FastQCFastQC Report
Fri 10 Feb 2017
SRR3556944.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556944.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914443
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT324361.1129399339770927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160370.5502595178564137No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133930.4595389239041559No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA74950.25716749306814374No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA74800.25665281496327086No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67680.23222276091863867No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT60390.2072094050218172No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50930.17475037254116824No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT45350.1556043470398975No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA35670.12239045333876833No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG33340.11439578677640977No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC31470.107979466402328No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31290.10736185267648055No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31060.10657267958234214No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT29470.10111709167068973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG5350.045.5563432
CGCATAG7150.044.1045881
AGATTAC12950.036.7230722
CATGGGT41700.034.4900474
GACCGTT5100.033.794217
CATGGGG84350.033.1856464
GGACCGT8000.032.6871576
AACGCAG432950.031.805546
TATCACG6200.031.6403332
ATAGTAC11100.031.5895544
ACAACGC7950.031.3976143
ATAACGC7200.030.5410333
TAACGCA8850.029.5477145
ATGGGGT35200.029.5467595
GATTACT16100.029.5310273
ACATGGG405600.029.4958083
GTATCAA449200.029.206721
AGGACCG8600.029.0245365
TACATGG434150.028.8224622
CGCAGAG486050.028.4408938
Produced by FastQC (version 0.11.5)