595022015504 1104 36-37 -0.030095955535301755 1104 38-39 0.028116892693532236 1104 40-41 0.007543548080761298 1104 42-43 -0.124589909367252 1104 44-45 -0.03800474473436566 1104 46-47 -0.11704853195539044 1104 48-49 -0.12032736067480698 1104 50-51 -0.1027036826582588 1104 52-53 -0.2052648232273242 1104 54-55 -0.26205860114660595 1104 56-57 -0.369153225316488 1104 58-59 -0.2957768341811118 1104 60-61 -0.1328922122514733 1104 62-63 -0.09801185694090364 1104 64-65 -0.15461642404943277 1104 66-67 -0.16303545678149334 1104 68-69 -0.11859705464647874 1104 70-71 -0.20950812477658332 1104 72-73 -0.2547894401260642 1104 74-75 -0.29765417884042833 1104 76-77 -0.3490526845761579 1104 78-79 -0.223063282192701 1104 80-81 -0.23366115370439644 1104 82-83 -0.26837492912374117 1104 84-85 -0.21826022692596325 1104 86-87 -0.22090674343361272 1104 88-89 -0.21327469349629524 1104 90-91 -0.25813165345986633 1104 92-93 -0.28981952995036053 1104 94-95 -0.16432304784933294 1104 96-97 -0.18257007636504952 1104 98-99 -0.3793007081516002 1104 100-101 -0.2480026711132659 1104 102-103 -0.3383846676799145 1104 104-105 -0.37334147479802837 1104 106-107 -0.16017398551333173 1104 108-109 -0.21291403602856818 1104 110-111 -0.4812044247226517 1104 112-113 -0.6517401018339761 1104 114-115 -0.5650105850207119 1104 116-117 -0.3813957445002636 1104 118-119 -0.31089973751114286 1104 120-121 -0.17183223776155998 1104 122-123 -0.3317263203746563 1104 124-125 -0.4125551006462267 1105 1 0.06225610960526495 1105 2 0.05751282470232155 1105 3 -0.057111467525572834 1105 4 -0.02050939718323974 1105 5 -0.15947990889796415 1105 6 0.03019525886900709 1105 7 -0.11572033707933826 1105 8 -0.045222916008256675 1105 9 -0.08895443373737777 1105 10-11 -0.04801094660064109 1105 12-13 -0.033349236006650074 1105 14-15 0.0971454515845025 1105 16-17 0.05550778192409922 1105 18-19 -0.015593859282610367 1105 20-21 0.03023970548378685 1105 22-23 6.748974073431668E-4 1105 24-25 -0.10359527718593853 1105 26-27 0.007557634611075059 1105 28-29 0.06338613285900152 1105 30-31 0.09147203540293702 1105 32-33 0.09161064308346312 1105 34-35 -0.0023220023625825092 1105 36-37 -0.039827488213084905 1105 38-39 -0.0017984877615120354 1105 40-41 0.014257254931465013 1105 42-43 -0.01879667723516576 1105 44-45 0.05770210798925035 1105 46-47 -0.07694148684430502 1105 48-49 -0.05399717569609663 1105 50-51 -0.13210073205596018 1105 52-53 -0.10692262998233559 1105 54-55 -0.1407040848813068 1105 56-57 -0.10463529738956368 1105 58-59 -0.13601308124774647 1105 60-61 -0.09720836900079988 1105 62-63 -0.049458233387202455 1105 64-65 -0.11340673391713807 1105 66-67 -0.13263168306698603 1105 68-69 -0.10445436707815148 1105 70-71 -0.03727192599362894 1105 72-73 -0.047440635226191574 1105 74-75 -0.17461687093151923 1105 76-77 -0.21113250698418184 1105 78-79 -0.1189807197816144 1105 80-81 -0.2614350236016243 1105 82-83 -0.1858201821122627 1105 84-85 -0.1588030178826827 1105 86-87 -0.05480051234322403 1105 88-89 0.019914215110027556 1105 90-91 0.07878248000207222 1105 92-93 0.014406480018017476 1105 94-95 -0.05192283112841878 1105 96-97 -0.029052162377158908 1105 98-99 -0.08499534194263703 1105 100-101 -0.03338707650956252 1105 102-103 -0.1734514474108657 1105 104-105 -0.29768887511222886 1105 106-107 -0.18527591892505768 1105 108-109 -0.1592812614878234 1105 110-111 -0.21215508775865288 1105 112-113 -0.2997204812239538 1105 114-115 -0.0699783673996528 1105 116-117 -0.11769315453586415 1105 118-119 -0.11477221801193593 1105 120-121 -0.01835285970709677 1105 122-123 -0.19364512533057265 1105 124-125 -0.14447352096131638 1106 1 0.3171003956481151 1106 2 0.18978673010565927 1106 3 