##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3556824.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1076422 Sequences flagged as poor quality 0 Sequence length 125 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.56353549072762 33.0 33.0 34.0 32.0 34.0 2 32.61657138185581 33.0 33.0 34.0 31.0 34.0 3 32.674837563706426 33.0 33.0 34.0 32.0 34.0 4 32.602856500517454 34.0 33.0 34.0 32.0 34.0 5 32.69873339638172 34.0 33.0 34.0 32.0 34.0 6 36.09475001439956 38.0 37.0 38.0 31.0 38.0 7 36.41427432735488 38.0 37.0 38.0 34.0 38.0 8 36.62868001583022 38.0 38.0 38.0 34.0 38.0 9 36.67915743082174 38.0 38.0 38.0 34.0 38.0 10-11 36.60739932851614 38.0 38.0 38.0 34.0 38.0 12-13 36.628857920035074 38.0 38.0 38.0 34.0 38.0 14-15 36.67215181406549 38.0 38.0 38.0 34.0 38.0 16-17 36.668415361261665 38.0 38.0 38.0 34.0 38.0 18-19 36.67157583178344 38.0 38.0 38.0 34.0 38.0 20-21 36.618232904938765 38.0 38.0 38.0 34.0 38.0 22-23 36.583263812891225 38.0 38.0 38.0 34.0 38.0 24-25 36.486612592459096 38.0 38.0 38.0 34.0 38.0 26-27 36.337341674547716 38.0 38.0 38.0 33.0 38.0 28-29 36.189805206508225 38.0 38.0 38.0 33.0 38.0 30-31 36.00116032559721 38.0 38.0 38.0 32.0 38.0 32-33 35.65997954333895 38.0 38.0 38.0 30.0 38.0 34-35 35.074768538732954 38.0 37.0 38.0 26.0 38.0 36-37 34.67699052973648 38.0 36.0 38.0 24.0 38.0 38-39 34.29732530550286 38.0 35.5 38.0 15.5 38.0 40-41 33.72804671402108 38.0 34.5 38.0 15.0 38.0 42-43 33.04418434405837 38.0 33.0 38.0 15.0 38.0 44-45 32.25303366151937 38.0 27.5 38.0 15.0 38.0 46-47 31.2188193849624 38.0 24.5 38.0 14.5 38.0 48-49 30.221483303016846 38.0 15.5 38.0 14.0 38.0 50-51 29.40890793759325 37.0 15.0 38.0 14.0 38.0 52-53 28.826026874218478 36.0 15.0 38.0 14.0 38.0 54-55 28.350885154706983 35.0 15.0 38.0 14.0 38.0 56-57 27.94551765014093 34.0 15.0 38.0 14.0 38.0 58-59 27.6118901323087 33.0 15.0 38.0 14.0 38.0 60-61 27.39938100484754 31.0 15.0 38.0 14.0 38.0 62-63 27.17763293578169 28.5 15.0 38.0 14.0 38.0 64-65 26.91534686210427 26.0 15.0 38.0 14.0 38.0 66-67 26.649696401597144 26.0 15.0 38.0 14.0 38.0 68-69 26.38805087595757 26.0 15.0 38.0 14.0 38.0 70-71 26.071079929618683 25.5 14.0 38.0 14.0 38.0 72-73 25.776780853605743 25.0 14.5 38.0 14.0 38.0 74-75 25.445877639067206 25.0 14.0 38.0 14.0 38.0 76-77 25.252860402332914 24.5 14.0 38.0 13.5 38.0 78-79 24.969852901557196 23.5 14.0 38.0 13.0 38.0 80-81 24.841448799820146 23.0 14.0 38.0 13.0 38.0 82-83 24.682957984879536 23.0 14.0 38.0 13.0 38.0 84-85 24.570128629849634 22.5 14.0 38.0 13.0 38.0 86-87 24.511555876784385 22.5 13.0 38.0 13.0 38.0 88-89 24.42641779896732 22.0 13.0 38.0 13.0 38.0 90-91 24.335305762981434 21.5 13.0 38.0 13.0 38.0 92-93 24.268705024609307 21.5 13.0 38.0 13.0 38.0 94-95 24.13545245266262 21.0 13.0 38.0 13.0 38.0 96-97 24.059781851355694 21.0 13.0 38.0 13.0 38.0 98-99 23.97288191805816 21.0 13.0 38.0 13.0 38.0 100-101 23.880712675883622 21.0 13.0 38.0 13.0 38.0 102-103 23.81541207816265 21.0 13.0 38.0 13.0 38.0 104-105 23.75486426327221 21.0 13.0 38.0 13.0 38.0 106-107 23.64059402353352 21.0 13.0 38.0 13.0 38.0 108-109 23.444112067572014 21.0 13.0 38.0 13.0 38.0 110-111 23.28162653680434 21.0 13.0 37.0 13.0 38.0 112-113 23.11698060797717 21.0 13.0 36.5 13.0 38.0 114-115 22.987146769575503 21.0 13.0 36.0 13.0 38.0 116-117 22.8829004795517 21.0 13.5 35.5 13.0 38.0 118-119 22.763148189093123 21.0 14.0 35.0 13.0 38.0 120-121 22.742960939111242 21.0 13.5 35.0 13.0 38.0 122-123 22.778186900676502 21.0 13.0 35.0 13.0 38.0 124-125 22.381032253149787 18.0 14.0 34.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.27997135064219236 1101 2 -0.23165307171608163 1101 3 -0.19099758932563304 1101 4 -0.3008585189600339 1101 5 -0.04229333250124512 1101 6 0.14377589409808422 1101 7 0.04407898846539382 1101 8 0.039875998525324974 1101 9 0.19419726984762775 1101 10-11 0.19866828836043737 1101 12-13 0.2856453009826154 1101 14-15 0.19360864433190983 1101 16-17 0.14158650316989707 1101 18-19 -0.0864314041742702 1101 20-21 0.03601054077371657 1101 22-23 0.16284158477824207 1101 24-25 0.03074447689235882 1101 26-27 0.05643169861554753 1101 28-29 0.006101404616408956 1101 30-31 0.007310356421292852 1101 32-33 -0.035826469337081335 1101 34-35 -0.039517139719329464 1101 36-37 0.080235678319724 1101 38-39 0.11068246436962426 1101 40-41 0.19541333684644258 1101 42-43 0.44172947926081463 1101 44-45 0.4534156881672402 1101 46-47 0.5199983218104087 1101 48-49 0.6029651953271298 1101 50-51 0.6943746548498915 1101 52-53 0.8145797947036506 1101 54-55 1.020887131035586 1101 56-57 0.9084067709040795 1101 58-59 0.8465731919813564 1101 60-61 0.6602499120734961 1101 62-63 0.5240804543511821 1101 64-65 0.6662361206343732 1101 66-67 0.7639388431925838 1101 68-69 0.8286667996648056 1101 70-71 0.8854166145864433 1101 72-73 0.6446037499191917 1101 74-75 0.4910020995074156 1101 76-77 0.31145956495333493 1101 78-79 0.5290133490555284 1101 80-81 0.5960000777949013 1101 82-83 0.3840531423782636 1101 84-85 0.26557375895152546 1101 86-87 0.34547038492823035 1101 88-89 0.616630823689281 1101 90-91 0.5713435610601607 1101 92-93 0.2619370671146548 1101 94-95 