FastQCFastQC Report
Fri 10 Feb 2017
SRR3556974.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556974.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129576
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT62774.844261282953633No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT14631.129067111193431RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT6940.5355930110514293RNA PCR Primer, Index 17 (95% over 23bp)
CTCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC2950.2276656170895845No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC2890.2230351299623387No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG2180.16824103228993023No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCT2010.15512131876273386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.12270790887201333No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTATG300.004233077659.4822271
TATACGA453.1832422E-452.8118674
TTCTATA653.533093E-545.7025762
CTCTTGA558.5128285E-443.25981
AACGCAG3700.038.538396
CGCAGAG3800.035.9607128
GCAGAGT3800.035.9607129
GTATCAA3600.034.6979641
TACACCG700.002799837433.950495
TCTATAC700.002799837433.950493
TATCAAC3700.033.7210922
GTACTTT1900.032.83369414-15
TCAACGC4000.032.6773454
ACGCAGA4250.032.153117
CAACGCA4100.031.8803375
CTATTAA750.003903489831.7238541
CATGGGC750.003925707731.6871224
AGTACTT1900.031.27018512-13
ATCAACG4050.030.8069233
TACCAGG800.005383082729.7066782
Produced by FastQC (version 0.11.5)