0.038142211170018925 1106 4 0.1623544025985133 1106 5 0.02631228084675996 1106 6 0.30599610500199503 1106 7 0.17497050269449943 1106 8 0.1701136104028862 1106 9 0.17701796766004207 1106 10-11 0.07569999109931302 1106 12-13 0.05697513293420542 1106 14-15 0.1927440746449065 1106 16-17 0.103245576430119 1106 18-19 0.04741329133055672 1106 20-21 0.18981351913246414 1106 22-23 0.23806637711090417 1106 24-25 0.1656992231292307 1106 26-27 0.14119858749091918 1106 28-29 0.08359526110529458 1106 30-31 0.10598895298871014 1106 32-33 0.1718933007877439 1106 34-35 0.1492573372211794 1106 36-37 0.12355845010877431 1106 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-0.201483825036739 2315 20-21 -0.15994647767563208 2315 22-23 -0.20449787852679435 2315 24-25 -0.23372533868994338 2315 26-27 -0.21423083054912695 2315 28-29 -0.187963677366902 2315 30-31 -0.10089593837116695 2315 32-33 -0.13329297664889594 2315 34-35 -0.14439273523369422 2315 36-37 -0.21545547287981037 2315 38-39 -0.2131299748470994 2315 40-41 -0.20014934229993742 2315 42-43 -0.06754067637067607 2315 44-45 -0.037172484714112386 2315 46-47 -0.1162310374607074 2315 48-49 -0.1388032753135704 2315 50-51 -0.19790937744490833 2315 52-53 -0.11418855375656989 2315 54-55 -0.16687908587115174 2315 56-57 -0.16396611493593127 2315 58-59 -0.09844631932879366 2315 60-61 -0.026081517492240636 2315 62-63 -0.08240321986781751 2315 64-65 -0.14898204011848648 2315 66-67 0.06921290104597233 2315 68-69 -0.0776760645987693 2315 70-71 0.0873199778341629 2315 72-73 -0.012551782382789867 2315 74-75 -0.13269738347134918 2315 76-77 -0.14107561642172683 2315 78-79 0.12343731402984659 2315 80-81 -0.10378518458787767 2315 82-83 -0.12093264861784903 2315 84-85 -0.09134333944437856 2315 86-87 -0.10750881959775782 2315 88-89 -0.13551901400719757 2315 90-91 -0.2524256684486055 2315 92-93 -0.14112264851842227 2315 94-95 -0.3277198537239734 2315 96-97 -0.24120965021417007 2315 98-99 -0.09560740771116727 2315 100-101 -0.07955239927056823 2315 102-103 -0.038915012545874816 2315 104-105 -0.2025086565002887 2315 106-107 -0.29475537606677804 2315 108-109 -0.2788942557555245 2315 110-111 -0.14582840983300116 2315 112-113 -0.1666791809161161 2315 114-115 -0.053879652168088654 2315 116-117 -0.021375340887669125 2315 118-119 -0.10010048523735904 2315 120-121 -0.27864766686390396 2315 122-123 -0.1803206915594373 2315 124-125 -0.11047147874061736 2316 1 -0.27406870290982255 2316 2 -0.14377805548147293 2316 3 0.01115750654406611 2316 4 0.15212993420262322 2316 5 0.16858568372858684 2316 6 -0.2285339183037749 2316 7 -0.4226540310460507 2316 8 -0.2885252086029979 2316 9 -0.3093080947625211 2316 10-11 -0.3977604345633239 2316 12-13 -0.3039850858532631 2316 14-15 -0.22351443582780917 2316 16-17 -0.26756989667992315 2316 18-19 -0.04166804157409132 2316 20-21 -0.06224918905601129 2316 22-23 -0.30786372344065427 2316 24-25 -0.23617751125178188 2316 26-27 -0.29075384553777894 2316 28-29 -0.2182501677157873 2316 30-31 -0.03995204453826773 2316 32-33 -0.1308790682760801 2316 34-35 -0.0800312966941874 2316 36-37 -0.24250307800792825 2316 38-39 -0.13820969540518746 2316 40-41 -0.06866283941549511 2316 42-43 0.07320417465921025 2316 44-45 0.08421024363775587 2316 46-47 -0.07116160556243045 2316 48-49 -0.15130773713719492 2316 50-51 -0.17645900005880577 2316 52-53 -0.2147818655400897 2316 54-55 -0.14895799115252828 2316 56-57 -0.09304625036084957 2316 58-59 -0.040554303528416824 2316 60-61 0.023308713158971983 2316 62-63 0.053375797515268886 2316 64-65 -0.011265236529290235 2316 66-67 -0.09776120046376846 2316 68-69 -0.06335098753175572 2316 70-71 0.04685502666542263 2316 72-73 0.1297825131592667 