FastQCFastQC Report
Fri 10 Feb 2017
SRR3556625.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556625.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140985
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142141.2457657199700258No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75120.6583785062906173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58610.5136789703633264No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28850.2528517026954781No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA26990.2365499984662375No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21100.1849279350736425No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18290.16030009158753183No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16930.14838056591453874No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA14540.12743375241567595No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14520.12725846527342602No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13440.11779295959193153No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12210.1070128003435628No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11540.10114068107819121No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3100.059.5022771
TAACGCA2700.046.2329064
ACGCATA1256.2391337E-1042.8416441
TACAACG2800.042.4587942
TATCGCT600.001314763839.62827
ACAACGC3100.038.3498763
TAGTACT5500.037.8269235
ATAGTAC5250.037.3637354
GTATAAC3800.036.0145381
CATGGGT19150.033.8340034
CATGGGG40000.033.2876934
GTACTGG7000.033.151271
ACATGGG161450.032.7310073
AACGCAG161650.032.4331056
TACATGG168550.032.304452
GTACATG169500.032.2260131
GTACAAC4500.031.734551
ATGGGTG11250.031.7025625
GCATAGT6000.031.7025622
GTATCAA162600.031.0685351
Produced by FastQC (version 0.11.5)