##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3556882.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2284966 Sequences flagged as poor quality 0 Sequence length 125 %GC 41 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79739304654861 33.0 33.0 34.0 32.0 34.0 2 32.86925976141439 34.0 33.0 34.0 32.0 34.0 3 32.88058071761243 34.0 33.0 34.0 32.0 34.0 4 32.86076335490331 34.0 33.0 34.0 32.0 34.0 5 32.92603434799467 34.0 33.0 34.0 32.0 34.0 6 36.396388392650046 38.0 37.0 38.0 34.0 38.0 7 36.63395166492631 38.0 38.0 38.0 34.0 38.0 8 36.75376044982726 38.0 38.0 38.0 35.0 38.0 9 36.8107551709741 38.0 38.0 38.0 35.0 38.0 10-11 36.81483356863953 38.0 38.0 38.0 35.0 38.0 12-13 36.828155211062224 38.0 38.0 38.0 35.0 38.0 14-15 36.84994984608086 38.0 38.0 38.0 35.0 38.0 16-17 36.867579430065916 38.0 38.0 38.0 35.0 38.0 18-19 36.80961576671163 38.0 38.0 38.0 35.0 38.0 20-21 36.81257379759699 38.0 38.0 38.0 35.0 38.0 22-23 36.679542058831515 38.0 38.0 38.0 34.5 38.0 24-25 36.603480314367914 38.0 38.0 38.0 34.0 38.0 26-27 36.45506804039972 38.0 38.0 38.0 34.0 38.0 28-29 36.42683107757402 38.0 38.0 38.0 34.0 38.0 30-31 36.28981831677145 38.0 38.0 38.0 33.0 38.0 32-33 36.07746811112288 38.0 38.0 38.0 32.5 38.0 34-35 35.570129271070115 38.0 38.0 38.0 29.0 38.0 36-37 35.384952773914364 38.0 37.0 38.0 27.5 38.0 38-39 35.15872730710217 38.0 37.0 38.0 25.5 38.0 40-41 34.81830648683613 38.0 37.0 38.0 24.0 38.0 42-43 34.368714239074016 38.0 36.0 38.0 15.5 38.0 44-45 33.9085881365412 38.0 35.0 38.0 15.0 38.0 46-47 33.202646122524364 38.0 33.5 38.0 15.0 38.0 48-49 32.540798637704015 38.0 30.0 38.0 15.0 38.0 50-51 32.028163657577394 38.0 26.5 38.0 15.0 38.0 52-53 31.623391989202467 38.0 25.0 38.0 15.0 38.0 54-55 31.2906043240906 38.0 24.0 38.0 15.0 38.0 56-57 31.0084053767102 38.0 24.0 38.0 15.0 38.0 58-59 30.78108361349797 38.0 20.0 38.0 15.0 38.0 60-61 30.618568941507228 38.0 16.0 38.0 15.0 38.0 62-63 30.521820893615047 38.0 15.5 38.0 15.0 38.0 64-65 30.325096303402326 38.0 15.0 38.0 15.0 38.0 66-67 30.12151318662947 38.0 15.0 38.0 14.5 38.0 68-69 29.94975111227038 38.0 15.0 38.0 14.0 38.0 70-71 29.62652485857558 37.5 15.0 38.0 14.0 38.0 72-73 29.475230266008335 37.0 14.5 38.0 14.0 38.0 74-75 29.25755000293221 37.0 14.0 38.0 14.0 38.0 76-77 29.132508317410412 37.0 14.0 38.0 14.0 38.0 78-79 28.98549146901967 37.0 14.0 38.0 14.0 38.0 80-81 28.87582988105731 37.0 14.0 38.0 13.0 38.0 82-83 28.762406967981143 37.0 14.0 38.0 13.0 38.0 84-85 28.66828171622685 37.0 14.0 38.0 13.0 38.0 86-87 28.574486228679113 36.5 14.0 38.0 13.0 38.0 88-89 28.51587375917191 36.0 14.0 38.0 13.0 38.0 90-91 28.453468235413567 36.0 14.0 38.0 13.0 38.0 92-93 28.366199103181405 36.0 14.0 38.0 13.0 38.0 94-95 28.26247020743416 35.5 14.0 38.0 13.0 38.0 96-97 28.205250975288035 35.0 14.0 38.0 13.0 38.0 98-99 28.100507403611257 35.0 14.0 38.0 13.0 38.0 100-101 27.98759872138141 35.0 14.0 38.0 13.0 38.0 102-103 27.87359549332463 34.5 14.0 38.0 13.0 38.0 104-105 27.77417278856666 34.0 14.0 38.0 13.0 38.0 106-107 27.661450761192945 34.0 14.0 38.0 13.0 38.0 108-109 27.435763376785474 34.0 14.0 38.0 13.0 38.0 110-111 27.318557256431824 34.0 14.0 38.0 13.0 38.0 112-113 27.15674937832773 33.0 14.0 38.0 13.0 38.0 114-115 26.97227704919898 32.0 14.0 38.0 13.0 38.0 116-117 26.824031736139617 31.0 14.0 38.0 13.0 38.0 118-119 26.617372205975933 30.0 14.0 38.0 13.0 38.0 120-121 26.46836583126401 28.5 14.0 38.0 13.0 38.0 122-123 26.331571892098175 27.0 14.0 38.0 13.0 38.0 124-125 25.363236695863307 24.5 14.0 36.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.22136631063174406 1101 2 -0.19307085882434905 1101 3 0.003480691131102276 1101 4 0.00515974651889195 1101 5 -0.09492883239285277 1101 6 0.09690222728436027 1101 7 0.29096306725413257 1101 8 0.2736322072409294 1101 9 0.28379387366162945 1101 10-11 0.29169359258050065 1101 12-13 0.1842533969286393 1101 14-15 0.122550028540374 1101 16-17 0.13496603190937861 1101 18-19 0.1498420995036227 1101 20-21 0.23020253207553765 1101 22-23 0.17801526350184815 1101 24-25 0.18070336971318568 1101 26-27 0.08565116293480202 1101 28-29 0.21120595749517435 1101 30-31 0.17032280928394528 1101 32-33 0.03429964823027376 1101 34-35 0.02220356428199466 1101 36-37 0.19732427270558617 1101 38-39 0.1943758778524085 1101 40-41 0.20983706711790973 1101 42-43 0.17511296119199926 1101 44-45 0.21489769069508924 1101 46-47 0.21961950536512376 1101 48-49 0.08289758414262138 1101 50-51 0.015815478332875443 1101 52-53 0.00343396553114772 1101 54-55 0.08879747141149252 1101 56-57 0.046682644995811984 1101 58-59 0.11324390276461926 1101 60-61 0.02486964875953035 1101 62-63 0.04948746590858377 1101 64-65 0.12105065519239844 1101 66-67 0.0521340676931743 1101 68-69 0.2773097091765635 1101 70-71 0.29948323590709336 1101 72-73 0.09417492159352392 1101 74-75 0.08890034767266286 1101 76-77 -0.06352700419327562 1101 78-79 0.06565668467974106 1101 80-81 -0.05061478124478569 1101 82-83 0.04029755674510582 1101 84-85 0.10029908902095386 1101 86-87 0.1420272803030862 1101 88-89 0.03560074848741479 1101 90-91 5.258400579783995E-4 1101 92-93 -0.031528377926345996 1101 94-95 -0.043761496776568265 1101 96-97 -0.14667166539983256 