FastQCFastQC Report
Fri 10 Feb 2017
SRR3556633.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556633.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3029875
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400791.322793844630554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205840.6793679607244524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144960.4784355790255374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77560.2559841577622839No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA52020.17169025124798876No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA41460.13683732827261852No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT39900.13168860101489335No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37910.12512067329510293No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37510.12380048681876316No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG5150.042.7476121
GTATCAA365850.038.2355161
AACGCAG373450.037.81766
ACGCAGA422300.033.443017
CGCAGAG425600.033.2395678
TATCAAC423550.032.923842
ATCAACG429800.032.472733
TCAACGC435800.032.338834
CAACGCA439050.032.004685
CATGGGT33250.031.9993674
ACATGGG354650.031.9790523
GCAGAGT446700.031.682799
GTACATG368500.031.4696371
CATGGGG97500.031.4575654
TACATGG370100.031.2222582
GGACCGT7950.029.9069986
CATGGGC87000.029.3784714
AGTACTT241800.026.61041312-13
GACCGTT5150.026.5460937
GTACTGG22400.026.29691
Produced by FastQC (version 0.11.5)