FastQCFastQC Report
Fri 10 Feb 2017
SRR3556601.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3556601.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3498210
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401871.148787522761641No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT196060.5604580628378514No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155750.44522770216768004No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70840.20250356610952458No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGT37050.1059113089265653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA277050.054.9858171
AACGCAG341050.045.2551656
TATCAAC345850.044.0771832
ATCAACG349450.043.7081383
TCAACGC354000.043.3478164
CAACGCA360700.042.4767235
ACGCAGA394500.039.033247
GGTATCA146200.038.5709421
CGCAGAG400350.038.4777268
GCAGAGT421850.036.4744079
GTACATG323400.030.5144561
TACATGG325400.030.3548242
ACATGGG328350.029.8649063
AGAGTAC409500.029.51965510-11
CATGGGG112350.028.5121484
AGTACTT238500.027.31092612-13
GGACCGT11400.027.1089526
GAGTACT216900.026.03024512-13
CATGGGA151200.025.8242574
GTACTGG24800.025.1847061
Produced by FastQC (version 0.11.5)