FastQCFastQC Report
Fri 10 Feb 2017
SRR3556975.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556975.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139279
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT130379.360348652704284No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT30992.225030334795626RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCT16711.1997501418017074RNA PCR Primer, Index 26 (100% over 24bp)
TCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG4780.3431960309881605No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC4630.33242628106175376RNA PCR Primer, Index 26 (95% over 22bp)
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCT3440.2469862649789272RNA PCR Primer, Index 26 (100% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC3160.22688273178296797No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTG1910.13713481572957875RNA PCR Primer, Index 26 (96% over 27bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCT1580.11344136589148399RNA PCR Primer, Index 26 (100% over 27bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG1480.10626153260721286RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.10482556595035863No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGGG350.00781611550.9435233
TTGAGTG505.3454953E-447.5472875
GGGTTGT558.5188594E-443.255871
ACACCGT558.5490634E-443.2248086
TGAGTGT650.001943605136.5748376
CTTGACA452.3837404E-433.06644456-57
AAGGACG750.00392000131.698194
GCTTTGG750.00392000131.698198
CTTATAC57550.029.764251
TATACAC57750.029.7427853
TTATACA57950.029.6401352
TACACAT59850.028.7984835
CATCTCC59700.028.7712869
CACATCT60450.028.4143227
ACATCTC60450.028.4143228
ACACATC60650.028.3206236
ATACACA60900.028.2043654
CTTGAAA32550.026.51410556-57
GCTTGAA32450.026.50410556-57
TTTGTCC450.00956834526.41516132-33
Produced by FastQC (version 0.11.5)