FastQCFastQC Report
Fri 10 Feb 2017
SRR3556771.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556771.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12133056
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1595411.3149284071548009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT724300.5969641943464202No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT483970.3988854910090252No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194350.16018223273674825No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA193130.15917671524799687No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT171040.14097025514429343No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA135220.11144760231882223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA312150.084.338751
GTATCAA831750.075.2571
TATCAAC1072200.058.3273122
ATCAACG1119250.055.7950133
TCAACGC1147100.054.54164
CAACGCA1165250.053.574685
AACGCAG1183700.052.910436
ACGCAGA1381200.045.2973257
CGCAGAG1398950.044.7310948
GCAGAGT1479300.042.285399
GGACCGT35250.033.065046
TACTGGT92850.032.8573572
GTACTGG111100.031.0803341
AGAGTAC1443150.030.941610-11
CAGAGTA1462750.030.3603210-11
GTACATG1040000.030.317011
TACATGG1045600.030.0591432
ACATGGG1051700.029.7996083
GAGTACT821500.029.77666312-13
GGTTCAC96250.029.6560046
Produced by FastQC (version 0.11.5)