FastQCFastQC Report
Fri 10 Feb 2017
SRR3556828.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556828.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842405
Sequences flagged as poor quality0
Sequence length125
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT405952.203370051644454No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA311091.6884995427172638No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT254851.3832463546288682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204611.1105592961373856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157260.8535582567350826No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG148750.8073686295901281No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG126020.6839972753004904No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC118080.6409014304672426No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT110700.6008450910630398No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA106790.5796228299423851No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG101440.5505846977184713No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92620.5027124872110095No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC91630.49733907582751896No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC74420.40392856076704087No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70850.3845517136568778No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT62320.3382535327466002No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA61260.332500183184479No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC53230.2889158464072774No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA52030.28240262048789494No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC46980.2549927947438267No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA46800.2540158108559193No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT44250.2401752057772314No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC41710.226388877581205No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT40920.2221010038509448No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.21493645533962402No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA39470.21423085586502422No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG38230.2075005224149956No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG37340.20266987985812024No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG36470.1979477910665679No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC36370.19740502223995265No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG35780.1942026861629229No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35010.1900233661979858No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT34650.18806939842217102No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA34460.18703813765160213No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC32960.17889660525237394No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA32350.17558571541002113No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA32160.17455445463945224No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG32130.17439162399146768No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC32100.1742287933434831No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA31660.17184061050637617No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31600.17151494921040705No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.1619622178619793No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT29470.15995397320350302No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC28490.15463483870267394No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28350.15387496234541265No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG28300.15360357793210505No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA27320.14828444343127598No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC27060.14687324448207642No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT26730.14508210735424623No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA26530.1439965697010158No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC26440.1435080777570621No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG26140.14187977127721646No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG26100.14166266374657038No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC25880.1404685723280169No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT24550.13324974693403457No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA23980.13015596462232787No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT23870.12955891891305116No Hit
CATATTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA23850.1294503651477281No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT23710.12869048879046682No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA23610.1281477199638516No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC23400.12700790542795964No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA22680.12309996987633014No Hit
CACAATATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC22570.1225029241670534No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG22180.12038612574325407No Hit
CAGTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGA21440.1163696364263015No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA21420.11626108266097844No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21380.11604397513033236No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21030.11414428423717911No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT20390.11067056374684175No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20310.11023634868554959No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA20290.11012779492022655No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT19930.10817382714441179No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC19890.1079567196137657No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT19760.10725112013916593No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA19510.1058941980726279No Hit
CATGCTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAA19200.10421161471012073No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT19090.103614569000844No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19030.10328890770487488No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC18780.10193198563833684No Hit
GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA18440.10008657162784514No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA18440.10008657162784514No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9300.088.946331
TACAACG4250.078.3376852
GTACAAC4350.076.611671
ATAACGC5800.074.826383
GCATAGT11450.072.693272
TAGTACT11600.071.7493745
ATAGTAC11750.070.833434
GTATAAC6200.070.069241
CATAGTA12300.067.6679153
AGTGTAC8200.065.976213
CGCGAGT551.7258935E-764.920931
TAAACGC5150.064.64414
GAGTTCT10150.064.494521
GTGTACT8450.064.022524
GGTTTCA10250.063.8653071
GTTCAAC5050.062.4569361
AGATTAC11750.061.729622
TCACGCA4550.061.4093554
GACCGTT2850.058.4065067
AGTTCTT11450.057.6353762
Produced by FastQC (version 0.11.5)