FastQCFastQC Report
Fri 10 Feb 2017
SRR3556681.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556681.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1695906
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265941.5681293656605966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95870.5653025580427218No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89560.5280953071691473No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36590.21575488264090106No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC35260.20791246684662948No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT33990.20042384424608436No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC30640.18067039092968595No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29040.17123590576364492No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA27870.1643369384859774No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26330.1552562465136629No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT20440.12052554799617432No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20270.11952313394728245No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT18600.10967589005522711No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18390.10843761387718423No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA18260.10767106195744339No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG242300.042.034546
GTATCAA239900.041.935721
GACCGTT3500.039.0716827
TATCAAC265300.037.8525122
ATCAACG268000.037.3602373
ACGCAGA275200.036.8364947
TCAACGC274400.036.813884
CGCAGAG275400.036.8097428
CAACGCA277000.036.4254045
TACTGGT13400.036.384082
GCAGAGT288550.035.070419
GTACTGG16050.034.864261
GGACCGT6300.033.975386
GGTTCAC14400.033.8574076
TAACGCA2650.033.6548584
TACAACG2650.033.6548582
ACATGGG227800.033.5652283
CATGGGT25350.033.5397954
CATGGGG61450.032.8004724
TACATGG241700.032.7664872