FastQCFastQC Report
Fri 10 Feb 2017
SRR3556682.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556682.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4363333
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT415040.9511994615125638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201900.46271966865696473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157260.3604125561812495No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72350.1658136108337365No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70630.16187167012006648No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT57020.1306799183101542No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC56060.1284797653536872No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT50000.11459129981598928No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC47760.10945760958423296No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA420750.036.6324541
AACGCAG454250.034.645446
TATCAAC491150.031.1723882
ACGCAGA503700.031.1025337
ATCAACG493150.030.9842623
CGCAGAG508500.030.9375538
TCAACGC501750.030.7849794
CAACGCA506700.030.554645
GGACCGT13750.029.403116
CATGGGG123800.029.2951874
TAACGCA6700.029.283724
GCAGAGT539700.029.204149
ACATGGG406950.029.000573
CGCATAG7950.028.445061
GACCGTT9900.028.2260277
ACGGTAC10350.028.1476943
GTACATG442650.027.963611
TACATGG442600.027.9555262
CATGGGC95000.025.8472024
AGTACTT285250.025.461412-13
Produced by FastQC (version 0.11.5)