FastQCFastQC Report
Fri 10 Feb 2017
SRR3556910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3775416
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540611.4319216743267498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289730.7674121209424339No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215220.5700563858393354No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA136560.3617084845749448No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127100.3366516431566747No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT116910.3096612399799122No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT87320.2312857708925321No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA81410.2156318667929574No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG78410.2076857225799753No Hit
TCATTATGGTACTCCTCCGACGTTCGGTGGAGGCACCAAGCTGGAAATCA74640.19770006801899448No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG67870.17976826924503156No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC65560.17364973820103533No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT65450.17335837957989264No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT59370.1572541939749156No Hit
TTATGGTACTCCTCCGACGTTCGGTGGAGGCACCAAGCTGGAAATCAAAC59120.15659201529050043No Hit
GACATGAGTGTGCCCACTCAGGTCCTGGGGTTGCTGCTGCTGTGGCTTAC58830.15582388801657884No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56570.14983779270946568No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG53830.14258031432827534No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA53420.14149434128583446No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG51790.13717693626344754No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT51450.13627637325264289No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT47810.12663505160755795No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG47100.12475446414381886No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA46910.12425120834366331No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45720.1210992378058471No Hit
CCCTATCTGCATCTGTGGGAGAAACTGTCACCATCACATGTCGAGCAAGT45170.11964244470013372No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44890.11890080457358872No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA44800.11866242024719925No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT44510.11789429297327765No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT43480.11516611679348714No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC43250.11455691240382516No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC43200.11442447666694212No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA42970.11381527227728018No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT42170.11169630048715161No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42090.11148440330813875No Hit
ATCTGCATCTGTGGGAGAAACTGTCACCATCACATGTCGAGCAAGTGAGA42030.11132548042387912No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA41670.11037194311832126No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG40920.10838540706507574No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40170.10639887101183021No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA40100.10621346098019398No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39470.10454477069546772No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT39070.10348528480040346No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC39040.10340582335827363No Hit
GGTATCAGCAGAAACAGGGAAAATCTCCTCAGCTCCTGGTCTATAATGCA38960.10319392617926078No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA38580.10218741457894971No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA38480.10192254310518364No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA37900.10038628855734044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9850.083.952721
TACAACG4850.078.440022
ATAGTAC12000.068.846394
GTACAAC5800.066.671711
TAGTACT13000.064.922975
AGATTAC13850.064.80772
GCATAGT13400.061.6608352
CATAGTA13700.060.303413
AGTGTAC13050.058.7525673
TCACGCA5500.054.0324864
GATTACT17550.051.138443
ACAACGC7550.050.3826073
TAACGCA10100.048.8432274
GAGTTCT15450.047.7473681
AACGTAG1700.045.4936331
GTATCAC7750.045.2904361
GACCGTT3200.044.5773937
ATAACGC11200.044.57683
AAACGCA11100.044.4435275
AGTTCTT17150.042.9790652
Produced by FastQC (version 0.11.5)