FastQCFastQC Report
Fri 10 Feb 2017
SRR3556697.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556697.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132225
Sequences flagged as poor quality0
Sequence length125
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT679913.188734772362204No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346491.6250161216569545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT269981.2661890747927635No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156810.7354289533234063No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA124340.5831467129407074No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA113140.530619423372299No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT112740.5287434487448557No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA75050.3519797394740236No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74420.34902507943580063No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG72330.3392231120074101No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA65680.30803503382616754No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61410.2880090046782117No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA60390.28322526937823167No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA59810.2805051061684391No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC57100.26779537806751164No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC54110.253772467727374No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA52430.2458933742921127No Hit
GGTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51070.23951506055880592No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG49330.23135457092942818No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC48880.22924409947355462No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG47450.22253749018044533No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45730.2144707992824397No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44120.20692000140698097No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT43280.20298045468935033No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT42550.19955680099426656No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA42120.19754012826976516No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC39660.18600288431098969No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA39380.18468970207177948No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33440.1568314788542485No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA33280.15608108900327125No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA32490.15237603911407097No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32180.15092215877780252No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT31490.14768610254546308No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31410.14731090761997445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC31360.14707641079154404No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA31030.14552873172390343No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT30380.1424802729543083No Hit
GTTTCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30290.14205817866313358No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC29760.1395725122817714No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT29160.13675855034060663No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA28920.13563296556414073No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA28880.13544536810139643No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC28430.13333489664552287No Hit
GTACATGGGACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA27750.1301457397788695No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26790.12564340067300592No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAACACAGGTGGAAAA26380.12372052667987665No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC26210.1229232374632133No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT24550.11513794275932418No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA24030.11269917574364807No Hit
CGCATAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23930.11223018208678728No Hit
GGTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAA23930.11223018208678728No Hit
CACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCC23250.1090410252201339No Hit
GAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAA23210.10885342775738958No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA22960.10768094361523761No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT22830.10707125186131858No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA22710.10650845947308564No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT22590.10594566708485267No Hit
GTACAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22360.10486698167407285No Hit
GTATAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22260.10439798801721208No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG22240.10430418928583991No Hit
TATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAC22100.1036475981662348No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA22090.10360069880054873No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG22020.10327240324074617No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21790.10219371782996635No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC21430.10050534066526749No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC6000.089.306691
ATAACGC6200.087.265223
CGCATAG14600.085.228761
GTACAAC5400.081.588831
CATAGTA15900.078.152093
ATAGTAC15950.077.9071044
CGCAAGT2000.077.399131
GCATAGT16100.077.1830752
TACAACG5750.076.518332
TAGTACT16550.076.160425
GCAAGTA2050.075.408642
AGTGTAC12900.075.125853
AGATTAC18900.072.354852
GTGTACT13850.071.689934
CTGAGTA4200.069.364664
GGTTTCA13100.067.264081
ATAAACG5700.066.7569663
GAGTTCT11650.065.925971
GCTGAGT4450.064.131693
TAGGACC2600.064.0289154
Produced by FastQC (version 0.11.5)