FastQCFastQC Report
Fri 10 Feb 2017
SRR3556668.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556668.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519422
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370801.4717661431868103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT182260.7234198955157175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142850.5669951282476695No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA66140.2625205305026312No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65930.26168700598788136No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC56860.2256866852794014No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53220.21123892702373798No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC50830.20175262421301393No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50590.20080002476758557No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT45850.18198618572037556No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA34000.13495158810235044No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA33090.1313396485384346No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT32440.12875969170706614No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.12510806049959078No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG30030.11919400560922308No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT29180.1158202159066643No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC27840.11050153566968932No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA26790.10633391309594026No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA25540.10137245765100092No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG6750.055.557581
TACAACG3900.045.7319562
AGATTAC10600.045.4299552
ATAGTAC8500.043.3629344
TAACGCA6600.040.5335354
ATAACGC6900.040.494373
GACCGTT5350.038.891997
ACGGTAC8600.038.019833
GCATAGT10200.037.8857882
GTATAAC6600.036.9782521
GATTACT13600.034.9701083
TACGCAG5300.034.7721675
TTCAACG14700.034.375384
AACGCAG427450.033.75446
TAGTACT10950.033.6607255
TACTGGT21300.033.214712
GTACTGG22950.032.94031
GTATCAA428000.032.7114681
CATGGGG91100.031.8454444
GGTTTCA15600.031.6708681
Produced by FastQC (version 0.11.5)