0.4336081196513035 1101 96-97 0.46012886584051316 1101 98-99 0.5363667177054552 1101 100-101 0.42126369414052434 1101 102-103 0.34822953912880905 1101 104-105 0.5210165695271023 1101 106-107 0.3850922998418618 1101 108-109 0.14824502874292023 1101 110-111 0.20999797108028062 1101 112-113 0.18471845653578 1101 114-115 0.18773784851240904 1101 116-117 -0.0469029711013782 1101 118-119 0.10973396344801856 1101 120-121 0.18509560032445194 1101 122-123 -0.06629173845447767 1101 124-125 -0.1790903159701429 1102 1 -0.19144511762211636 1102 2 -0.3699622444493471 1102 3 -0.09613433011188732 1102 4 -0.03214473975804566 1102 5 -0.1471407724256153 1102 6 0.2848037162415409 1102 7 0.19588322539416936 1102 8 0.3084196505381982 1102 9 0.14150016141736188 1102 10-11 0.26580504776757863 1102 12-13 -0.0012775890805230006 1102 14-15 0.15202101063183449 1102 16-17 0.10525926986965572 1102 18-19 -0.0023174748202237083 1102 20-21 0.14173739437715227 1102 22-23 -0.06966557368296122 1102 24-25 0.049489784640599055 1102 26-27 0.002914847982985691 1102 28-29 -0.09216439837969403 1102 30-31 -0.0639703966073526 1102 32-33 -0.12704130442797634 1102 34-35 -0.20264118275389364 1102 36-37 -0.09620102886430004 1102 38-39 -0.1411870303018361 1102 40-41 -0.11073858080861498 1102 42-43 0.05735587318228852 1102 44-45 -0.06660811271115818 1102 46-47 0.10359806077944 1102 48-49 0.14302422353692634 1102 50-51 0.03122268048577226 1102 52-53 -0.1113107703471492 1102 54-55 -0.09337401335330853 1102 56-57 -0.2033045816578607 1102 58-59 -0.25408581811539577 1102 60-61 -0.23836570966520298 1102 62-63 -0.08991068718533413 1102 64-65 -0.1699123521567074 1102 66-67 -0.11052210330706913 1102 68-69 0.013678848224692786 1102 70-71 -0.09515249048529384 1102 72-73 -0.17225983262084554 1102 74-75 -0.045627723196354 1102 76-77 -0.12394204101347484 1102 78-79 -0.11003727351687687 1102 80-81 0.18082033122516705 1102 82-83 0.1382862200129722 1102 84-85 0.008963979092722951 1102 86-87 0.08913864875910704 1102 88-89 -0.02562005212718077 1102 90-91 0.15125424851789404 1102 92-93 -0.02922842262735159 1102 94-95 0.14873648950273477 1102 96-97 0.04322499447360073 1102 98-99 -0.02436318718413588 1102 100-101 0.09082717824379216 1102 102-103 0.05364584713181131 1102 104-105 0.029847524888602095 1102 106-107 -0.07800702819805849 1102 108-109 -0.10644426060787993 1102 110-111 0.02734527346400384 1102 112-113 -0.08790063038059515 1102 114-115 0.08214684273908546 1102 116-117 -0.01839100845149133 1102 118-119 0.2574750383302238 1102 120-121 0.16723182226510502 1102 122-123 0.1640829053152686 1102 124-125 -0.09027122719167835 1103 1 -0.14547367702068925 1103 2 0.012024524709048023 1103 3 0.08268376716149817 1103 4 0.01619144382302551 1103 5 0.001957737325916753 1103 6 0.1720812315956266 1103 7 0.277612091079277 1103 8 0.21981670720748525 1103 9 0.3744679431545137 1103 10-11 0.29480181916097337 1103 12-13 0.4725574715615366 1103 14-15 0.2883992958457853 1103 16-17 0.2519532754763958 1103 18-19 0.2500826346694609 1103 20-21 0.2790678548616654 1103 22-23 0.1458202700866309 1103 24-25 0.27751964820539143 1103 26-27 0.004159163182713144 1103 28-29 0.2629622388385968 1103 30-31 0.10132597474686378 1103 32-33 0.15146682317272564 1103 34-35 0.15693075272755408 1103 36-37 0.1192341717370553 1103 38-39 0.133198073742669 1103 40-41 -0.14371356140935632 1103 42-43 -0.0657036773240236 1103 44-45 0.19475667463357382 1103 46-47 0.37181223199352686 1103 48-49 0.2715297980522813 1103 50-51 0.362706491832018 1103 52-53 0.13540516668121327 1103 54-55 0.14861285625434562 1103 56-57 0.1331740691076888 1103 58-59 0.05469646062627476 1103 60-61 0.05899297706118034 1103 62-63 0.08383346687704574 1103 64-65 0.019722874728874018 1103 66-67 -3.1294283686733593E-4 1103 68-69 0.01183937116353917 1103 70-71 0.19229394703987523 1103 72-73 0.040416780196647295 1103 74-75 0.14434484578508133 1103 76-77 -0.11120465639409716 1103 78-79 0.08794528681755409 1103 80-81 -0.12270635856157242 1103 82-83 -0.04015602961515441 1103 84-85 0.1515236606016046 1103 86-87 -0.1318643705782101 1103 88-89 -0.21911713449473424 1103 90-91 -0.0714505486876078 1103 92-93 -0.11615763017477576 1103 94-95 -0.07594422834115377 1103 96-97 0.03937025414748874 1103 98-99 0.14970700843242213 1103 100-101 -0.336856888745821 1103 102-103 0.07578235265275524 1103 104-105 0.3011015418126277 1103 106-107 0.2360454883488643 1103 108-109 0.12378544173585126 1103 110-111 0.24083175843470528 1103 112-113 0.502314523562081 1103 114-115 0.39159459782370476 1103 116-117 0.3130296035691025 1103 118-119 0.5087635093501923 1103 120-121 0.4662177869923454 1103 122-123 0.30214957516282936 1103 124-125 0.29461099186639217 1104 1 -0.06399356814555546 1104 2 -0.10400985084428527 1104 3 -0.009720915622310144 1104 4 -0.05585391807150586 1104 5 -0.16761487423609367 1104 6 0.3096452122089346 1104 7 0.28713331225969085 1104 8 0.20895719483961273 1104 9 0.22573396661784528 1104 10-11 0.23896707547461205 1104 12-13 0.38054809940540224 1104 14-15 0.28102469373005334 1104 16-17 0.14914986427510968 1104 18-19 0.06712793479670864 1104 20-21 0.0627069362585928 1104 22-23 