##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3556626.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 888930 Sequences flagged as poor quality 0 Sequence length 125 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.92228971910049 34.0 33.0 34.0 32.0 34.0 2 32.86930354471106 34.0 33.0 34.0 32.0 34.0 3 33.0381593601296 34.0 33.0 34.0 32.0 34.0 4 33.040232639240436 34.0 33.0 34.0 32.0 34.0 5 33.08325739934528 34.0 33.0 34.0 32.0 34.0 6 36.626389029507386 38.0 37.0 38.0 34.0 38.0 7 36.765854454231494 38.0 38.0 38.0 35.0 38.0 8 36.91419571844802 38.0 38.0 38.0 35.0 38.0 9 36.95427761466032 38.0 38.0 38.0 35.0 38.0 10-11 36.84853981753344 38.0 38.0 38.0 35.0 38.0 12-13 36.91620150068059 38.0 38.0 38.0 35.0 38.0 14-15 37.0162324367498 38.0 38.0 38.0 36.0 38.0 16-17 36.92300012374427 38.0 38.0 38.0 35.0 38.0 18-19 36.894943358869654 38.0 38.0 38.0 35.0 38.0 20-21 36.93532336629431 38.0 38.0 38.0 35.0 38.0 22-23 36.89299832382753 38.0 38.0 38.0 35.0 38.0 24-25 36.84867537376397 38.0 38.0 38.0 35.0 38.0 26-27 36.634585962899216 38.0 38.0 38.0 34.5 38.0 28-29 36.68054177494291 38.0 38.0 38.0 34.5 38.0 30-31 36.61532066641918 38.0 38.0 38.0 34.0 38.0 32-33 36.424792728336314 38.0 38.0 38.0 34.0 38.0 34-35 36.11092661964384 38.0 38.0 38.0 33.0 38.0 36-37 35.93284623086183 38.0 38.0 38.0 31.5 38.0 38-39 35.727795776945314 38.0 38.0 38.0 29.5 38.0 40-41 35.403407467404634 38.0 37.5 38.0 27.5 38.0 42-43 34.986127141619704 38.0 37.0 38.0 24.5 38.0 44-45 34.589078442622025 38.0 36.5 38.0 20.0 38.0 46-47 34.05068790568436 38.0 36.0 38.0 15.0 38.0 48-49 33.519658465795956 38.0 34.5 38.0 15.0 38.0 50-51 33.07649589956465 38.0 33.5 38.0 15.0 38.0 52-53 32.78117905796857 38.0 32.0 38.0 15.0 38.0 54-55 32.49709650928644 38.0 29.5 38.0 15.0 38.0 56-57 32.3166379804934 38.0 27.5 38.0 15.0 38.0 58-59 32.10599372279032 38.0 26.0 38.0 15.0 38.0 60-61 31.96157290225327 38.0 26.0 38.0 15.0 38.0 62-63 31.866062569606157 38.0 25.5 38.0 15.0 38.0 64-65 31.730631208306615 38.0 25.0 38.0 15.0 38.0 66-67 31.52958331927148 38.0 25.0 38.0 15.0 38.0 68-69 31.38991146659467 38.0 24.0 38.0 14.5 38.0 70-71 31.231286490499812 38.0 24.0 38.0 14.0 38.0 72-73 30.95117894547377 38.0 24.0 38.0 14.0 38.0 74-75 30.82023837647509 38.0 23.5 38.0 14.0 38.0 76-77 30.668277029687378 38.0 18.5 38.0 14.0 38.0 78-79 30.521382448561752 38.0 16.0 38.0 14.0 38.0 80-81 30.428912850280675 38.0 15.0 38.0 14.0 38.0 82-83 30.25908507981506 38.0 15.0 38.0 14.0 38.0 84-85 30.125802369140427 38.0 15.0 38.0 13.0 38.0 86-87 30.079843182252823 38.0 15.0 38.0 13.0 38.0 88-89 30.041766505799107 38.0 15.0 38.0 13.0 38.0 90-91 29.972891566265062 38.0 14.0 38.0 13.0 38.0 92-93 29.899167538501345 38.0 14.0 38.0 13.0 38.0 94-95 29.831117185830156 38.0 14.0 38.0 13.0 38.0 96-97 29.749322781321364 38.0 14.0 38.0 13.0 38.0 98-99 29.70421574252191 38.0 14.0 38.0 13.0 38.0 100-101 29.60371457820076 38.0 14.0 38.0 13.0 38.0 102-103 29.51905380626146 38.0 14.0 38.0 13.0 38.0 104-105 29.496566096318045 38.0 14.0 38.0 13.0 38.0 106-107 29.381627349735076 37.0 14.0 38.0 13.0 38.0 108-109 29.2961583026785 37.0 14.0 38.0 13.0 38.0 110-111 29.21191769880643 37.0 14.0 38.0 13.0 38.0 112-113 29.083895244844925 36.5 14.0 38.0 13.0 38.0 114-115 28.995494583375518 36.0 14.0 38.0 13.0 38.0 116-117 28.83502919239985 36.0 14.0 38.0 13.0 38.0 118-119 28.690080208790345 35.5 14.0 38.0 13.0 38.0 120-121 28.610878809355064 35.0 14.0 38.0 13.0 38.0 122-123 28.46447470554487 35.0 14.0 38.0 13.0 38.0 124-125 27.29431282553182 31.5 14.0 37.5 13.0 38.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04588660795518251 1101 2 0.01084745040959234 1101 3 0.025729365224250955 1101 4 -0.0052305719221266145 1101 5 -0.0838723339503602 1101 6 0.16865373273151363 1101 7 0.3547840832940139 1101 8 0.16192848159369078 1101 9 0.4200120796650353 1101 10-11 0.4357462719628842 1101 12-13 0.3467247727900471 1101 14-15 0.23194763940955454 1101 16-17 0.1936038287677846 1101 18-19 0.2221059353689654 1101 20-21 0.2020714011551945 1101 22-23 0.28473024656341295 1101 24-25 0.21862972372690592 1101 26-27 0.2827673408221685 1101 28-29 0.2964810521127532 1101 30-31 0.20365871694262694 1101 32-33 0.1812452129520068 1101 34-35 0.27206010084281473 1101 36-37 0.2001258247929414 1101 38-39 0.21143900402965699 1101 40-41 0.34344705646147844 1101 42-43 0.19087057876080138 1101 44-45 0.13495943639715335 1101 46-47 -0.0021746550563435108 1101 48-49 -0.08260353938297982 1101 50-51 0.09384425338102886 1101 52-53 0.0888446681518289 1101 54-55 0.19321000863890703 1101 56-57 0.0701144943162717 1101 