FastQCFastQC Report
Fri 10 Feb 2017
SRR3556680.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556680.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4363798
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT711171.6297042163729851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346110.7931393707958068No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262150.6007381643238299No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114460.26229445084305003No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC107710.2468262738101076No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC94730.21708154227120502No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT93090.21332334814764567No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA69250.15869203844907578No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA67150.15387971670549372No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT64190.14709663462882563No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62840.14400299922223714No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT54280.12438705916268351No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA48990.11226459153242199No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA46500.10655855289360323No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA45810.10497736146356912No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT43640.10000462899520098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA554100.046.511391
AACGCAG604700.042.9058386
TATCAAC661000.038.883952
ATCAACG671050.038.2825623
TCAACGC686000.037.6389354
CGCAGAG691950.037.4355748
ACGCAGA692750.037.400927
CAACGCA696000.037.192115
GCAGAGT726600.035.642179
TACTGGT34950.034.70322
GACCGTT10250.033.6415337
ACATGGG522600.032.9117243
GTACTGG41850.032.853081
TACATGG549650.032.223362
GTACATG551200.032.1893581
CATGGGT43100.030.3470924
CATGGGG165400.030.1576924
GGTTCAC40150.029.467226
GGACCGT17350.029.1266866
CATGGGA259550.029.0448994