0.15601228398839595 1104 24-25 0.06811743199518361 1104 26-27 0.14020284500707447 1104 28-29 0.040437356917060185 1104 30-31 0.13298706176873054 1104 32-33 0.10375283041184957 1104 34-35 0.12075241822063987 1104 36-37 0.27431906057653066 1104 38-39 0.13913843948735405 1104 40-41 0.32338424091062734 1104 42-43 0.6746443607410981 1104 44-45 0.5054465328570643 1104 46-47 0.5674395199567321 1104 48-49 0.5850683634864815 1104 50-51 0.4475067981977574 1104 52-53 0.496074229918559 1104 54-55 0.8159951998698745 1104 56-57 0.6197162129266403 1104 58-59 0.6076013760668637 1104 60-61 0.5975283511856304 1104 62-63 0.47144212582691836 1104 64-65 0.62019642391828 1104 66-67 0.5727379904668162 1104 68-69 0.5048287842507904 1104 70-71 0.39402631350929695 1104 72-73 0.569456875400622 1104 74-75 0.6645917502146226 1104 76-77 0.3266545718442586 1104 78-79 0.29356652158676866 1104 80-81 0.6458125031085338 1104 82-83 0.3198809483998062 1104 84-85 0.28637935163340345 1104 86-87 0.42737869192616174 1104 88-89 0.4695514510756027 1104 90-91 0.5042205528701622 1104 92-93 0.6594471744964778 1104 94-95 0.6812691611592285 1104 96-97 0.5577497276431451 1104 98-99 0.5164300892199165 1104 100-101 0.4532109317337394 1104 102-103 0.3666593245733445 1104 104-105 0.499452767069684 1104 106-107 0.3232197173901916 1104 108-109 0.35443436883810264 1104 110-111 0.44160961445104263 1104 112-113 0.29366916249111696 1104 114-115 0.5156900159264595 1104 116-117 0.36182122957231044 1104 118-119 0.17510322118521415 1104 120-121 0.364572306504634 1104 122-123 0.2770444678318782 1104 124-125 -0.02507630095583835 1105 1 0.06699687833523882 1105 2 0.058719238646844474 1105 3 0.1392449321712803 1105 4 -0.08382801958576636 1105 5 -0.051871064526601174 1105 6 0.24328237344894177 1105 7 0.27525152893610993 1105 8 0.2499300940402378 1105 9 0.33636956809961305 1105 10-11 0.3276775128568232 1105 12-13 0.2837037665270046 1105 14-15 0.24377693588251503 1105 16-17 0.25565007549236896 1105 18-19 0.04279733104028338 1105 20-21 0.12561759135705586 1105 22-23 0.08328408810874777 1105 24-25 0.18781953441931165 1105 26-27 0.19222809359354187 1105 28-29 0.19665689288898847 1105 30-31 0.1827965600464978 1105 32-33 0.1580506954927614 1105 34-35 0.2704660563567245 1105 36-37 0.49434895594275474 1105 38-39 0.6702783627992446 1105 40-41 0.5201907983942107 1105 42-43 0.5298516786068319 1105 44-45 0.19886020998711018 1105 46-47 -0.04020816965137897 1105 48-49 0.08770289263446429 1105 50-51 0.03389465807947545 1105 52-53 0.1704243306529456 1105 54-55 0.022322781594706242 1105 56-57 0.05468038722962021 1105 58-59 0.13683953165274687 1105 60-61 -0.009342302613035258 1105 62-63 -0.0383880067525304 1105 64-65 -0.0157105105068851 1105 66-67 0.012255927686549484 1105 68-69 0.051668242884701954 1105 70-71 0.11859250574950053 1105 72-73 -0.0975014335614901 1105 74-75 -0.11125961782333249 1105 76-77 -0.1983982324710709 1105 78-79 -0.07218866135987767 1105 80-81 -0.24843873170007313 1105 82-83 0.016146870931816437 1105 84-85 -0.16508860833279826 1105 86-87 0.07444277199375549 1105 88-89 0.16018477790994368 1105 90-91 0.09285056521824941 1105 92-93 0.25373821563509935 1105 94-95 -0.009354417186127506 1105 96-97 0.09240804310425332 1105 98-99 0.17118288378675572 1105 100-101 0.1793389131466192 1105 102-103 0.07881040990512034 1105 104-105 0.19144519574323837 1105 106-107 0.29297096844628 1105 108-109 0.22880924698199223 1105 110-111 0.14176924595815876 1105 112-113 0.39831878554954514 1105 114-115 0.31364364561385827 1105 116-117 0.07576441212900775 1105 118-119 0.20210003667881438 1105 120-121 0.3400499824648584 1105 122-123 0.13651471869299314 1105 124-125 0.17569829773136547 1106 1 -0.03725428727637592 1106 2 -0.04075339317151361 1106 3 0.062034218652712525 1106 4 -0.06875167044560726 1106 5 -0.1116584114808461 1106 6 -0.05981929062748037 1106 7 0.14595550780979494 1106 8 0.11666183279161402 1106 9 0.08841649581274424 1106 10-11 0.00797220095305562 1106 12-13 0.11878354300754523 1106 14-15 0.12131071966630458 1106 16-17 0.14007960495170835 1106 18-19 0.030356243388823145 1106 20-21 0.05906045743491006 1106 22-23 -0.026967687158801823 1106 24-25 0.1776255659066166 1106 26-27 0.06669503950158173 1106 28-29 0.15362402832736421 1106 30-31 0.07253925681058604 1106 32-33 0.03961436821175823 1106 34-35 0.2522746970451024 1106 36-37 0.30327078099360705 1106 38-39 0.12197023700164067 1106 40-41 0.13397455058960617 1106 42-43 -0.07836856828802752 1106 44-45 -0.1430371309021652 1106 46-47 -0.011186335755770216 1106 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-0.042091229887681436 2313 38-39 0.09546646561194905 2313 40-41 0.023015496672257996 2313 42-43 0.04141558306574922 2313 44-45 -0.13748560882822147 2313 46-47 -0.16380686681847223 2313 48-49 0.10011578462052739 2313 50-51 -0.14667141776761738 2313 52-53 -0.06877553805381709 2313 54-55 0.16106754248951916 2313 56-57 0.12137009333475746 2313 58-59 -0.21281392994398018 2313 60-61 -0.06322460579941946 2313 62-63 0.027676794035226493 2313 64-65 0.187377405465444 2313 66-67 0.24026115416738492 2313 68-69 0.12056721021288652 2313 70-71 -0.0530670912812532 