58-59 0.1196797789775843 1101 60-61 0.1283869047763453 1101 62-63 0.09635531003370446 1101 64-65 0.264703599379672 1101 66-67 0.2954387251170765 1101 68-69 0.1862123123958881 1101 70-71 0.15675642433395254 1101 72-73 0.3245766852835672 1101 74-75 0.3778308264524419 1101 76-77 0.5100190510507119 1101 78-79 0.38100746125147467 1101 80-81 0.3490659425433691 1101 82-83 0.5521936343354952 1101 84-85 0.6736571463000622 1101 86-87 0.47186054933492727 1101 88-89 0.6879280421122509 1101 90-91 0.4620502079894955 1101 92-93 0.5455910580614649 1101 94-95 0.5916079580137747 1101 96-97 0.5621940623465385 1101 98-99 0.6914571901893716 1101 100-101 0.5381006395209162 1101 102-103 0.85465351569432 1101 104-105 0.7202818837229579 1101 106-107 0.4422799831295947 1101 108-109 0.4294725288465493 1101 110-111 0.46064656774907675 1101 112-113 0.48542220971639694 1101 114-115 0.3582487730993833 1101 116-117 0.40548855362794 1101 118-119 0.6703228082201349 1101 120-121 0.6964349170791913 1101 122-123 0.5579416717430377 1101 124-125 0.5697023983861342 1102 1 0.22153315967931775 1102 2 0.2705457262716635 1102 3 0.21215177901735416 1102 4 0.07989873842270612 1102 5 0.12086904535998855 1102 6 0.3044295948004745 1102 7 0.27288753156987866 1102 8 0.3386526195247228 1102 9 0.3230293210443449 1102 10-11 0.3721686857559803 1102 12-13 0.40329804865211116 1102 14-15 0.30576229458196735 1102 16-17 0.3019012425608878 1102 18-19 0.34764472847241024 1102 20-21 0.4418343321896856 1102 22-23 0.42320007414961935 1102 24-25 0.43899610303724756 1102 26-27 0.2504397546152717 1102 28-29 0.4166319141817141 1102 30-31 0.3038742341840006 1102 32-33 0.3277969370899356 1102 34-35 0.20201699739453716 1102 36-37 0.16995341099983108 1102 38-39 -0.08597478907379497 1102 40-41 0.02448153922009766 1102 42-43 -0.07044407641161143 1102 44-45 -0.10534228774078258 1102 46-47 -0.010795344711517885 1102 48-49 0.16470249509978174 1102 50-51 0.26464166717412496 1102 52-53 0.2596420819449392 1102 54-55 0.21476173277684296 1102 56-57 0.25384294259212936 1102 58-59 0.38476598587413946 1102 60-61 0.1440119047763524 1102 62-63 0.1302992755509429 1102 64-65 0.22860446144864 1102 66-67 0.43336975959983803 1102 68-69 0.5784536917062333 1102 70-71 0.6546012519201589 1102 72-73 0.6807189266628804 1102 74-75 0.6337575505903708 1102 76-77 0.5391138786369183 1102 78-79 0.7570850474583715 1102 80-81 0.5823633563364758 1102 82-83 0.6669565653699792 1102 84-85 0.721609732506959 1102 86-87 0.6911493424383757 1102 88-89 0.5898676972846637 1102 90-91 0.32088641488605063 1102 92-93 0.4189746787511197 1102 94-95 0.5775993373241199 1102 96-97 0.6074526830361933 1102 98-99 0.5104227074307488 1102 100-101 0.422260122279539 1102 102-103 0.5006664467288005 1102 104-105 0.5575663664815806 1102 106-107 0.4643705003709755 1102 108-109 0.6358302874672397 1102 110-111 0.7505172574042476 1102 112-113 0.7909178993715713 1102 114-115 0.6287229110304189 1102 116-117 0.4512859674210432 1102 118-119 0.4607322909787541 1102 120-121 0.5590426756998816 1102 122-123 0.49705805105338285 1102 124-125 0.5971808466619919 1103 1 -0.16546506985275045 1103 2 -0.1638145887723539 1103 3 -0.1693778556374852 1103 4 -0.2362332028043781 1103 5 -0.13175770028830414 1103 6 -0.1531246774875612 1103 7 0.09673838205634411 1103 8 0.13087703874505507 1103 9 -0.0032435130280674684 1103 10-11 0.14419094897657914 1103 12-13 0.051078345164121686 1103 14-15 -0.06921185162228483 1103 16-17 -0.0259776293035614 1103 18-19 0.010942473194099023 1103 20-21 0.038252443911176215 1103 22-23 0.045674675380674046 1103 24-25 -0.017652252247678746 1103 26-27 -0.014904990238790106 1103 28-29 -0.049733331698220695 1103 30-31 -0.07617966580937718 1103 32-33 -0.003534967735006944 1103 34-35 0.013593499810319543 1103 36-37 0.12715813996931757 1103 38-39 0.09859132218439726 1103 40-41 -0.04281382281578061 1103 42-43 -0.03478238470469108 1103 44-45 -0.08751351019097342 1103 46-47 -0.2784007454660369 1103 48-49 -0.07486601539066129 1103 50-51 -0.13488944838613293 1103 52-53 -0.23110019087258848 1103 54-55 -0.11192062542026093 1103 56-57 -0.2641337186693704 1103 58-59 -0.016428045828817517 1103 60-61 -0.2532047789882945 1103 62-63 -0.27464148823223056 1103 64-65 -0.15667811019740085 1103 66-67 -0.11685382402581368 1103 68-69 -0.12195355728443502 1103 70-71 -0.11756992916395959 1103 72-73 0.01371891838966377 1103 74-75 -0.08026223893904572 1103 76-77 -0.051837706511005166 1103 78-79 -0.0193675222020957 1103 80-81 0.014307892705524239 1103 82-83 -0.09704171380137794 1103 84-85 -0.12195618846729772 1103 86-87 -0.015312865517145724 