FastQCFastQC Report
Fri 10 Feb 2017
SRR3556605.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556605.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5872047
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT793701.3516581185402639No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT406750.6926885973494422No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271460.46229194010197805No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146610.24967443210178664No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA137310.2338366842091012No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT115560.1967967899439497No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC88970.15151445484002427No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC88180.1501690977609682No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80220.13661334795174496No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA77590.13213450096703924No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75840.12915427958938341No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT67550.11503654517751646No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64770.11030225064615456No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG60730.10342219672288046No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA60200.10251961539136181No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59950.10209386948026812No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCAG783150.036.8059736
GTATCAA781950.036.3428961
CGCATAG10700.035.026761
TAACGCA9250.033.4105074
ACGCAGA871050.033.0371977
CGCAGAG873450.033.014468
CATGGGG185200.032.0907944
ACATGGG722150.031.8581773
CATGGGT66300.031.8226454
TATCAAC892500.031.6984982
ATCAACG896000.031.6266633
GCAGAGT918000.031.418769
TCAACGC915150.031.1594264
TACTGGT37600.031.139982
CAACGCA922650.030.9576665
TACATGG771400.030.9115832
GTACATG775600.030.8111041
CATGGGC171750.030.1745784
GACCGTT9650.028.3303537
GTACTGG45850.028.2852751
Produced by FastQC (version 0.11.5)