2313 72-73 0.22927039393939452 2313 74-75 0.37767513953665954 2313 76-77 0.31225987488639717 2313 78-79 0.2799612654188941 2313 80-81 -0.0029156417642006716 2313 82-83 -0.03772596399218742 2313 84-85 -0.03438555828987688 2313 86-87 0.012242600302595719 2313 88-89 0.1882992050169463 2313 90-91 0.14050748588683248 2313 92-93 0.0995238920477668 2313 94-95 0.13017620948659925 2313 96-97 -0.055741858425822244 2313 98-99 0.27597603381452984 2313 100-101 0.1152876961592959 2313 102-103 0.08996560103777895 2313 104-105 0.19468018539127385 2313 106-107 0.38696872131200877 2313 108-109 0.293084546179081 2313 110-111 0.1921072629252869 2313 112-113 0.3205859294166338 2313 114-115 0.22533947452866698 2313 116-117 0.17272730916481294 2313 118-119 0.4103702482629181 2313 120-121 0.37913496958802284 2313 122-123 0.5356982388667006 2313 124-125 0.34603391607202383 2314 1 -0.009090078186495987 2314 2 -0.07791119613576569 2314 3 -0.10426956058234538 2314 4 0.01417922679104322 2314 5 -0.07926236887442428 2314 6 -0.1538915395945395 2314 7 -0.600762963817516 2314 8 -0.3948525146554118 2314 9 -0.38667391016125663 2314 10-11 -0.5888804581576679 2314 12-13 -0.42098826245850063 2314 14-15 -0.15595132498705055 2314 16-17 -0.28248398247864515 2314 18-19 -0.1801736834524661 2314 20-21 -0.26661066951250945 2314 22-23 -0.2793064916013961 2314 24-25 -0.14281452355169932 2314 26-27 -0.11135886292819919 2314 28-29 0.13491776245420084 2314 30-31 0.18930018323490572 2314 32-33 0.14908330418840876 2314 34-35 -0.2751317697302298 2314 36-37 0.02724750666738629 2314 38-39 0.06607546424852018 2314 40-41 -0.04875847696810354 2314 42-43 0.05992414237494614 2314 44-45 0.13315368824797957 2314 46-47 0.42435704773992455 2314 48-49 0.3721594143735949 2314 50-51 0.2659236435867207 2314 52-53 0.4507866494935264 2314 54-55 0.5245329265222409 2314 56-57 0.4360390828817948 2314 58-59 0.2730351838266607 2314 60-61 0.4322700162275481 2314 62-63 0.3677532975952964 2314 64-65 0.22153586630471267 2314 66-67 0.31866897116480786 2314 68-69 0.3296211414354282 2314 70-71 0.11424467966254426 2314 72-73 0.3109405954240181 2314 74-75 0.16750849811869628 2314 76-77 0.18337712984776644 2314 78-79 0.23208670095896622 2314 80-81 0.057304021923119564 2314 82-83 0.07808527672891685 2314 84-85 0.19248385543531654 2314 86-87 0.3239355256169425 2314 88-89 0.3146050677650116 2314 90-91 0.23792302898636564 2314 92-93 0.0506403895481462 2314 94-95 0.3962252929587997 2314 96-97 0.3025167387564274 2314 98-99 0.22946186929400625 2314 100-101 0.29703818850894237 2314 102-103 0.12618359831091652 2314 104-105 0.17082925371425617 2314 106-107 0.14865937424338327 2314 108-109 0.045276638286335213 2314 110-111 0.11171489813207458 2314 112-113 0.039822408737951065 2314 114-115 0.0710711818457419 2314 116-117 0.13871731067973414 2314 118-119 -0.01645068795886573 2314 120-121 -0.3588630610134018 2314 122-123 -0.33611209290121025 2314 124-125 -0.18562416603675302 2315 1 0.2900403565961085 2315 2 0.14034967342945492 2315 3 -0.03296521275625253 2315 4 0.08374444418235072 2315 5 0.007694152864701209 2315 6 -0.11650023524671127 2315 7 -0.16250209425229656 2315 8 -0.3122438190032426 2315 9 -0.07015217103081994 2315 10-11 -0.25975002337505515 2315 12-13 -0.39664043637154833 2315 14-15 -0.05942958585661273 2315 16-17 -0.33422311291342055 2315 18-19 -0.2040867269307256 2315 20-21 -0.03748023472989814 2315 22-23 -0.20365431768835407 2315 24-25 -6.406105082206182E-4 2315 26-27 0.04994548489788997 2315 28-29 0.2370916754976733 2315 30-31 0.24060453106099544 2315 32-33 0.2512572172318883 2315 34-35 0.026172578095852828 2315 36-37 -0.03666553681087237 2315 38-39 -0.3160984487949605 2315 40-41 -0.48875847696810126 2315 42-43 -0.34964107501635766 2315 44-45 -0.32336805088245413 2315 46-47 -0.1469473000861612 2315 48-49 -0.012623194322056719 2315 50-51 -0.11059809554371114 2315 52-53 -0.10877856789777951 2315 54-55 0.0023590134787667694 2315 56-57 0.004734735055706096 2315 58-59 0.13303518382666013 2315 60-61 0.1792265379666773 2315 62-63 0.13253590629094703 2315 64-65 0.2237097793481908 2315 66-67 0.029973318990897724 2315 68-69 0.2983167936093416 2315 70-71 0.39598381009732364 2315 72-73 -0.2221028828368503 2315 74-75 -0.16727411057695818 2315 76-77 0.020768434195591823 2315 78-79 0.049912787915488366 2315 80-81 0.08643445670572802 2315 82-83 0.05417223325065734 2315 84-85 0.14074472500053403 2315 86-87 0.0017616125734640775 2315 88-89 -0.10191667136541938 2315 90-91 0.048357811595060696 2315 92-93 0.011944737374232517 2315 94-95 -0.15029644617163385 2315 96-97 -0.08096152211313878 2315 98-99 0.2651140432070491 2315 100-101 0.3092121015524185 2315 102-103 0.03183577222396039 2315 104-105 0.14822055806208212 2315 106-107 0.24865937424338114 2315 108-109 0.11658098611242451 2315 110-111 0.10388881117555115 2315 112-113 0.13286588699881818 2315 114-115 0.16194074706313444 2315 116-117 -0.08084790671156838 2315 118-119 0.12746235551939478 2315 120-121 -0.1649500175351406 2315 122-123 0.005627037533571411 2315 124-125 0.05611496439803432 2316 1 0.14511083675380831 2316 2 0.039916006601615095 2316 3 