FastQCFastQC Report
Fri 10 Feb 2017
SRR3556639.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556639.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028703
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130791.2714068103232905No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57230.556331613692193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54680.5315431178872814No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA25920.2519677691228664No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21900.21288943455982923No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18200.1769218131958398No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT18060.17556087617125643No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC16890.16418733103723815No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC15780.15339704462804135No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA14790.1437732756684874No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT13260.12890017818554042No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA12660.12306759093732593No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC12390.1204429266756294No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11690.11363824155271249No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA11230.10916659132908138No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10630.10333400408086688No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT10630.10333400408086688No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.10148701811893228No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT3650.048.8527346
GACCGTT2500.045.1724977
GTACTGG9850.038.649341
TACTGGT9000.038.304162
GGTTCAC8750.036.6814236
ACTGGTT9850.034.998733
CGCATAG2400.034.6988561
TAACGCA1607.050403E-933.4335944
AACGCAG153600.033.0853236
TTAGGAC4150.032.9412653
CATGGGG35600.032.3900954
GTATCAA156050.032.0956881
ACATGGG139400.031.466913
AGGACCG6100.031.1803235
CGCTAAC1450.030.76596362-63
CATGGGC32500.030.5417294
TAGGACC5700.030.2401284
TGGTTCA10850.030.1296045
TCACTAT10700.029.9964949
CTGGTTC11500.029.9771694
Produced by FastQC (version 0.11.5)