FastQCFastQC Report
Fri 10 Feb 2017
SRR3556692.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556692.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10600
Sequences flagged as poor quality0
Sequence length125
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1881.7735849056603774No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT910.8584905660377359No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.820754716981132No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA340.32075471698113206No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.25471698113207547No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.25471698113207547No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT270.25471698113207547No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.21698113207547168No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT220.20754716981132076No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180.16981132075471697No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA170.16037735849056603No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA170.16037735849056603No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT150.14150943396226415No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA150.14150943396226415No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA150.14150943396226415No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG140.1320754716981132No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA140.1320754716981132No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG140.1320754716981132No Hit
GAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAA130.12264150943396226No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG130.12264150943396226No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC120.11320754716981132No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.11320754716981132No Hit
GTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC110.10377358490566038No Hit
GTATCAACGCAGAGTACATGGGCCATCTCATCCCTGCACATCTCCGAGCC110.10377358490566038No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA110.10377358490566038No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTTCT150.00411524959.73222772-73
AACGCAG2800.038.2181636
CGCAGAG3000.035.670288
ACGCAGA3100.034.5196277
GTATCAA2800.033.97171
AGTACTT1850.032.1353912-13
GCAGAGT3150.032.084389
TACATGG2450.031.5451492
TCAACGC3400.031.4737784
CAACGCA3400.031.4737785
ACATGGG2500.030.9142463
GTACATG2409.094947E-1229.7252371
ATCAACG3300.028.8244723
AGAGTAC3200.028.79632410-11
TATCAAC3400.027.976692
TGGGAAG1305.8448026E-527.4386776
TACTTTT2350.026.56297514-15
CAGAGTA3150.026.4224329
ATGGGAA1357.5582095E-526.422435
CATGGGA1607.774677E-626.0095834
Produced by FastQC (version 0.11.5)