0.08043010840266618 2316 4 0.10408884854133049 2316 5 0.10408720526920945 2316 6 -0.22863249247139095 2316 7 -0.19678111385285035 2316 8 -0.18311375614758418 2316 9 -0.15475067588446478 2316 10-11 -0.31178037261447145 2316 12-13 -0.28404698972446596 2316 14-15 -0.061959804921592365 2316 16-17 -0.0887081800462397 2316 18-19 -0.28441625421157113 2316 20-21 -0.31760892145582886 2316 22-23 -0.2095088198641193 2316 24-25 0.010635470869388541 2316 26-27 -0.025053957211682132 2316 28-29 0.23452835493383617 2316 30-31 0.18447294330482578 2316 32-33 0.1196155316589369 2316 34-35 -0.17840212343276107 2316 36-37 -0.2719755379266502 2316 38-39 -0.1572250155372501 2316 40-41 0.1152561025684804 2316 42-43 0.07883290869324355 2316 44-45 0.3575695769674354 2316 46-47 -0.014252317380766044 2316 48-49 0.12379142984575608 2316 50-51 0.21097218005415996 2316 52-53 0.049884354704222034 2316 54-55 0.25545976849047847 2316 56-57 0.3391870726166104 2316 58-59 0.28408773710519597 2316 60-61 0.25272711445345664 2316 62-63 0.2998528252480632 2316 64-65 0.1858491031849887 2316 66-67 0.029631890047543408 2316 68-69 -0.04018880389131141 2316 70-71 0.21386159490038992 2316 72-73 0.1951965555534514 2316 74-75 0.3017451180465436 2316 76-77 0.31032732882868785 2316 78-79 0.43151356152354836 2316 80-81 0.4612866157416917 2316 82-83 0.16857919572322544 2316 84-85 0.09773434823852867 2316 86-87 -0.07506101546239208 2316 88-89 0.15270749644801995 2316 90-91 0.14546273218865835 2316 92-93 0.14142441156622 2316 94-95 -0.03467179485314986 2316 96-97 -0.17509809369048313 2316 98-99 0.08251278609394319 2316 100-101 0.08332635751254713 2316 102-103 -0.13836432447704894 2316 104-105 -0.1293146820167692 2316 106-107 -0.12935751124031114 2316 108-109 -0.20993554604065778 2316 110-111 -0.19026040591821314 2316 112-113 -0.0157866216484841 2316 114-115 0.15622943676447676 2316 116-117 0.1259602161730946 2316 118-119 -0.13891358824524858 2316 120-121 -0.09911671519571996 2316 122-123 -0.39895510253411715 2316 124-125 -0.25770546926879234 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 1.0 13 8.0 14 104.0 15 745.0 16 1226.0 17 1629.0 18 3195.0 19 8115.0 20 22945.0 21 51964.0 22 82377.0 23 120492.0 24 134229.0 25 91777.0 26 50241.0 27 37039.0 28 22860.0 29 17303.0 30 16126.0 31 17334.0 32 20939.0 33 27904.0 34 40605.0 35 51888.0 36 76220.0 37 179155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 59.75454839657629 14.15694246571266 10.91159501694373 15.176914120767323 2 14.545275493623516 27.178488577886746 38.472393966054646 19.803841962435094 3 22.28350692170268 41.5782466877927 23.96690068812901 12.17134570237561 4 12.781232639243717 18.164623168236993 32.78388958977055 36.270254602748736 5 11.22450117147364 41.9090282435699 27.970164117790237 18.896306467166223 6 24.271521763769226 31.66648396260946 24.4125445225014 19.649449751119914 7 23.771346182073575 35.747225530507556 23.7179284704326 16.763499816986275 8 31.239885472426245 22.23914041147431 25.91297836722029 20.60799574887916 9 36.03577407373688 12.49937292251552 23.785281237284263 27.67957176646334 10-11 22.151906965855396 27.91804701130226 27.174936967100265 22.75510905574208 12-13 26.520268073302105 26.71419759165086 32.127130437690795 14.638403897356241 14-15 25.37513168627174 22.506693471519533 36.89881849311887 15.219356349089855 16-17 15.662258853869579 27.32079054497214 36.18831647810989 20.828634123048396 18-19 16.456185399406554 23.913437295038563 45.006976817642155 14.623400487912733 20-21 19.750772478042673 24.017842709906855 43.21362013961274 13.017764672437723 22-23 16.842806419067685 28.372760396054318 39.62062135713078 15.163811827747214 24-25 15.13211851982649 30.03334038089352 40.69278291113801 14.141758188141976 26-27 16.47302261916888 28.730153636215228 40.971192221309074 13.825631523306816 28-29 15.972381289117873 29.86267306413717 41.87897741448835 12.285968232256614 30-31 17.186454569784555 30.593655678962783 40.36703428639398 11.852855464858676 32-33 12.875360220840983 32.01464901958143 41.08308528830908 14.026905471268512 34-35 11.559845311679826 33.91764519030268 39.68441011687324 14.838099381144252 36-37 13.632666794936501 34.84152633667858 37.37397256326817 14.15183430511676 38-39 13.97230290215396 33.7339498570997 37.217882287334156 15.07586495341218 40-41 12.220799161282168 35.60995717185536 36.64673658460417 15.522507082258292 42-43 13.556938080508305 35.02470376622659 35.944084408677845 15.474273744587267 44-45 13.974743118856786 33.60793469047867 35.97576729765498 16.44155489300956 46-47 12.352509002463393 33.09356195493215 37.08842977242088 17.46549927018357 48-49 12.445920940248389 32.54887910547775 36.81302748865908 18.19217246561478 50-51 11.241852845840159 31.291717495614186 36.90713696513425 20.559292693411408 52-53 11.710385662895865 29.675779180338534 36.379100398769296 22.2347347579963 54-55 12.588983994151468 28.16395409850389 36.754334229854194 22.49272767749045 56-57 13.524795489380134 25.652663308735978 37.53175363731932 23.290787564564567 58-59 13.012628412720249 25.45128031618437 38.595528281472504 22.940562989622876 60-61 13.730558027881642 