1101 98-99 -0.1330057668846436 1101 100-101 -0.04143159033255728 1101 102-103 -0.28626470251555247 1101 104-105 -0.1872271529744154 1101 106-107 -0.188120913729815 1101 108-109 -0.20409293510767768 1101 110-111 -0.1855027316301019 1101 112-113 -0.17055012395355007 1101 114-115 -0.234046230026145 1101 116-117 -0.08963775393229056 1101 118-119 -0.05996111883010968 1101 120-121 0.01761874273701025 1101 122-123 0.09387165938905895 1101 124-125 0.030678261030395504 1102 1 -0.15339955857152177 1102 2 -0.07287628298146842 1102 3 -0.11293671967411001 1102 4 -0.08246640894181212 1102 5 -0.0536499731855713 1102 6 0.26268977730401843 1102 7 0.15701516695055062 1102 8 0.18418153294290818 1102 9 0.2511295429123237 1102 10-11 0.2964537463037189 1102 12-13 0.20790641173586977 1102 14-15 0.16874160931357096 1102 16-17 0.16443273323708496 1102 18-19 0.18950751674334754 1102 20-21 0.14885165492153618 1102 22-23 0.20255813395471733 1102 24-25 0.1682111369716921 1102 26-27 0.20636583959095134 1102 28-29 0.2071218052206163 1102 30-31 0.10555776005859485 1102 32-33 0.027185431943877347 1102 34-35 0.09127050823700955 1102 36-37 0.03540405828111659 1102 38-39 0.0961538486852902 1102 40-41 0.15896772666276604 1102 42-43 0.23189486101713896 1102 44-45 0.2086051929595314 1102 46-47 0.07534679849312909 1102 48-49 0.1490185245233988 1102 50-51 0.12859514592754806 1102 52-53 0.23338906911064328 1102 54-55 0.19161745839138433 1102 56-57 0.3559648799517703 1102 58-59 0.23314630378252943 1102 60-61 0.2826548673055598 1102 62-63 0.15731349264943262 1102 64-65 0.31225537827805994 1102 66-67 0.39936644258939324 1102 68-69 0.4054207785660502 1102 70-71 0.443379522361365 1102 72-73 0.486852887168002 1102 74-75 0.5132237352120192 1102 76-77 0.4050364288498116 1102 78-79 0.3107746988685527 1102 80-81 0.3331503709268233 1102 82-83 0.4111399904540036 1102 84-85 0.5532030253453577 1102 86-87 0.5939297124270624 1102 88-89 0.2973084788630054 1102 90-91 0.3763915329566885 1102 92-93 0.5204030124629178 1102 94-95 0.4748586307731131 1102 96-97 0.4889794771185265 1102 98-99 0.4410675871416281 1102 100-101 0.45907999334386673 1102 102-103 0.3100333794992878 1102 104-105 0.28032940636892434 1102 106-107 0.25408401747023746 1102 108-109 0.3845545361543934 1102 110-111 0.25544999529790147 1102 112-113 0.1371249643099084 1102 114-115 0.2555461990538781 1102 116-117 0.3253601093772893 1102 118-119 0.39510487416806583 1102 120-121 0.38992538336345817 1102 122-123 0.4479499607609121 1102 124-125 0.42311571006644044 1103 1 -0.12169926880613247 1103 2 -0.028122287324109152 1103 3 -0.04888441488173356 1103 4 -0.04238749971485589 1103 5 0.0748748565594326 1103 6 0.21317401795083413 1103 7 0.24229901305122326 1103 8 0.2565054820907804 1103 9 0.24228901042864237 1103 10-11 0.22619414240784863 1103 12-13 0.11364900000678801 1103 14-15 0.06853857604814806 1103 16-17 0.1491248698536296 1103 18-19 0.16172416163097836 1103 20-21 0.2226200422964979 1103 22-23 0.1532757997688705 1103 24-25 0.073489343564205 1103 26-27 0.019031308136433722 1103 28-29 0.09013382922254465 1103 30-31 0.132642759734928 1103 32-33 0.0387590412400769 1103 34-35 0.07678882182657532 1103 36-37 -0.019731459977620602 1103 38-39 -0.06524333397443627 1103 40-41 -0.08437211295137814 1103 42-43 0.006234166304345479 1103 44-45 0.11068484388056277 1103 46-47 0.2458947414441255 1103 48-49 0.30135601287957314 1103 50-51 0.33382880698871276 1103 52-53 0.3779162775431324 1103 54-55 0.21889720761978992 1103 56-57 0.006169997860279608 1103 58-59 0.2185088177304344 1103 60-61 0.3204831848778973 1103 62-63 0.18320091972806907 1103 64-65 0.3942342421914624 1103 66-67 0.35391107424941737 1103 68-69 0.3004493985213479 1103 70-71 0.49994768415600177 1103 72-73 0.42967002995617776 1103 74-75 0.22393561888454627 1103 76-77 0.16277868357783376 1103 78-79 0.49169291832002315 1103 80-81 0.19132990697304564 1103 82-83 0.3587320631049131 1103 84-85 0.3174833791202616 1103 86-87 0.3176774728072118 1103 88-89 0.3155932674417947 1103 90-91 0.3764721757289564 1103 92-93 0.37068092830258337 1103 94-95 0.33658041430596697 1103 96-97 0.5153894364460427 1103 98-99 0.3601702689801094 1103 100-101 0.40477257212417683 1103 102-103 0.27954904010622883 1103 104-105 0.3028397116374748 1103 106-107 0.08635802295833273 1103 108-109 0.12094318823514172 1103 110-111 0.2686762328353005 1103 112-113 0.30806268066020337 1103 114-115 0.09257675663007703 1103 116-117 0.29944772144057197 1103 118-119 0.2895714633737825 1103 120-121 0.3641391711928712 1103 122-123 0.37824306157973453 1103 124-125 0.26129117557272963 1104 1 -0.01875113943386708 1104 2 -0.023343068821311874 1104 3 -0.052138404822066775 1104 4 0.018877688514066904 1104 5 0.0362512454128634 1104 6 0.24259102862236404 1104 7 0.2714195973714837 1104 8 0.12273172837164026 1104 9 0.025493967792684202 1104 10-11 0.2103876644508702 1104 12-13 0.1390129190347693 1104 14-15 0.13047067829767656 1104 16-17 0.2151598604764544 1104 18-19 0.28740893456966177 1104 20-21 0.2043744299768946 1104 22-23 0.21025267471081577 1104 24-25 0.16875501454978803 1104 26-27 0.256157450133891 1104 28-29 0.21738131408026362 1104 30-31 0.20248339752419753 1104 32-33 0.05125983119727806 1104 34-35 