2316 74-75 0.056136039572582064 2316 76-77 0.04268219369332016 2316 78-79 -0.062046978159134625 2316 80-81 0.06301902485532551 2316 82-83 -0.167218280132726 2316 84-85 -0.014817689566420711 2316 86-87 -0.25135281344451954 2316 88-89 -0.20172970180844274 2316 90-91 -0.15733248990188642 2316 92-93 -0.19684199988235562 2316 94-95 -0.2404441119513301 2316 96-97 -0.3094385000043083 2316 98-99 -0.326199256081825 2316 100-101 -0.3128646426078667 2316 102-103 -0.2817513081321401 2316 104-105 -0.3394265290057845 2316 106-107 -0.3496251971452011 2316 108-109 -0.5351437235312027 2316 110-111 -0.4583557322360967 2316 112-113 -0.5495461326234015 2316 114-115 -0.5853959829322299 2316 116-117 -0.5717846208092645 2316 118-119 -0.8054797306852706 2316 120-121 -0.7237677026275122 2316 122-123 -0.5735782709701063 2316 124-125 -0.7780444929224792 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 12.0 10 25.0 11 48.0 12 139.0 13 322.0 14 883.0 15 6124.0 16 12135.0 17 15047.0 18 21431.0 19 34486.0 20 56041.0 21 96876.0 22 159441.0 23 239746.0 24 249217.0 25 170093.0 26 115245.0 27 98910.0 28 90161.0 29 92530.0 30 105378.0 31 124347.0 32 153457.0 33 196174.0 34 265668.0 35 363076.0 36 585098.0 37 1306284.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.71683421886703 16.003325418807286 11.148104137884076 23.13173622444161 2 14.593792258004507 22.927564350631858 37.422416387835135 25.0562270035285 3 20.23583198232686 32.14493027051574 26.366169189154796 21.253068558002603 4 12.782119174352442 18.20741576982537 35.18316973845368 33.82729531736851 5 13.363203591802778 39.13450699373883 30.314173044190635 17.188116370267757 6 28.11583901789994 35.14581338387744 20.467993668824224 16.270353929398397 7 26.355987140210534 34.50894448594297 21.127304676316992 18.0077636975295 8 29.08354804706481 28.771640018032663 21.465055134537486 20.67975680036504 9 31.583616601896065 14.344851641860787 20.295959433089937 33.77557232315321 10-11 24.256827238534616 26.755546634286848 26.11898486199638 22.868641265182152 12-13 27.293575918717007 24.849130011769493 28.500437105604053 19.356856963909447 14-15 25.662113528994308 23.161266191280045 29.454040292690735 21.72257998703491 16-17 20.458341148584097 27.100887483423442 29.16632937689691 23.27444199109555 18-19 20.99843737762877 25.195667830754154 34.14289296398729 19.663001827629785 20-21 23.434333263242483 24.218188104321072 32.993683377356746 19.353795255079696 22-23 21.723744567709215 26.364604608205966 31.153477433918553 20.758173390166267 24-25 20.71509905228299 27.276866506499957 31.90668473889437 20.10134970232268 26-27 21.57959101428803 26.616265117530553 32.089623700633055 19.71452016754837 28-29 21.10368890804768 27.28502027577689 32.054209869921316 19.55708094625411 30-31 22.083265646024312 27.286872179413617 31.323606177996655 19.306255996565415 32-33 19.508829313826467 27.91189662150823 31.9772858648713 20.601988199794004 34-35 18.9849058714745 29.13982882132856 31.503281308442993 20.371983998753947 36-37 20.50507909033772 29.180797188484103 30.367322270228886 19.94680145094929 38-39 20.318305052359992 28.507734466597835 30.339508789772363 20.83445169126981 40-41 19.58453737886783 29.301755237323846 30.29254247907747 20.821164904730857 42-43 20.204203843487647 28.969067696958778 30.09910665630919 20.72762180324439 44-45 20.464507562788228 28.53753831273398 29.996279126449316 21.001674998028477 46-47 19.512334367861584 28.08098492780266 30.79567007356246 21.611010630773293 48-49 19.46893610654721 28.484195090128715 30.22601073370843 21.82085806961564 50-51 18.422628520454825 28.070451839217505 30.299574014335363 23.20734562599231 52-53 18.831337350931143 