FastQCFastQC Report
Fri 10 Feb 2017
SRR3556786.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556786.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5698394
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1263312.2169579709651526No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT552300.9692204505339576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT529290.9288406522960679No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190680.33462059661020277No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA173330.30417342149384546No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT138600.2432264248488258No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96770.16981977729163691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC94830.1664153092959174No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA93370.16385318389707698No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT93090.16336181738223085No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC91120.15990470297420642No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC90550.15890442114041256No Hit
TATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAC71920.1262109990990444No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT67490.11843687888201482No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67030.11762963389333908No Hit
GGTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAA63280.11104883235522149No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT63090.11071540507729019No Hit
GTACATGGGACTGCTGCTAGCTGGAGCTCCTCGCCCTTGCTCACTTTTTC62990.11053991703627374No Hit
ACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAA61120.10725829066926577No Hit
ACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAACACAGG57590.10106356282138441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA712950.069.8233951
GGTATCA298250.062.1891941
GACCGTT19500.052.4546287
TATCAAC964100.051.672592
ATCAACG1013650.049.064133
TCAACGC1043300.047.749554
AACGCAG1045700.047.6854556
CAACGCA1064700.046.761885
GGACCGT28150.046.054086
TAGGACC24100.045.4036524
TTAGGAC24700.043.5784343
CGCAGAG1211950.041.0656668
ACGCAGA1212800.041.0515947
GCAGAGT1258600.039.538849
GTACTGG61950.036.224131
TACTGGT58950.036.1154482
GGTTCAC59100.034.614736
AGGACCG33250.033.9822355
ATGGGAC121000.033.6662865
ACGGTAC19550.033.1565783
Produced by FastQC (version 0.11.5)