FastQCFastQC Report
Fri 10 Feb 2017
SRR3556674.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556674.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3019172
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306161.0140528595257243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151540.5019256935345188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114860.3804354306412487No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56460.1870049139300444No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43070.14265500607451315No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA35250.11675386496695121No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33050.10946709892646063No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6350.054.3611341
GTATCAA312400.036.9022451
AACGCAG325000.036.6065646
ACGCAGA365600.032.4926037
CGCAGAG366650.032.415778
TATCAAC361100.031.7146572
ATCAACG363000.031.480533
TCAACGC367950.031.4609894
CAACGCA373950.031.1787935
ACATGGG297600.030.9466573
GCAGAGT387650.030.7670849
GTACATG314800.030.5520861
TAACGCA5450.030.5461714
CATGGGG85650.030.1965374
TACATGG318600.030.1036952
ATAGTAC11150.029.8612794
TAGTACT11950.029.3548185
ACAACGC6850.026.9070663
GCATAGT13400.026.1805292
AGTACTT219450.025.57700212-13
Produced by FastQC (version 0.11.5)