27.020664713350932 30.058483958413802 24.08951397730412 54-55 19.27082588373589 26.719011343339282 30.117656145579563 23.892506627345263 56-57 19.784233095591087 25.64460789032867 30.131747529286855 24.43941148479339 58-59 18.97945410604314 25.88315921477986 30.746709930599764 24.39067674857724 60-61 19.73721053380307 25.35208927703631 30.284964376564854 24.625735812595767 62-63 18.957159769042548 25.406023905504604 30.939111817205628 24.697704508247224 64-65 18.564338024127228 25.43750349355799 31.066093055620964 24.93206542669382 66-67 18.838512307112456 24.905689030479603 31.087037021753066 25.168761640654875 68-69 19.165617126731053 24.628110723574565 31.13211108011552 25.07416106957886 70-71 18.674178264054202 24.778884449875342 30.990478374628484 25.556458911441972 72-73 19.173761595426846 24.444445178111653 30.872888634159633 25.50890459230186 74-75 19.149234488227194 23.923608952055293 30.954959634751038 25.97219692496647 76-77 18.56535561833054 23.9524580368059 30.40018135538943 27.08200498947412 78-79 19.09675021070979 23.841035625828713 29.987213803062197 27.075000360399304 80-81 18.609247192209626 24.11614177114778 29.02111527186267 28.253495764779924 82-83 18.524511123207393 23.723908112409276 28.234316862482732 29.5172639019006 84-85 19.32389647846858 23.206124759029297 27.885660318418072 29.58431844408405 86-87 19.161700268914956 22.651368795198408 27.80784944288632 30.37908149300032 88-89 19.215543331033487 22.619112715929564 27.341768642307525 30.823575310729424 90-91 19.89810476217954 22.537766696940654 26.888696862325695 30.675431678554116 92-93 19.504273209637308 22.99017195267791 26.249397967465704 31.256156870219076 94-95 19.121258688639312 22.792024385776134 26.95571577276651 31.131001152818044 96-97 19.417207152956248 22.655265724010317 26.724285631236434 31.203241491797 98-99 19.737102613650485 22.873623597080066 26.42620115481449 30.963072634454956 100-101 19.679130483426732 22.553552291979962 26.691247686959997 31.07606953763331 102-103 19.38040691954076 23.4660883929541 26.973134184290743 30.180370503214398 104-105 19.233589892933807 23.630400612496285 27.37213427094405 29.763875223625856 106-107 18.501292669898067 23.957686861274638 27.814351323217927 29.726669145609364 108-109 18.534319645401506 24.583027578785956 27.910733931570213 28.971918844242328 110-111 18.39311863057063 25.10363845169188 28.22620681182741 28.277036105910085 112-113 17.734338006462572 25.583137023938953 28.30706523621278 28.375459733385693 114-115 17.855128318422604 25.38746347058764 28.765623509312004 27.991784701677748 116-117 17.659936116112878 25.825342718678783 29.140055707171598 27.374665458036745 118-119 17.468862251104035 26.59055522163704 29.07691614591861 26.863666381340316 120-121 17.452824279713568 27.23174677687796 29.200497827663103 26.11493111574537 122-123 17.108161064029826 27.977566965254464 28.937760008104245 25.976511962611465 124-125 16.500962241416474 28.99905503450523 29.236563309123277 25.263419414955024 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 97.0 1 54.0 2 37.5 3 108.5 4 168.0 5 370.5 6 909.0 7 1721.0 8 3143.5 9 4317.5 10 4782.5 11 5411.0 12 6223.5 13 7213.5 14 8244.0 15 9429.5 16 10893.5 17 12372.5 18 14055.5 19 16026.0 20 18132.0 21 20639.5 22 23707.5 23 27477.5 24 31611.5 25 36530.0 26 42602.5 27 48981.0 28 55755.0 29 63787.0 30 72861.0 31 81817.0 32 90223.0 33 96420.0 34 99631.0 35 99738.5 36 98623.0 37 97050.0 38 93139.5 39 87632.0 40 82486.0 41 79228.0 42 77714.0 43 82099.0 44 90051.5 45 98544.5 46 113880.5 47 125337.5 48 150950.0 49 183246.0 50 219515.5 51 241198.0 52 234231.0 53 231653.0 54 219754.0 55 194763.0 56 163327.5 57 