24.990995052507557 38.53294881239266 22.745498107218136 62-63 12.196096840011341 25.452711715522007 39.7602238344666 22.590967610000046 64-65 11.556906098917901 25.471912963698003 39.76083422183511 23.210346715548983 66-67 12.812083890083075 24.50510660547261 39.81785859524778 22.864950909196537 68-69 13.269547249556274 23.55118417267324 39.88225895129878 23.297009626471706 70-71 12.589853329993339 23.921306910207317 39.379542046037066 24.109297713762274 72-73 13.583080031577097 23.581796863304454 38.300480816169404 24.534642288949037 74-75 13.928989610559848 22.76761372906575 37.859424845873626 25.443971814500777 76-77 13.357086497567572 22.983999393694514 36.65284035985905 27.00607374887887 78-79 13.874286787188034 23.318277070251664 34.753755971421754 28.053680171138545 80-81 13.51857006696944 23.903457307271744 32.755961423445676 29.822011202313142 82-83 13.742352439900992 23.91609905782896 30.49512651725687 31.846421985013173 84-85 14.756285779860418 23.577934935825066 28.88838402563095 32.77739525868357 86-87 15.523332035637996 23.08209765792317 27.658610735068528 33.7359595713703 88-89 15.832306700336513 23.70825412929444 26.795818026746343 33.6636211436227 90-91 16.55615816962249 25.044530154603496 25.100372873261183 33.29893880251283 92-93 16.103495995425202 26.71755867035388 23.933704439570892 33.245240894650024 94-95 15.860654595453322 27.728332667527567 23.96552364849172 32.44548908852739 96-97 16.710740170091363 28.393059781879128 23.377877532174896 31.518322515854617 98-99 17.295744579054233 29.354884111766637 23.020549450090044 30.32882185908909 100-101 16.99994983333519 30.077423886021336 23.30529574177917 29.6173305388643 102-103 16.641884399899848 31.385312453983254 23.570699603158566 28.40210354295833 104-105 16.43753977359823 31.4411861706328 24.501511977369113 27.619762078399855 106-107 15.158526366249417 32.05174774761383 25.813999074705457 26.97572681143129 108-109 14.784088649906076 32.241498159630034 26.830522456972876 26.143890733491016 110-111 14.198255143665909 32.03356307615701 28.158592223674567 25.60958955650252 112-113 13.488730088832652 31.97469170554605 28.84469243017969 25.69188577544161 114-115 13.588245322363143 31.288825465928817 29.867894123915395 25.255035087792642 116-117 13.651845898699719 31.09643641904479 30.83252423801803 24.41919344423746 118-119 13.125064746169466 32.108479042216594 30.86721077450587 23.899245437108068 120-121 12.880455989743018 33.021257223553896 31.45339762528569 22.644889161417396 122-123 11.936390904306245 34.53394347871126 31.35808754635434 22.171578070628154 124-125 10.862611645921474 36.28030256578911 32.10474045749047 20.752345330798946 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4.0 1 2.0 2 11.5 3 52.5 4 107.0 5 192.0 6 401.5 7 708.0 8 1123.5 9 1670.0 10 2160.0 11 2605.5 12 3128.5 13 3769.5 14 4458.0 15 5170.5 16 6023.0 17 7101.0 18 8340.0 19 9680.0 20 11195.5 21 12890.5 22 15075.0 23 17445.0 24 20004.0 25 22875.5 26 26279.5 27 30177.0 28 34050.5 29 37884.5 30 41367.5 31 43682.5 32 43989.0 33 42057.0 34 38186.0 35 33006.0 36 27664.5 37 22786.5 38 18306.0 39 14408.5 40 11295.0 41 9016.0 42 7493.5 43 7159.5 44 7733.5 45 8840.5 46 11908.5 47 16437.0 48 27357.0 49 41139.0 50 61978.0 51 67184.5 52 45035.0 53 31492.5 54 28588.5 55 24600.5 56 18940.5 57 13497.5 58 9198.5 59 6307.5 60 3878.5 61 2633.0 62 1915.0 63 1053.5 64 449.5 65 197.5 66 148.5 67 126.0 68 127.5 69 122.0 70 91.0 71 80.0 72 80.0 73 71.0 74 51.5 75 43.0 76 31.5 77 19.5 78 17.0 79 12.0 80 12.0 81 13.0 82 7.0 83 1.0 84 0.5 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10200460414224162 2 0.004180516563206623 3 0.0023225092017814574 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0014864058891401327 22-23 3.716014722850332E-4 24-25 0.037113697044467694 26-27 0.1284347588585146 28-29 0.024850848459061593 30-31 0.028799114102090073 32-33 0.016025313492292057 34-35 0.2702007205352548 36-37 0.052488707960260936 38-39 0.046171482931415375 40-41 0.0458927818272016 42-43 0.03451248673847246 44-45 0.040690361215211135 46-47 0.043338021705241994 48-49 0.04189806600013749 50-51 0.0473327375323061 52-53 0.05685502525961007 54-55 0.05541506955450558 56-57 0.052117106487975906 58-59 0.05518281863432743 60-61 0.0580162798605008 62-63 0.05824853078067895 64-65 0.05954913593367657 66-67 0.05662277433943193 68-69 0.054486065873792996 70-71 0.053092560352724125 72-73 0.08909145298033672 74-75 0.07083653065433446 76-77 0.0981492388672844 78-79 0.05281385924851034 80-81 0.04491732796245339 82-83 0.03191127643247722 84-85 0.0207167820798906 86-87 0.014399557051045037 88-89 0.00817523239027073 90-91 0.017186568093182784 92-93 0.008407483310448877 94-95 0.0029728117782802654 96-97 4.645018403562915E-5 98-99 0.0017186568093182785 100-101 9.29003680712583E-4 102-103 0.0044127674833847694 104-105 0.0015793062572113912 106-107 7.432029445700664E-4 108-109 7.432029445700664E-4 110-111 9.29003680712583E-5 112-113 0.0014864058891401327 114-115 0.004691468587598544 116-117 0.010869343064337221 118-119 0.009615188095375234 120-121 0.007617830181843181 122-123 0.006177874476738676 124-125 