1103 88-89 -0.010510181794032292 1103 90-91 0.002653077140333693 1103 92-93 0.020707296727159985 1103 94-95 -0.3136751100251445 1103 96-97 -0.06226611039818408 1103 98-99 -0.0241510565509806 1103 100-101 -0.043723521575117275 1103 102-103 -0.21973612789559738 1103 104-105 -0.20811964847639075 1103 106-107 -0.5229642421665872 1103 108-109 -0.2643688901752306 1103 110-111 -0.08601539697393434 1103 112-113 -0.06683451640710203 1103 114-115 -0.11559235635987974 1103 116-117 -0.10982620082305061 1103 118-119 -0.10615871769157437 1103 120-121 -0.17225055184661642 1103 122-123 0.016183654998052077 1103 124-125 -0.16414828663604197 1104 1 -0.3040348513238982 1104 2 -0.5057597783430765 1104 3 -0.49276340451991274 1104 4 -0.4739692981344348 1104 5 -0.416328817129866 1104 6 -0.4014466791375355 1104 7 -0.38812658321231197 1104 8 -0.30842803315242406 1104 9 -0.20010355181739925 1104 10-11 -0.1481556047469752 1104 12-13 -0.0493689537228974 1104 14-15 -0.2349797649135681 1104 16-17 -0.21543079679214827 1104 18-19 -0.28445259062088013 1104 20-21 -0.1503824326841965 1104 22-23 -0.04272584203352636 1104 24-25 -0.3191811509687028 1104 26-27 -0.4618466071079652 1104 28-29 -0.4404446423525741 1104 30-31 -0.4331670166051609 1104 32-33 -0.47793409288330935 1104 34-35 -0.4260659137980767 1104 36-37 -0.3844623437426762 1104 38-39 -0.6715480048089901 1104 40-41 -0.4602701931852806 1104 42-43 -0.32858563008987574 1104 44-45 -0.34335073556568574 1104 46-47 -0.10625426302917163 1104 48-49 0.15222100290123564 1104 50-51 0.15937314434095384 1104 52-53 0.13231216582191507 1104 54-55 0.20457680507939813 1104 56-57 0.3117878626233974 1104 58-59 0.1846736347632998 1104 60-61 0.1867278166002997 1104 62-63 0.12230991800652546 1104 64-65 0.015831917631185632 1104 66-67 0.1375911354528867 1104 68-69 0.3502914882068495 1104 70-71 0.3237846351086908 1104 72-73 0.10645144101816939 1104 74-75 0.21435385880069546 1104 76-77 0.19178163259777747 1104 78-79 0.2842694377719326 1104 80-81 0.10595563604666225 1104 82-83 0.5989004670024194 1104 84-85 0.007052642046087243 1104 86-87 0.17284634812036614 1104 88-89 0.3111923485488326 1104 90-91 0.110102285372772 1104 92-93 0.023707696668925138 1104 94-95 0.2702143487482225 1104 96-97 0.1591589708033858 1104 98-99 0.14737292238430655 1104 100-101 0.11841228459201858 1104 102-103 -0.06072120621891841 1104 104-105 -0.046610947651242185 1104 106-107 -0.0532292914397452 1104 108-109 -0.15260064555143416 1104 110-111 -0.19781920127356045 1104 112-113 -0.29168420356562663 1104 114-115 -0.1925748710397528 1104 116-117 -0.052833662498088074 1104 118-119 -0.10421130250349364 1104 120-121 -0.2919427472926337 1104 122-123 -0.49762353960290184 1104 124-125 -0.44042897881977083 1105 1 0.035110745886214545 1105 2 -0.03173013579730366 1105 3 -0.07940856581023326 1105 4 -0.13635988226695162 1105 5 -0.06059647188138939 1105 6 0.18320545686944456 1105 7 0.06959442812160432 1105 8 0.08823020573161955 1105 9 0.15867587276847672 1105 10-11 0.09881954782494518 1105 12-13 0.19326356589349558 1105 14-15 0.0960424669957618 1105 16-17 0.24554348394019598 1105 18-19 0.20326972847241365 1105 20-21 0.0914981252931284 1105 22-23 0.23593283277030963 1105 24-25 0.260289206485524 1105 26-27 0.18105182358078764 1105 28-29 0.21192501762999427 1105 30-31 0.002706130735731449 1105 32-33 -0.05478064911696379 1105 34-35 0.06440923877384819 1105 36-37 0.11828961789638726 1105 38-39 -0.07716875459103534 1105 40-41 -0.06538484009025325 1105 42-43 -0.1326854557219619 1105 44-45 -0.1321353911890526 1105 46-47 -0.19438155160806758 1105 48-49 0.013646460617017908 1105 50-51 -0.06239712592931568 1105 52-53 -0.17463378012403297 1105 54-55 -0.13772964653350783 1105 56-57 -0.06006654016648838 1105 58-59 0.023106503115521093 1105 60-61 -0.14334585384434106 1105 62-63 -0.15206279341457574 1105 64-65 -0.04992571096515519 1105 66-67 -0.031893171434646206 1105 68-69 -0.022119584155834815 1105 70-71 0.10053659674774806 1105 72-73 -0.13152676299229427 1105 74-75 -0.19643210458204052 1105 76-77 -0.13011025929411701 1105 78-79 0.19662384056181992 1105 80-81 0.1152599080606116 1105 82-83 0.25656001364584213 1105 84-85 -0.11804543990683314 1105 86-87 0.02895968726596365 1105 88-89 0.09899269728466109 1105 90-91 -0.1675446195967112 1105 92-93 -0.14046928676612325 1105 94-95 0.02433640628963829 1105 96-97 0.23844406234653803 1105 98-99 -0.03264625808648702 1105 100-101 -0.1085114294446008 1105 102-103 0.014907826039145533 1105 104-105 -0.1710198404149743 1105 106-107 -0.05897863756005961 1105 108-109 -0.07264816080862246 1105 