129467.5 58 102421.0 59 82441.5 60 62893.5 61 48724.0 62 38347.0 63 27254.0 64 18536.5 65 13853.5 66 10696.0 67 8178.0 68 6446.5 69 5217.0 70 4451.0 71 3816.5 72 3247.0 73 3180.5 74 2632.5 75 1987.5 76 1506.5 77 1033.0 78 733.5 79 475.0 80 322.5 81 256.5 82 180.0 83 93.0 84 50.5 85 40.5 86 24.0 87 10.0 88 6.0 89 2.5 90 1.5 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12252119441162952 2 0.001908566502025384 3 0.001425940490018965 4 0.001798878772023925 5 0.010113208706134505 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 7.020014720093366E-4 20-21 5.594074230074401E-4 22-23 1.0968773000145885E-4 24-25 4.2778214700568955E-4 26-27 0.0015246594470202778 28-29 0.0 30-31 0.001239471349016485 32-33 0.0 34-35 0.0 36-37 0.0 38-39 1.0968773000145885E-5 40-41 0.0033125694460440576 42-43 0.2827310928517603 44-45 0.2724533525506236 46-47 0.28531972327979477 48-49 0.2932501461589002 50-51 0.3018277266450143 52-53 0.29557552603493115 54-55 0.30637976744007483 56-57 0.30509642099905776 58-59 0.3046247637600515 60-61 0.3037143556010394 62-63 0.30350594891403665 64-65 0.31407984608617723 66-67 0.3242917737493131 68-69 0.3233045841792999 70-71 0.32561899528233074 72-73 0.3292606279183792 74-75 0.3332861676094327 76-77 0.3253338071843269 78-79 0.32519121313532506 80-81 0.5519376885943408 82-83 0.5151922990438521 84-85 0.48952537022351067 86-87 0.45851664895209826 88-89 0.30463573253305165 90-91 0.012646995269168205 92-93 0.08844121670017627 94-95 0.0 96-97 0.0 98-99 1.0968773000145885E-5 100-101 0.0 102-103 0.0 104-105 0.0 106-107 0.0 108-109 0.0 110-111 0.0 112-113 0.23066232742006781 114-115 0.20447986626871958 116-117 0.19504672148859412 118-119 0.17659724530234874 120-121 0.1804582533984001 122-123 0.1688203852452453 124-125 6.032825150080237E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 4558395.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.38747784950771 #Duplication Level Percentage of deduplicated Percentage of total 1 70.31010812920852 24.177872858896503 2 15.418147377510271 10.603824028491598 3 6.240103263051663 6.437442382104896 4 2.8436660551012167 3.911460139247603 5 1.4014046277079604 2.4095385296752543 6 0.8127888529423621 1.6769855206129354 7 0.5276338413225022 1.270079792178975 8 0.36087238970118174 0.9927593045878643 9 0.2563916126573918 0.793499481095006 >10 1.4781532927202419 9.585900689245301 >50 0.16761945642067275 4.041210838617438 >100 0.14630566484383384 10.306182985386812 >500 0.019813379755300044 4.794373429018891 >1k 0.01513254892628818 10.688403723331568 >5k 0.0012182984305062518 2.981809392820901 >10k+ 6.412097002664483E-4 5.328656904688598 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75479 1.6558240345560225 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30504 0.6691829031929001 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28448 0.6240793086163002 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 23632 0.5184280870788951 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 22625 0.4963369782566012 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16506 0.3621011342808159 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 11584 0.25412453286737985 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 11537 0.2530934682053661 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 11025 0.24186144465321674 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 10327 0.22654903754501307 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 9519 0.20882350037677733 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 9514 0.2087138126467759 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9327 0.20461149154472133 