0.0026012103059952323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1076422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.299085028652204 #Duplication Level Percentage of deduplicated Percentage of total 1 78.45358101814655 22.20164560034784 2 9.902180086538245 5.604452724759448 3 3.7762981073235045 3.2059734369805866 4 1.9148757232186193 2.1675692364266235 5 1.0907228857384095 1.5433229843104077 6 0.7398771069956156 1.2562707096973278 7 0.5315355057101534 1.0529377930287498 8 0.3871197200872868 0.8764107100014537 9 0.32758744754597174 0.8343382529180339 >10 2.2741533557664164 12.822530328073928 >50 0.30301937115004823 5.9752839073269755 >100 0.24049680462767165 13.732255680750288 >500 0.032749908745721194 6.434186388375079 >1k 0.022825693974290528 11.530795952698025 >5k 0.001654035795238444 3.276726203490536 >10k+ 0.001323228636190755 7.485300090814703 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35133 3.2638686314475174 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18050 1.6768516436862124 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14235 1.322436739494362 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 12540 1.164970615613579 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 9689 0.9001116662424216 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7838 0.7281530849425225 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6279 0.5833214111194309 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 5969 0.5545222970173408 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 5227 0.48559022390846707 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 4637 0.4307790067464247 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 3908 0.3630546384224774 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 3903 0.36259013658212114 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 3554 0.330167908125252 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3484 0.3236648823602639 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3459 0.32134237315848246 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 3297 0.3062925135309386 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 3103 0.28826984212511453 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 3061 0.28436802666612165 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2842 0.26402284605851606 No Hit GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2735 0.25408250667489146 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 2725 0.2531535029941789 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 2713 0.25203869857732375 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 2619 0.24330606397862548 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 2543 0.23624563600520987 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2471 0.22955680950407925 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2273 0.21116253662597012 No Hit GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 2021 0.187751643872013 No Hit GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA 1979 0.1838498284130202 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA 1939 0.18013381369016984 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1918 0.17818290596067343 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 1894 0.1759532971269632 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 1870 0.17372368829325302 No Hit GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1807 0.16787096510476374 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 1740 0.16164664044398944 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 1730 0.16071763676327686 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 1621 0.1505914966435097 No Hit GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1558 0.14473877345502043 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA 1557 0.14464587308694918 No Hit GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA 1497 0.13907185100267366 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1438 0.13359072928646945 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 1429 0.1327546259738281 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 1404 0.13043211677204664 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 1387 0.12885281051483527 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA 1384 0.12857410941062147 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1382 0.12838830867447898 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 1362 0.12653030131305382 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 1348 0.1252296961600562 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA 1347 0.12513679579198494 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 1313 0.12197818327756216 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 1308 0.12151368143720587 No Hit GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA 1281 0.11900537149928188 No Hit GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 1270 0.11798346745049804 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAACACAGGTGGAAAA 1265 0.11751896561014175 