FastQCFastQC Report
Fri 10 Feb 2017
SRR3556765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2897978
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT629642.172687301283861No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292371.0088758437779721No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251560.8680535186947589No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132960.4588026548165652No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA88040.3037980274522443No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58720.20262403648336874No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58150.20065714784584288No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA57720.19917335466314792No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC52410.18085023419777516No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT50770.17519111601261292No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA49960.17239606373823405No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA49230.1698770660094728No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47440.1637003455512775No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC47190.16283767509622227No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT41020.14154696826545957No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA38070.13136745689580803No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA37960.13098788189558375No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA32100.11076688642908952No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC31380.10828239551853051No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29600.10214018187853738No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG29420.10151905915089764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG9050.057.2283971
TACAACG6450.048.86522
AGATTAC12200.045.3321882
ATAGTAC11550.044.274644
ACAACGC7600.043.032363
AACGCAG556600.039.9546176
GCATAGT13800.038.3525662
ATAACGC7100.037.6877483
AGTGTAC12700.037.4569443
CATGGGT39050.037.001884
GTATCAA587750.036.9591641
TAGTACT14300.036.17615
CGCAGAG626650.035.4788068
ACGCAGA631200.035.1853757
TAACGCA7800.035.0672534
ACATGGG510950.034.726943
GTACATG527800.034.6831
TACATGG527900.034.61742
CATGGGC133800.034.219464
GCAGAGT653500.033.9483159
Produced by FastQC (version 0.11.5)