FastQCFastQC Report
Fri 10 Feb 2017
SRR3556652.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556652.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2197427
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276601.2587448866333217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135150.6150374961261511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104390.4750555991166032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52540.2390978175839288No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52510.23896129427735258No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT37850.17224690513040936No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30160.13725143087802233No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG25580.11640887274070993No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24120.10976473848733086No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC23160.10539599267688983No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA23040.10484989945058472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5500.048.705481
TAACGCA4650.042.195384
ATAGTAC7700.037.0641864
TACAACG4000.034.1886332
CATGGGT36400.033.812164
AACGCAG315700.033.07156
AGATTAC8900.032.0674972
TAGTACT8600.031.802655
GTATCAA320000.031.494521
CATGGGC63300.030.5269624
ACGGTAC4300.030.4199283
ACAACGC4500.030.38923
GCATAGT9900.030.029542
ACATGGG287850.029.8679923
CGCAGAG351250.029.622778
ACGCAGA350750.029.597197
CATGGGG67950.028.9629274
GTACATG317000.028.4123841
GCAGAGT366800.028.3993729
AAACGCA6700.028.3974365