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 1255 0.11658996192942916 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 1231 0.11436035309571896 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT 1230 0.1142674527276477 No Hit CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC 1216 0.11296684757465009 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1215 0.11287394720657883 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 1202 0.11166624242165248 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 1196 0.11110884021322492 No Hit CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1163 0.10804312806687341 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA 1149 0.10674252291387577 No Hit GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1135 0.10544191776087816 No Hit GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA 1123 0.10432711334402307 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 1122 0.10423421297595181 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 1098 0.10200460414224162 No Hit GGTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAA 1080 0.10033239751695897 No Hit GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1077 0.1000536964127452 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.29003680712583E-5 0.0 5 0.0 0.0 0.0 2.787011042137749E-4 0.0 6 0.0 0.0 0.0 2.787011042137749E-4 0.0 7 0.0 0.0 0.0 2.787011042137749E-4 0.0 8 0.0 0.0 0.0 2.787011042137749E-4 0.0 9 0.0 0.0 0.0 3.716014722850332E-4 0.0 10-11 9.29003680712583E-5 0.0 0.0 8.825534966769538E-4 0.0 12-13 9.29003680712583E-5 0.0 0.0 0.001904457545460795 0.0 14-15 9.29003680712583E-5 0.0 0.0 0.0019973579135320533 0.0 16-17 9.29003680712583E-5 0.0 0.0 0.0036695645388147026 0.0 18-19 9.29003680712583E-5 0.0 0.0 0.007478479629736292 0.0 20-21 9.29003680712583E-5 0.0 0.0 0.015328560731757618 0.0 22-23 9.29003680712583E-5 0.0 0.0 0.04919074489373127 0.0 24-25 9.29003680712583E-5 0.0 0.0 0.1225355854859897 0.0 26-27 9.29003680712583E-5 0.0 0.0 0.22444728926016005 0.0 28-29 9.29003680712583E-5 0.0 0.0 0.4478726744715362 0.0 30-31 9.29003680712583E-5 0.0 0.0 0.9623084626661291 0.0 32-33 9.29003680712583E-5 0.0 0.0 1.8403562914916267 0.0 34-35 9.29003680712583E-5 0.0 0.0 2.4980444472521 0.0 36-37 9.29003680712583E-5 0.0 0.0 3.2322360561192545 0.0 38-39 9.29003680712583E-5 0.0 0.0 3.9958770816649976 0.0 40-41 9.29003680712583E-5 0.0 0.0 5.207112080578064 0.0 42-43 9.29003680712583E-5 0.0 0.0 6.18145114090942 0.0 44-45 9.29003680712583E-5 0.0 0.0 6.931482262532724 0.0 46-47 9.29003680712583E-5 0.0 0.0 7.627538270306627 0.0 48-49 9.29003680712583E-5 0.0 0.0 8.218291710871757 0.0 50-51 9.29003680712583E-5 0.0 0.0 8.788932221749462 0.0 52-53 9.29003680712583E-5 0.0 0.0 9.519361365709731 0.0 54-55 9.29003680712583E-5 0.0 0.0 10.136916562463421 0.0 56-57 9.29003680712583E-5 0.0 0.0 10.643409369187921 0.0 58-59 9.29003680712583E-5 0.0 0.0 11.204620492706393 0.0 60-61 9.29003680712583E-5 0.0 0.0 11.650077757608075 0.0 62-63 9.29003680712583E-5 0.0 0.0 12.112117738210479 0.0 64-65 9.29003680712583E-5 0.0 0.0 12.633149452538131 0.0 66-67 9.29003680712583E-5 0.0 0.0 13.096211337189319 0.0 68-69 9.29003680712583E-5 0.0 0.0 13.58853683778295 0.0 70-71 9.29003680712583E-5 0.0 0.0 14.220677392323829 0.0 72-73 9.29003680712583E-5 0.0 0.0 14.699392988995022 0.0 74-75 1.858007361425166E-4 0.0 0.0 15.127338534515275 0.0 76-77 1.858007361425166E-4 0.0 0.0 15.571680995000102 0.0 78-79 1.858007361425166E-4 0.0 0.0 15.975797596110077 0.0 80-81 1.858007361425166E-4 0.0 0.0 16.421347761379828 0.0 82-83 1.858007361425166E-4 0.0 0.0 17.022413142800872 0.0 84-85 1.858007361425166E-4 0.0 0.0 17.456490112613828 0.0 86-87 1.858007361425166E-4 0.0 0.0 17.85368563630249 0.0 88-89 1.858007361425166E-4 0.0 0.0 18.24563228919513 0.0 90-91 1.858007361425166E-4 0.0 0.0 18.601394248724013 0.0 92-93 1.858007361425166E-4 0.0 0.0 18.913632385811514 0.0 94-95 1.858007361425166E-4 0.0 0.0 19.249327865837003 0.0 96-97 1.858007361425166E-4 0.0 0.0 19.591201220339236 0.0 98-99 1.858007361425166E-4 0.0 0.0 19.952444301584322 0.0 100-101 1.858007361425166E-4 0.0 0.0 20.3990628210869 0.0 102-103 1.858007361425166E-4 0.0 0.0 20.748646906139044 0.0 104-105 1.858007361425166E-4 0.0 0.0 21.055729072798584 0.0 106-107 1.858007361425166E-4 0.0 0.0 21.35918812510335 0.0 108-109 1.858007361425166E-4 0.0 0.0 21.67216946513542 0.0 110-111 1.858007361425166E-4 0.0 0.0 21.98640496013645 0.0 112-113 1.858007361425166E-4 0.0 0.0 22.35029570187157 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTTCA 610 0.0 85.88978 1 GTACAAC 270 0.0 83.793144 1 GTATAAC 270 0.0 83.793144 1 CGCATAG 775 0.0 83.73624 1 GCAAGTA 50 9.404175E-10 83.24758 2 GTGTACT 590 0.0 79.60825 4 AGTGTAC 595 0.0 77.94729 3 TACAACG 285 0.0 77.197 2 ATAACGC 285 0.0 77.19341 3 AGATTAC 985 0.0 75.4601 2 ATAGTAC 870 0.0 74.48868 4 GAGTTCT 570 0.0 74.160416 1 GCATAGT 880 0.0 73.65248 2 CATAGTA 885 0.0 73.23296 3 CTTAGGA 140 0.0 72.20454 2 ATAAACG 280 0.0 72.20118 3 TAGTACT 915 0.0 70.82531 5 GACCGTT 145 0.0 69.705 7 AGTTCTT 620 0.0 69.05329 2 TAGGACC 150 0.0 67.38151 4 >>END_MODULE