110-111 -0.028719811561266795 1105 112-113 -0.0350734799387773 1105 114-115 -0.1587727786247548 1105 116-117 -0.14127437740654614 1105 118-119 0.07744780822013553 1105 120-121 0.052064227424015286 1105 122-123 -0.1358816041190316 1105 124-125 0.11240929493785501 1106 1 0.16081220492100812 1106 2 0.2350083602745272 1106 3 0.009644183909180981 1106 4 -0.13188625712665925 1106 5 0.06642373013880842 1106 6 0.18052532218931105 1106 7 0.2524025089296842 1106 8 0.16415500932308902 1106 9 0.3061955136214536 1106 10-11 0.4147381785769113 1106 12-13 0.31366367812693596 1106 14-15 0.2094852279385222 1106 16-17 0.3770389946023798 1106 18-19 0.16749251186185887 1106 20-21 0.23879217692051213 1106 22-23 0.32528243995324857 1106 24-25 0.41423701793333123 1106 26-27 0.3715248875538535 1106 28-29 0.2567016730733087 1106 30-31 0.16531611951239 1106 32-33 0.2134337167640723 1106 34-35 0.16656973260101182 1106 36-37 0.23249332160009573 1106 38-39 0.19745638570077517 1106 40-41 0.08778519357978354 1106 42-43 0.16841723788073892 1106 44-45 0.11512386807019936 1106 46-47 -0.13944776934095415 1106 48-49 -0.26347475150419086 1106 50-51 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-0.06822192222040613 2314 62-63 -0.09404332522843717 2314 64-65 0.06638578001300388 2314 66-67 -0.10055271559418699 2314 68-69 -0.06946573800198763 2314 70-71 0.10598246569361436 2314 72-73 -0.39872619319172387 2314 74-75 -0.034771610754880555 2314 76-77 -0.12888518806904514 2314 78-79 -0.21394548517417178 2314 80-81 0.02359039239109606 2314 82-83 -0.049674079421585304 2314 84-85 -0.6327534171148095 2314 86-87 -0.395767758128148 2314 88-89 -0.39634728372198325 2314 90-91 -0.06912201750744273 2314 92-93 -0.13687099616783271 2314 94-95 0.017382940002836023 2314 96-97 0.1847308619666741 2314 98-99 0.06157786347096561 2314 100-101 -0.008683794116965515 2314 102-103 0.05892159621958015 2314 104-105 0.09441510735330993 2314 106-107 -0.07217379425521742 2314 108-109 0.02602752770039629 2314 110-111 -0.056016107857566055 2314 112-113 0.06923088853226034 2314 114-115 -0.11587534272731759 2314 116-117 0.013482032849864822 2314 118-119 -0.2624733693677115 2314 120-121 -0.056220672860884946 2314 122-123 -0.33377524134600023 2314 124-125 0.16493113729303133 2315 1 -0.23744386000172568 2315 2 -0.07207961348011338 2315 3 -0.30702679942846345 2315 4 -0.2926390845461526 2315 5 -0.39295639590798004 2315 6 -0.8329426912787028 2315 7 -0.8578452679847643 2315 8 -0.8967963849616396 2315 9 -0.6670411262818519 2315 10-11 -0.8423195784808399 2315 12-13 -0.6475726164426945 2315 14-15 -0.5253416735550474 2315 16-17 -0.7699004856704406 2315 18-19 -0.5570175459815303 2315 20-21 -0.6623128908512186 2315 22-23 -0.9262499782458349 2315 24-25 -0.9162041458411636 2315 26-27 -0.9464842637886335 2315 28-29 -0.6670227506511139 2315 30-31 -0.6577435368806022 2315 32-33 -0.5247170213866639 2315 34-35 -0.6482450822138048 2315 36-37 -0.6473737249336224 2315 38-39 -0.5905087355976804 2315 40-41 -0.4957423899478002 2315 42-43 -1.3395254367286071 2315 44-45 -1.1289407093277077 2315 46-47 -0.4967386266318172 2315 48-49 -0.6809831690126131 2315 50-51 -0.8146008296330223 2315 52-53 -0.4309177307413137 2315 54-55 -0.4067946987652249 2315 56-57 -0.29635998745993675 2315 58-59 -0.45392815975247913 2315 60-61 -0.519788873787359 2315 62-63 -0.6377280355798156 2315 64-65 -0.5470995001389412 2315 66-67 -0.7767160584241992 2315 68-69 -0.580386915589834 2315 70-71 -0.46571743933962395 2315 72-73 -0.7833415778071107 2315 74-75 -0.33106790705117817 2315 76-77 -0.46221852140238084 2315 78-79 -0.48507558963761 2315 80-81 -0.5229433208092864 2315 82-83 -0.9827225124700192 2315 84-85 -0.5130952974566902 2315 86-87 -0.4964325254595856 2315 88-89 -0.524552411927111 2315 90-91 -0.4755797572985152 2315 92-93 -0.6929013855315524 2315 94-95 -0.6896446003580401 2315 96-97 -0.4757629651938231 2315 98-99 -0.5813471127873413 2315 100-101 -0.3795289982955019 2315 102-103 -0.4368048995069138 2315 104-105 -0.5090986628271246 2315 106-107 -0.535611591026349 2315 108-109 -0.39420039252752304 2315 110-111 -0.4586751771833022 2315 112-113 -0.5347577154563403 2315 114-115 -0.7455049723569509 2315 116-117 -0.413868394500561 2315 118-119 -0.5663670065946818 2315 120-121 -0.5543213376282132 2315 122-123 -0.2563773306147574 2315 124-125 -0.3640337250051644 2316 1 -0.4110714068276735 2316 2 -0.25247714591689885 2316 3 -0.48647204327113514 2316 4 -0.5365420349808403 2316 5 -0.37846675706998667 2316 6 -1.0904983149796834 2316 7 -1.4153420944174968 2316 8 -0.8550145044799748 2316 9 -0.7409745412149604 