FastQCFastQC Report
Fri 10 Feb 2017
SRR3556671.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556671.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6217754
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT724661.165469074524338No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT368620.5928507303441082No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT272310.43795557045196704No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130670.21015627186279803No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88940.14304200519994842No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT76490.12301869774841527No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA71560.1150897896571656No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65860.10592249226971669No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG717550.038.0047156
GTATCAA716100.037.243581
CGCATAG10900.036.5846141
ACGCAGA800800.034.009267
CGCAGAG804550.033.9394158
GCAGAGT851450.032.0699429
TATCAAC832250.031.9171282
ATCAACG832400.031.8325543
TCAACGC846300.031.724194
CAACGCA854650.031.5603225
TATCACG9400.030.3583262
CATGGGG184550.029.6692474
CATGGGT75800.028.8628714
ACATGGG630250.028.5347083
TAACGCA9250.028.2795094
GTACATG676350.027.957341
TACATGG677900.027.7569982
AGAGTAC833550.026.18381310-11
AGTACTT461750.025.70771612-13
GAGTACT416600.024.13378512-13
Produced by FastQC (version 0.11.5)