FastQCFastQC Report
Fri 10 Feb 2017
SRR3556667.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556667.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3651362
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT453791.2427965235985914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228910.6269167505166565No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174640.47828728019845745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86430.23670619346972446No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA61490.16840291376204278No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA57320.15698251775638788No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT48860.13381308125570676No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40630.11127354669298745No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC39810.10902780934894979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG8950.047.878411
TACAACG6550.047.1997952
ACAACGC8200.041.3269233
TCACGCA6750.039.6351854
TAACGCA7800.038.8729674
AACGCAG467800.037.2882466
TATCACG6950.036.7842332
GTATCAA476200.035.4443551
AGATTAC16050.034.079312
TAGTACT13600.033.223615
ACGCAGA524450.033.1470877
CGCAGAG525950.033.075168
GCAGAGT556400.031.222329
ATAGTAC14100.031.2021644
ATCACGC8400.031.1419263
TATCAAC544150.030.8767492
ATCAACG543150.030.834663
TCAACGC556200.030.5494484
CATGGGG109400.030.3241734
CAACGCA563450.030.282985