2316 10-11 -1.069432982073856 2316 12-13 -1.169008283232543 2316 14-15 -0.9208227583952251 2316 16-17 -1.0014551361150055 2316 18-19 -2.0844648621439674 2316 20-21 -1.1768339814226039 2316 22-23 -1.1201403043042077 2316 24-25 -1.022785770515398 2316 26-27 -0.7945640917825969 2316 28-29 -0.7256352399597006 2316 30-31 -0.6121756539928853 2316 32-33 -2.5060198395493387 2316 34-35 -1.188189657270307 2316 36-37 -0.5636666838515438 2316 38-39 -0.8391862338918585 2316 40-41 -1.0222417858124189 2316 42-43 -0.8945506811129391 2316 44-45 -0.8681464307474727 2316 46-47 -0.6458792517000731 2316 48-49 -0.46809686964977004 2316 50-51 -0.661495102010278 2316 52-53 -0.35503212419026653 2316 54-55 -0.42555347358042184 2316 56-57 -0.460842069145329 2316 58-59 -0.40084243892679794 2316 60-61 -0.4198090553162821 2316 62-63 -0.12003381457372697 2316 64-65 -0.22388707251069206 2316 66-67 -0.3443416534953627 2316 68-69 -0.4712773578448868 2316 70-71 -0.536652455690156 2316 72-73 -0.5775424023667206 2316 74-75 -0.6089422241772589 2316 76-77 -0.5736153190917257 2316 78-79 -0.55027634343082 2316 80-81 -0.4092221526569588 2316 82-83 -0.27292066712692886 2316 84-85 -0.13760615747813176 2316 86-87 -0.18235770003854768 2316 88-89 -0.19148292369049713 2316 90-91 -0.39322907221860603 2316 92-93 -0.26831565291147896 2316 94-95 -0.2976856728271997 2316 96-97 -0.31099476010056293 2316 98-99 -0.6194705451150071 2316 100-101 -0.2314079335844319 2316 102-103 -0.49215427147695223 2316 104-105 -0.20125259110494298 2316 106-107 -0.3579809374680636 2316 108-109 -0.3052291175703523 2316 110-111 -0.3576958925180307 2316 112-113 -0.41822205399581236 2316 114-115 -0.8544406719090247 2316 116-117 -0.6474643498076489 2316 118-119 -0.7644316766004735 2316 120-121 -0.8620075297056964 2316 122-123 -0.819210030981953 2316 124-125 -0.6729775495883601 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 1.0 14 33.0 15 260.0 16 384.0 17 403.0 18 597.0 19 1801.0 20 6501.0 21 19011.0 22 36167.0 23 56487.0 24 61897.0 25 42296.0 26 25426.0 27 20730.0 28 14935.0 29 12662.0 30 12874.0 31 14492.0 32 18101.0 33 23677.0 34 33640.0 35 48296.0 36 87430.0 37 350828.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.243894254913315 17.23672387506784 13.212956227803687 18.30642564221516 2 14.15584444804435 23.504013895638714 41.189004085893764 21.15113757042317 3 19.366066466725016 35.56201528586181 27.33827112718044 17.733647120232728 4 11.6602375673143 17.65128070133993 36.77771065565564 33.91077107569013 5 11.936314158883867 40.81464598852998 29.64003752843319 17.609002324152968 6 25.09770173129493 36.572283531886654 22.070129256521888 16.25988548029654 7 25.38411348475133 35.31897899722138 21.76886818984622 17.528039328181073 8 29.684564588887763 27.14893186190139 21.863476314220467 21.30302723499038 9 33.04017189204999 13.067058148560628 21.25431698783931 32.638452971550066 10-11 23.936642930264476 27.530289224123383 26.418840628620927 22.114227216991214 12-13 27.723161553778137 25.540649994937738 29.33245587391583 17.4037325773683 14-15 25.30238601464682 23.016997963844172 32.165749834070176 19.51486618743883 16-17 18.915670240638434 27.316247565885615 30.51898524850747 23.249096944968485 18-19 19.512616291496517 24.23503537961369 37.91569639904154 18.336651929848244 20-21 23.47898329643429 22.769857983692425 36.1665721691236 17.58458655074968 22-23 21.168723640973894 25.21078783747147 34.73558571176261 18.88490280979203 24-25 19.217278251247087 26.591904209241868 35.70038609517305 18.490431444337993 26-27 20.227057326449597 25.86785773284342 35.73666492073686 18.16842001997013 28-29 19.886352445847752 26.773070098977364 35.503943844737954 17.836633610436937 30-31 20.726571477126036 26.845633807255247 34.90889017188813 17.518904543730578 32-33 18.29424467798983 27.45107306714561 35.53815406118019 18.716528193684372 34-35 17.635177628183616 28.89080033503577 34.65313362794672 18.820888408833888 36-37 18.821704204056232 29.33373127940601 33.56470518672024 18.279859329817516 38-39 18.84142509982861 28.58712959814059 33.458517919601086 19.112927382429714 40-41 18.232895047279374 29.450829507644716 33.23931702378412 19.076958421291796 42-43 18.59625835520294 29.38959873634768 32.713497459522934 19.30064544892645 44-45 18.91813561544466 28.955722470681522 32.369153679533085 19.75698823434073 46-47 18.108398482018288 28.710689186884753 32.657486663068504 20.52342566802845 48-49 18.079309733467287 29.12503763990083 31.90400207124987 20.891650555382014 50-51 17.133850308372722 28.635494325018882 31.923527617340913 22.30712774926748 