FastQCFastQC Report
Fri 10 Feb 2017
SRR3556981.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556981.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65590
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT14122.1527671901204455No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT8401.2806830309498398RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT4790.7302942521725874RNA PCR Primer, Index 26 (100% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2890.4406159475529806No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC1100.16770849214819333RNA PCR Primer, Index 26 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT1030.15703613355694465RNA PCR Primer, Index 26 (100% over 21bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000.15246226558926665No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT960.14636377496569597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.13416679371855467No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG750.11434669919195RNA PCR Primer, Index 26 (96% over 27bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT710.10824820856837933No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCC150.00420961659.56613586-87
GAGGGTG300.00423853759.429836
CATCGGA350.007799993350.939853
GCACTGA350.007799993350.939856
CCGCACT350.007799993350.939854
GGAGGAT602.1858194E-549.5248576
TACTGGT558.518897E-443.221692
TACCGTC558.518897E-443.221697
GCCTTGG705.38459E-542.5147321
CCTTGGA705.4334014E-542.4498752
ACCCTGG300.001282591339.80204480-81
ACCACGG300.001297163939.71075456-57
TTGGAGG758.160938E-539.6198844
CATGGGG1707.2759576E-1238.4545944
CCAGCAC1252.9327566E-838.0350883
GCGAACC401.1999925E-437.14364228-29
CTTGGAG801.19344404E-437.1436423
ACATGGG3350.035.4804953
TACATGG3550.035.1556742
GAGGATG851.7047692E-434.958727
Produced by FastQC (version 0.11.5)