FastQCFastQC Report
Fri 10 Feb 2017
SRR3556973.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556973.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98242
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT64536.568473768856498No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT20662.102970216404389TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT12891.3120661224323609RNA PCR Primer, Index 11 (95% over 23bp)
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC3200.3257262677877079No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC2370.24124101708027115No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2240.2280083874513955No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT2080.21172207406201013No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG2050.20866839030150036TruSeq Adapter, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT1600.16286313389385396TruSeq Adapter, Index 11 (96% over 26bp)
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG1570.1598094501333442No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG1090.110950509965188TruSeq Adapter, Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT1000.10178945868365873No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGACT250.002053218271.382586
CGTATGT150.004218360459.54612486-87
GAGGTAA150.0042354259.48548534-35
CTACAAG350.00775135651.039541
ACCTCTT350.007782662350.9875647
GCATATA505.288395E-447.6369061
TTCAAAG558.479166E-443.284212
ATGGGAC558.500475E-443.2621735
GTTTAAG350.002786717133.9917116-17
ATAACAC700.002779966233.991714
CAACGCA4150.032.967865
GTATCAA4050.032.3460461
AACGCAG4300.031.8178226
CATGGGA1701.2385499E-831.492324
CGCAGAG4400.031.0946858
TCAACGC4450.030.745314
TATCAAC4650.030.7178272
ATCAACG4550.030.069593
CAGAAAT603.6867343E-529.74274332-33
GACAGGC603.6867343E-529.74274326-27
Produced by FastQC (version 0.11.5)