52-53 17.512456515462514 27.711406189057623 31.59826675319018 23.177870542289682 54-55 17.8090379632277 27.548385499096113 31.83436028933369 22.8082162483425 56-57 18.32403064736451 26.281471492243885 31.920660855606968 23.473837004784638 58-59 17.772473168634036 26.48233869097606 32.28448790196392 23.460700238425982 60-61 18.361603386214796 25.86182149762329 32.19391933536529 23.582655780796628 62-63 17.70085452284685 25.772692757354577 32.90934605163838 23.6171066681602 64-65 17.271266431584664 25.953447009458625 33.162941095354554 23.612345463602157 66-67 17.72364862392809 25.43781291951267 33.14049470481787 23.698043751741373 68-69 17.95364372230362 25.11219268379309 33.26999247452059 23.664171119382697 70-71 17.40291904244601 25.027608760504556 33.2050928453628 24.364379351686637 72-73 17.966853155673274 25.03738789262918 32.812718189885445 24.183040761812098 74-75 18.171792844824918 24.54629595766648 32.874645181073696 24.407266016434903 76-77 17.72319391634981 24.535079566258272 32.31494155752711 25.426784959864808 78-79 17.893794574838466 24.724027033535357 31.71657998321094 25.665598408415235 80-81 17.62041601173753 25.200529269003447 30.744239530228757 26.43481518903027 82-83 17.744713742132774 24.88839059690779 29.668693337413675 27.698202323545758 84-85 18.45309815754213 24.76827241870831 28.89251147809515 27.88611794565441 86-87 18.33923990819495 24.587327303001665 28.39059898294406 28.68283380585932 88-89 18.69187843498344 24.800053097932498 27.67425993350384 28.833808533580225 90-91 19.241272592991752 25.128654829316144 26.763748165589412 28.866324412102696 92-93 18.984272302185992 26.285802997942987 25.64648032223753 29.083444377633494 94-95 18.760458835990264 26.49134915398388 25.7921715735658 28.956020436460054 96-97 19.220691850856856 26.692867854438845 25.278914962576966 28.807525332127337 98-99 19.652384233543795 27.49528215656772 24.7436052479856 28.108728361902884 100-101 19.56640593799885 27.410371473666327 24.77031090571807 28.252911682616748 102-103 19.351030158625697 28.524107719903952 24.87157117682032 27.253290944650033 104-105 19.2194526550253 28.826760337597833 25.24114733766252 26.712639669714346 106-107 18.745246977189993 28.967283453405752 25.600287093827482 26.687182475576776 108-109 18.5564818371988 29.40421422211373 25.950016790065682 26.089287150621793 110-111 18.35270625624007 29.733072715256004 26.44990367443365 25.464317354070282 112-113 17.648855442728824 30.044115107638504 26.51029418133936 25.796735268293315 114-115 17.79893352532877 29.385033355457807 27.231103261297545 25.584929857915874 116-117 17.618558487203604 29.452468599664282 27.736138174977835 25.19283473815428 118-119 17.478219730530515 29.914195273398846 27.835204071013216 24.772380925057423 120-121 17.370731721036453 30.167314004145585 28.452830613203435 24.009123661614527 122-123 16.944513504605034 30.73086524193993 28.407650190849786 23.916971062605256 124-125 16.388926230932825 31.5130164990587 28.70835088721884 23.389706382789637 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8.0 1 8.0 2 70.5 3 158.5 4 181.0 5 227.5 6 388.5 7 687.0 8 1139.5 9 1451.5 10 1580.0 11 1746.5 12 1946.5 13 2208.0 14 2472.5 15 2794.5 16 3206.0 17 3627.5 18 4169.0 19 4784.5 20 5340.5 21 5966.0 22 6780.0 23 7808.5 24 8872.5 25 10019.0 26 11563.0 27 13473.5 28 15453.0 29 17694.5 30 20106.0 31 22206.0 32 23780.0 33 24307.0 34 23853.0 35 22935.0 36 21832.5 37 20640.5 38 19055.5 39 17045.5 40 15332.0 41 14385.0 42 14046.0 43 14747.5 44 16004.0 45 17610.0 46 21304.0 47 23974.0 48 27805.0 49 33146.5 50 39605.5 51 41219.0 52 36333.5 53 36182.5 54 36731.0 55 32881.5 56 26859.0 57 20971.0 58 16462.5 59 13076.5 60 9951.5 61 7504.0 62 5696.0 63 3900.0 64 2557.5 65 1898.5 66 1463.5 67 1106.0 68 829.5 69 668.0 70 571.0 71 492.5 72 431.5 73 425.0 74 330.0 75 228.5 76 168.5 77 124.5 78 91.5 79 68.5 80 54.0 81 45.0 82 35.5 83 18.0 84 6.0 85 2.5 86 2.