0.24139526197425454 1104 36-37 0.10608251283988324 1104 38-39 0.05054347880127352 1104 40-41 0.05922508691512718 1104 42-43 0.10234556684007856 1104 44-45 0.23920766825691686 1104 46-47 0.22123177929199755 1104 48-49 0.2593195149550098 1104 50-51 0.20521499040618352 1104 52-53 0.29327813408110615 1104 54-55 0.29431761424720904 1104 56-57 0.3004519208687384 1104 58-59 0.12942219659065657 1104 60-61 0.2736995194365903 1104 62-63 0.2939536452841658 1104 64-65 0.26352389274698496 1104 66-67 0.1793813228763419 1104 68-69 0.30230935164723505 1104 70-71 0.40278656855715056 1104 72-73 0.2945874371002155 1104 74-75 0.3335958041899474 1104 76-77 0.2025413722825391 1104 78-79 0.41528617428553716 1104 80-81 0.3980368564778942 1104 82-83 0.3482205166919172 1104 84-85 0.22902935752731324 1104 86-87 0.45870805868294084 1104 88-89 0.37715805090479293 1104 90-91 0.348736121574742 1104 92-93 0.39463704851080195 1104 94-95 0.36069869077415007 1104 96-97 0.5279511594925665 1104 98-99 0.4783571935902131 1104 100-101 0.37335083565245597 1104 102-103 0.4098886302628486 1104 104-105 0.2942622702079376 1104 106-107 0.2619760592252014 1104 108-109 0.32123242574942523 1104 110-111 0.29498936807181053 1104 112-113 0.3285247001511884 1104 114-115 0.39165328985780334 1104 116-117 0.4012620245462024 1104 118-119 0.2769890973192908 1104 120-121 0.33054702162015204 1104 122-123 0.36736564501234525 1104 124-125 0.4191765058364112 1105 1 -0.05386884836446626 1105 2 0.005949669220740361 1105 3 0.06320728252718055 1105 4 0.058812351578481525 1105 5 0.011631356945500215 1105 6 0.11348335587702252 1105 7 0.2214428489818019 1105 8 -0.013049093957626212 1105 9 0.06314333994556875 1105 10-11 0.28724777986681715 1105 12-13 0.09707813475089466 1105 14-15 0.02348466448545139 1105 16-17 0.21580761583339125 1105 18-19 0.20113088893500475 1105 20-21 0.25250544211833414 1105 22-23 0.2773452365019011 1105 24-25 0.21581176832462035 1105 26-27 0.2509616404656114 1105 28-29 0.3302659601685818 1105 30-31 0.22783364222483016 1105 32-33 0.11701835307784592 1105 34-35 0.17968212695097208 1105 36-37 -0.04106352854887518 1105 38-39 0.07030797333307248 1105 40-41 0.16256539841340611 1105 42-43 0.21561461094591294 1105 44-45 0.23851727153849112 1105 46-47 0.2993557596315526 1105 48-49 0.11643635818551701 1105 50-51 -0.06374758728467711 1105 52-53 -0.11572537931677118 1105 54-55 -0.0492992600033233 1105 56-57 -0.1275162709998625 1105 58-59 -0.08653949305139008 1105 60-61 -0.056197116830450256 1105 62-63 0.03159431037498095 1105 64-65 0.04107883886779362 1105 66-67 -0.08188444437047693 1105 68-69 0.022245716790308734 1105 70-71 0.04515078800300287 1105 72-73 -0.07751467117093824 1105 74-75 0.023558510430657265 1105 76-77 -0.0020331965525066664 1105 78-79 0.13449826204771753 1105 80-81 0.11223317587581505 1105 82-83 0.07544775009356286 1105 84-85 -0.09355701179050158 1105 86-87 0.1548417834228495 1105 88-89 0.2109110038414208 1105 90-91 0.16139733408051526 1105 92-93 0.023017851423503544 1105 94-95 -0.09535013385292501 1105 96-97 0.08926335844410715 1105 98-99 0.12704154220810437 1105 100-101 -0.048807314273854985 1105 102-103 -0.034119239347916164 1105 104-105 -0.016602552027649153 1105 106-107 0.0264865927142246 1105 108-109 0.0470298773157225 1105 110-111 0.24419251098152017 1105 112-113 0.16186044714649128 1105 114-115 -0.017509882809672916 1105 116-117 0.30017884456980326 1105 118-119 0.36245391765970325 1105 120-121 0.13112186650661428 1105 122-123 0.4384398551057096 1105 124-125 0.3032233802246118 1106 1 0.013100221587599492 1106 2 -0.016176592042441484 1106 3 -0.02184022183026002 1106 4 -0.019793036860853874 1106 5 0.06399110742596292 1106 6 0.09118821595237137 1106 7 0.19861055917128567 1106 8 0.10323622785618625 1106 9 0.07615278399806868 1106 10-11 0.2562651217587728 1106 12-13 0.15853234311769882 1106 14-15 0.11105798334046568 1106 16-17 0.23172008933759258 1106 18-19 0.21583332841620262 1106 20-21 0.2433591632097034 1106 22-23 0.15715992242179055 1106 24-25 0.2461744155213097 1106 26-27 0.21888206021063894 1106 28-29 0.25666195800736347 1106 30-31 0.1966360715522626 1106 32-33 0.16648217648022268 1106 34-35 0.23090102578844096 1106 36-37 0.11437507458784779 1106 38-39 0.17001530293003242 1106 40-41 0.08977080379321478 1106 42-43 0.08893062500965954 1106 44-45 0.1704820563918119 1106 46-47 0.1623433441563762 1106 48-49 0.110083153463016 1106 50-51 0.06171163377959488 1106 52-53 0.04465647585477939 1106 54-55 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-0.15261531340243195 2313 90-91 -0.23717730685956795 2313 92-93 -0.017856430974788395 2313 94-95 -0.07550572648312937 2313 96-97 -0.08544562858897464 2313 98-99 -0.01897322087106801 2313 100-101 -0.10591010316251825 2313 102-103 -0.04704902318659521 2313 104-105 -0.07829471039396907 2313 106-107 0.09567816387336947 2313 108-109 0.10909974806106248 2313 110-111 0.0025739434906881797 2313 112-113 -0.09314601213241858 2313 114-115 -0.01664254327267045 2313 116-117 0.020735845115556373 2313 118-119 0.06611731323699388 2313 120-121 0.19203321204004098 2313 122-123 -0.04154302953226008 2313 124-125 0.2783334815696641 2314 1 0.12426357906870322 2314 2 0.06787288974820171 2314 3 