FastQCFastQC Report
Fri 10 Feb 2017
SRR3556699.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556699.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5439545
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT931561.7125697094150338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458790.8434345152030179No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT335720.6171839740272393No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197560.36319214198981714No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA127750.23485420195990658No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA114320.21016463693194926No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT103720.19067771293371044No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97450.17915101354984653No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC84670.1556564014085737No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC83760.15398346736721547No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80390.14778809624702066No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA79740.1465931433603362No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA79180.14556364548873113No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA72560.13339350993511406No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA71270.13102198805230952No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG61710.11344698867276583No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT59200.10883263214110739No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59040.10853848989207737No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA57200.10515585402823215No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG15150.059.3206561
TACAACG10400.051.4511952
ATAGTAC19550.047.4408154
AGATTAC25350.043.6235732
GCATAGT21700.042.467652
ACAACGC13750.039.779513
TAGTACT23600.039.551415
AGTGTAC24150.038.6506543
TATCACG11700.038.6201552
AACGCAG897700.037.6441236
GATTACT30350.036.435823
GACCGTT9800.035.1865127
CATGGGT68650.034.2948534
TCACGCA13600.034.0980874
CGCAGAG1006550.033.7044078
GTACAAC18050.033.6329231
GTGTACT29400.033.5687454
ACGCAGA1008700.033.5252387
GTATCAA969850.033.4574551
TAACGCA12500.033.293724
Produced by FastQC (version 0.11.5)