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09359567120020698 2 0.0020249063480814013 3 4.499791884625336E-4 4 3.374843913469002E-4 5 4.499791884625336E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.374843913469002E-4 18-19 0.0 20-21 0.0020249063480814013 22-23 3.374843913469002E-4 24-25 0.01794292013994353 26-27 0.23685779532696613 28-29 0.04426670266500174 30-31 0.04353548648375012 32-33 0.03476089230873072 34-35 0.2764559639116691 36-37 0.11671335200746966 38-39 0.10214527578099512 40-41 0.10838873702091278 42-43 0.07998380074921535 44-45 0.05112888528905538 46-47 0.057766078318877756 48-49 0.06496574533427829 50-51 0.06265960199340781 52-53 0.06069094304388422 54-55 0.06119716963090457 56-57 0.06372830256600633 58-59 0.06794685745784258 60-61 0.0700280112044818 62-63 0.07385283430641333 64-65 0.06609069330543461 66-67 0.0700280112044818 68-69 0.06912805282755673 70-71 0.07030924819727088 72-73 0.10597009888292667 74-75 0.0306548322140101 76-77 0.14680571023590158 78-79 0.02879866806160215 80-81 0.017661683147154442 82-83 0.013049396465413476 84-85 0.007087172218284904 86-87 0.010124531740407006 88-89 7.31216181251617E-4 90-91 0.0023061433408704847 92-93 0.0023061433408704847 94-95 0.003206101717795552 96-97 0.004387297087509703 98-99 0.0019686589495235846 100-101 0.0012374427682719674 102-103 0.001518679761061051 104-105 2.812369927890835E-4 106-107 0.0024748855365439347 108-109 0.001293690166829784 110-111 0.0019686589495235846 112-113 6.187213841359837E-4 114-115 0.002249895942312668 116-117 0.009674552551944472 118-119 0.012318180284161859 120-121 0.0036560809062580854 122-123 0.0012374427682719674 124-125 5.62473985578167E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 888930.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.64265190336361 #Duplication Level Percentage of deduplicated Percentage of total 1 75.51056763988643 31.44460283253183 2 14.308595776517915 11.916957462949487 3 4.2375172425812755 5.293843664019398 4 1.7222926847459032 2.8688333898633283 5 0.8906826647308828 1.8545194081874234 6 0.5807966109430436 1.451154665769268 7 0.4006373123948786 1.1678520097691352 8 0.2572288340556542 0.8569352636870154 9 0.2273856409533276 0.852204698463838 >10 1.5330204790508886 12.44615508841475 >50 0.1687375367240311 4.834038405077438 >100 0.13827823750321167 11.14489511224512 >500 0.01671198971755988 4.753212625975007 >1k 0.0067387055312741456 4.91383812230926 >5k 5.390964425019317E-4 1.9408633522820067 >10k+ 2.6954822125096583E-4 2.2600938984557404 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20135 2.2650827399232782 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9800 1.1024490117332075 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7491 0.8426985251932098 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3512 0.39508172747010456 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3502 0.3939567794989482 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2539 0.2856242898765932 No Hit ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC 2342 0.26346281484481343 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2267 0.2550257050611409 No Hit GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC 2121 0.23860146468225843 No Hit GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA 2065 0.232301756043783 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2062 0.2319642716524361 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1830 0.20586547872160915 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1821 0.20485302554756843 No Hit GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA 1818 0.20451554115622153 No Hit GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT 1784 0.20069071805428998 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 1564 0.17594186268885065 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1383 0.155580304410921 No Hit GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1380 0.1552428200195741 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1350 0.15186797610610508 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 1298 0.14601824665609217 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1272 0.1430933819310857 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 1222 0.13746864207530402 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA 1157 0.13015648026278784 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 1140 0.12824406871182206 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 1119 0.12588167797239377 No Hit ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT 1118 0.12576918317527816 No Hit GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA 1107 0.12453174040700618 No Hit TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT 1004 0.11294477630409594 No Hit GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT 993 0.11170733353582397 No Hit ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG 962 0.10821999482523934 No Hit TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA 952 0.107095046854083 No Hit CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC 951 0.10698255205696737 No Hit GTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTACACGGTGGA 900 