0.03785581708217478 2314 4 -0.017086011449052307 2314 5 0.0543786046540049 2314 6 -0.3180063490319185 2314 7 -0.18997969610116172 2314 8 -0.12317300187143587 2314 9 -0.08480949130314741 2314 10-11 -0.3084660573986753 2314 12-13 -0.23936763477296097 2314 14-15 -0.11936559207932618 2314 16-17 -0.16283827707266596 2314 18-19 -0.12231969108396612 2314 20-21 -0.07370788480571377 2314 22-23 -0.17685327716747423 2314 24-25 -0.32354314060248157 2314 26-27 -0.263624030638006 2314 28-29 -0.1441910178878274 2314 30-31 -0.01693340542176003 2314 32-33 -0.03545709267101671 2314 34-35 -0.21236323120929512 2314 36-37 -0.09855550391878865 2314 38-39 0.05096630960147763 2314 40-41 -0.01484732748847506 2314 42-43 -0.11727153327958462 2314 44-45 0.06411242775403281 2314 46-47 0.17945734756018794 2314 48-49 0.14340223929418272 2314 50-51 0.13631986478883107 2314 52-53 0.16469275971478226 2314 54-55 0.02326261039430122 2314 56-57 0.1243256022597734 2314 58-59 0.2666153610205164 2314 60-61 0.13797818835407227 2314 62-63 0.02851017516649179 2314 64-65 0.013348495327463183 2314 66-67 0.05718330616501177 2314 68-69 -0.06361912678923787 2314 70-71 0.017727892223010855 2314 72-73 0.0638246320739988 2314 74-75 -0.014581051873239659 2314 76-77 0.14750877262814655 2314 78-79 0.1941055048820708 2314 80-81 0.059848226086128165 2314 82-83 0.16398262756878879 2314 84-85 0.11225940267830481 2314 86-87 0.11585856632509106 2314 88-89 0.11388969479755318 2314 90-91 0.041134704326392324 2314 92-93 0.08730449787806194 2314 94-95 -0.07122106928184024 2314 96-97 0.11611040901989256 2314 98-99 0.004509291265566873 2314 100-101 -0.026677961822162644 2314 102-103 0.023981333104529767 2314 104-105 0.004313637259397041 2314 106-107 0.008685191034604856 2314 108-109 -0.03886528274606604 2314 110-111 0.030685283649724227 2314 112-113 -0.040226032256693856 2314 114-115 0.062350785661202224 2314 116-117 0.007073635627094177 2314 118-119 0.11374723894706307 2314 120-121 0.3119176934806269 2314 122-123 0.0026830843266978377 2314 124-125 0.3315960804758973 2315 1 0.12894417727482477 2315 2 0.15064707764140906 2315 3 -0.020543746029709098 2315 4 0.009300810491758682 2315 5 0.1197016434155529 2315 6 -0.181713366668383 2315 7 -0.18348674831545964 2315 8 -0.160968247210171 2315 9 0.011072631555975931 2315 10-11 -0.2640054594153227 2315 12-13 -0.17592377668798775 2315 14-15 -0.12252432997890139 2315 16-17 -0.15593780488029552 2315 18-19 -0.07596272036648344 2315 20-21 0.006522766421355186 2315 22-23 -0.05099700373724403 2315 24-25 -0.1740316452502313 2315 26-27 -0.3247690765569402 2315 28-29 -0.16875604672447508 2315 30-31 -0.0937527650022858 2315 32-33 -0.1983088982648482 2315 34-35 -0.2062016946364622 2315 36-37 -0.15444518805468732 2315 38-39 -0.04248689030857378 2315 40-41 -0.2943245630249933 2315 42-43 -0.3913716904940898 2315 44-45 -0.3132298286607451 2315 46-47 -0.2790851759852586 2315 48-49 -0.2848004190660518 2315 50-51 -0.36859520725396777 2315 52-53 -0.16721492426323437 2315 54-55 -0.17098003588468558 2315 56-57 -0.10435959599209355 2315 58-59 -0.1538259308471872 2315 60-61 -0.08146979001331545 2315 62-63 -0.11270934061154136 2315 64-65 -0.18241972409678553 2315 66-67 -0.18225725943915094 2315 68-69 -0.1691920815679957 2315 70-71 -0.08230373147723569 2315 72-73 -0.1414142317908933 2315 74-75 -0.02546838642793503 2315 76-77 -0.1318807269150959 2315 78-79 0.08796012722906354 2315 80-81 -0.06408746044439084 2315 82-83 -0.06369865137823538 2315 84-85 -0.136901051844184 2315 86-87 -0.03401073368050689 2315 88-89 0.08411240539259168 2315 90-91 -0.06251725998322044 2315 92-93 0.01170206154557718 2315 94-95 -0.031333316467790695 2315 96-97 0.01578304964406385 2315 98-99 0.1963789493179604 2315 100-101 0.07081173948964903 2315 102-103 0.04763074362681152 2315 104-105 -0.04384042811336286 2315 106-107 0.06687053949501376 2315 108-109 0.005607260892301014 2315 110-111 -0.24157871338904613 2315 112-113 -0.07972151558838547 2315 114-115 -0.23718812819787516 2315 116-117 -0.18970302307957 2315 118-119 -0.3185329234686485 2315 120-121 -0.5575255792672529 2315 122-123 -0.6171974558945656 2315 124-125 -0.36741619142464543 2316 1 0.09635771159121731 2316 2 0.021668596401006823 2316 3 -0.013871312108726386 2316 4 0.04967421967023711 2316 5 -0.012067301645188877 2316 6 -0.3703965677738523 2316 7 -0.32635527545564713 2316 8 -0.31333450565053056 2316 9 -0.2086609081497599 2316 10-11 -0.4606565084887393 2316 12-13 -0.4663424119847406 2316 14-15 -0.2957667345789048 2316 16-17 -0.29209142416100065 2316 18-19 -0.4045708485802706 2316 20-21 -0.3071567993421809 2316 22-23 -0.35220295140310753 2316 24-25 -0.18489881190991753 2316 26-27 -0.13105357636264614 2316 28-29 -0.13671278738780757 2316 30-31 -0.20702821219041567 2316 32-33 -0.2979070800187955 2316 34-35 -0.20946906602545567 2316 36-37 -0.12873432466713552 2316 38-39 -0.1100720456259836 2316 40-41 -0.3269699335710854 2316 42-43 -0.46060971460976674 2316 44-45 -0.322023001940444 2316 46-47 -0.20944960566377802 2316 48-49 -0.4215290385316095 2316 50-51 -0.5044910253373445 2316 52-53 -0.40811781996712426 2316 54-55 -0.4047063969197353 2316 56-57 -0.3104355052178107 2316 58-59 -0.15814184165027356 2316 60-61 -0.24453652338953802 2316 62-63 -0.1368399598848491 2316 64-65 -0.39096667998057555 2316 66-67 -0.28502628090368987 2316 68-69 -1.8728384322186997 2316 70-71 -1.5179621227090365 2316 72-73 -0.4269089299424955 2316 74-75 -0.2232905496083717 2316 76-77 -0.24774231938381774 2316 78-79 -0.14783373697099123 2316 80-81 -0.039258033974007844 2316 82-83 -0.22184276208323084 2316 84-85 -0.1328297974992303 2316 86-87 -0.33946801861819864 2316 88-89 -0.3194052911233136 2316 90-91 -0.3444670684397231 2316 92-93 -0.4155063162539463 2316 94-95 -0.21070133383856415 2316 96-97 -0.4303314065293229 2316 98-99 -0.3472153317346347 2316 100-101 -0.08796709780887468 2316 102-103 -0.34949556992286546 2316 104-105 -0.5422914849093132 2316 106-107 -0.41536122361706873 2316 108-109 -0.23388232888503424 2316 110-111 -0.5548691948726265 2316 112-113 -0.598690853019594 2316 114-115 -0.6283257807281935 2316 116-117 -0.6635453207142561 2316 118-119 -0.9323094316836986 2316 120-121 -0.9386587708017124 2316 122-123 -1.1278376293393961 2316 124-125 -0.8049343167876657 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 6.0 14 239.0 15 1616.0 16 2249.0 17 2759.0 18 4430.0 19 10256.0 20 28933.0 21 68687.0 22 114309.0 23 169814.0 24 191562.0 25 133487.0 26 73610.0 27 55496.0 28 40033.0 29 34090.0 30 35594.0 31 39660.0 32 50785.0 33 68615.0 34 101547.0 35 142407.0 36 237469.0 37 677312.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.757844392588254 15.132715877675269 11.330163846383995 18.779275883352483 2 14.227284484852676 25.971499387471862 37.978188833736795 21.82302729393866 3 22.00023982851386 37.253858481920524 24.758617852519468 15.987283837046155 4 12.733843742095068 19.342738579042315 34.15433752624766 33.76908015261496 5 12.236768512091647 40.66016737229351 28.927432618253402 18.17563149736145 6 25.360596175172844 32.35759306703032 23.575886905975842 18.70592385182099 7 24.572138053695326 34.745549824373754 22.564230715030334 18.118081406900586 8 29.729457681208387 24.783432226125026 25.031838548144698 20.45527154452189 9 34.35175840690846 13.319585499302836 21.309901328947564 31.018754764841137 10-11 23.47452872384097 27.33493627476295 26.330172965374537 22.86036203602154 12-13 27.511153338824297 25.672548300499876 29.642913723880355 17.173384636795472 14-15 26.640177578134644 22.238317769279718 33.023314132464115 18.098190520121523 16-17 18.33073044711655 26.42587069771574 33.362239703172506 21.88115915199521 18-19 19.856947543201954 24.037753734628875 39.379469978984375 16.7258287431848 20-21 22.329385377061737 23.27853606499084 37.987362892858116 16.40471566508931 22-23 19.515651436388985 27.275766904190256 34.037333597086345 19.171248062334406 24-25 18.67642609235391 28.3274867485079 34.65457167439748 18.341515484740707 26-27 20.365996343371943 26.452535370371834 35.44759023295863 17.73387805329759 28-29 19.636488134497945 27.309973229916036 36.31138933649201 16.74214929909401 30-31 21.222032883626806 27.751806651366355 35.1346766523623 15.891483812644536 32-33 16.009059981005382 29.111230134505433 36.44530139342736 18.434408491061827 34-35 14.809834759516729 30.97152113055874 35.66407986480384 18.554564245120684 36-37 18.343538251416977 30.679682362481053 33.39404969442181 17.582729691680157 38-39 18.566946580214424 29.262137610425775 33.29498181222543 18.875933997134368 40-41 16.32349730318176 31.29128199220946 33.191986434552035 19.193234270056745 42-43 18.141157184704625 30.301212087900296 32.665342992780374 18.892287734614698 44-45 18.995687062786637 29.2077520333685 32.775958248648415 19.020602655196445 46-47 16.781002557641074 28.79567532913125 34.69154051620077 19.73178159702691 48-49 16.9582802897831 29.37410581383586 33.888183101299276 19.779430795081762 50-51 14.877945280107063 28.914632202371465 33.89220156567771 22.315220951843763 52-53 15.271616622440654 27.703761189166602 32.67637376185048 24.348248426542266 54-55 16.254356911535535 26.91246407382069 32.47047724332218 24.3627017713216 56-57 17.663399701904247 24.633720209586805 33.08941690000565 24.6134631885033 58-59 16.442919431063707 24.841232658795267 34.78425711340111 23.93159079673991 60-61 17.568176426922875 24.416407419957327 33.89562654105129 24.119789612068505 62-63 15.420235861476177 24.897036770610498 35.578466939833376 24.10426042807995 64-65 14.885971579968851 25.310191036759406 35.29785831166527 24.505979071606472 66-67 16.20719138133064 24.64353397164132 35.07979726087055 24.06947738615749 68-69 17.201288349175574 23.909812052951644 34.874580031376354 24.014319566496436 70-71 15.823584369383234 24.42780582320102 34.68803497907455 25.0605748283412 72-73 17.34808507135293 24.049465058291098 33.86357152818814 24.73887834216784 74-75 17.35778666932464 23.21615988135517 34.38369203167221 25.042361417647978 76-77 16.247731329863544 23.852738660592927 33.65367846623439 26.245851543309147 78-79 17.24050207241045 23.515816086598996 32.62548964145066 26.61819219953989 80-81 16.428721228618077 24.19679185217844 31.006498695050826 28.367988224152658 82-83 16.161307269662675 24.446577501477574 29.011689249830354 30.380425979029397 84-85 17.409072592868867 23.845198016865528 28.360066162850178 30.385663227415428 86-87 17.719487639503257 