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 9282 0.2036243019747082 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 8685 0.19052758701253403 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 7619 0.167142162976223 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 7472 0.1639173437141801 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 7239 0.1588058954961121 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 6851 0.1502941276479989 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6664 0.14619180654594435 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 6636 0.1455775552579362 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 6364 0.13961054274585682 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 6294 0.1380749145258364 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 5888 0.12916827084971794 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 5770 0.12657964042168351 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5710 0.125263387661666 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 5704 0.12513176238566426 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5646 0.12385938471764733 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 5048 0.11074073220947284 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 4849 0.1063751605554148 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT 4767 0.10457628178339087 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA 4630 0.10157083798135089 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.775018400116708E-5 2 0.0 0.0 0.0 0.0 8.775018400116708E-5 3 0.0 0.0 0.0 0.0 8.775018400116708E-5 4 0.0 0.0 0.0 6.58126380008753E-5 8.775018400116708E-5 5 0.0 0.0 0.0 6.58126380008753E-5 8.775018400116708E-5 6 2.4131300600320946E-4 0.0 0.0 6.58126380008753E-5 8.775018400116708E-5 7 2.4131300600320946E-4 0.0 0.0 6.58126380008753E-5 8.775018400116708E-5 8 2.4131300600320946E-4 0.0 0.0 6.58126380008753E-5 8.775018400116708E-5 9 2.4131300600320946E-4 0.0 0.0 8.775018400116708E-5 8.775018400116708E-5 10-11 2.7421932500364707E-4 0.0 0.0 2.4131300600320946E-4 8.775018400116708E-5 12-13 2.85188098003793E-4 0.0 0.0 5.045635580067107E-4 1.0968773000145884E-4 14-15 2.85188098003793E-4 0.0 0.0 7.458765640099202E-4 1.0968773000145884E-4 16-17 3.510007360046683E-4 0.0 0.0 0.0018975977290252382 1.0968773000145884E-4 18-19 3.510007360046683E-4 0.0 0.0 0.003345475765044495 1.316252760017506E-4 20-21 3.510007360046683E-4 0.0 0.0 0.006449638524085781 1.316252760017506E-4 22-23 3.6196950900481417E-4 0.0 0.0 0.019392790664257926 1.316252760017506E-4 24-25 3.729382820049601E-4 0.0 0.0 0.04975435432866174 1.316252760017506E-4 26-27 3.729382820049601E-4 0.0 0.0 0.09441919798525578 1.425940490018965E-4 28-29 3.9487582800525186E-4 0.0 0.0 0.20726593461075665 1.5356282200204238E-4 30-31 3.9487582800525186E-4 0.0 0.0 0.4925198452525505 1.5356282200204238E-4 32-33 3.9487582800525186E-4 0.0 0.0 0.9737308855419506 1.5356282200204238E-4 34-35 3.9487582800525186E-4 0.0 0.0 1.3493016730669458 1.7550036800233416E-4 36-37 3.9487582800525186E-4 2.193754600029177E-5 0.0 1.8056245674190148 1.7550036800233416E-4 38-39 3.9487582800525186E-4 4.387509200058354E-5 0.0 2.402808444638957 1.7550036800233416E-4 40-41 3.9487582800525186E-4 4.387509200058354E-5 0.0 3.259173459079347 1.7550036800233416E-4 42-43 3.9487582800525186E-4 4.387509200058354E-5 0.0 4.013748260078383 1.7550036800233416E-4 44-45 3.9487582800525186E-4 4.387509200058354E-5 0.0 4.6801670324752465 1.7550036800233416E-4 46-47 4.3875092000583536E-4 4.387509200058354E-5 0.0 5.338414068987001 1.8646914100248004E-4 48-49 4.3875092000583536E-4 