FastQCFastQC Report
Fri 10 Feb 2017
SRR3556958.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556958.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13533744
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1731701.2795424532930428No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT720260.5321956732741508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT708380.5234176145196776No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA282520.2087522861375241No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265070.1958585887245983No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT169310.12510211512793504No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA166720.12318838009644634No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC145080.10719871751674924No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA296650.086.15481
GTATCAA876150.078.634971
TATCAAC1231500.055.9281582
ATCAACG1297850.052.8837743
TCAACGC1343050.051.165934
CAACGCA1365100.050.3307575
AACGCAG1398000.049.307826
ACGCAGA1643400.041.8400847
CGCAGAG1658200.041.488158
GCAGAGT1753300.039.237819
ACGGTAC32700.031.9837883
AGAGTAC1716450.031.96860510-11
GTACATG1220500.030.9743161
TACATGG1247150.030.3678112
ACATGGG1241400.030.2148343
ATGGGAC191700.029.4797215
GTACTGG116550.029.3573061
GGACCGT37200.029.232986
AGTACTT1032000.028.88303412-13
CATGGGA370350.028.6893124
Produced by FastQC (version 0.11.5)