FastQCFastQC Report
Fri 10 Feb 2017
SRR3556609.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556609.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1934367
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312751.6168079790443075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154660.7995380400927022No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109950.5684029969493897No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60020.31028238178174045No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA42940.22198476297414088No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.17669863061146102No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33370.17251121426285704No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32680.16894415589182404No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC30850.15948369673386695No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC29870.15441743991703746No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT28620.14795537765067332No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28620.14795537765067332No Hit
GTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAAAAAAAAAAAAAAA28040.14495698075908037No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA24570.1270182959076535No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24060.12438177450297694No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA21860.11300854491417606No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19950.10313451377117165No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT19400.10029120637397143No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG3450.049.961762
CGCATAG5400.042.9591181
ACAACGC4250.039.158673
AACGCAG308750.038.1746066
GTATCAA310450.037.0935331
AGATTAC8600.035.247682
CGCAGAG346500.034.0156148
ACGCAGA346500.033.9813087
CATGGGG70050.033.8550574
CATGGGC67650.033.5625084
ACATGGG283600.032.883313
TAACGCA4350.032.7929734
GCAGAGT362250.032.6023039
TATCAAC352000.032.5554122
ATCAACG353650.032.470753
ATGGGAC22700.032.2060975
TCAACGC361800.032.150014
GTACTGG16750.031.9604021
CAACGCA366200.031.844875
GTACATG307450.031.7094671