4.387509200058354E-5 0.0 5.9453272478580725 1.9743791400262593E-4 50-51 4.3875092000583536E-4 4.387509200058354E-5 0.0 6.5710299348783945 1.9743791400262593E-4 52-53 4.3875092000583536E-4 4.387509200058354E-5 0.0 7.272088092409719 2.193754600029177E-4 54-55 4.3875092000583536E-4 4.387509200058354E-5 0.0 7.913300624452248 2.4131300600320946E-4 56-57 4.3875092000583536E-4 4.387509200058354E-5 0.0 8.516078575902263 2.4131300600320946E-4 58-59 4.3875092000583536E-4 4.387509200058354E-5 0.0 9.171287262292978 2.4131300600320946E-4 60-61 4.71657239006273E-4 4.387509200058354E-5 0.0 9.788390431281186 2.4131300600320946E-4 62-63 5.045635580067107E-4 5.484386500072942E-5 0.0 10.411153487137469 2.632505520035012E-4 64-65 5.045635580067107E-4 6.58126380008753E-5 0.0 11.041550809001851 2.632505520035012E-4 66-67 5.045635580067107E-4 6.58126380008753E-5 0.0 11.637440371007777 2.632505520035012E-4 68-69 5.045635580067107E-4 6.58126380008753E-5 0.0 12.283128162434366 2.632505520035012E-4 70-71 5.045635580067107E-4 6.58126380008753E-5 0.0 13.008010494922006 2.632505520035012E-4 72-73 5.045635580067107E-4 6.58126380008753E-5 0.0 13.673628546889859 3.0712564400408476E-4 74-75 5.045635580067107E-4 9.871895700131295E-5 0.0 14.292881595386095 3.2906319000437653E-4 76-77 5.045635580067107E-4 1.316252760017506E-4 0.0 14.930759620436579 3.2906319000437653E-4 78-79 5.265011040070024E-4 1.316252760017506E-4 0.0 15.547577601326783 3.2906319000437653E-4 80-81 5.265011040070024E-4 1.316252760017506E-4 0.0 16.176417357425144 3.2906319000437653E-4 82-83 5.70376196007586E-4 1.316252760017506E-4 0.0 16.888981757833623 3.510007360046683E-4 84-85 6.142512880081695E-4 1.316252760017506E-4 0.0 17.514015349700937 3.510007360046683E-4 86-87 6.142512880081695E-4 1.425940490018965E-4 0.0 18.117506271395964 3.510007360046683E-4 88-89 6.142512880081695E-4 1.5356282200204238E-4 0.0 18.716203839289925 3.510007360046683E-4 90-91 6.361888340084613E-4 1.5356282200204238E-4 0.0 19.32024319963496 3.510007360046683E-4 92-93 6.800639260090449E-4 1.5356282200204238E-4 0.0 19.90137976195569 3.510007360046683E-4 94-95 6.800639260090449E-4 1.5356282200204238E-4 0.0 20.49841007635363 3.510007360046683E-4 96-97 6.910326990091908E-4 1.5356282200204238E-4 0.0 21.08041317174137 3.510007360046683E-4 98-99 7.020014720093366E-4 1.5356282200204238E-4 0.0 21.674931637122278 3.6196950900481417E-4 100-101 7.239390180096283E-4 1.5356282200204238E-4 0.0 22.32024649026686 3.729382820049601E-4 102-103 8.336267480110873E-4 1.5356282200204238E-4 0.0 22.907064877001666 3.729382820049601E-4 104-105 8.336267480110873E-4 1.5356282200204238E-4 0.0 23.467119457616114 3.729382820049601E-4 106-107 8.336267480110873E-4 1.5356282200204238E-4 0.0 24.025309346820535 3.729382820049601E-4 108-109 8.336267480110873E-4 1.5356282200204238E-4 0.0 24.602650713683218 3.83907055005106E-4 110-111 8.336267480110873E-4 1.5356282200204238E-4 0.0 25.163736358959678 3.9487582800525186E-4 112-113 8.336267480110873E-4 1.5356282200204238E-4 0.0 25.751980686184503 3.9487582800525186E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 1260 0.0 63.246685 1 TACAACG 770 0.0 57.852985 2 TAACGCA 1265 0.0 47.890633 4 ATAACGC 1405 0.0 47.76971 3 GACCGTT 900 0.0 47.508247 7 ATAGTAC 1700 0.0 47.165646 4 AGATTAC 2305 0.0 47.15583 2 GTATAAC 1330 0.0 44.26771 1 TCACGCA 830 0.0 43.650852 4 TAGTACT 1865 0.0 43.62783 5 GCATAGT 1945 0.0 42.44734 2 GATTACT 2690 0.0 40.40632 3 AAACGCA 1345 0.0 38.416595 5 ACAACGC 1220 0.0 37.48706 3 GTACAAC 1285 0.0 36.56179 1 TATCACG 1010 0.0 35.872673 2 AGTGTAC 2020 0.0 35.578243 3 TTAGGAC 1320 0.0 35.54706 3 TACTGGT 3685 0.0 35.46014 2 TTCAACG 2690 0.0 34.66477 4 >>END_MODULE