FastQCFastQC Report
Fri 10 Feb 2017
SRR3556627.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556627.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3446853
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT291950.8470044994666149No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148200.4299574133274613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111170.32252608393801535No Hit
GTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCAT110310.32003105441398283No Hit
GTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGGGAAGATAG96860.2810099531369629No Hit
GTCCTGCCCAGTTCCTGTTTCTGTTAGTGCTCTGGATTCGGGAAACCAAC89210.2588157951615575No Hit
CTCTTAGATAGTGATGGAAAGACATATTTGAATTGGTTGTTACAGAGGCC85010.2466307672534918No Hit
GATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCACCCTCACGTT74610.21645831719542435No Hit
CTCCTATGTTCATTTCCTCAAAATGATGAGTCCTGCCCAGTTCCTGTTTC71370.20705843852348796No Hit
CCTATGTTCATTTCCTCAAAATGATGAGTCCTGCCCAGTTCCTGTTTCTG70440.20436032520098768No Hit
GGTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGC70010.20311281043897145No Hit
GTGCTGGGACCAAGCTGGAGCTGAAACGGGCTGATGCTGCACCAACTGTA68800.1996023619226001No Hit
GGCCAGGCCAGTCTCCAAAGCGCCTAATCTATCTGGTGTCTAAACTGGAC67240.19507649441389002No Hit
GTCTGGGACTGTGCTGGCAAGGCCTGGGGCTTCAGTGAAGATGTCCTGCA66410.19266850080348652No Hit
ACATTGTACAGTGTGGGTTTACCAGTGGACTTGTCCACGGTCCTCTCGGT59090.1714317378780006No Hit
GTCCAACTGTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCA58040.16838548090098418No Hit
ATCTAAGAGGCTCTGACTTGACTTGCAAGAGATGGAGGCTGGTTGTCCAA57850.16783425344800026No Hit
CCCTTAGCACTGAAGGTGCCATTGGGATGGCTTTCCATGATTTTAATTTT57630.1671959900813873No Hit
CTTAGATAGTGATGGAAAGACATATTTGAATTGGTTGTTACAGAGGCCAG54250.15738994381251534No Hit
TGCATACACAGAGCAACTGGACACCCAGGGCCTGCCTGGTTGAGCGCTAG54100.15695476424437013No Hit
CCATTGGACAACCAGCCTCCATCTCTTGCAAGTCAAGTCAGAGCCTCTTA52850.15332826784316012No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51150.14839623273751448No Hit
CTCTAACACTCATTCCTGTTGAAGCTCTTGACAATGGGTGAAGTTGATGT51080.1481931489390467No Hit
GGGTAAAACAGAGGCCTGGACAGGGTCTGGAATGGATAGGGGCTATTTAT48520.14076608430936857No Hit
TCCTTGGTCAACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCT48290.14009880897154595No Hit
CTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCTGCTCAT48030.13934449772009422No Hit
GTTAACATCTGGAGGTGCCTCAGTCGTGTGCTTCTTGAACAACTTCTACC47880.13890931815194904No Hit
GTCCCAGCACCGAACGTGAGCGGAAAATGTGTACCTTGCCAGCAATAATA46410.13464455838412603No Hit
GTATAGCTGTTATGTCGTTCATACTCGTCCTTGGTCAACGTGAGGGTGCT46200.13403530698872276No Hit
CCGCAACAGTGGTAGGTCGCTTGTGGGGAAGCCTCCAAGACCTTAGAAGG46100.13374518727662596No Hit
ACGTGAGGGTGCTGCTCATGCTGTAGGTGCTGTCTTTGCTGTCCTGATCA46090.13371617530541627No Hit
GAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAACACTCA45640.13241063660098068No Hit
TCACTCTCCTATGTTCATTTCCTCAAAATGATGAGTCCTGCCCAGTTCCT43860.12724650572565757No Hit
GGTTGTTACAGAGGCCAGGCCAGTCTCCAAAGCGCCTAATCTATCTGGTG43630.12657923038783492No Hit
GTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTT43050.12489653605767348No Hit
GTGCTAAGGGTGTGGCTAGTGTTTGTGTGGAAGACTGGAATAACAGGAAG42940.124577404374367No Hit
GGTATAGGTCTCTCCGGAGTTCCATTCCTCCTCTGTCACAGTCAGGATGC42930.12454839240315732No Hit
GTCTATTACTGTACATACGGTAGTAGCTACTATGCTATGGACTACTGGGG42520.12335890158356043No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA41240.11964536926872135No Hit
GTGCAGGGCCAGAGAAGCCATCCCGTGGTGGGACGAACACATTTACATTG41210.1195583333550923No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT41150.11938426152783423No Hit
GTAATAGACCGCAGAGTCCTCATTTGTCAGGCTGCTGAGCTCCATGTAGG40680.11802069888097926No Hit
CCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCA40020.11610590878114037No Hit
GTGATGGAAAGACATATTTGAATTGGTTGTTACAGAGGCCAGGCCAGTCT39570.11480037007670475No Hit
GTGCTGGCAAGGCCTGGGGCTTCAGTGAAGATGTCCTGCAAGACTTCTGG39250.11387198699799499No Hit
GATGTTAACTGCTCACTGGATGGTGGGAAGATGGATACAGTTGGTGCAGC39220.11378495108436595No Hit
CAGTTGGACTGATCAGGACAGCAAAGACAGCACCTACAGCATGAGCAGCA37740.10949117934533326No Hit
GGATGGAGCTGGGGAGCTGGTGGTGGCGTCTCAGGACCTTTGTCTCTAAC37640.10920105963323645No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC37630.10917204766202677No Hit
TTGCTGTCCTGATCAGTCCAACTGTTCAGGACGCCATTTTGTCGTTCACT37060.10751836530307501No Hit
TCTTCCCACCATCCAGTGAGCAGTTAACATCTGGAGGTGCCTCAGTCGTG36950.10719923361976853No Hit
CTCCAAGACCTTAGAAGGGAAGATAGGATGGAGCTGGGGAGCTGGTGGTG35960.10432704847001019No Hit
ACATAACAGCTATACCTGTGAGGCCACTCACAAGACATCAACTTCACCCA35340.10252830625501No Hit
GTTCAGGACGCCATTTTGTCGTTCACTGCCATCAATCTTCCACTTGACAT35160.10200609077323575No Hit
TGCTGTAGGTGCTGTCTTTGCTGTCCTGATCAGTCCAACTGTTCAGGACG34550.10023636052944526No Hit
ATGCTGCACCAACTGTATCCATCTTCCCACCATCCAGTGAGCAGTTAACA34480.1000332767309775No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3250.049.422751
AACGCGG1004.1909516E-947.548786
GTATCAA272800.039.8419531
TACAACG2650.038.128742
AACGCAG295400.037.7661866
TACTGGT15400.036.6650472
GTACTGG17600.036.5054321
ACAACGC2800.036.086133
TAACGCA3050.035.076974
ACGGTAC5850.034.5439833
CATGGGT19300.033.8754234
ATCAACG322700.033.760813
TCAACGC329250.033.4682734
ACGCAGA332500.033.46297
TATCAAC325350.033.4492872
ACTGGTT16250.033.284143
CAACGCA332600.033.1311765
TCACGCA3600.033.0199854
ATAGTAC5100.032.6315164
CATGGGG81950.032.5646744
Produced by FastQC (version 0.11.5)