0.10124531740407007 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 1.124947971156334E-4 0.0 4 0.0 0.0 0.0 1.124947971156334E-4 0.0 5 0.0 0.0 0.0 1.124947971156334E-4 0.0 6 0.0 0.0 0.0 1.124947971156334E-4 0.0 7 0.0 0.0 0.0 2.249895942312668E-4 0.0 8 0.0 0.0 0.0 2.249895942312668E-4 0.0 9 0.0 0.0 0.0 4.499791884625336E-4 0.0 10-11 0.0 0.0 0.0 5.62473985578167E-4 0.0 12-13 0.0 0.0 0.0 8.437109783672505E-4 0.0 14-15 0.0 0.0 0.0 8.999583769250672E-4 0.0 16-17 0.0 0.0 0.0 0.0015749271596188674 0.0 18-19 0.0 0.0 0.0 0.0030373595221221015 0.0 20-21 0.0 0.0 0.0 0.0071996670154005375 0.0 22-23 0.0 0.0 0.0 0.023792649589956465 0.0 24-25 1.687421956734501E-4 0.0 0.0 0.060747190442442034 0.0 26-27 3.374843913469002E-4 0.0 0.0 0.11013240637620511 0.0 28-29 3.374843913469002E-4 0.0 0.0 0.2027718718009292 0.0 30-31 3.374843913469002E-4 0.0 0.0 0.38928824541864937 0.0 32-33 3.374843913469002E-4 0.0 0.0 0.7131607663145579 0.0 34-35 3.374843913469002E-4 0.0 0.0 0.9709988413035897 1.124947971156334E-4 36-37 3.374843913469002E-4 0.0 0.0 1.293690166829784 1.124947971156334E-4 38-39 3.374843913469002E-4 0.0 0.0 1.7332635865591217 1.124947971156334E-4 40-41 3.374843913469002E-4 0.0 0.0 2.3620532550369546 1.124947971156334E-4 42-43 3.374843913469002E-4 0.0 0.0 2.920983654505979 1.124947971156334E-4 44-45 3.374843913469002E-4 0.0 0.0 3.4141608450609158 1.124947971156334E-4 46-47 3.374843913469002E-4 0.0 0.0 3.908294241391336 1.124947971156334E-4 48-49 3.374843913469002E-4 0.0 0.0 4.376778823979391 1.124947971156334E-4 50-51 3.374843913469002E-4 0.0 0.0 4.864781253867008 1.124947971156334E-4 52-53 3.374843913469002E-4 0.0 0.0 5.420618046415353 1.124947971156334E-4 54-55 3.374843913469002E-4 0.0 0.0 5.947318686510749 1.124947971156334E-4 56-57 3.374843913469002E-4 0.0 0.0 6.412147188192547 1.124947971156334E-4 58-59 3.374843913469002E-4 0.0 0.0 6.9426726513898736 1.124947971156334E-4 60-61 3.374843913469002E-4 0.0 0.0 7.465829705376127 2.249895942312668E-4 62-63 3.374843913469002E-4 0.0 0.0 8.01086699740137 2.249895942312668E-4 64-65 3.374843913469002E-4 0.0 0.0 8.559841607325662 2.249895942312668E-4 66-67 3.374843913469002E-4 0.0 0.0 9.055437436018584 2.249895942312668E-4 68-69 3.374843913469002E-4 0.0 0.0 9.573925955924539 2.249895942312668E-4 70-71 3.374843913469002E-4 0.0 0.0 10.141855939162813 2.249895942312668E-4 72-73 3.374843913469002E-4 0.0 0.0 10.702136276197226 2.249895942312668E-4 74-75 3.374843913469002E-4 0.0 0.0 11.212075191522391 2.812369927890835E-4 76-77 3.374843913469002E-4 0.0 0.0 11.73511975071153 3.374843913469002E-4 78-79 3.374843913469002E-4 0.0 0.0 12.265420224314624 3.374843913469002E-4 80-81 3.374843913469002E-4 0.0 0.0 12.800164242403788 3.374843913469002E-4 82-83 3.374843913469002E-4 0.0 0.0 13.382662301868539 3.374843913469002E-4 84-85 3.374843913469002E-4 0.0 0.0 13.906438077238928 3.374843913469002E-4 86-87 3.374843913469002E-4 0.0 0.0 14.423070432992475 3.374843913469002E-4 88-89 3.374843913469002E-4 0.0 0.0 14.935146749462838 3.374843913469002E-4 90-91 3.374843913469002E-4 0.0 0.0 15.458978772231784 3.374843913469002E-4 92-93 3.374843913469002E-4 0.0 0.0 15.966724039013194 3.374843913469002E-4 94-95 3.374843913469002E-4 0.0 0.0 16.462601104698905 4.499791884625336E-4 96-97 3.374843913469002E-4 0.0 0.0 16.979514697445243 4.499791884625336E-4 98-99 3.374843913469002E-4 0.0 0.0 17.485685037067036 4.499791884625336E-4 100-101 3.374843913469002E-4 0.0 0.0 18.030947318686508 4.499791884625336E-4 102-103 3.374843913469002E-4 0.0 0.0 18.541729944990045 6.187213841359837E-4 104-105 3.374843913469002E-4 0.0 0.0 19.042219297357498 7.874635798094338E-4 106-107 3.374843913469002E-4 0.0 0.0 19.54141495955812 7.874635798094338E-4 108-109 3.374843913469002E-4 0.0 0.0 20.069409289820346 8.437109783672505E-4 110-111 3.374843913469002E-4 0.0 0.0 20.577435793594546 0.0010124531740407007 112-113 3.374843913469002E-4 0.0 0.0 21.110773626719762 0.0010124531740407007 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 290 0.0 75.889725 1 TACAACG 125 0.0 66.56267 2 TAGTACT 335 0.0 63.866104 5 ATAGTAC 365 0.0 58.61683 4 GCATAGT 380 0.0 56.30301 2 ATAACGC 230 0.0 54.263042 3 CATAGTA 395 0.0 54.164917 3 GTATAAC 220 0.0 51.370075 1 AGATTAC 310 0.0 46.01106 2 ATCTACG 125 6.311893E-10 42.790287 2 GTACAAC 225 0.0 42.2977 1 ACAACGC 255 0.0 39.620636 3 TACTGGT 755 0.0 39.358246 2 TAACGCA 275 0.0 38.90026 4 TCACGCA 155 1.2187229E-10 38.34255 4 ACTGGTT 820 0.0 36.963154 3 GTACTGG 890 0.0 35.421352 1 GGTTCAC 840 0.0 35.37557 6 GATTACT 405 0.0 35.21834 3 AACGCAG 22700 0.0 35.108772 6 >>END_MODULE