22.992276864272526 28.106274689425597 31.18196080679862 88-89 17.933307962940553 23.446969788151385 27.859098469876454 30.760623779031608 90-91 19.20682988872894 23.844692335939417 26.541230866328107 30.407246909003533 92-93 18.022270053946364 25.568743320165506 25.64550944433406 30.763477181554073 94-95 17.817650389090968 26.038205080340354 26.240940742493102 29.903203788075583 96-97 18.746565634217806 25.93116584504492 25.740515460691377 29.5817530600459 98-99 19.81366386231713 26.394927924730283 25.061173275189365 28.73023493776322 100-101 19.489436346181748 26.34586006788521 25.500478907814454 28.664224678118583 102-103 19.289998680498314 27.569532596615204 25.752756018886164 27.38771270400032 104-105 19.30552911390189 27.413306023664003 26.701482118512587 26.579682743921516 106-107 17.961079746320493 28.04678991172949 27.71278571058119 26.279344631368822 108-109 17.86909854809239 28.600610523321336 27.700624746982943 25.829666181603333 110-111 17.456861087186383 29.02108323004853 28.569518552752278 24.952537130012814 112-113 16.37582238965547 29.645109295273393 28.2696567156406 25.70941159943054 114-115 16.672593281822476 28.730982950824117 28.970709974941904 25.625713792411496 116-117 16.57679952763398 28.900400889581817 29.991477977772714 24.53132160501149 118-119 16.14450648443368 30.299188285822844 29.61533309871362 23.94097213102986 120-121 16.257768796927078 30.596843616122904 30.045847417885067 23.09954016906495 122-123 15.267035925732712 32.07514346134859 29.778645878921772 22.879174733996926 124-125 14.298405987857542 33.61710228746049 30.0630536891824 22.021438035499568 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 47.0 1 25.0 2 38.5 3 140.5 4 255.0 5 419.0 6 783.0 7 1335.5 8 2122.5 9 3050.5 10 3762.5 11 4520.5 12 5413.5 13 6247.5 14 7453.0 15 8830.0 16 10112.5 17 11683.0 18 13553.0 19 15507.0 20 17631.0 21 19978.5 22 22642.0 23 25962.5 24 29368.5 25 33147.0 26 37808.0 27 42933.5 28 48293.5 29 53567.5 30 57803.0 31 60289.5 32 61409.5 33 59494.5 34 54504.0 35 47829.5 36 41149.0 37 35920.5 38 30406.0 39 25331.5 40 21072.0 41 17935.5 42 16699.5 43 18125.0 44 22362.5 45 27902.0 46 39357.5 47 52234.0 48 83464.5 49 118904.5 50 167533.0 51 179919.0 52 125367.5 53 99365.0 54 99025.5 55 87765.5 56 66616.5 57 46138.5 58 32357.0 59 23061.5 60 14789.5 61 10127.5 62 7224.5 63 3856.0 64 1331.5 65 305.5 66 209.5 67 167.0 68 149.5 69 138.0 70 125.0 71 108.0 72 92.5 73 83.0 74 67.5 75 53.0 76 43.0 77 35.0 78 33.5 79 23.0 80 15.5 81 12.5 82 7.5 83 7.0 84 4.0 85 3.5 86 3.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09439965408675666 2 0.007571228630973066 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 1.0941081836666278E-4 18-19 0.0 20-21 0.0012472833293799559 22-23 0.0 24-25 0.1253629156845222 26-27 0.27851617923417676 28-29 0.03879707619281862 30-31 0.03056938265164558 32-33 0.027768465701459015 34-35 0.5174912887106417 36-37 0.07315207315995073 38-39 0.07446500298035069 40-41 0.07932284331583052 42-43 0.07328336614199074 44-45 0.054946112983738055 46-47 0.061992169686551135 48-49 0.06411473956286438 50-51 0.06485873312775771 52-53 0.07085444597425082 54-55 0.07930096115215719 56-57 0.07908213951542385 58-59 0.09385259999492333 60-61 0.09761633214673654 62-63 0.10306499090139636 64-65 0.10595343650627624 66-67 0.11052680871400274 68-69 0.10306499090139636 70-71 0.10759459878177619 72-73 0.05306424690783145 74-75 0.11043928005930942 76-77 0.12219000195188899 78-79 0.05041650510335821 80-81 0.04240763319891849 82-83 0.035711691114878735 84-85 0.02636800722636573 86-87 0.00908109792443301 88-89 0.0065865312656730995 90-91 0.008096400559133047 92-93 0.004551490044053172 94-95 0.009518741197899663 96-97 0.006805352902406425 98-99 0.002757152622839902 100-101 0.0034792640240598766 102-103 5.47054091833314E-4 104-105 0.0026696239681465723 106-107 0.005011015481193155 108-109 0.0017943374212132698 110-111 9.628152016266325E-4 112-113 3.93878946119986E-4 114-115 0.0033042067146732165 116-117 0.01299800522195954 118-119 0.018293488830906018 120-121 0.004573372207726504 122-123 0.0013129298203999535 124-125 3.719967824466535E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2284966.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.768352841274574 #Duplication Level Percentage of deduplicated Percentage of total 1 76.113626652509 15.807546543483472 2 10.33946317846145 4.294672389593073 3 4.1818382276000685 2.6054967550778554 4 2.081027483685607 1.728780522142898 5 1.3088181554858638 1.3590998629098292 6 0.8618731600228552 1.0739811535067376 7 0.635053227715431 0.9232306654329052 8 0.4881712187804483 0.8110809694869915 9 0.3765365234609724 0.7038039039177902 >10 2.796848443846274 11.618276682022406 >50 0.3896822093363584 5.669104104291948 >100 0.3328598664005982 14.145173040859294 >500 0.04899327026329432 7.1134396489747145 >1k 0.040372136869324074 17.791568885873303 >5k 0.0023129870081383583 3.382323932521684 >10k+ 0.0025232585543327546 10.972420939905144 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 52392 2.2929006383464787 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 34727 1.5198037957676394 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 28284 