FastQCFastQC Report
Fri 10 Feb 2017
SRR3556675.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556675.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3805501
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325160.8544472856530585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163640.4300090842178204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126520.3324660800246801No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57280.15051894612562183No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46790.12295358745142886No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC40190.10561027312829506No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT38450.10103794480674161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA309600.039.522281
AACGCAG331600.037.469496
ATCAACG361500.033.630323
TATCAAC362950.033.5955662
TCAACGC365950.033.465054
ACGCAGA372800.033.2009747
CAACGCA369500.033.175715
CGCAGAG376350.032.919398
CGCATAG5900.032.2788351
ACATGGG309650.031.3517023
TAACGCA5950.030.9735344
GCAGAGT400850.030.892529
GTACATG331100.030.35921
TACATGG333000.030.2256662
CATGGGG104950.029.398894
TACAACG6700.029.2821032
ACGGTAC6800.027.9760933
GTACTGG29100.027.6096321
AGAGTAC390000.025.86938510-11
AGTACTT226000.025.86099812-13
Produced by FastQC (version 0.11.5)