FastQCFastQC Report
Fri 10 Feb 2017
SRR3556687.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556687.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1722954
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284871.65338134390123No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136910.7946236521694717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108490.6296743848065589No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53290.3092943862691633No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35400.2054610860185472No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA30650.1778921549849851No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29750.17266856805231015No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC25330.1470149522273955No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT24310.1410948870370306No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.1408046877629931No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT23250.13494266242743566No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA22790.1322728291062907No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC22190.12879043781784075No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA18140.10528429662080357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5100.064.1786351
ATAGTAC7100.046.889294
GCATAGT8000.041.6154562
TAGTACT8700.039.6326185
AACGCAG287400.037.770966
TCACGCA4300.037.3283924
GTATCAA281100.036.858351
AAACGCA6800.036.7184525
GGACCGT5100.036.1356246
TACAACG3650.035.8332672
ATAACGC4550.035.2773863
TATCACG4600.034.8949472
CATGGGG67400.034.2228244
TAACGCA4700.034.151514
GTATCAC6150.033.8681031
ACATGGG258700.033.7804073
CGCAGAG324600.033.5338828
GACCGTT3550.033.492357
ACGCAGA324700.033.3953937
ACGGTAC4100.033.3493963
Produced by FastQC (version 0.11.5)