1.237830234673076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26530 1.1610676045070256 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19076 0.8348483084649838 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 16841 0.7370350368451872 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 13985 0.6120441179431116 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 12992 0.5685861408878732 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 12241 0.5357191310505277 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 12207 0.5342311439207411 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11087 0.48521509729247614 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 10896 0.4768561107692631 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 9979 0.43672422259237115 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 8540 0.3737473555405201 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 8027 0.3512962556116809 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7750 0.33917353693665464 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 7734 0.338473307699108 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 6656 0.291295362819403 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 6523 0.28547470728229657 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 6210 0.2717764728227903 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 5646 0.24709339219927123 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5353 0.23427044428669835 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 5034 0.22030962386311218 No Hit CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC 4747 0.2077492619146193 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4656 0.20376670812607278 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 4524 0.19798981691631296 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 4462 0.19527642862081973 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 4414 0.19317574090817982 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 4363 0.1909437602134999 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 4356 0.19063740992207323 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 4328 0.18941200875636663 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4280 0.1873113210437267 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 4077 0.17842716259235367 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 4017 0.17580130295155377 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 3985 0.17440084447646048 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 3916 0.17138110588954059 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 3895 0.17046205501526063 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 3872 0.1694554754862873 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3786 0.16569174333447415 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3766 0.16481645678754084 No Hit GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT 3723 0.16293459071163421 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 3720 0.16280329772959423 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3620 0.15842686499492772 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3591 0.15715769950187444 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 3521 0.15409419658760787 No Hit CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT 3511 0.15365655331414121 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA 3505 0.15339396735006122 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 3493 0.15286879542190127 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 3428 0.150024114144368 No Hit GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG 3390 0.14836106970519475 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 3305 0.1446411018807282 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 3261 0.14271547147747493 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 3236 0.1416213632938083 No Hit GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC 3235 0.14157759896646166 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 3182 0.13925808961708838 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 3149 0.13781386681464844 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 3130 0.1369823445950618 No Hit CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA 3110 0.1361070580481285 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 3098 0.13558188611996852 No Hit CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC 3000 0.13129298203999534 No Hit GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA 2940 0.12866712239919545 No Hit GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT 2938 0.12857959374450212 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 2921 0.1278356001796088 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 2918 0.1277043071975688 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 2888 0.12639137737716885 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 2884 0.1262163200677822 No Hit CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGA 2857 0.12503468322942224 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG 