FastQCFastQC Report
Fri 10 Feb 2017
SRR3556686.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556686.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3599901
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT537271.492457709253671No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261650.7268255432579951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199160.5532374362517192No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA111030.3084251483582465No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT94670.2629794541572115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90380.2510624597731993No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA66680.1852273159734115No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA64530.17925492951056154No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC55980.1555042763676001No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53610.14892076198762133No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC53280.1480040701119281No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50560.1404483067728807No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT49010.13614263281129121No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46290.12858686947224382No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT45800.1272257209295478No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA41200.11444759175321766No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37200.10333617507814798No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36800.10222503341064103No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA36260.10072499215950662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6150.041.610471
GGACCGT12500.033.294226
GACCGTT8450.032.3654757
GTATCAA660900.031.2916431
AACGCAG679650.030.7657766
TTAGGAC10950.030.405683
TACAACG5700.030.2485082
CATGGGG117400.029.1192764
CGCAGAG743200.028.0790398
ACGCAGA747100.027.9245037
TATCAAC743750.027.7305282
CATGGGC109750.027.627814
AGTACTT342650.027.61811412-13
ATCAACG745950.027.55313
TCAACGC757800.027.4046844
CAACGCA762550.027.2417765
GCAGAGT772450.027.0311769
ACATGGG546350.026.82423
CATGGGT35300.026.7795474
ATAGTAC10100.026.489394
Produced by FastQC (version 0.11.5)