FastQCFastQC Report
Fri 10 Feb 2017
SRR3556679.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556679.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1678798
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295151.7581031190172969No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144140.8585904915302496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111930.6667270273135899No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55960.3333337304428526No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40600.2418396972119338No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA29990.17863971722625355No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA27200.16202068384641868No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC27170.16184198456276455No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26690.15898279602429835No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT25960.15463444678871432No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC24880.14820127257716534No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23720.14129156694253864No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA21640.12890174994251838No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA21400.1274721556732853No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18070.10763653518767594No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA17300.10304992024055305No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4700.064.602181
TACAACG4150.057.3082582
TAACGCA4500.056.813084
ATAACGC5350.050.009343
ACAACGC5300.045.993913
GTATAAC5750.045.5574761
GTACAAC5950.045.026731
TATCACG4150.040.115782
TCACGCA4200.039.637034
AACGCAG289000.038.5535286
ATAGTAC8050.036.928914
GTATCAA294900.036.540851
AAACGCA5700.035.4647145
CATGGGG61700.035.4613344
AGATTAC8750.034.6551252
ACGCAGA327400.033.9771927
CGCAGAG328800.033.9229358
ACATGGG259800.033.366513
GTACTGG12950.033.1007271
TACATGG270850.032.928182