2843 0.12442198264656892 No Hit GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA 2810 0.12297775984412897 No Hit GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC 2749 0.12030813587598241 No Hit AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA 2704 0.11833874114538247 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 2703 0.1182949768180358 No Hit GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT 2687 0.11759474758048917 No Hit CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA 2624 0.11483759495764927 No Hit CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA 2612 0.11431242302948928 No Hit TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA 2564 0.11221173531684936 No Hit CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG 2561 0.11208044233480935 No Hit GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2528 0.1106362195323694 No Hit TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG 2503 0.10954211134870277 No Hit CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA 2492 0.10906070374788947 No Hit GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA 2455 0.10744142363606286 No Hit ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT 2422 0.1059972008336229 No Hit GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2403 0.10516567861403626 No Hit GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG 2392 0.10468427101322296 No Hit TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG 2381 0.10420286341240963 No Hit GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC 2356 0.10310875522874302 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2322 0.10162076809895638 No Hit GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA 2319 0.10148947511691642 No Hit CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG 2308 0.10100806751610308 No Hit CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC 2298 0.10057042424263644 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 2291 0.10026407395120977 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 8.752865469333024E-5 0.0 2 0.0 0.0 0.0 8.752865469333024E-5 0.0 3 0.0 0.0 0.0 1.3129298203999533E-4 0.0 4 0.0 0.0 0.0 1.3129298203999533E-4 0.0 5 0.0 0.0 0.0 2.6258596407999067E-4 0.0 6 0.0 0.0 0.0 3.063502914266558E-4 0.0 7 0.0 0.0 0.0 3.063502914266558E-4 0.0 8 0.0 0.0 0.0 3.063502914266558E-4 0.0 9 0.0 0.0 0.0 3.5011461877332094E-4 0.0 10-11 0.0 0.0 0.0 7.87757892239972E-4 0.0 12-13 0.0 0.0 0.0 0.0014004584750932835 0.0 14-15 0.0 0.0 0.0 0.0018162195848866023 0.0 16-17 0.0 0.0 0.0 0.0036324391697732045 0.0 18-19 0.0 0.0 0.0 0.006433356119959772 0.0 20-21 0.0 0.0 0.0 0.012801065748899547 0.0 22-23 0.0 0.0 0.0 0.03820625777363865 0.0 24-25 0.0 0.0 0.0 0.09724433536428989 0.0 26-27 0.0 0.0 0.0 0.17378814389360717 0.0 28-29 0.0 0.0 0.0 0.3518433097035142 0.0 30-31 0.0 0.0 0.0 0.7694644034090661 0.0 32-33 0.0 0.0 0.0 1.4546605945121285 0.0 34-35 0.0 0.0 0.0 2.0032245556389023 0.0 36-37 0.0 0.0 0.0 2.6297765480974333 0.0 38-39 0.0 0.0 0.0 3.362894677645094 0.0 40-41 0.0 0.0 0.0 4.580155678465237 0.0 42-43 0.0 0.0 0.0 5.54218312219963 0.0 44-45 0.0 0.0 0.0 6.335236498048548 0.0 46-47 0.0 0.0 0.0 7.134329351071306 0.0 48-49 0.0 0.0 0.0 7.812391956816863 0.0 50-51 0.0 0.0 0.0 8.500498475688477 0.0 52-53 0.0 0.0 0.0 9.402021736866107 0.0 54-55 0.0 0.0 0.0 10.149844680402246 0.0 56-57 0.0 0.0 0.0 10.814734223616457 0.0 58-59 0.0 0.0 0.0 11.547655413691057 0.0 60-61 0.0 0.0 0.0 12.168364868448808 0.0 62-63 0.0 0.0 0.0 12.788330329641667 0.0 64-65 0.0 0.0 0.0 13.45882170675625 0.0 66-67 0.0 0.0 0.0 14.065242108635314 0.0 68-69 0.0 0.0 0.0 14.788666439675689 0.0 70-71 0.0 0.0 0.0 15.688942417523936 0.0 72-73 0.0 0.0 0.0 16.377836694287794 0.0 74-75 0.0 0.0 0.0 17.010493810411184 0.0 76-77 0.0 0.0 0.0 17.675449000116416 0.0 78-79 0.0 0.0 0.0 18.294933053708455 0.0 80-81 0.0 0.0 0.0 18.993455482488578 0.0 82-83 0.0 0.0 0.0 19.954257525057265 0.0 84-85 0.0 0.0 0.0 20.614004759808243 0.0 86-87 0.0 0.0 0.0 21.22666157833421 0.0 88-89 0.0 0.0 0.0 21.855642490960477 0.0 90-91 0.0 0.0 0.0 22.435301006667057 0.0 92-93 0.0 0.0 0.0 22.958809890387865 0.0 94-95 0.0 0.0 0.0 23.515601545055812 0.0 96-97 0.0 0.0 0.0 24.076463282166998 0.0 98-99 0.0 0.0 0.0 24.687478938417463 0.0 100-101 0.0 0.0 0.0 25.402084757497484 0.0 102-103 0.0 0.0 0.0 25.960517574440935 0.0 104-105 0.0 0.0 0.0 26.476870990640563 0.0 106-107 0.0 0.0 0.0 26.984165191079427 0.0 108-109 0.0 0.0 0.0 27.524435812174012 0.0 110-111 0.0 0.0 0.0 28.070614617460393 0.0 112-113 0.0 0.0 0.0 28.68959538128795 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 1010 0.0 88.93775 1 GTATAAC 765 0.0 83.20566 1 ATAGTAC 1090 0.0 81.77487 4 ATAACGC 795 0.0 79.978226 3 GCATAGT 1160 0.0 78.38213 2 TAGTACT 1220 0.0 72.57408 5 GTACAAC 530 0.0 71.83476 1 AGATTAC 1480 0.0 71.472984 2 AGTGTAC 910 0.0 69.871086 3 TCACGCA 460 0.0 68.46571 4 TACAACG 575 0.0 68.21183 2 CATAGTA 1335 0.0 68.10285 3 TAAACGC 530 0.0 66.150215 4 GAGTTCT 990 0.0 65.49706 1 AGTTCTT 1035 0.0 62.010757 2 GTGTACT 1055 0.0 60.26795 4 ATAAACG 575 0.0 59.939793 3 GGTTTCA 1335 0.0 59.710964 1 CGCAAAG 565 0.0 58.961716 1 TAGGACC 355 0.0 58.58613 4 >>END_MODULE